Streptomyces phage SV1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Picardvirus; unclassified Picardvirus

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4IBH2|K4IBH2_9CAUD Tail assembly chaperone OS=Streptomyces phage SV1 OX=1204525 GN=SV1_14 PE=4 SV=1
MM1 pKa = 7.91DD2 pKa = 3.94RR3 pKa = 11.84RR4 pKa = 11.84VYY6 pKa = 9.23ATPAQLAQWTGEE18 pKa = 4.06PAPADD23 pKa = 3.55AEE25 pKa = 4.23RR26 pKa = 11.84LLTRR30 pKa = 11.84ASEE33 pKa = 4.36DD34 pKa = 3.09VDD36 pKa = 4.52DD37 pKa = 5.31ALLTAVYY44 pKa = 10.22DD45 pKa = 3.62VDD47 pKa = 3.97EE48 pKa = 6.5AGMPTDD54 pKa = 3.67PAVAQALADD63 pKa = 3.77AACAQVAYY71 pKa = 9.66RR72 pKa = 11.84QEE74 pKa = 4.31SGDD77 pKa = 3.8TGTGAAGRR85 pKa = 11.84WSSVSIGPVSMSGPRR100 pKa = 11.84QSAGGTGAGSVDD112 pKa = 3.96LGEE115 pKa = 4.15QASRR119 pKa = 11.84ALARR123 pKa = 11.84AGLTPGEE130 pKa = 4.29IYY132 pKa = 10.45PPGVNWW138 pKa = 3.53

Molecular weight: 14.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4I2W9|K4I2W9_9CAUD Uncharacterized protein OS=Streptomyces phage SV1 OX=1204525 GN=SV1_20 PE=4 SV=1
MM1 pKa = 7.62AAHH4 pKa = 6.58TAPRR8 pKa = 11.84HH9 pKa = 4.82RR10 pKa = 11.84PRR12 pKa = 11.84RR13 pKa = 11.84NRR15 pKa = 11.84GAHH18 pKa = 4.75RR19 pKa = 11.84QPRR22 pKa = 11.84RR23 pKa = 11.84LNHH26 pKa = 5.09TPGRR30 pKa = 11.84GRR32 pKa = 11.84MTPDD36 pKa = 2.7GRR38 pKa = 4.02

Molecular weight: 4.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11832

38

805

215.1

23.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.382 ± 0.549

0.701 ± 0.123

6.525 ± 0.34

5.722 ± 0.29

1.53 ± 0.176

8.3 ± 0.457

2.104 ± 0.218

3.524 ± 0.264

1.927 ± 0.253

8.122 ± 0.362

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.8 ± 0.18

1.758 ± 0.152

6.939 ± 0.463

3.829 ± 0.259

9.407 ± 0.526

4.437 ± 0.228

7.361 ± 0.359

6.795 ± 0.341

1.893 ± 0.138

1.944 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski