Actinokineospora bangkokensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Actinokineospora

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6172 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9LI70|A0A1Q9LI70_9PSEU ABC transporter ATP-binding protein OS=Actinokineospora bangkokensis OX=1193682 GN=BJP25_26110 PE=4 SV=1
MM1 pKa = 6.7VAEE4 pKa = 5.01AKK6 pKa = 10.47RR7 pKa = 11.84LVRR10 pKa = 11.84LIGVAAAVFAAGAAATVSTAQADD33 pKa = 4.01TVTPYY38 pKa = 10.45IVGGEE43 pKa = 4.14GADD46 pKa = 3.15IADD49 pKa = 3.81YY50 pKa = 10.14PFTVALTTPDD60 pKa = 3.26GFQFCGGSLARR71 pKa = 11.84ANKK74 pKa = 9.43VVTAAHH80 pKa = 6.23CVDD83 pKa = 3.67GSSPAEE89 pKa = 3.87VQVVSGRR96 pKa = 11.84TDD98 pKa = 3.27LTAAGGEE105 pKa = 4.35TAGVTGIWVHH115 pKa = 6.74PDD117 pKa = 3.01YY118 pKa = 11.16SDD120 pKa = 3.73PSISADD126 pKa = 3.56VAVLTLDD133 pKa = 3.66TSLSATPIDD142 pKa = 3.75VAGAGDD148 pKa = 3.61EE149 pKa = 4.11GLYY152 pKa = 10.98AEE154 pKa = 4.86GTTTTVLGWGATSEE168 pKa = 4.33GGSTSDD174 pKa = 3.09TLLKK178 pKa = 10.48VDD180 pKa = 4.06VPVVSDD186 pKa = 3.82SDD188 pKa = 3.81CTTAYY193 pKa = 10.51SSDD196 pKa = 4.02YY197 pKa = 10.56IADD200 pKa = 3.73SMVCAGVPDD209 pKa = 4.58GGLDD213 pKa = 3.45SCQGDD218 pKa = 3.21SGGPLVAGGKK228 pKa = 10.2LIGAVSWGEE237 pKa = 3.86GCARR241 pKa = 11.84PGKK244 pKa = 9.15PGVYY248 pKa = 8.89TRR250 pKa = 11.84LASFSSDD257 pKa = 2.74IEE259 pKa = 4.19AQLGG263 pKa = 3.6

Molecular weight: 25.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9LGR4|A0A1Q9LGR4_9PSEU ATP synthase subunit alpha OS=Actinokineospora bangkokensis OX=1193682 GN=atpA PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84DD41 pKa = 3.0KK42 pKa = 11.37LSAA45 pKa = 3.85

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6172

0

6172

2152045

31

7685

348.7

36.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.746 ± 0.066

0.729 ± 0.008

6.199 ± 0.025

5.246 ± 0.031

2.703 ± 0.016

9.648 ± 0.033

2.271 ± 0.018

2.535 ± 0.023

1.66 ± 0.022

10.629 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.412 ± 0.014

1.548 ± 0.019

6.229 ± 0.029

2.609 ± 0.023

8.011 ± 0.037

4.644 ± 0.023

6.214 ± 0.048

9.754 ± 0.039

1.487 ± 0.014

1.727 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski