Pseudomonas phage vB_PaeP_MAG4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Migulavirinae; Litunavirus; unclassified Litunavirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172B2K7|A0A172B2K7_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_MAG4 OX=1639814 GN=vB_PaeP_fi6_078 PE=4 SV=1
MM1 pKa = 7.89TSTCWVNDD9 pKa = 3.36HH10 pKa = 7.09EE11 pKa = 4.74ITVSQGVIAVRR22 pKa = 11.84KK23 pKa = 10.18DD24 pKa = 3.01GVLVHH29 pKa = 6.46EE30 pKa = 5.47CGGLWAACDD39 pKa = 3.69FARR42 pKa = 11.84GINSAEE48 pKa = 4.19SPGPEE53 pKa = 3.41VDD55 pKa = 3.63EE56 pKa = 4.42VQEE59 pKa = 4.37TVHH62 pKa = 6.58VDD64 pKa = 3.12SDD66 pKa = 3.67EE67 pKa = 4.61CGQAEE72 pKa = 4.49CSS74 pKa = 3.53

Molecular weight: 7.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172B091|A0A172B091_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeP_MAG4 OX=1639814 GN=vB_PaeP_fi6_053 PE=4 SV=1
MM1 pKa = 7.53AKK3 pKa = 9.98KK4 pKa = 10.45AKK6 pKa = 10.09LKK8 pKa = 10.82ADD10 pKa = 3.4WTPGKK15 pKa = 10.23LGHH18 pKa = 6.75APVNGRR24 pKa = 11.84YY25 pKa = 9.03FFVNRR30 pKa = 11.84EE31 pKa = 3.58TRR33 pKa = 11.84MTHH36 pKa = 4.41TWTIGQLPVPEE47 pKa = 5.33GYY49 pKa = 10.06EE50 pKa = 3.67QVTMQEE56 pKa = 3.75YY57 pKa = 10.7DD58 pKa = 3.15SFRR61 pKa = 11.84KK62 pKa = 9.93FNGTLSKK69 pKa = 10.38KK70 pKa = 10.51KK71 pKa = 10.37LMAFIRR77 pKa = 11.84GEE79 pKa = 4.13AKK81 pKa = 10.2CSKK84 pKa = 10.48DD85 pKa = 3.11AGKK88 pKa = 10.58KK89 pKa = 9.89SEE91 pKa = 4.45SEE93 pKa = 4.15SKK95 pKa = 10.25PAAKK99 pKa = 10.22SKK101 pKa = 9.29TRR103 pKa = 11.84ASSSTVSQVPATSGKK118 pKa = 10.42ARR120 pKa = 11.84IQVRR124 pKa = 11.84AGAVVTVAA132 pKa = 4.6

Molecular weight: 14.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

22770

50

3398

244.8

27.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.196 ± 0.481

0.935 ± 0.156

5.986 ± 0.198

6.917 ± 0.268

3.86 ± 0.215

6.728 ± 0.208

1.924 ± 0.179

4.989 ± 0.224

5.885 ± 0.256

8.964 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.074 ± 0.143

4.545 ± 0.148

3.988 ± 0.2

4.387 ± 0.253

5.446 ± 0.199

5.613 ± 0.211

5.867 ± 0.202

7.207 ± 0.223

1.318 ± 0.141

3.171 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski