Enterococcus phage vB_EfaP_Ef7.2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Rountreeviridae; Sarlesvirinae; Copernicusvirus; Enterococcus virus Ef72

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6DSE4|A0A4D6DSE4_9CAUD Tail tube protein OS=Enterococcus phage vB_EfaP_Ef7.2 OX=2546618 PE=4 SV=1
MM1 pKa = 7.88PSYY4 pKa = 9.55KK5 pKa = 10.19TYY7 pKa = 8.3TTEE10 pKa = 3.54QYY12 pKa = 10.69QAFLSQPFGYY22 pKa = 10.5DD23 pKa = 2.99FGISDD28 pKa = 3.68EE29 pKa = 5.22TIAQWFMIQPGARR42 pKa = 11.84PVINSYY48 pKa = 10.97GVTKK52 pKa = 10.85ANLLSEE58 pKa = 5.04YY59 pKa = 10.08IPKK62 pKa = 10.37LKK64 pKa = 10.6QEE66 pKa = 4.42FGGSLVFLMTTVSEE80 pKa = 4.46GGGAGNWVNHH90 pKa = 5.17YY91 pKa = 10.17MSDD94 pKa = 3.44TSDD97 pKa = 2.87TGMGCMIDD105 pKa = 4.33DD106 pKa = 3.62IDD108 pKa = 4.31YY109 pKa = 10.99IKK111 pKa = 8.47TTFDD115 pKa = 2.71RR116 pKa = 11.84HH117 pKa = 5.38FPPAISAPEE126 pKa = 3.73VGGAYY131 pKa = 9.33TEE133 pKa = 4.56DD134 pKa = 3.83EE135 pKa = 4.7EE136 pKa = 4.86GLTMKK141 pKa = 10.33VYY143 pKa = 10.83NAVPDD148 pKa = 4.24GSIGSYY154 pKa = 9.63FIPSTMAGNAWIFGSQWCLANQGAAPPAVYY184 pKa = 10.15FGNPYY189 pKa = 10.29DD190 pKa = 3.84QLIDD194 pKa = 3.81IIKK197 pKa = 10.65SFGADD202 pKa = 3.34PFKK205 pKa = 10.91EE206 pKa = 4.26GSTAKK211 pKa = 10.24PNPDD215 pKa = 3.49TPKK218 pKa = 10.8GDD220 pKa = 3.97PNEE223 pKa = 4.66SGNKK227 pKa = 8.93PKK229 pKa = 10.43PKK231 pKa = 9.78PDD233 pKa = 3.01IQKK236 pKa = 10.85AIDD239 pKa = 4.27KK240 pKa = 10.73ILDD243 pKa = 4.43EE244 pKa = 4.75INKK247 pKa = 9.98ALDD250 pKa = 3.31NQTIQGSPLLSYY262 pKa = 11.17NDD264 pKa = 3.78DD265 pKa = 3.49VTIEE269 pKa = 4.01RR270 pKa = 11.84TFNNSYY276 pKa = 10.08KK277 pKa = 10.11ISYY280 pKa = 8.6TSSFKK285 pKa = 10.9KK286 pKa = 10.36KK287 pKa = 10.6LSDD290 pKa = 3.39MLNASDD296 pKa = 5.62LGLITDD302 pKa = 5.61DD303 pKa = 4.39GAQTNPPKK311 pKa = 10.21EE312 pKa = 4.25DD313 pKa = 3.69PKK315 pKa = 10.82PPSIEE320 pKa = 3.83GGKK323 pKa = 10.07DD324 pKa = 2.86SKK326 pKa = 9.77TMKK329 pKa = 10.35KK330 pKa = 10.01IYY332 pKa = 9.75DD333 pKa = 3.66WCNQNQGQAFDD344 pKa = 3.5TDD346 pKa = 3.98GYY348 pKa = 11.55YY349 pKa = 10.79GAQCVDD355 pKa = 5.11LISWINTKK363 pKa = 10.03VFGLGLDD370 pKa = 3.55TSGDD374 pKa = 3.6YY375 pKa = 11.17AKK377 pKa = 10.76NIWNNPVPSGWYY389 pKa = 9.28KK390 pKa = 11.25VNGNPNDD397 pKa = 3.97DD398 pKa = 3.77NASRR402 pKa = 11.84EE403 pKa = 3.99IWNTLPNGAIVWWTNSGAGHH423 pKa = 5.99VGIKK427 pKa = 10.48AGDD430 pKa = 3.47FATTLQQNWTSHH442 pKa = 5.43GLGGPIVLADD452 pKa = 3.69CASWMVSSGSGFLGAWVTDD471 pKa = 3.66NN472 pKa = 4.3

Molecular weight: 51.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6DSY0|A0A4D6DSY0_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaP_Ef7.2 OX=2546618 PE=4 SV=1
MM1 pKa = 8.12RR2 pKa = 11.84YY3 pKa = 9.46KK4 pKa = 10.05QVKK7 pKa = 9.64RR8 pKa = 11.84LWEE11 pKa = 3.86LFGEE15 pKa = 4.71GEE17 pKa = 4.16YY18 pKa = 11.31GLNSFDD24 pKa = 3.57EE25 pKa = 5.0FTFNLHH31 pKa = 5.92NEE33 pKa = 4.58GIISFKK39 pKa = 10.79QYY41 pKa = 11.19LKK43 pKa = 10.66FIKK46 pKa = 9.97KK47 pKa = 9.42LKK49 pKa = 10.02KK50 pKa = 10.07KK51 pKa = 10.27EE52 pKa = 4.18GYY54 pKa = 9.12KK55 pKa = 9.94QQ56 pKa = 3.07

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30

0

30

5911

48

782

197.0

22.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.211 ± 0.328

0.812 ± 0.143

6.073 ± 0.317

7.714 ± 0.598

4.838 ± 0.373

5.87 ± 0.538

1.726 ± 0.164

6.902 ± 0.292

8.239 ± 0.414

7.867 ± 0.468

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.876 ± 0.206

7.105 ± 0.406

3.265 ± 0.307

3.316 ± 0.202

3.282 ± 0.249

5.617 ± 0.36

6.953 ± 0.443

5.87 ± 0.314

1.286 ± 0.182

5.177 ± 0.285
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski