Amino acid dipepetide frequency for Bacillus phage vB_BspS_SplendidRed

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.607AlaAla: 7.607 ± 0.977
0.217AlaCys: 0.217 ± 0.119
4.347AlaAsp: 4.347 ± 0.599
5.651AlaGlu: 5.651 ± 0.83
3.26AlaPhe: 3.26 ± 0.554
3.84AlaGly: 3.84 ± 0.459
1.014AlaHis: 1.014 ± 0.245
4.347AlaIle: 4.347 ± 0.747
6.448AlaLys: 6.448 ± 0.825
5.941AlaLeu: 5.941 ± 0.725
2.536AlaMet: 2.536 ± 0.459
2.753AlaAsn: 2.753 ± 0.421
1.594AlaPro: 1.594 ± 0.309
2.318AlaGln: 2.318 ± 0.493
3.84AlaArg: 3.84 ± 0.559
3.478AlaSer: 3.478 ± 0.5
3.405AlaThr: 3.405 ± 0.608
5.578AlaVal: 5.578 ± 0.71
1.087AlaTrp: 1.087 ± 0.317
2.101AlaTyr: 2.101 ± 0.346
0.0AlaXaa: 0.0 ± 0.0
Cys
0.362CysAla: 0.362 ± 0.137
0.29CysCys: 0.29 ± 0.187
0.724CysAsp: 0.724 ± 0.213
0.507CysGlu: 0.507 ± 0.205
0.362CysPhe: 0.362 ± 0.189
0.942CysGly: 0.942 ± 0.362
0.145CysHis: 0.145 ± 0.15
0.652CysIle: 0.652 ± 0.243
0.797CysLys: 0.797 ± 0.229
0.362CysLeu: 0.362 ± 0.163
0.217CysMet: 0.217 ± 0.118
0.507CysAsn: 0.507 ± 0.202
0.435CysPro: 0.435 ± 0.184
0.29CysGln: 0.29 ± 0.154
0.58CysArg: 0.58 ± 0.202
0.217CysSer: 0.217 ± 0.13
0.507CysThr: 0.507 ± 0.18
0.29CysVal: 0.29 ± 0.145
0.0CysTrp: 0.0 ± 0.0
0.145CysTyr: 0.145 ± 0.094
0.0CysXaa: 0.0 ± 0.0
Asp
4.057AspAla: 4.057 ± 0.532
0.652AspCys: 0.652 ± 0.18
4.274AspAsp: 4.274 ± 0.682
4.926AspGlu: 4.926 ± 0.668
2.608AspPhe: 2.608 ± 0.485
5.578AspGly: 5.578 ± 0.636
0.942AspHis: 0.942 ± 0.265
3.84AspIle: 3.84 ± 0.431
3.985AspLys: 3.985 ± 0.51
5.216AspLeu: 5.216 ± 0.636
1.739AspMet: 1.739 ± 0.316
3.043AspAsn: 3.043 ± 0.522
2.101AspPro: 2.101 ± 0.468
1.449AspGln: 1.449 ± 0.378
3.622AspArg: 3.622 ± 0.535
4.057AspSer: 4.057 ± 0.505
3.115AspThr: 3.115 ± 0.462
3.695AspVal: 3.695 ± 0.452
0.362AspTrp: 0.362 ± 0.16
1.884AspTyr: 1.884 ± 0.387
0.0AspXaa: 0.0 ± 0.0
Glu
6.231GluAla: 6.231 ± 0.744
0.869GluCys: 0.869 ± 0.292
3.695GluAsp: 3.695 ± 0.665
6.231GluGlu: 6.231 ± 0.732
2.825GluPhe: 2.825 ± 0.461
4.709GluGly: 4.709 ± 0.537
1.087GluHis: 1.087 ± 0.251
4.999GluIle: 4.999 ± 0.678
7.317GluLys: 7.317 ± 0.865
6.593GluLeu: 6.593 ± 0.809
2.753GluMet: 2.753 ± 0.46
4.202GluAsn: 4.202 ± 0.545
1.884GluPro: 1.884 ± 0.354
2.753GluGln: 2.753 ± 0.435
4.202GluArg: 4.202 ± 0.647
3.622GluSer: 3.622 ± 0.524
4.419GluThr: 4.419 ± 0.65
5.578GluVal: 5.578 ± 0.839
1.304GluTrp: 1.304 ± 0.341
2.753GluTyr: 2.753 ± 0.498
0.0GluXaa: 0.0 ± 0.0
Phe
2.173PheAla: 2.173 ± 0.414
0.362PheCys: 0.362 ± 0.16
2.898PheAsp: 2.898 ± 0.429
2.608PheGlu: 2.608 ± 0.43
1.956PhePhe: 1.956 ± 0.325
2.536PheGly: 2.536 ± 0.462
0.58PheHis: 0.58 ± 0.173
3.115PheIle: 3.115 ± 0.481
3.478PheLys: 3.478 ± 0.468
2.681PheLeu: 2.681 ± 0.529
1.304PheMet: 1.304 ± 0.271
2.681PheAsn: 2.681 ± 0.429
0.869PhePro: 0.869 ± 0.261
0.724PheGln: 0.724 ± 0.215
0.724PheArg: 0.724 ± 0.264
2.681PheSer: 2.681 ± 0.403
3.043PheThr: 3.043 ± 0.578
2.029PheVal: 2.029 ± 0.334
0.58PheTrp: 0.58 ± 0.193
1.739PheTyr: 1.739 ± 0.368
0.0PheXaa: 0.0 ± 0.0
Gly
4.999GlyAla: 4.999 ± 0.571
0.869GlyCys: 0.869 ± 0.302
3.767GlyAsp: 3.767 ± 0.618
5.434GlyGlu: 5.434 ± 0.75
2.536GlyPhe: 2.536 ± 0.448
5.434GlyGly: 5.434 ± 0.649
1.014GlyHis: 1.014 ± 0.277
5.578GlyIle: 5.578 ± 1.006
6.81GlyLys: 6.81 ± 0.72
4.854GlyLeu: 4.854 ± 0.634
2.029GlyMet: 2.029 ± 0.365
3.622GlyAsn: 3.622 ± 0.449
1.304GlyPro: 1.304 ± 0.306
2.825GlyGln: 2.825 ± 0.438
3.84GlyArg: 3.84 ± 0.572
3.767GlySer: 3.767 ± 0.559
3.622GlyThr: 3.622 ± 0.473
4.999GlyVal: 4.999 ± 0.547
0.507GlyTrp: 0.507 ± 0.155
3.478GlyTyr: 3.478 ± 0.572
0.0GlyXaa: 0.0 ± 0.0
His
0.797HisAla: 0.797 ± 0.241
0.29HisCys: 0.29 ± 0.145
1.232HisAsp: 1.232 ± 0.359
1.304HisGlu: 1.304 ± 0.295
0.942HisPhe: 0.942 ± 0.302
1.304HisGly: 1.304 ± 0.325
0.217HisHis: 0.217 ± 0.131
1.666HisIle: 1.666 ± 0.402
1.232HisLys: 1.232 ± 0.263
0.797HisLeu: 0.797 ± 0.269
0.435HisMet: 0.435 ± 0.205
0.869HisAsn: 0.869 ± 0.234
0.58HisPro: 0.58 ± 0.178
0.507HisGln: 0.507 ± 0.186
1.014HisArg: 1.014 ± 0.295
1.014HisSer: 1.014 ± 0.214
0.652HisThr: 0.652 ± 0.216
0.942HisVal: 0.942 ± 0.265
0.652HisTrp: 0.652 ± 0.203
0.58HisTyr: 0.58 ± 0.16
0.0HisXaa: 0.0 ± 0.0
Ile
3.405IleAla: 3.405 ± 0.589
0.58IleCys: 0.58 ± 0.213
5.434IleAsp: 5.434 ± 0.65
4.782IleGlu: 4.782 ± 0.593
2.029IlePhe: 2.029 ± 0.429
4.637IleGly: 4.637 ± 0.763
1.594IleHis: 1.594 ± 0.391
4.564IleIle: 4.564 ± 0.615
6.738IleLys: 6.738 ± 0.813
4.637IleLeu: 4.637 ± 0.612
1.956IleMet: 1.956 ± 0.425
3.26IleAsn: 3.26 ± 0.445
2.246IlePro: 2.246 ± 0.512
2.825IleGln: 2.825 ± 0.462
2.825IleArg: 2.825 ± 0.378
4.419IleSer: 4.419 ± 0.542
3.622IleThr: 3.622 ± 0.492
5.071IleVal: 5.071 ± 0.51
0.797IleTrp: 0.797 ± 0.258
2.753IleTyr: 2.753 ± 0.521
0.0IleXaa: 0.0 ± 0.0
Lys
6.303LysAla: 6.303 ± 0.761
0.58LysCys: 0.58 ± 0.208
4.999LysAsp: 4.999 ± 0.579
7.607LysGlu: 7.607 ± 0.715
2.536LysPhe: 2.536 ± 0.407
5.216LysGly: 5.216 ± 0.549
1.594LysHis: 1.594 ± 0.375
5.361LysIle: 5.361 ± 0.569
8.259LysLys: 8.259 ± 1.023
6.375LysLeu: 6.375 ± 0.839
3.478LysMet: 3.478 ± 0.555
5.941LysAsn: 5.941 ± 0.768
2.753LysPro: 2.753 ± 0.365
2.463LysGln: 2.463 ± 0.44
5.289LysArg: 5.289 ± 0.815
4.926LysSer: 4.926 ± 0.973
5.506LysThr: 5.506 ± 0.59
5.434LysVal: 5.434 ± 0.639
1.014LysTrp: 1.014 ± 0.265
2.898LysTyr: 2.898 ± 0.439
0.0LysXaa: 0.0 ± 0.0
Leu
5.216LeuAla: 5.216 ± 0.484
0.58LeuCys: 0.58 ± 0.225
3.695LeuAsp: 3.695 ± 0.427
6.375LeuGlu: 6.375 ± 0.636
2.753LeuPhe: 2.753 ± 0.373
4.709LeuGly: 4.709 ± 0.638
1.521LeuHis: 1.521 ± 0.248
4.709LeuIle: 4.709 ± 0.583
7.317LeuLys: 7.317 ± 0.731
3.84LeuLeu: 3.84 ± 0.534
1.811LeuMet: 1.811 ± 0.378
3.405LeuAsn: 3.405 ± 0.379
2.463LeuPro: 2.463 ± 0.502
2.825LeuGln: 2.825 ± 0.559
4.709LeuArg: 4.709 ± 0.621
4.564LeuSer: 4.564 ± 0.503
4.854LeuThr: 4.854 ± 0.441
3.333LeuVal: 3.333 ± 0.431
0.797LeuTrp: 0.797 ± 0.24
1.811LeuTyr: 1.811 ± 0.37
0.0LeuXaa: 0.0 ± 0.0
Met
2.753MetAla: 2.753 ± 0.683
0.362MetCys: 0.362 ± 0.171
1.666MetAsp: 1.666 ± 0.332
2.463MetGlu: 2.463 ± 0.566
0.507MetPhe: 0.507 ± 0.173
1.956MetGly: 1.956 ± 0.348
0.362MetHis: 0.362 ± 0.141
1.884MetIle: 1.884 ± 0.351
3.912MetLys: 3.912 ± 0.45
1.087MetLeu: 1.087 ± 0.243
1.739MetMet: 1.739 ± 0.384
2.681MetAsn: 2.681 ± 0.39
0.797MetPro: 0.797 ± 0.223
1.159MetGln: 1.159 ± 0.325
1.594MetArg: 1.594 ± 0.343
2.608MetSer: 2.608 ± 0.365
1.956MetThr: 1.956 ± 0.32
1.159MetVal: 1.159 ± 0.275
0.652MetTrp: 0.652 ± 0.236
0.58MetTyr: 0.58 ± 0.248
0.0MetXaa: 0.0 ± 0.0
Asn
4.564AsnAla: 4.564 ± 0.815
0.362AsnCys: 0.362 ± 0.151
3.26AsnAsp: 3.26 ± 0.52
4.202AsnGlu: 4.202 ± 0.692
1.811AsnPhe: 1.811 ± 0.432
4.419AsnGly: 4.419 ± 0.886
1.377AsnHis: 1.377 ± 0.292
2.97AsnIle: 2.97 ± 0.455
4.347AsnLys: 4.347 ± 0.572
3.55AsnLeu: 3.55 ± 0.438
2.029AsnMet: 2.029 ± 0.517
2.608AsnAsn: 2.608 ± 0.639
2.101AsnPro: 2.101 ± 0.458
1.014AsnGln: 1.014 ± 0.296
2.536AsnArg: 2.536 ± 0.393
2.173AsnSer: 2.173 ± 0.364
3.043AsnThr: 3.043 ± 0.503
3.043AsnVal: 3.043 ± 0.491
0.942AsnTrp: 0.942 ± 0.199
1.666AsnTyr: 1.666 ± 0.31
0.0AsnXaa: 0.0 ± 0.0
Pro
2.318ProAla: 2.318 ± 0.389
0.072ProCys: 0.072 ± 0.074
1.739ProAsp: 1.739 ± 0.32
2.173ProGlu: 2.173 ± 0.394
1.377ProPhe: 1.377 ± 0.286
2.318ProGly: 2.318 ± 0.488
0.724ProHis: 0.724 ± 0.218
1.594ProIle: 1.594 ± 0.284
2.681ProLys: 2.681 ± 0.383
1.956ProLeu: 1.956 ± 0.415
0.58ProMet: 0.58 ± 0.177
1.014ProAsn: 1.014 ± 0.227
1.159ProPro: 1.159 ± 0.297
1.159ProGln: 1.159 ± 0.272
1.304ProArg: 1.304 ± 0.316
1.521ProSer: 1.521 ± 0.264
1.232ProThr: 1.232 ± 0.324
2.753ProVal: 2.753 ± 0.352
0.145ProTrp: 0.145 ± 0.087
1.521ProTyr: 1.521 ± 0.363
0.0ProXaa: 0.0 ± 0.0
Gln
1.811GlnAla: 1.811 ± 0.385
0.217GlnCys: 0.217 ± 0.117
1.811GlnAsp: 1.811 ± 0.339
3.55GlnGlu: 3.55 ± 0.55
0.942GlnPhe: 0.942 ± 0.229
2.246GlnGly: 2.246 ± 0.372
0.435GlnHis: 0.435 ± 0.157
2.029GlnIle: 2.029 ± 0.37
2.97GlnLys: 2.97 ± 0.47
2.753GlnLeu: 2.753 ± 0.393
1.159GlnMet: 1.159 ± 0.335
1.449GlnAsn: 1.449 ± 0.356
0.797GlnPro: 0.797 ± 0.239
1.521GlnGln: 1.521 ± 0.299
1.521GlnArg: 1.521 ± 0.33
1.956GlnSer: 1.956 ± 0.511
1.811GlnThr: 1.811 ± 0.36
2.101GlnVal: 2.101 ± 0.399
0.29GlnTrp: 0.29 ± 0.148
1.159GlnTyr: 1.159 ± 0.319
0.0GlnXaa: 0.0 ± 0.0
Arg
2.536ArgAla: 2.536 ± 0.415
0.652ArgCys: 0.652 ± 0.199
3.26ArgAsp: 3.26 ± 0.487
3.55ArgGlu: 3.55 ± 0.497
2.898ArgPhe: 2.898 ± 0.476
3.767ArgGly: 3.767 ± 0.509
0.869ArgHis: 0.869 ± 0.213
4.057ArgIle: 4.057 ± 0.634
3.55ArgLys: 3.55 ± 0.638
4.492ArgLeu: 4.492 ± 0.564
1.594ArgMet: 1.594 ± 0.421
2.391ArgAsn: 2.391 ± 0.431
1.232ArgPro: 1.232 ± 0.338
2.029ArgGln: 2.029 ± 0.47
3.043ArgArg: 3.043 ± 0.519
1.956ArgSer: 1.956 ± 0.362
4.057ArgThr: 4.057 ± 0.596
3.043ArgVal: 3.043 ± 0.439
0.724ArgTrp: 0.724 ± 0.178
1.666ArgTyr: 1.666 ± 0.362
0.0ArgXaa: 0.0 ± 0.0
Ser
3.695SerAla: 3.695 ± 0.853
0.362SerCys: 0.362 ± 0.144
3.84SerAsp: 3.84 ± 0.494
4.274SerGlu: 4.274 ± 0.586
3.115SerPhe: 3.115 ± 0.536
5.289SerGly: 5.289 ± 0.477
0.724SerHis: 0.724 ± 0.2
3.767SerIle: 3.767 ± 0.528
4.347SerLys: 4.347 ± 0.451
4.202SerLeu: 4.202 ± 0.688
2.101SerMet: 2.101 ± 0.419
1.666SerAsn: 1.666 ± 0.335
1.521SerPro: 1.521 ± 0.327
1.594SerGln: 1.594 ± 0.309
2.97SerArg: 2.97 ± 0.435
3.333SerSer: 3.333 ± 0.779
3.405SerThr: 3.405 ± 0.461
3.55SerVal: 3.55 ± 0.623
1.014SerTrp: 1.014 ± 0.267
2.101SerTyr: 2.101 ± 0.445
0.0SerXaa: 0.0 ± 0.0
Thr
5.071ThrAla: 5.071 ± 0.843
0.29ThrCys: 0.29 ± 0.136
3.84ThrAsp: 3.84 ± 0.508
3.333ThrGlu: 3.333 ± 0.575
2.173ThrPhe: 2.173 ± 0.359
4.564ThrGly: 4.564 ± 0.65
0.942ThrHis: 0.942 ± 0.226
4.13ThrIle: 4.13 ± 0.55
4.854ThrLys: 4.854 ± 0.706
3.333ThrLeu: 3.333 ± 0.505
1.521ThrMet: 1.521 ± 0.32
3.115ThrAsn: 3.115 ± 0.477
2.536ThrPro: 2.536 ± 0.41
1.594ThrGln: 1.594 ± 0.39
2.681ThrArg: 2.681 ± 0.357
3.26ThrSer: 3.26 ± 0.789
3.478ThrThr: 3.478 ± 0.511
4.564ThrVal: 4.564 ± 0.794
0.942ThrTrp: 0.942 ± 0.264
2.101ThrTyr: 2.101 ± 0.454
0.0ThrXaa: 0.0 ± 0.0
Val
4.347ValAla: 4.347 ± 0.548
0.217ValCys: 0.217 ± 0.122
3.84ValAsp: 3.84 ± 0.567
5.651ValGlu: 5.651 ± 0.674
2.391ValPhe: 2.391 ± 0.43
3.333ValGly: 3.333 ± 0.557
0.724ValHis: 0.724 ± 0.205
5.216ValIle: 5.216 ± 0.582
5.578ValLys: 5.578 ± 0.564
4.854ValLeu: 4.854 ± 0.569
1.666ValMet: 1.666 ± 0.42
3.115ValAsn: 3.115 ± 0.473
1.811ValPro: 1.811 ± 0.29
2.318ValGln: 2.318 ± 0.348
2.898ValArg: 2.898 ± 0.436
4.492ValSer: 4.492 ± 0.455
3.478ValThr: 3.478 ± 0.514
3.695ValVal: 3.695 ± 0.533
1.449ValTrp: 1.449 ± 0.668
2.753ValTyr: 2.753 ± 0.447
0.0ValXaa: 0.0 ± 0.0
Trp
0.58TrpAla: 0.58 ± 0.18
0.145TrpCys: 0.145 ± 0.106
0.797TrpAsp: 0.797 ± 0.308
0.58TrpGlu: 0.58 ± 0.193
0.507TrpPhe: 0.507 ± 0.184
1.304TrpGly: 1.304 ± 0.231
0.29TrpHis: 0.29 ± 0.13
1.449TrpIle: 1.449 ± 0.332
1.014TrpLys: 1.014 ± 0.277
0.724TrpLeu: 0.724 ± 0.188
0.435TrpMet: 0.435 ± 0.174
1.449TrpAsn: 1.449 ± 0.618
0.29TrpPro: 0.29 ± 0.161
0.507TrpGln: 0.507 ± 0.167
0.797TrpArg: 0.797 ± 0.231
1.159TrpSer: 1.159 ± 0.319
0.942TrpThr: 0.942 ± 0.326
0.724TrpVal: 0.724 ± 0.251
0.145TrpTrp: 0.145 ± 0.103
0.29TrpTyr: 0.29 ± 0.155
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.536TyrAla: 2.536 ± 0.385
0.362TyrCys: 0.362 ± 0.156
2.029TyrAsp: 2.029 ± 0.323
2.825TyrGlu: 2.825 ± 0.477
1.232TyrPhe: 1.232 ± 0.33
3.188TyrGly: 3.188 ± 0.597
0.869TyrHis: 0.869 ± 0.252
2.246TyrIle: 2.246 ± 0.37
2.898TyrLys: 2.898 ± 0.513
3.043TyrLeu: 3.043 ± 0.488
0.724TyrMet: 0.724 ± 0.176
2.246TyrAsn: 2.246 ± 0.429
0.869TyrPro: 0.869 ± 0.245
0.652TyrGln: 0.652 ± 0.176
1.449TyrArg: 1.449 ± 0.333
1.666TyrSer: 1.666 ± 0.294
2.173TyrThr: 2.173 ± 0.44
2.246TyrVal: 2.246 ± 0.439
0.724TyrTrp: 0.724 ± 0.249
1.811TyrTyr: 1.811 ± 0.404
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (13804 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski