Amino acid dipepetide frequency for Escherichia phage vB_EcoP_PHB19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.301AlaAla: 9.301 ± 1.391
0.576AlaCys: 0.576 ± 0.193
5.679AlaAsp: 5.679 ± 0.706
5.433AlaGlu: 5.433 ± 0.567
2.881AlaPhe: 2.881 ± 0.45
6.667AlaGly: 6.667 ± 0.89
1.811AlaHis: 1.811 ± 0.289
4.939AlaIle: 4.939 ± 0.916
7.243AlaLys: 7.243 ± 0.666
8.725AlaLeu: 8.725 ± 1.104
2.963AlaMet: 2.963 ± 0.602
4.198AlaAsn: 4.198 ± 0.53
2.387AlaPro: 2.387 ± 0.332
3.869AlaGln: 3.869 ± 0.698
5.515AlaArg: 5.515 ± 0.597
5.679AlaSer: 5.679 ± 0.88
3.292AlaThr: 3.292 ± 0.524
5.103AlaVal: 5.103 ± 0.576
1.564AlaTrp: 1.564 ± 0.398
2.634AlaTyr: 2.634 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
0.741CysAla: 0.741 ± 0.291
0.165CysCys: 0.165 ± 0.118
1.317CysAsp: 1.317 ± 0.492
0.247CysGlu: 0.247 ± 0.165
0.741CysPhe: 0.741 ± 0.26
0.741CysGly: 0.741 ± 0.33
0.576CysHis: 0.576 ± 0.271
0.741CysIle: 0.741 ± 0.278
0.412CysLys: 0.412 ± 0.206
0.741CysLeu: 0.741 ± 0.273
0.165CysMet: 0.165 ± 0.138
0.247CysAsn: 0.247 ± 0.148
0.494CysPro: 0.494 ± 0.213
0.412CysGln: 0.412 ± 0.198
0.576CysArg: 0.576 ± 0.245
0.988CysSer: 0.988 ± 0.271
0.247CysThr: 0.247 ± 0.166
1.07CysVal: 1.07 ± 0.367
0.412CysTrp: 0.412 ± 0.232
0.165CysTyr: 0.165 ± 0.104
0.0CysXaa: 0.0 ± 0.0
Asp
5.268AspAla: 5.268 ± 0.653
0.576AspCys: 0.576 ± 0.232
4.033AspAsp: 4.033 ± 0.595
4.28AspGlu: 4.28 ± 0.559
3.128AspPhe: 3.128 ± 0.339
6.256AspGly: 6.256 ± 0.777
0.823AspHis: 0.823 ± 0.259
3.21AspIle: 3.21 ± 0.383
4.033AspLys: 4.033 ± 0.703
4.445AspLeu: 4.445 ± 0.761
2.305AspMet: 2.305 ± 0.501
3.128AspAsn: 3.128 ± 0.418
2.963AspPro: 2.963 ± 0.603
1.564AspGln: 1.564 ± 0.414
2.305AspArg: 2.305 ± 0.426
3.539AspSer: 3.539 ± 0.595
4.28AspThr: 4.28 ± 0.578
5.103AspVal: 5.103 ± 0.539
0.905AspTrp: 0.905 ± 0.348
1.893AspTyr: 1.893 ± 0.392
0.0AspXaa: 0.0 ± 0.0
Glu
7.161GluAla: 7.161 ± 0.936
0.576GluCys: 0.576 ± 0.2
4.28GluAsp: 4.28 ± 0.888
6.996GluGlu: 6.996 ± 1.106
2.469GluPhe: 2.469 ± 0.443
4.774GluGly: 4.774 ± 0.773
1.399GluHis: 1.399 ± 0.386
3.375GluIle: 3.375 ± 0.533
2.963GluLys: 2.963 ± 0.537
6.667GluLeu: 6.667 ± 0.618
2.222GluMet: 2.222 ± 0.54
2.881GluAsn: 2.881 ± 0.534
1.482GluPro: 1.482 ± 0.333
3.786GluGln: 3.786 ± 0.975
4.116GluArg: 4.116 ± 0.486
5.268GluSer: 5.268 ± 0.717
3.539GluThr: 3.539 ± 0.583
4.774GluVal: 4.774 ± 0.778
1.235GluTrp: 1.235 ± 0.389
3.128GluTyr: 3.128 ± 0.521
0.0GluXaa: 0.0 ± 0.0
Phe
1.811PheAla: 1.811 ± 0.336
0.412PheCys: 0.412 ± 0.189
2.881PheAsp: 2.881 ± 0.449
3.21PheGlu: 3.21 ± 0.549
0.988PhePhe: 0.988 ± 0.268
3.375PheGly: 3.375 ± 0.509
0.494PheHis: 0.494 ± 0.256
1.811PheIle: 1.811 ± 0.389
2.799PheLys: 2.799 ± 0.451
2.716PheLeu: 2.716 ± 0.397
1.482PheMet: 1.482 ± 0.25
2.058PheAsn: 2.058 ± 0.5
1.399PhePro: 1.399 ± 0.404
1.07PheGln: 1.07 ± 0.281
1.646PheArg: 1.646 ± 0.295
2.14PheSer: 2.14 ± 0.457
2.552PheThr: 2.552 ± 0.367
1.893PheVal: 1.893 ± 0.495
0.247PheTrp: 0.247 ± 0.12
0.905PheTyr: 0.905 ± 0.287
0.0PheXaa: 0.0 ± 0.0
Gly
6.585GlyAla: 6.585 ± 0.899
1.152GlyCys: 1.152 ± 0.363
4.28GlyAsp: 4.28 ± 0.508
5.021GlyGlu: 5.021 ± 0.582
3.292GlyPhe: 3.292 ± 0.507
5.597GlyGly: 5.597 ± 0.923
1.399GlyHis: 1.399 ± 0.304
4.609GlyIle: 4.609 ± 0.703
4.939GlyLys: 4.939 ± 0.768
6.338GlyLeu: 6.338 ± 0.872
2.058GlyMet: 2.058 ± 0.501
2.881GlyAsn: 2.881 ± 0.56
0.741GlyPro: 0.741 ± 0.257
2.881GlyGln: 2.881 ± 0.435
4.362GlyArg: 4.362 ± 0.427
4.939GlySer: 4.939 ± 0.695
3.128GlyThr: 3.128 ± 0.407
4.692GlyVal: 4.692 ± 0.579
1.811GlyTrp: 1.811 ± 0.514
2.799GlyTyr: 2.799 ± 0.52
0.0GlyXaa: 0.0 ± 0.0
His
1.152HisAla: 1.152 ± 0.372
0.329HisCys: 0.329 ± 0.15
1.152HisAsp: 1.152 ± 0.25
1.811HisGlu: 1.811 ± 0.546
0.658HisPhe: 0.658 ± 0.282
1.152HisGly: 1.152 ± 0.283
0.494HisHis: 0.494 ± 0.167
1.646HisIle: 1.646 ± 0.453
1.235HisLys: 1.235 ± 0.321
1.729HisLeu: 1.729 ± 0.442
0.494HisMet: 0.494 ± 0.175
0.576HisAsn: 0.576 ± 0.219
0.412HisPro: 0.412 ± 0.177
0.165HisGln: 0.165 ± 0.174
0.988HisArg: 0.988 ± 0.288
1.235HisSer: 1.235 ± 0.254
0.412HisThr: 0.412 ± 0.18
1.317HisVal: 1.317 ± 0.326
0.247HisTrp: 0.247 ± 0.139
0.741HisTyr: 0.741 ± 0.177
0.0HisXaa: 0.0 ± 0.0
Ile
4.033IleAla: 4.033 ± 0.513
0.494IleCys: 0.494 ± 0.189
4.445IleAsp: 4.445 ± 0.679
3.457IleGlu: 3.457 ± 0.635
0.823IlePhe: 0.823 ± 0.194
4.28IleGly: 4.28 ± 0.471
1.235IleHis: 1.235 ± 0.413
3.292IleIle: 3.292 ± 0.721
4.116IleLys: 4.116 ± 0.526
3.786IleLeu: 3.786 ± 0.551
1.564IleMet: 1.564 ± 0.272
3.21IleAsn: 3.21 ± 0.574
2.799IlePro: 2.799 ± 0.44
1.564IleGln: 1.564 ± 0.461
3.292IleArg: 3.292 ± 0.606
3.046IleSer: 3.046 ± 0.492
2.963IleThr: 2.963 ± 0.477
3.21IleVal: 3.21 ± 0.456
0.576IleTrp: 0.576 ± 0.198
1.729IleTyr: 1.729 ± 0.285
0.0IleXaa: 0.0 ± 0.0
Lys
7.984LysAla: 7.984 ± 1.14
1.07LysCys: 1.07 ± 0.478
3.292LysAsp: 3.292 ± 0.416
5.268LysGlu: 5.268 ± 0.768
2.14LysPhe: 2.14 ± 0.291
4.362LysGly: 4.362 ± 0.509
1.482LysHis: 1.482 ± 0.321
2.387LysIle: 2.387 ± 0.47
4.362LysLys: 4.362 ± 0.928
5.268LysLeu: 5.268 ± 0.797
2.387LysMet: 2.387 ± 0.524
2.552LysAsn: 2.552 ± 0.327
2.552LysPro: 2.552 ± 0.528
2.305LysGln: 2.305 ± 0.545
4.198LysArg: 4.198 ± 0.7
3.951LysSer: 3.951 ± 0.636
2.552LysThr: 2.552 ± 0.392
5.021LysVal: 5.021 ± 0.854
0.823LysTrp: 0.823 ± 0.209
2.799LysTyr: 2.799 ± 0.426
0.0LysXaa: 0.0 ± 0.0
Leu
8.725LeuAla: 8.725 ± 1.012
0.329LeuCys: 0.329 ± 0.177
4.609LeuAsp: 4.609 ± 0.589
6.173LeuGlu: 6.173 ± 0.78
2.222LeuPhe: 2.222 ± 0.377
4.28LeuGly: 4.28 ± 0.728
1.152LeuHis: 1.152 ± 0.277
3.951LeuIle: 3.951 ± 0.563
5.926LeuLys: 5.926 ± 0.759
5.103LeuLeu: 5.103 ± 0.798
2.222LeuMet: 2.222 ± 0.449
3.869LeuAsn: 3.869 ± 0.497
3.046LeuPro: 3.046 ± 0.666
4.033LeuGln: 4.033 ± 0.692
6.667LeuArg: 6.667 ± 0.803
4.939LeuSer: 4.939 ± 0.519
5.103LeuThr: 5.103 ± 0.734
4.116LeuVal: 4.116 ± 0.502
1.317LeuTrp: 1.317 ± 0.36
2.552LeuTyr: 2.552 ± 0.444
0.0LeuXaa: 0.0 ± 0.0
Met
3.128MetAla: 3.128 ± 0.486
0.247MetCys: 0.247 ± 0.174
1.893MetAsp: 1.893 ± 0.45
1.729MetGlu: 1.729 ± 0.366
1.152MetPhe: 1.152 ± 0.324
2.058MetGly: 2.058 ± 0.427
0.412MetHis: 0.412 ± 0.158
1.235MetIle: 1.235 ± 0.286
1.482MetLys: 1.482 ± 0.317
3.128MetLeu: 3.128 ± 0.618
0.823MetMet: 0.823 ± 0.312
1.235MetAsn: 1.235 ± 0.343
1.482MetPro: 1.482 ± 0.268
1.07MetGln: 1.07 ± 0.28
1.482MetArg: 1.482 ± 0.33
2.14MetSer: 2.14 ± 0.468
1.975MetThr: 1.975 ± 0.381
2.305MetVal: 2.305 ± 0.458
0.165MetTrp: 0.165 ± 0.136
0.823MetTyr: 0.823 ± 0.25
0.0MetXaa: 0.0 ± 0.0
Asn
4.198AsnAla: 4.198 ± 0.727
0.988AsnCys: 0.988 ± 0.421
2.14AsnAsp: 2.14 ± 0.378
2.799AsnGlu: 2.799 ± 0.399
1.729AsnPhe: 1.729 ± 0.408
4.362AsnGly: 4.362 ± 0.658
0.576AsnHis: 0.576 ± 0.189
2.469AsnIle: 2.469 ± 0.357
2.469AsnLys: 2.469 ± 0.426
3.375AsnLeu: 3.375 ± 0.601
1.235AsnMet: 1.235 ± 0.271
1.811AsnAsn: 1.811 ± 0.46
2.799AsnPro: 2.799 ± 0.45
1.399AsnGln: 1.399 ± 0.317
2.387AsnArg: 2.387 ± 0.523
3.128AsnSer: 3.128 ± 0.951
2.305AsnThr: 2.305 ± 0.515
3.21AsnVal: 3.21 ± 0.628
0.658AsnTrp: 0.658 ± 0.201
1.564AsnTyr: 1.564 ± 0.381
0.0AsnXaa: 0.0 ± 0.0
Pro
3.046ProAla: 3.046 ± 0.429
0.412ProCys: 0.412 ± 0.178
2.634ProAsp: 2.634 ± 0.558
3.622ProGlu: 3.622 ± 0.742
1.07ProPhe: 1.07 ± 0.252
0.494ProGly: 0.494 ± 0.218
0.576ProHis: 0.576 ± 0.183
1.811ProIle: 1.811 ± 0.357
2.305ProLys: 2.305 ± 0.496
2.552ProLeu: 2.552 ± 0.575
1.317ProMet: 1.317 ± 0.304
2.469ProAsn: 2.469 ± 0.505
0.741ProPro: 0.741 ± 0.226
1.152ProGln: 1.152 ± 0.329
1.482ProArg: 1.482 ± 0.419
2.305ProSer: 2.305 ± 0.389
2.058ProThr: 2.058 ± 0.377
1.893ProVal: 1.893 ± 0.334
0.741ProTrp: 0.741 ± 0.196
1.399ProTyr: 1.399 ± 0.397
0.0ProXaa: 0.0 ± 0.0
Gln
3.622GlnAla: 3.622 ± 0.892
0.247GlnCys: 0.247 ± 0.14
1.646GlnAsp: 1.646 ± 0.307
2.634GlnGlu: 2.634 ± 0.551
1.893GlnPhe: 1.893 ± 0.333
2.305GlnGly: 2.305 ± 0.369
0.494GlnHis: 0.494 ± 0.26
1.564GlnIle: 1.564 ± 0.358
2.387GlnLys: 2.387 ± 0.481
3.951GlnLeu: 3.951 ± 0.481
1.235GlnMet: 1.235 ± 0.304
1.235GlnAsn: 1.235 ± 0.365
1.482GlnPro: 1.482 ± 0.348
1.646GlnGln: 1.646 ± 0.317
2.387GlnArg: 2.387 ± 0.562
1.811GlnSer: 1.811 ± 0.519
1.729GlnThr: 1.729 ± 0.433
2.14GlnVal: 2.14 ± 0.526
1.152GlnTrp: 1.152 ± 0.281
1.07GlnTyr: 1.07 ± 0.337
0.0GlnXaa: 0.0 ± 0.0
Arg
4.774ArgAla: 4.774 ± 0.541
0.988ArgCys: 0.988 ± 0.308
4.28ArgAsp: 4.28 ± 0.579
4.527ArgGlu: 4.527 ± 0.682
2.387ArgPhe: 2.387 ± 0.461
3.951ArgGly: 3.951 ± 0.522
0.823ArgHis: 0.823 ± 0.249
3.375ArgIle: 3.375 ± 0.322
4.198ArgLys: 4.198 ± 0.656
4.692ArgLeu: 4.692 ± 0.582
1.564ArgMet: 1.564 ± 0.408
2.222ArgAsn: 2.222 ± 0.584
1.564ArgPro: 1.564 ± 0.267
1.811ArgGln: 1.811 ± 0.379
2.799ArgArg: 2.799 ± 0.433
3.869ArgSer: 3.869 ± 0.586
2.552ArgThr: 2.552 ± 0.373
3.457ArgVal: 3.457 ± 0.514
0.741ArgTrp: 0.741 ± 0.237
1.729ArgTyr: 1.729 ± 0.386
0.0ArgXaa: 0.0 ± 0.0
Ser
5.186SerAla: 5.186 ± 0.709
0.823SerCys: 0.823 ± 0.295
5.021SerAsp: 5.021 ± 0.444
3.704SerGlu: 3.704 ± 0.545
2.799SerPhe: 2.799 ± 0.446
5.844SerGly: 5.844 ± 0.908
1.399SerHis: 1.399 ± 0.348
3.951SerIle: 3.951 ± 0.628
3.457SerLys: 3.457 ± 0.722
4.445SerLeu: 4.445 ± 0.596
1.564SerMet: 1.564 ± 0.391
2.552SerAsn: 2.552 ± 0.429
1.729SerPro: 1.729 ± 0.393
2.222SerGln: 2.222 ± 0.546
3.046SerArg: 3.046 ± 0.443
3.292SerSer: 3.292 ± 0.669
3.951SerThr: 3.951 ± 0.653
4.28SerVal: 4.28 ± 0.603
0.823SerTrp: 0.823 ± 0.258
2.14SerTyr: 2.14 ± 0.419
0.0SerXaa: 0.0 ± 0.0
Thr
4.527ThrAla: 4.527 ± 0.608
0.658ThrCys: 0.658 ± 0.263
3.622ThrAsp: 3.622 ± 0.862
3.622ThrGlu: 3.622 ± 0.573
1.893ThrPhe: 1.893 ± 0.5
5.433ThrGly: 5.433 ± 0.683
1.235ThrHis: 1.235 ± 0.342
3.539ThrIle: 3.539 ± 0.65
5.021ThrLys: 5.021 ± 0.605
3.869ThrLeu: 3.869 ± 0.46
1.317ThrMet: 1.317 ± 0.354
1.893ThrAsn: 1.893 ± 0.516
2.222ThrPro: 2.222 ± 0.423
1.975ThrGln: 1.975 ± 0.39
2.387ThrArg: 2.387 ± 0.411
3.046ThrSer: 3.046 ± 0.597
3.21ThrThr: 3.21 ± 0.634
3.128ThrVal: 3.128 ± 0.545
0.494ThrTrp: 0.494 ± 0.217
1.482ThrTyr: 1.482 ± 0.513
0.0ThrXaa: 0.0 ± 0.0
Val
5.433ValAla: 5.433 ± 0.81
0.494ValCys: 0.494 ± 0.201
3.539ValAsp: 3.539 ± 0.472
4.609ValGlu: 4.609 ± 0.662
2.387ValPhe: 2.387 ± 0.533
4.445ValGly: 4.445 ± 0.567
0.988ValHis: 0.988 ± 0.28
3.21ValIle: 3.21 ± 0.6
4.28ValLys: 4.28 ± 0.715
4.527ValLeu: 4.527 ± 0.616
1.646ValMet: 1.646 ± 0.34
3.375ValAsn: 3.375 ± 1.077
2.634ValPro: 2.634 ± 0.366
2.058ValGln: 2.058 ± 0.363
3.869ValArg: 3.869 ± 0.507
3.786ValSer: 3.786 ± 0.608
5.433ValThr: 5.433 ± 0.801
4.28ValVal: 4.28 ± 0.614
0.905ValTrp: 0.905 ± 0.262
2.634ValTyr: 2.634 ± 0.536
0.0ValXaa: 0.0 ± 0.0
Trp
0.905TrpAla: 0.905 ± 0.272
0.494TrpCys: 0.494 ± 0.273
0.823TrpAsp: 0.823 ± 0.28
1.317TrpGlu: 1.317 ± 0.35
0.494TrpPhe: 0.494 ± 0.199
0.823TrpGly: 0.823 ± 0.281
0.412TrpHis: 0.412 ± 0.202
0.658TrpIle: 0.658 ± 0.26
1.152TrpLys: 1.152 ± 0.334
1.729TrpLeu: 1.729 ± 0.337
0.247TrpMet: 0.247 ± 0.129
1.07TrpAsn: 1.07 ± 0.31
0.247TrpPro: 0.247 ± 0.131
0.576TrpGln: 0.576 ± 0.207
0.741TrpArg: 0.741 ± 0.278
1.07TrpSer: 1.07 ± 0.344
0.988TrpThr: 0.988 ± 0.262
1.399TrpVal: 1.399 ± 0.453
0.576TrpTrp: 0.576 ± 0.227
0.165TrpTyr: 0.165 ± 0.114
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.881TyrAla: 2.881 ± 0.441
0.165TyrCys: 0.165 ± 0.126
2.469TyrAsp: 2.469 ± 0.526
2.634TyrGlu: 2.634 ± 0.529
0.741TyrPhe: 0.741 ± 0.232
2.305TyrGly: 2.305 ± 0.445
0.165TyrHis: 0.165 ± 0.111
2.14TyrIle: 2.14 ± 0.552
1.975TyrLys: 1.975 ± 0.366
2.552TyrLeu: 2.552 ± 0.502
1.07TyrMet: 1.07 ± 0.274
2.14TyrAsn: 2.14 ± 0.354
0.905TyrPro: 0.905 ± 0.293
1.152TyrGln: 1.152 ± 0.34
2.058TyrArg: 2.058 ± 0.434
2.058TyrSer: 2.058 ± 0.396
2.305TyrThr: 2.305 ± 0.375
2.14TyrVal: 2.14 ± 0.447
0.494TyrTrp: 0.494 ± 0.251
0.741TyrTyr: 0.741 ± 0.277
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (12150 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski