Amino acid dipepetide frequency for Nitratiruptor phage NrS-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.298AlaAla: 6.298 ± 1.598
0.27AlaCys: 0.27 ± 0.147
4.139AlaAsp: 4.139 ± 0.648
3.689AlaGlu: 3.689 ± 0.583
2.699AlaPhe: 2.699 ± 0.412
5.218AlaGly: 5.218 ± 0.91
1.17AlaHis: 1.17 ± 0.415
6.478AlaIle: 6.478 ± 0.801
8.187AlaLys: 8.187 ± 0.935
7.377AlaLeu: 7.377 ± 1.036
1.979AlaMet: 1.979 ± 0.354
4.858AlaAsn: 4.858 ± 0.891
1.439AlaPro: 1.439 ± 0.439
3.149AlaGln: 3.149 ± 0.545
2.339AlaArg: 2.339 ± 0.411
4.498AlaSer: 4.498 ± 0.898
3.239AlaThr: 3.239 ± 0.704
3.779AlaVal: 3.779 ± 0.707
0.36AlaTrp: 0.36 ± 0.227
3.599AlaTyr: 3.599 ± 0.77
0.0AlaXaa: 0.0 ± 0.0
Cys
0.27CysAla: 0.27 ± 0.154
0.09CysCys: 0.09 ± 0.105
0.99CysAsp: 0.99 ± 0.275
0.36CysGlu: 0.36 ± 0.202
0.27CysPhe: 0.27 ± 0.133
0.36CysGly: 0.36 ± 0.185
0.0CysHis: 0.0 ± 0.0
0.63CysIle: 0.63 ± 0.273
1.35CysLys: 1.35 ± 0.469
0.54CysLeu: 0.54 ± 0.22
0.09CysMet: 0.09 ± 0.084
1.08CysAsn: 1.08 ± 0.501
0.54CysPro: 0.54 ± 0.242
0.0CysGln: 0.0 ± 0.0
0.45CysArg: 0.45 ± 0.176
0.27CysSer: 0.27 ± 0.219
0.27CysThr: 0.27 ± 0.163
0.27CysVal: 0.27 ± 0.157
0.09CysTrp: 0.09 ± 0.094
0.54CysTyr: 0.54 ± 0.239
0.0CysXaa: 0.0 ± 0.0
Asp
4.318AspAla: 4.318 ± 0.761
0.54AspCys: 0.54 ± 0.198
3.959AspAsp: 3.959 ± 0.687
5.398AspGlu: 5.398 ± 0.774
2.519AspPhe: 2.519 ± 0.408
4.768AspGly: 4.768 ± 0.838
0.54AspHis: 0.54 ± 0.167
5.578AspIle: 5.578 ± 0.848
6.208AspLys: 6.208 ± 0.938
3.689AspLeu: 3.689 ± 0.509
1.439AspMet: 1.439 ± 0.29
2.969AspAsn: 2.969 ± 0.456
2.429AspPro: 2.429 ± 0.459
1.17AspGln: 1.17 ± 0.339
2.519AspArg: 2.519 ± 0.485
2.789AspSer: 2.789 ± 0.467
3.689AspThr: 3.689 ± 0.526
3.329AspVal: 3.329 ± 0.533
0.81AspTrp: 0.81 ± 0.23
2.699AspTyr: 2.699 ± 0.374
0.0AspXaa: 0.0 ± 0.0
Glu
7.377GluAla: 7.377 ± 0.923
0.72GluCys: 0.72 ± 0.259
3.779GluAsp: 3.779 ± 0.674
6.388GluGlu: 6.388 ± 1.027
3.869GluPhe: 3.869 ± 0.703
3.149GluGly: 3.149 ± 0.483
1.619GluHis: 1.619 ± 0.382
6.298GluIle: 6.298 ± 0.746
6.028GluLys: 6.028 ± 0.76
8.097GluLeu: 8.097 ± 1.347
1.979GluMet: 1.979 ± 0.455
2.879GluAsn: 2.879 ± 0.521
1.529GluPro: 1.529 ± 0.419
2.249GluGln: 2.249 ± 0.463
4.049GluArg: 4.049 ± 0.597
3.689GluSer: 3.689 ± 0.571
3.869GluThr: 3.869 ± 0.667
5.488GluVal: 5.488 ± 0.571
1.439GluTrp: 1.439 ± 0.373
3.959GluTyr: 3.959 ± 0.672
0.0GluXaa: 0.0 ± 0.0
Phe
3.779PheAla: 3.779 ± 0.609
0.54PheCys: 0.54 ± 0.203
3.419PheAsp: 3.419 ± 0.505
4.498PheGlu: 4.498 ± 0.533
1.439PhePhe: 1.439 ± 0.354
2.339PheGly: 2.339 ± 0.503
0.36PheHis: 0.36 ± 0.323
2.069PheIle: 2.069 ± 0.452
3.779PheLys: 3.779 ± 0.571
2.789PheLeu: 2.789 ± 0.619
0.63PheMet: 0.63 ± 0.218
2.429PheAsn: 2.429 ± 0.524
0.99PhePro: 0.99 ± 0.245
1.35PheGln: 1.35 ± 0.381
1.529PheArg: 1.529 ± 0.462
3.149PheSer: 3.149 ± 0.692
2.339PheThr: 2.339 ± 0.478
3.419PheVal: 3.419 ± 0.528
0.63PheTrp: 0.63 ± 0.276
1.439PheTyr: 1.439 ± 0.436
0.0PheXaa: 0.0 ± 0.0
Gly
3.779GlyAla: 3.779 ± 0.684
0.54GlyCys: 0.54 ± 0.215
3.959GlyAsp: 3.959 ± 0.723
5.038GlyGlu: 5.038 ± 0.661
2.249GlyPhe: 2.249 ± 0.446
3.599GlyGly: 3.599 ± 0.762
0.63GlyHis: 0.63 ± 0.234
4.139GlyIle: 4.139 ± 0.78
4.408GlyLys: 4.408 ± 0.709
5.038GlyLeu: 5.038 ± 0.603
1.439GlyMet: 1.439 ± 0.372
2.249GlyAsn: 2.249 ± 0.51
0.72GlyPro: 0.72 ± 0.197
2.069GlyGln: 2.069 ± 0.382
1.979GlyArg: 1.979 ± 0.466
3.959GlySer: 3.959 ± 0.796
3.419GlyThr: 3.419 ± 0.525
3.779GlyVal: 3.779 ± 0.496
0.9GlyTrp: 0.9 ± 0.339
3.779GlyTyr: 3.779 ± 0.614
0.0GlyXaa: 0.0 ± 0.0
His
0.81HisAla: 0.81 ± 0.37
0.36HisCys: 0.36 ± 0.18
0.18HisAsp: 0.18 ± 0.133
0.45HisGlu: 0.45 ± 0.185
1.17HisPhe: 1.17 ± 0.39
0.45HisGly: 0.45 ± 0.206
0.18HisHis: 0.18 ± 0.114
1.709HisIle: 1.709 ± 0.445
1.709HisLys: 1.709 ± 0.418
1.35HisLeu: 1.35 ± 0.389
0.45HisMet: 0.45 ± 0.212
0.9HisAsn: 0.9 ± 0.305
1.529HisPro: 1.529 ± 0.307
0.54HisGln: 0.54 ± 0.272
0.99HisArg: 0.99 ± 0.319
0.36HisSer: 0.36 ± 0.155
0.63HisThr: 0.63 ± 0.222
0.72HisVal: 0.72 ± 0.254
0.27HisTrp: 0.27 ± 0.127
0.81HisTyr: 0.81 ± 0.262
0.0HisXaa: 0.0 ± 0.0
Ile
4.588IleAla: 4.588 ± 0.819
0.63IleCys: 0.63 ± 0.212
6.118IleAsp: 6.118 ± 0.726
8.007IleGlu: 8.007 ± 1.061
3.149IlePhe: 3.149 ± 0.464
4.588IleGly: 4.588 ± 0.659
0.81IleHis: 0.81 ± 0.253
3.509IleIle: 3.509 ± 0.611
7.467IleLys: 7.467 ± 1.018
4.588IleLeu: 4.588 ± 0.574
1.26IleMet: 1.26 ± 0.285
2.519IleAsn: 2.519 ± 0.504
2.249IlePro: 2.249 ± 0.539
3.329IleGln: 3.329 ± 0.49
3.059IleArg: 3.059 ± 0.534
3.959IleSer: 3.959 ± 0.733
4.948IleThr: 4.948 ± 0.703
5.488IleVal: 5.488 ± 0.602
1.35IleTrp: 1.35 ± 0.312
2.429IleTyr: 2.429 ± 0.457
0.0IleXaa: 0.0 ± 0.0
Lys
7.287LysAla: 7.287 ± 0.965
0.72LysCys: 0.72 ± 0.426
5.218LysAsp: 5.218 ± 0.715
10.166LysGlu: 10.166 ± 1.171
3.149LysPhe: 3.149 ± 0.486
3.599LysGly: 3.599 ± 0.566
2.249LysHis: 2.249 ± 0.387
7.557LysIle: 7.557 ± 0.848
8.727LysLys: 8.727 ± 1.21
7.287LysLeu: 7.287 ± 0.864
1.889LysMet: 1.889 ± 0.363
5.128LysAsn: 5.128 ± 0.602
2.699LysPro: 2.699 ± 0.581
2.699LysGln: 2.699 ± 0.467
4.588LysArg: 4.588 ± 0.791
6.838LysSer: 6.838 ± 0.799
6.028LysThr: 6.028 ± 0.861
5.488LysVal: 5.488 ± 0.614
1.26LysTrp: 1.26 ± 0.263
3.779LysTyr: 3.779 ± 0.695
0.0LysXaa: 0.0 ± 0.0
Leu
5.128LeuAla: 5.128 ± 0.7
1.35LeuCys: 1.35 ± 0.467
5.938LeuAsp: 5.938 ± 0.649
6.568LeuGlu: 6.568 ± 0.87
3.329LeuPhe: 3.329 ± 0.506
4.768LeuGly: 4.768 ± 0.856
1.889LeuHis: 1.889 ± 0.373
3.869LeuIle: 3.869 ± 0.512
8.817LeuLys: 8.817 ± 1.274
6.838LeuLeu: 6.838 ± 0.879
1.979LeuMet: 1.979 ± 0.423
3.149LeuAsn: 3.149 ± 0.451
2.249LeuPro: 2.249 ± 0.272
3.509LeuGln: 3.509 ± 0.574
3.689LeuArg: 3.689 ± 0.678
6.028LeuSer: 6.028 ± 0.874
4.498LeuThr: 4.498 ± 0.507
4.049LeuVal: 4.049 ± 0.5
0.72LeuTrp: 0.72 ± 0.272
3.239LeuTyr: 3.239 ± 0.533
0.0LeuXaa: 0.0 ± 0.0
Met
2.609MetAla: 2.609 ± 0.502
0.18MetCys: 0.18 ± 0.119
1.17MetAsp: 1.17 ± 0.287
1.799MetGlu: 1.799 ± 0.486
0.54MetPhe: 0.54 ± 0.255
0.81MetGly: 0.81 ± 0.279
0.27MetHis: 0.27 ± 0.184
1.439MetIle: 1.439 ± 0.341
2.159MetLys: 2.159 ± 0.572
1.35MetLeu: 1.35 ± 0.292
0.36MetMet: 0.36 ± 0.188
1.17MetAsn: 1.17 ± 0.328
0.81MetPro: 0.81 ± 0.249
0.54MetGln: 0.54 ± 0.24
0.99MetArg: 0.99 ± 0.368
1.799MetSer: 1.799 ± 0.4
1.26MetThr: 1.26 ± 0.308
1.17MetVal: 1.17 ± 0.313
0.09MetTrp: 0.09 ± 0.089
0.54MetTyr: 0.54 ± 0.266
0.0MetXaa: 0.0 ± 0.0
Asn
4.408AsnAla: 4.408 ± 1.137
0.36AsnCys: 0.36 ± 0.165
2.519AsnAsp: 2.519 ± 0.554
3.329AsnGlu: 3.329 ± 0.619
2.249AsnPhe: 2.249 ± 0.526
3.239AsnGly: 3.239 ± 0.579
0.63AsnHis: 0.63 ± 0.325
4.588AsnIle: 4.588 ± 0.589
3.149AsnLys: 3.149 ± 0.522
3.419AsnLeu: 3.419 ± 0.504
1.08AsnMet: 1.08 ± 0.357
2.609AsnAsn: 2.609 ± 0.591
2.789AsnPro: 2.789 ± 0.555
1.439AsnGln: 1.439 ± 0.59
2.069AsnArg: 2.069 ± 0.54
3.329AsnSer: 3.329 ± 0.585
2.699AsnThr: 2.699 ± 0.708
2.879AsnVal: 2.879 ± 0.68
0.54AsnTrp: 0.54 ± 0.198
1.709AsnTyr: 1.709 ± 0.374
0.0AsnXaa: 0.0 ± 0.0
Pro
1.619ProAla: 1.619 ± 0.357
0.0ProCys: 0.0 ± 0.0
1.889ProAsp: 1.889 ± 0.32
1.799ProGlu: 1.799 ± 0.369
2.159ProPhe: 2.159 ± 0.355
1.26ProGly: 1.26 ± 0.317
0.45ProHis: 0.45 ± 0.169
2.339ProIle: 2.339 ± 0.457
3.239ProLys: 3.239 ± 0.79
2.249ProLeu: 2.249 ± 0.452
0.27ProMet: 0.27 ± 0.156
1.889ProAsn: 1.889 ± 0.434
0.72ProPro: 0.72 ± 0.219
1.08ProGln: 1.08 ± 0.328
0.81ProArg: 0.81 ± 0.295
1.979ProSer: 1.979 ± 0.449
2.519ProThr: 2.519 ± 0.345
1.979ProVal: 1.979 ± 0.437
0.09ProTrp: 0.09 ± 0.091
1.709ProTyr: 1.709 ± 0.5
0.0ProXaa: 0.0 ± 0.0
Gln
1.709GlnAla: 1.709 ± 0.451
0.18GlnCys: 0.18 ± 0.203
1.26GlnAsp: 1.26 ± 0.392
2.429GlnGlu: 2.429 ± 0.48
1.35GlnPhe: 1.35 ± 0.38
1.709GlnGly: 1.709 ± 0.384
0.63GlnHis: 0.63 ± 0.26
3.329GlnIle: 3.329 ± 0.613
4.408GlnLys: 4.408 ± 0.821
3.779GlnLeu: 3.779 ± 0.65
0.63GlnMet: 0.63 ± 0.206
1.709GlnAsn: 1.709 ± 0.508
0.72GlnPro: 0.72 ± 0.301
0.9GlnGln: 0.9 ± 0.253
1.35GlnArg: 1.35 ± 0.354
2.159GlnSer: 2.159 ± 0.375
1.979GlnThr: 1.979 ± 0.414
2.159GlnVal: 2.159 ± 0.35
0.99GlnTrp: 0.99 ± 0.329
0.9GlnTyr: 0.9 ± 0.263
0.0GlnXaa: 0.0 ± 0.0
Arg
3.329ArgAla: 3.329 ± 0.5
0.54ArgCys: 0.54 ± 0.241
2.519ArgAsp: 2.519 ± 0.458
2.339ArgGlu: 2.339 ± 0.432
2.159ArgPhe: 2.159 ± 0.381
2.249ArgGly: 2.249 ± 0.538
0.9ArgHis: 0.9 ± 0.278
3.149ArgIle: 3.149 ± 0.541
3.959ArgLys: 3.959 ± 0.797
3.779ArgLeu: 3.779 ± 0.513
0.72ArgMet: 0.72 ± 0.327
1.889ArgAsn: 1.889 ± 0.438
1.439ArgPro: 1.439 ± 0.291
1.08ArgGln: 1.08 ± 0.379
1.799ArgArg: 1.799 ± 0.456
1.529ArgSer: 1.529 ± 0.388
1.35ArgThr: 1.35 ± 0.331
1.709ArgVal: 1.709 ± 0.322
1.08ArgTrp: 1.08 ± 0.272
2.609ArgTyr: 2.609 ± 0.409
0.0ArgXaa: 0.0 ± 0.0
Ser
4.588SerAla: 4.588 ± 0.589
0.63SerCys: 0.63 ± 0.296
3.239SerAsp: 3.239 ± 0.624
4.498SerGlu: 4.498 ± 0.828
3.869SerPhe: 3.869 ± 0.589
3.869SerGly: 3.869 ± 0.699
0.99SerHis: 0.99 ± 0.254
4.049SerIle: 4.049 ± 0.526
6.028SerLys: 6.028 ± 0.61
2.879SerLeu: 2.879 ± 0.411
1.619SerMet: 1.619 ± 0.371
3.059SerAsn: 3.059 ± 0.497
1.799SerPro: 1.799 ± 0.379
1.799SerGln: 1.799 ± 0.54
2.339SerArg: 2.339 ± 0.457
3.059SerSer: 3.059 ± 0.644
3.689SerThr: 3.689 ± 0.73
3.869SerVal: 3.869 ± 0.689
1.17SerTrp: 1.17 ± 0.31
2.699SerTyr: 2.699 ± 0.45
0.0SerXaa: 0.0 ± 0.0
Thr
3.869ThrAla: 3.869 ± 0.897
0.27ThrCys: 0.27 ± 0.142
3.059ThrAsp: 3.059 ± 0.613
2.969ThrGlu: 2.969 ± 0.438
2.519ThrPhe: 2.519 ± 0.415
4.049ThrGly: 4.049 ± 0.566
0.36ThrHis: 0.36 ± 0.211
4.318ThrIle: 4.318 ± 0.692
5.128ThrLys: 5.128 ± 0.653
6.298ThrLeu: 6.298 ± 0.844
0.9ThrMet: 0.9 ± 0.348
3.149ThrAsn: 3.149 ± 0.558
2.789ThrPro: 2.789 ± 0.526
1.889ThrGln: 1.889 ± 0.353
2.069ThrArg: 2.069 ± 0.427
3.599ThrSer: 3.599 ± 0.938
5.038ThrThr: 5.038 ± 0.715
2.429ThrVal: 2.429 ± 0.399
0.45ThrTrp: 0.45 ± 0.264
3.329ThrTyr: 3.329 ± 0.502
0.0ThrXaa: 0.0 ± 0.0
Val
4.408ValAla: 4.408 ± 0.736
0.36ValCys: 0.36 ± 0.173
4.139ValAsp: 4.139 ± 0.462
4.588ValGlu: 4.588 ± 0.685
2.429ValPhe: 2.429 ± 0.359
4.588ValGly: 4.588 ± 0.7
1.26ValHis: 1.26 ± 0.319
3.959ValIle: 3.959 ± 0.617
5.938ValLys: 5.938 ± 0.738
4.948ValLeu: 4.948 ± 0.745
1.26ValMet: 1.26 ± 0.329
2.339ValAsn: 2.339 ± 0.344
1.799ValPro: 1.799 ± 0.372
2.429ValGln: 2.429 ± 0.529
1.979ValArg: 1.979 ± 0.363
3.329ValSer: 3.329 ± 0.453
3.419ValThr: 3.419 ± 0.729
4.049ValVal: 4.049 ± 0.574
0.45ValTrp: 0.45 ± 0.236
1.889ValTyr: 1.889 ± 0.515
0.0ValXaa: 0.0 ± 0.0
Trp
1.26TrpAla: 1.26 ± 0.32
0.09TrpCys: 0.09 ± 0.095
0.54TrpAsp: 0.54 ± 0.193
0.9TrpGlu: 0.9 ± 0.256
0.54TrpPhe: 0.54 ± 0.217
0.45TrpGly: 0.45 ± 0.179
0.09TrpHis: 0.09 ± 0.079
1.35TrpIle: 1.35 ± 0.358
1.35TrpLys: 1.35 ± 0.377
1.08TrpLeu: 1.08 ± 0.251
0.36TrpMet: 0.36 ± 0.166
0.45TrpAsn: 0.45 ± 0.212
0.18TrpPro: 0.18 ± 0.119
0.45TrpGln: 0.45 ± 0.205
0.45TrpArg: 0.45 ± 0.262
0.81TrpSer: 0.81 ± 0.262
1.17TrpThr: 1.17 ± 0.34
1.26TrpVal: 1.26 ± 0.4
0.09TrpTrp: 0.09 ± 0.095
0.27TrpTyr: 0.27 ± 0.152
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.329TyrAla: 3.329 ± 0.725
0.09TyrCys: 0.09 ± 0.1
3.329TyrAsp: 3.329 ± 0.687
3.239TyrGlu: 3.239 ± 0.462
1.26TyrPhe: 1.26 ± 0.334
2.609TyrGly: 2.609 ± 0.556
0.54TyrHis: 0.54 ± 0.212
3.689TyrIle: 3.689 ± 0.566
4.318TyrLys: 4.318 ± 0.52
4.229TyrLeu: 4.229 ± 0.581
0.63TyrMet: 0.63 ± 0.291
2.609TyrAsn: 2.609 ± 0.437
0.63TyrPro: 0.63 ± 0.209
2.609TyrGln: 2.609 ± 0.569
1.17TyrArg: 1.17 ± 0.328
2.609TyrSer: 2.609 ± 0.636
2.429TyrThr: 2.429 ± 0.532
2.249TyrVal: 2.249 ± 0.446
0.36TyrTrp: 0.36 ± 0.201
1.439TyrTyr: 1.439 ± 0.383
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (11116 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski