Fusarium poae mitovirus 4
Average proteome isoelectric point is 8.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4ZA33|A0A1B4ZA33_9VIRU RNA-dependent RNA polymerase OS=Fusarium poae mitovirus 4 OX=1848153 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.79 NHH4 pKa = 6.02 MKK6 pKa = 10.28 LVKK9 pKa = 10.19 NLGFKK14 pKa = 10.26 RR15 pKa = 11.84 VEE17 pKa = 4.29 GGTKK21 pKa = 10.06 FLNIKK26 pKa = 8.54 EE27 pKa = 4.3 LPRR30 pKa = 11.84 FTRR33 pKa = 11.84 LLIWLLASNDD43 pKa = 3.65 EE44 pKa = 4.15 KK45 pKa = 11.57 SSLFEE50 pKa = 3.46 LALRR54 pKa = 11.84 IKK56 pKa = 10.57 RR57 pKa = 11.84 MHH59 pKa = 6.67 NSNGPLFTVMYY70 pKa = 9.53 LKK72 pKa = 10.45 EE73 pKa = 4.16 SHH75 pKa = 6.92 RR76 pKa = 11.84 LTMKK80 pKa = 10.87 AIGGQQEE87 pKa = 4.39 TCTTFPRR94 pKa = 11.84 VATRR98 pKa = 11.84 RR99 pKa = 11.84 GLPLIVPGDD108 pKa = 3.42 LRR110 pKa = 11.84 VRR112 pKa = 11.84 MEE114 pKa = 4.14 KK115 pKa = 10.9 SNFDD119 pKa = 3.71 TIRR122 pKa = 11.84 AVLSLLTVYY131 pKa = 10.39 RR132 pKa = 11.84 VINCASTLKK141 pKa = 10.54 LQTITDD147 pKa = 4.09 PFKK150 pKa = 10.27 GTVDD154 pKa = 3.14 RR155 pKa = 11.84 FTSLEE160 pKa = 4.05 LEE162 pKa = 4.57 KK163 pKa = 10.69 GFKK166 pKa = 8.9 MLRR169 pKa = 11.84 LKK171 pKa = 11.08 GSMVLEE177 pKa = 4.13 EE178 pKa = 4.57 NKK180 pKa = 10.35 FLIPSVKK187 pKa = 10.28 SGPNYY192 pKa = 10.16 KK193 pKa = 9.61 IAALGATLDD202 pKa = 3.56 AKK204 pKa = 10.94 AFSEE208 pKa = 4.15 DD209 pKa = 3.69 SRR211 pKa = 11.84 LLSYY215 pKa = 11.39 AEE217 pKa = 4.06 TVSAVTAPSLFSLLKK232 pKa = 10.83 EE233 pKa = 4.38 EE234 pKa = 4.38 INNLGSWSSTMKK246 pKa = 10.81 EE247 pKa = 4.03 EE248 pKa = 4.65 LLSKK252 pKa = 8.15 YY253 pKa = 9.06 TRR255 pKa = 11.84 DD256 pKa = 3.42 LKK258 pKa = 10.96 LGKK261 pKa = 10.31 LSEE264 pKa = 4.3 KK265 pKa = 10.58 KK266 pKa = 9.5 EE267 pKa = 3.89 AAGKK271 pKa = 10.05 VRR273 pKa = 11.84 VFAITDD279 pKa = 2.91 VWTQSFLSPLHH290 pKa = 5.8 HH291 pKa = 7.37 AIFGFLKK298 pKa = 10.16 RR299 pKa = 11.84 IPMDD303 pKa = 3.3 GTFDD307 pKa = 3.63 QLKK310 pKa = 9.5 PLNALLSRR318 pKa = 11.84 GLKK321 pKa = 10.14 NFYY324 pKa = 10.73 SYY326 pKa = 11.4 DD327 pKa = 3.28 LSAATDD333 pKa = 3.71 RR334 pKa = 11.84 LPITLQEE341 pKa = 4.26 QILSRR346 pKa = 11.84 LFGEE350 pKa = 4.85 SFAKK354 pKa = 9.68 AWKK357 pKa = 9.26 GLLVEE362 pKa = 4.67 RR363 pKa = 11.84 PWYY366 pKa = 10.25 HH367 pKa = 6.7 KK368 pKa = 10.26 GIPYY372 pKa = 9.9 LYY374 pKa = 10.64 SVGQPMGALSSWGMLALTHH393 pKa = 6.49 HH394 pKa = 7.01 MIVQVAASRR403 pKa = 11.84 VGHH406 pKa = 5.19 RR407 pKa = 11.84 TMFRR411 pKa = 11.84 DD412 pKa = 3.61 YY413 pKa = 11.56 ALLGDD418 pKa = 5.54 DD419 pKa = 4.01 ICIADD424 pKa = 3.72 SAVAKK429 pKa = 10.43 SYY431 pKa = 11.13 LSLMTDD437 pKa = 3.16 YY438 pKa = 11.28 GVDD441 pKa = 3.28 INLSKK446 pKa = 10.95 SLEE449 pKa = 4.04 SDD451 pKa = 2.84 IGVAEE456 pKa = 4.15 FAKK459 pKa = 10.61 RR460 pKa = 11.84 LIKK463 pKa = 10.8 DD464 pKa = 3.55 EE465 pKa = 4.52 TDD467 pKa = 3.89 LSPLPPKK474 pKa = 10.72 LITLLMSQFKK484 pKa = 10.5 ALPTLVRR491 pKa = 11.84 DD492 pKa = 3.86 MIGRR496 pKa = 11.84 GLSVEE501 pKa = 4.39 SLLKK505 pKa = 11.0 DD506 pKa = 3.24 EE507 pKa = 4.88 TRR509 pKa = 11.84 VSRR512 pKa = 11.84 PILWEE517 pKa = 4.31 LIGPLGLLPSAGLSPFLGDD536 pKa = 3.78 RR537 pKa = 11.84 SLTKK541 pKa = 10.71 DD542 pKa = 3.42 EE543 pKa = 4.58 LRR545 pKa = 11.84 VVADD549 pKa = 3.54 CVSRR553 pKa = 11.84 VINRR557 pKa = 11.84 WIIRR561 pKa = 11.84 YY562 pKa = 8.43 FYY564 pKa = 10.8 QNQQTSQEE572 pKa = 4.34 LIEE575 pKa = 5.01 KK576 pKa = 9.95 IGSLVWDD583 pKa = 4.44 PSNGINRR590 pKa = 11.84 DD591 pKa = 3.16 TPAFHH596 pKa = 7.46 HH597 pKa = 6.14 YY598 pKa = 8.74 MNSFIEE604 pKa = 3.99 ITIEE608 pKa = 3.79 EE609 pKa = 4.22 NTAQPEE615 pKa = 4.47 LVNFPPDD622 pKa = 3.26 RR623 pKa = 11.84 EE624 pKa = 4.0 ASFEE628 pKa = 3.98 NVYY631 pKa = 9.21 TFIKK635 pKa = 10.29 EE636 pKa = 4.18 AMDD639 pKa = 3.98 HH640 pKa = 6.71 FDD642 pKa = 3.55 GLAPAVPDD650 pKa = 3.28 ISEE653 pKa = 4.2 KK654 pKa = 10.42 PRR656 pKa = 11.84 VRR658 pKa = 11.84 PISASSKK665 pKa = 8.02 MKK667 pKa = 10.44 FYY669 pKa = 11.2 QEE671 pKa = 4.09 LNRR674 pKa = 11.84 ALQDD678 pKa = 3.05 TGINFEE684 pKa = 4.38 YY685 pKa = 9.76 LTNNN689 pKa = 3.36
Molecular weight: 77.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.649
IPC2_protein 8.551
IPC_protein 8.434
Toseland 9.224
ProMoST 8.99
Dawson 9.502
Bjellqvist 9.224
Wikipedia 9.663
Rodwell 9.78
Grimsley 9.575
Solomon 9.531
Lehninger 9.502
Nozaki 9.326
DTASelect 9.18
Thurlkill 9.355
EMBOSS 9.663
Sillero 9.458
Patrickios 5.016
IPC_peptide 9.531
IPC2_peptide 7.717
IPC2.peptide.svr19 7.699
Protein with the highest isoelectric point:
>tr|A0A1B4ZA33|A0A1B4ZA33_9VIRU RNA-dependent RNA polymerase OS=Fusarium poae mitovirus 4 OX=1848153 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.79 NHH4 pKa = 6.02 MKK6 pKa = 10.28 LVKK9 pKa = 10.19 NLGFKK14 pKa = 10.26 RR15 pKa = 11.84 VEE17 pKa = 4.29 GGTKK21 pKa = 10.06 FLNIKK26 pKa = 8.54 EE27 pKa = 4.3 LPRR30 pKa = 11.84 FTRR33 pKa = 11.84 LLIWLLASNDD43 pKa = 3.65 EE44 pKa = 4.15 KK45 pKa = 11.57 SSLFEE50 pKa = 3.46 LALRR54 pKa = 11.84 IKK56 pKa = 10.57 RR57 pKa = 11.84 MHH59 pKa = 6.67 NSNGPLFTVMYY70 pKa = 9.53 LKK72 pKa = 10.45 EE73 pKa = 4.16 SHH75 pKa = 6.92 RR76 pKa = 11.84 LTMKK80 pKa = 10.87 AIGGQQEE87 pKa = 4.39 TCTTFPRR94 pKa = 11.84 VATRR98 pKa = 11.84 RR99 pKa = 11.84 GLPLIVPGDD108 pKa = 3.42 LRR110 pKa = 11.84 VRR112 pKa = 11.84 MEE114 pKa = 4.14 KK115 pKa = 10.9 SNFDD119 pKa = 3.71 TIRR122 pKa = 11.84 AVLSLLTVYY131 pKa = 10.39 RR132 pKa = 11.84 VINCASTLKK141 pKa = 10.54 LQTITDD147 pKa = 4.09 PFKK150 pKa = 10.27 GTVDD154 pKa = 3.14 RR155 pKa = 11.84 FTSLEE160 pKa = 4.05 LEE162 pKa = 4.57 KK163 pKa = 10.69 GFKK166 pKa = 8.9 MLRR169 pKa = 11.84 LKK171 pKa = 11.08 GSMVLEE177 pKa = 4.13 EE178 pKa = 4.57 NKK180 pKa = 10.35 FLIPSVKK187 pKa = 10.28 SGPNYY192 pKa = 10.16 KK193 pKa = 9.61 IAALGATLDD202 pKa = 3.56 AKK204 pKa = 10.94 AFSEE208 pKa = 4.15 DD209 pKa = 3.69 SRR211 pKa = 11.84 LLSYY215 pKa = 11.39 AEE217 pKa = 4.06 TVSAVTAPSLFSLLKK232 pKa = 10.83 EE233 pKa = 4.38 EE234 pKa = 4.38 INNLGSWSSTMKK246 pKa = 10.81 EE247 pKa = 4.03 EE248 pKa = 4.65 LLSKK252 pKa = 8.15 YY253 pKa = 9.06 TRR255 pKa = 11.84 DD256 pKa = 3.42 LKK258 pKa = 10.96 LGKK261 pKa = 10.31 LSEE264 pKa = 4.3 KK265 pKa = 10.58 KK266 pKa = 9.5 EE267 pKa = 3.89 AAGKK271 pKa = 10.05 VRR273 pKa = 11.84 VFAITDD279 pKa = 2.91 VWTQSFLSPLHH290 pKa = 5.8 HH291 pKa = 7.37 AIFGFLKK298 pKa = 10.16 RR299 pKa = 11.84 IPMDD303 pKa = 3.3 GTFDD307 pKa = 3.63 QLKK310 pKa = 9.5 PLNALLSRR318 pKa = 11.84 GLKK321 pKa = 10.14 NFYY324 pKa = 10.73 SYY326 pKa = 11.4 DD327 pKa = 3.28 LSAATDD333 pKa = 3.71 RR334 pKa = 11.84 LPITLQEE341 pKa = 4.26 QILSRR346 pKa = 11.84 LFGEE350 pKa = 4.85 SFAKK354 pKa = 9.68 AWKK357 pKa = 9.26 GLLVEE362 pKa = 4.67 RR363 pKa = 11.84 PWYY366 pKa = 10.25 HH367 pKa = 6.7 KK368 pKa = 10.26 GIPYY372 pKa = 9.9 LYY374 pKa = 10.64 SVGQPMGALSSWGMLALTHH393 pKa = 6.49 HH394 pKa = 7.01 MIVQVAASRR403 pKa = 11.84 VGHH406 pKa = 5.19 RR407 pKa = 11.84 TMFRR411 pKa = 11.84 DD412 pKa = 3.61 YY413 pKa = 11.56 ALLGDD418 pKa = 5.54 DD419 pKa = 4.01 ICIADD424 pKa = 3.72 SAVAKK429 pKa = 10.43 SYY431 pKa = 11.13 LSLMTDD437 pKa = 3.16 YY438 pKa = 11.28 GVDD441 pKa = 3.28 INLSKK446 pKa = 10.95 SLEE449 pKa = 4.04 SDD451 pKa = 2.84 IGVAEE456 pKa = 4.15 FAKK459 pKa = 10.61 RR460 pKa = 11.84 LIKK463 pKa = 10.8 DD464 pKa = 3.55 EE465 pKa = 4.52 TDD467 pKa = 3.89 LSPLPPKK474 pKa = 10.72 LITLLMSQFKK484 pKa = 10.5 ALPTLVRR491 pKa = 11.84 DD492 pKa = 3.86 MIGRR496 pKa = 11.84 GLSVEE501 pKa = 4.39 SLLKK505 pKa = 11.0 DD506 pKa = 3.24 EE507 pKa = 4.88 TRR509 pKa = 11.84 VSRR512 pKa = 11.84 PILWEE517 pKa = 4.31 LIGPLGLLPSAGLSPFLGDD536 pKa = 3.78 RR537 pKa = 11.84 SLTKK541 pKa = 10.71 DD542 pKa = 3.42 EE543 pKa = 4.58 LRR545 pKa = 11.84 VVADD549 pKa = 3.54 CVSRR553 pKa = 11.84 VINRR557 pKa = 11.84 WIIRR561 pKa = 11.84 YY562 pKa = 8.43 FYY564 pKa = 10.8 QNQQTSQEE572 pKa = 4.34 LIEE575 pKa = 5.01 KK576 pKa = 9.95 IGSLVWDD583 pKa = 4.44 PSNGINRR590 pKa = 11.84 DD591 pKa = 3.16 TPAFHH596 pKa = 7.46 HH597 pKa = 6.14 YY598 pKa = 8.74 MNSFIEE604 pKa = 3.99 ITIEE608 pKa = 3.79 EE609 pKa = 4.22 NTAQPEE615 pKa = 4.47 LVNFPPDD622 pKa = 3.26 RR623 pKa = 11.84 EE624 pKa = 4.0 ASFEE628 pKa = 3.98 NVYY631 pKa = 9.21 TFIKK635 pKa = 10.29 EE636 pKa = 4.18 AMDD639 pKa = 3.98 HH640 pKa = 6.71 FDD642 pKa = 3.55 GLAPAVPDD650 pKa = 3.28 ISEE653 pKa = 4.2 KK654 pKa = 10.42 PRR656 pKa = 11.84 VRR658 pKa = 11.84 PISASSKK665 pKa = 8.02 MKK667 pKa = 10.44 FYY669 pKa = 11.2 QEE671 pKa = 4.09 LNRR674 pKa = 11.84 ALQDD678 pKa = 3.05 TGINFEE684 pKa = 4.38 YY685 pKa = 9.76 LTNNN689 pKa = 3.36
Molecular weight: 77.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.649
IPC2_protein 8.551
IPC_protein 8.434
Toseland 9.224
ProMoST 8.99
Dawson 9.502
Bjellqvist 9.224
Wikipedia 9.663
Rodwell 9.78
Grimsley 9.575
Solomon 9.531
Lehninger 9.502
Nozaki 9.326
DTASelect 9.18
Thurlkill 9.355
EMBOSS 9.663
Sillero 9.458
Patrickios 5.016
IPC_peptide 9.531
IPC2_peptide 7.717
IPC2.peptide.svr19 7.699
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
689
689
689
689.0
77.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.386 ± 0.0
0.581 ± 0.0
4.935 ± 0.0
5.951 ± 0.0
4.935 ± 0.0
5.66 ± 0.0
1.742 ± 0.0
5.806 ± 0.0
6.821 ± 0.0
13.498 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.903 ± 0.0
3.919 ± 0.0
4.79 ± 0.0
2.467 ± 0.0
5.951 ± 0.0
8.418 ± 0.0
5.806 ± 0.0
5.37 ± 0.0
1.306 ± 0.0
2.758 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here