Lake Sarah-associated circular virus-26

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQM6|A0A140AQM6_9VIRU Putative capsid protein OS=Lake Sarah-associated circular virus-26 OX=1685753 PE=4 SV=1
MM1 pKa = 7.29VFNFNAKK8 pKa = 9.62HH9 pKa = 5.64VFLTYY14 pKa = 10.18PQTTLTKK21 pKa = 9.01EE22 pKa = 4.01ALYY25 pKa = 9.95EE26 pKa = 3.86ASIRR30 pKa = 11.84WGCVSGTIGQEE41 pKa = 3.54KK42 pKa = 9.11HH43 pKa = 5.53QDD45 pKa = 3.48GGLHH49 pKa = 5.76LHH51 pKa = 6.64AVFAFDD57 pKa = 3.6RR58 pKa = 11.84KK59 pKa = 9.47RR60 pKa = 11.84HH61 pKa = 5.26IRR63 pKa = 11.84DD64 pKa = 2.93EE65 pKa = 4.32RR66 pKa = 11.84FFDD69 pKa = 4.49LEE71 pKa = 4.61GYY73 pKa = 9.35HH74 pKa = 6.99PNVQSARR81 pKa = 11.84NVKK84 pKa = 9.82AVRR87 pKa = 11.84EE88 pKa = 4.14YY89 pKa = 11.14CEE91 pKa = 4.99KK92 pKa = 10.54EE93 pKa = 3.88DD94 pKa = 4.8PEE96 pKa = 4.12ALRR99 pKa = 11.84YY100 pKa = 9.26GAFEE104 pKa = 4.29GCEE107 pKa = 3.96KK108 pKa = 10.33IDD110 pKa = 3.47WKK112 pKa = 11.21DD113 pKa = 3.66LLGLATDD120 pKa = 3.65SAHH123 pKa = 7.15FIKK126 pKa = 10.7LVTDD130 pKa = 4.05RR131 pKa = 11.84DD132 pKa = 3.53VSFCVKK138 pKa = 10.13HH139 pKa = 5.77FSNVRR144 pKa = 11.84AFAEE148 pKa = 4.15YY149 pKa = 9.48TFRR152 pKa = 11.84SRR154 pKa = 11.84KK155 pKa = 9.28EE156 pKa = 3.75DD157 pKa = 3.43FTPQFTDD164 pKa = 3.43FRR166 pKa = 11.84NQLRR170 pKa = 11.84PMQDD174 pKa = 2.62FHH176 pKa = 9.58AEE178 pKa = 3.98LLRR181 pKa = 11.84PSRR184 pKa = 11.84PRR186 pKa = 11.84EE187 pKa = 3.53RR188 pKa = 11.84HH189 pKa = 3.95TSIVIVGATRR199 pKa = 11.84LGKK202 pKa = 8.09TEE204 pKa = 3.84WARR207 pKa = 11.84SLGNHH212 pKa = 6.34TYY214 pKa = 10.19WSGAVDD220 pKa = 3.41WTRR223 pKa = 11.84HH224 pKa = 5.6DD225 pKa = 3.29NHH227 pKa = 6.5SRR229 pKa = 11.84YY230 pKa = 10.08AVIDD234 pKa = 4.21DD235 pKa = 4.97LSLEE239 pKa = 4.3YY240 pKa = 10.01IPRR243 pKa = 11.84LQQIFGCQHH252 pKa = 6.6NISVSQKK259 pKa = 8.93YY260 pKa = 9.28KK261 pKa = 10.71PIMTFDD267 pKa = 2.79WGIPVIYY274 pKa = 8.99LTNDD278 pKa = 2.72EE279 pKa = 5.12NYY281 pKa = 10.67LEE283 pKa = 4.45KK284 pKa = 10.85QPDD287 pKa = 4.47FIKK290 pKa = 10.51SWWQNNVVTCHH301 pKa = 5.17VRR303 pKa = 11.84NKK305 pKa = 10.76LFVV308 pKa = 3.33

Molecular weight: 36.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQM6|A0A140AQM6_9VIRU Putative capsid protein OS=Lake Sarah-associated circular virus-26 OX=1685753 PE=4 SV=1
MM1 pKa = 7.46VKK3 pKa = 10.05RR4 pKa = 11.84KK5 pKa = 10.05YY6 pKa = 10.56SATGYY11 pKa = 9.82ARR13 pKa = 11.84NYY15 pKa = 8.92GKK17 pKa = 10.0NVLTAARR24 pKa = 11.84TLMSLRR30 pKa = 11.84NGFKK34 pKa = 10.42SGSRR38 pKa = 11.84GPNSTNNRR46 pKa = 11.84RR47 pKa = 11.84SFNSTGITTDD57 pKa = 3.35HH58 pKa = 6.73YY59 pKa = 11.61DD60 pKa = 2.94KK61 pKa = 10.16KK62 pKa = 9.42TKK64 pKa = 10.19YY65 pKa = 9.35RR66 pKa = 11.84YY67 pKa = 10.14KK68 pKa = 10.5RR69 pKa = 11.84MPYY72 pKa = 9.65RR73 pKa = 11.84KK74 pKa = 8.23RR75 pKa = 11.84KK76 pKa = 9.33RR77 pKa = 11.84YY78 pKa = 8.42VRR80 pKa = 11.84RR81 pKa = 11.84LKK83 pKa = 10.31FVKK86 pKa = 10.16HH87 pKa = 5.99ALSKK91 pKa = 11.02DD92 pKa = 2.97LATFKK97 pKa = 8.53TTYY100 pKa = 8.97ATSTAVTALAGAQNVFATFSVMGVPVTGANLQTAGNFNQTINAWQNMSGTIDD152 pKa = 3.28ASTAGDD158 pKa = 3.52HH159 pKa = 6.87YY160 pKa = 11.19GEE162 pKa = 4.25KK163 pKa = 9.51WKK165 pKa = 10.54QLSSVVNVSITNYY178 pKa = 8.49GTTTVIMDD186 pKa = 3.75VYY188 pKa = 10.72RR189 pKa = 11.84VVCRR193 pKa = 11.84KK194 pKa = 7.77TFSNSDD200 pKa = 3.34FSSPEE205 pKa = 3.85TTNGSSEE212 pKa = 4.24TVLMKK217 pKa = 10.81KK218 pKa = 9.91MSDD221 pKa = 3.44NLSGTVSNDD230 pKa = 2.59WEE232 pKa = 4.82TVGITPFAINAFTKK246 pKa = 10.1HH247 pKa = 5.92FKK249 pKa = 10.06ILQVKK254 pKa = 8.38EE255 pKa = 3.96VQIPAGNTTTLSFRR269 pKa = 11.84DD270 pKa = 3.53PKK272 pKa = 10.93NYY274 pKa = 9.85SFNMSQFLGKK284 pKa = 10.5VFMKK288 pKa = 10.55GLTKK292 pKa = 10.49GYY294 pKa = 9.65VYY296 pKa = 10.62RR297 pKa = 11.84FRR299 pKa = 11.84AIAATAGGEE308 pKa = 4.13SAAISMGVHH317 pKa = 5.87EE318 pKa = 5.41EE319 pKa = 3.96YY320 pKa = 11.38SNIVQEE326 pKa = 4.4LEE328 pKa = 3.96PTEE331 pKa = 4.12SLNYY335 pKa = 9.51HH336 pKa = 6.11ANKK339 pKa = 8.74TT340 pKa = 3.73

Molecular weight: 37.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

648

308

340

324.0

37.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.944 ± 0.75

1.08 ± 0.592

4.63 ± 1.271

4.63 ± 1.049

5.864 ± 0.65

6.019 ± 0.783

3.241 ± 1.111

4.167 ± 0.48

6.79 ± 0.866

6.019 ± 0.767

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.16 ± 0.809

6.173 ± 1.11

2.932 ± 0.436

3.395 ± 0.563

6.636 ± 0.788

7.253 ± 1.403

8.796 ± 2.013

7.099 ± 0.191

1.698 ± 0.614

4.475 ± 0.395
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski