Stx2-converting phage 1717

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pankowvirus; Escherichia virus 1717

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6DZT7|B6DZT7_9CAUD N protein OS=Stx2-converting phage 1717 OX=563769 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 4.36FHH4 pKa = 7.21EE5 pKa = 5.34SAICDD10 pKa = 3.52FRR12 pKa = 11.84ANANSVKK19 pKa = 9.25PQPIAVLFKK28 pKa = 10.94TMGAWAVLCFAADD41 pKa = 3.76DD42 pKa = 4.28TDD44 pKa = 3.9DD45 pKa = 3.38TDD47 pKa = 3.53ARR49 pKa = 11.84MAIGQEE55 pKa = 4.02MEE57 pKa = 3.99MDD59 pKa = 3.55PTNDD63 pKa = 2.86EE64 pKa = 4.58FIIYY68 pKa = 7.71GAPSNYY74 pKa = 10.43LLDD77 pKa = 3.47TCNIYY82 pKa = 10.99NKK84 pKa = 10.37AAA86 pKa = 3.64

Molecular weight: 9.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6DZX1|B6DZX1_9CAUD Restriction endonuclease OS=Stx2-converting phage 1717 OX=563769 PE=4 SV=1
MM1 pKa = 7.33SSKK4 pKa = 10.49NRR6 pKa = 11.84TRR8 pKa = 11.84RR9 pKa = 11.84TTTRR13 pKa = 11.84NIRR16 pKa = 11.84FRR18 pKa = 11.84NQMIEE23 pKa = 4.08QINIALTRR31 pKa = 11.84KK32 pKa = 6.96VQKK35 pKa = 10.34IFLRR39 pKa = 11.84GSLNLAAGSWQQTT52 pKa = 3.39

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

16574

44

1159

215.2

23.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.382 ± 0.55

1.448 ± 0.164

5.484 ± 0.22

6.492 ± 0.275

3.228 ± 0.159

7.204 ± 0.35

2.027 ± 0.161

5.316 ± 0.269

5.43 ± 0.287

8.218 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.709 ± 0.188

4.32 ± 0.213

4.133 ± 0.283

4.32 ± 0.26

6.908 ± 0.319

6.836 ± 0.212

5.708 ± 0.349

6.42 ± 0.26

1.695 ± 0.121

2.721 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski