Veillonella sp. AS16
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1736 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W3Y561|W3Y561_9FIRM 50S ribosomal protein L21 OS=Veillonella sp. AS16 OX=936589 GN=rplU PE=3 SV=1
MM1 pKa = 7.52 TEE3 pKa = 3.6 IQALLLLKK11 pKa = 10.73 ASLEE15 pKa = 4.24 SADD18 pKa = 3.25 IHH20 pKa = 5.8 GVEE23 pKa = 5.41 SILSDD28 pKa = 3.35 DD29 pKa = 4.49 CEE31 pKa = 4.38 YY32 pKa = 10.69 MSTGRR37 pKa = 11.84 GIIGRR42 pKa = 11.84 NRR44 pKa = 11.84 SEE46 pKa = 4.24 TIDD49 pKa = 3.76 FLSSMMEE56 pKa = 4.09 SIKK59 pKa = 10.7 ADD61 pKa = 3.37 RR62 pKa = 11.84 VPVNCNVMHH71 pKa = 6.23 ITDD74 pKa = 3.77 VYY76 pKa = 11.29 EE77 pKa = 4.36 EE78 pKa = 4.52 GALFATGRR86 pKa = 11.84 HH87 pKa = 5.28 GLSVSYY93 pKa = 10.5 EE94 pKa = 4.03 SGDD97 pKa = 3.5 TYY99 pKa = 11.61 VYY101 pKa = 10.58 MLFIDD106 pKa = 4.09 VNDD109 pKa = 3.73 EE110 pKa = 4.07 GLVDD114 pKa = 5.33 RR115 pKa = 11.84 IVSSQEE121 pKa = 3.84 NYY123 pKa = 10.59 SIAYY127 pKa = 7.48 DD128 pKa = 3.75 TLRR131 pKa = 11.84 LNDD134 pKa = 4.96 DD135 pKa = 3.51 EE136 pKa = 5.07 PEE138 pKa = 4.31 YY139 pKa = 11.37 AFTTAPTTVKK149 pKa = 10.7 DD150 pKa = 3.72 WITALSIWLEE160 pKa = 4.04 TDD162 pKa = 3.02 NVDD165 pKa = 3.11 IDD167 pKa = 3.75 DD168 pKa = 4.73 FYY170 pKa = 11.67 RR171 pKa = 11.84 YY172 pKa = 8.99 IDD174 pKa = 3.51 EE175 pKa = 4.23 DD176 pKa = 3.93 TRR178 pKa = 11.84 VVFQKK183 pKa = 11.05 GDD185 pKa = 3.45 AEE187 pKa = 5.22 SITLEE192 pKa = 4.12 NGLDD196 pKa = 3.43 VEE198 pKa = 5.73 DD199 pKa = 5.74 YY200 pKa = 10.97 FDD202 pKa = 4.19 QLMNQHH208 pKa = 6.37 FDD210 pKa = 3.52 AVPHH214 pKa = 7.17 IIRR217 pKa = 11.84 DD218 pKa = 3.73 DD219 pKa = 4.22 DD220 pKa = 4.8 DD221 pKa = 5.26 NLILTYY227 pKa = 11.14 GPMSLIATLNEE238 pKa = 4.35 DD239 pKa = 3.35 GALALISIYY248 pKa = 10.26 IDD250 pKa = 3.02 IRR252 pKa = 11.84 DD253 pKa = 3.77 EE254 pKa = 4.24 DD255 pKa = 3.95 VSDD258 pKa = 3.36 EE259 pKa = 4.1 VGYY262 pKa = 10.4 IEE264 pKa = 5.47 EE265 pKa = 5.98 DD266 pKa = 3.01 ITEE269 pKa = 4.66 IIYY272 pKa = 10.73 DD273 pKa = 4.15 DD274 pKa = 3.87 EE275 pKa = 7.39 DD276 pKa = 3.65 ITEE279 pKa = 4.13 VAHH282 pKa = 7.89 DD283 pKa = 4.3 DD284 pKa = 4.11 FEE286 pKa = 4.57
Molecular weight: 32.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.49
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.884
Wikipedia 3.643
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.656
Nozaki 3.821
DTASelect 4.062
Thurlkill 3.541
EMBOSS 3.656
Sillero 3.834
Patrickios 1.303
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|W3Y1K6|W3Y1K6_9FIRM BMC domain protein OS=Veillonella sp. AS16 OX=936589 GN=HMPREF1521_0856 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPNTLWRR12 pKa = 11.84 KK13 pKa = 6.72 RR14 pKa = 11.84 THH16 pKa = 6.25 GFRR19 pKa = 11.84 EE20 pKa = 3.78 RR21 pKa = 11.84 MKK23 pKa = 10.34 TIGGRR28 pKa = 11.84 LVLKK32 pKa = 10.36 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.84 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.334
IPC2_protein 10.862
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.164
Grimsley 12.486
Solomon 12.925
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.901
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.055
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1736
0
1736
572448
34
2862
329.8
36.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.23 ± 0.066
1.035 ± 0.022
5.876 ± 0.047
6.316 ± 0.068
3.779 ± 0.045
7.589 ± 0.069
2.235 ± 0.032
7.486 ± 0.063
5.851 ± 0.056
9.002 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.008 ± 0.031
4.293 ± 0.063
3.699 ± 0.036
3.353 ± 0.037
4.526 ± 0.05
5.683 ± 0.043
5.798 ± 0.061
7.747 ± 0.05
0.9 ± 0.022
3.594 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here