Amino acid dipepetide frequency for Nitrospira japonica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.107AlaAla: 11.107 ± 0.127	1.098AlaCys: 1.098 ± 0.029	5.112AlaAsp: 5.112 ± 0.07	5.747AlaGlu: 5.747 ± 0.065	3.365AlaPhe: 3.365 ± 0.056	8.459AlaGly: 8.459 ± 0.117	2.0AlaHis: 2.0 ± 0.041	4.849AlaIle: 4.849 ± 0.069	4.07AlaLys: 4.07 ± 0.079	10.407AlaLeu: 10.407 ± 0.122	2.622AlaMet: 2.622 ± 0.049	2.412AlaAsn: 2.412 ± 0.083	4.282AlaPro: 4.282 ± 0.074	3.653AlaGln: 3.653 ± 0.062	6.393AlaArg: 6.393 ± 0.072	5.799AlaSer: 5.799 ± 0.078	4.984AlaThr: 4.984 ± 0.083	8.111AlaVal: 8.111 ± 0.084	1.361AlaTrp: 1.361 ± 0.037	2.424AlaTyr: 2.424 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.936CysAla: 0.936 ± 0.03	0.151CysCys: 0.151 ± 0.012	0.462CysAsp: 0.462 ± 0.019	0.466CysGlu: 0.466 ± 0.021	0.396CysPhe: 0.396 ± 0.018	0.984CysGly: 0.984 ± 0.029	0.354CysHis: 0.354 ± 0.022	0.372CysIle: 0.372 ± 0.018	0.301CysLys: 0.301 ± 0.015	1.146CysLeu: 1.146 ± 0.035	0.204CysMet: 0.204 ± 0.012	0.232CysAsn: 0.232 ± 0.012	0.615CysPro: 0.615 ± 0.027	0.342CysGln: 0.342 ± 0.017	0.897CysArg: 0.897 ± 0.027	0.654CysSer: 0.654 ± 0.023	0.484CysThr: 0.484 ± 0.023	0.732CysVal: 0.732 ± 0.026	0.145CysTrp: 0.145 ± 0.011	0.261CysTyr: 0.261 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	4.98AspAla: 4.98 ± 0.068	0.524AspCys: 0.524 ± 0.021	2.555AspAsp: 2.555 ± 0.052	3.236AspGlu: 3.236 ± 0.056	1.821AspPhe: 1.821 ± 0.037	4.432AspGly: 4.432 ± 0.078	1.39AspHis: 1.39 ± 0.037	2.592AspIle: 2.592 ± 0.053	1.695AspLys: 1.695 ± 0.04	5.787AspLeu: 5.787 ± 0.077	1.094AspMet: 1.094 ± 0.029	1.144AspAsn: 1.144 ± 0.033	3.352AspPro: 3.352 ± 0.059	2.198AspGln: 2.198 ± 0.041	4.618AspArg: 4.618 ± 0.069	2.672AspSer: 2.672 ± 0.048	2.342AspThr: 2.342 ± 0.05	4.207AspVal: 4.207 ± 0.061	0.784AspTrp: 0.784 ± 0.028	1.474AspTyr: 1.474 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	6.28GluAla: 6.28 ± 0.072	0.47GluCys: 0.47 ± 0.023	2.724GluAsp: 2.724 ± 0.047	3.767GluGlu: 3.767 ± 0.071	2.08GluPhe: 2.08 ± 0.038	4.065GluGly: 4.065 ± 0.063	1.416GluHis: 1.416 ± 0.037	3.14GluIle: 3.14 ± 0.054	2.41GluLys: 2.41 ± 0.058	5.897GluLeu: 5.897 ± 0.088	1.376GluMet: 1.376 ± 0.033	1.269GluAsn: 1.269 ± 0.031	2.737GluPro: 2.737 ± 0.045	2.855GluGln: 2.855 ± 0.053	5.126GluArg: 5.126 ± 0.072	3.498GluSer: 3.498 ± 0.059	3.205GluThr: 3.205 ± 0.052	3.963GluVal: 3.963 ± 0.063	0.863GluTrp: 0.863 ± 0.028	1.421GluTyr: 1.421 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	3.254PheAla: 3.254 ± 0.052	0.426PheCys: 0.426 ± 0.019	2.326PheAsp: 2.326 ± 0.041	2.105PheGlu: 2.105 ± 0.039	1.461PhePhe: 1.461 ± 0.038	3.23PheGly: 3.23 ± 0.058	0.988PheHis: 0.988 ± 0.03	1.591PheIle: 1.591 ± 0.041	1.209PheLys: 1.209 ± 0.035	3.815PheLeu: 3.815 ± 0.071	0.822PheMet: 0.822 ± 0.028	1.138PheAsn: 1.138 ± 0.036	1.851PhePro: 1.851 ± 0.04	1.253PheGln: 1.253 ± 0.035	2.462PheArg: 2.462 ± 0.04	2.459PheSer: 2.459 ± 0.051	2.107PheThr: 2.107 ± 0.041	2.791PheVal: 2.791 ± 0.044	0.521PheTrp: 0.521 ± 0.017	1.039PheTyr: 1.039 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	6.981GlyAla: 6.981 ± 0.119	1.004GlyCys: 1.004 ± 0.031	3.789GlyAsp: 3.789 ± 0.052	4.366GlyGlu: 4.366 ± 0.066	3.187GlyPhe: 3.187 ± 0.056	6.369GlyGly: 6.369 ± 0.083	1.933GlyHis: 1.933 ± 0.045	4.483GlyIle: 4.483 ± 0.058	3.558GlyLys: 3.558 ± 0.066	8.38GlyLeu: 8.38 ± 0.091	2.136GlyMet: 2.136 ± 0.046	2.263GlyAsn: 2.263 ± 0.068	3.273GlyPro: 3.273 ± 0.056	2.985GlyGln: 2.985 ± 0.058	5.72GlyArg: 5.72 ± 0.084	4.836GlySer: 4.836 ± 0.076	5.16GlyThr: 5.16 ± 0.086	5.751GlyVal: 5.751 ± 0.068	1.274GlyTrp: 1.274 ± 0.034	2.285GlyTyr: 2.285 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.253HisAla: 2.253 ± 0.039	0.312HisCys: 0.312 ± 0.017	1.321HisAsp: 1.321 ± 0.032	1.421HisGlu: 1.421 ± 0.032	0.867HisPhe: 0.867 ± 0.025	1.95HisGly: 1.95 ± 0.049	0.754HisHis: 0.754 ± 0.023	1.024HisIle: 1.024 ± 0.028	0.636HisLys: 0.636 ± 0.022	2.448HisLeu: 2.448 ± 0.048	0.527HisMet: 0.527 ± 0.022	0.564HisAsn: 0.564 ± 0.021	1.637HisPro: 1.637 ± 0.04	0.889HisGln: 0.889 ± 0.024	1.905HisArg: 1.905 ± 0.049	1.222HisSer: 1.222 ± 0.031	1.079HisThr: 1.079 ± 0.032	1.821HisVal: 1.821 ± 0.044	0.342HisTrp: 0.342 ± 0.015	0.648HisTyr: 0.648 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.09IleAla: 5.09 ± 0.072	0.499IleCys: 0.499 ± 0.023	3.073IleAsp: 3.073 ± 0.055	3.449IleGlu: 3.449 ± 0.06	1.589IlePhe: 1.589 ± 0.039	4.545IleGly: 4.545 ± 0.078	1.197IleHis: 1.197 ± 0.029	2.159IleIle: 2.159 ± 0.047	1.881IleLys: 1.881 ± 0.042	4.825IleLeu: 4.825 ± 0.068	0.922IleMet: 0.922 ± 0.032	1.383IleAsn: 1.383 ± 0.037	2.923IlePro: 2.923 ± 0.048	1.761IleGln: 1.761 ± 0.038	3.667IleArg: 3.667 ± 0.059	2.687IleSer: 2.687 ± 0.05	2.709IleThr: 2.709 ± 0.079	4.176IleVal: 4.176 ± 0.064	0.543IleTrp: 0.543 ± 0.025	1.134IleTyr: 1.134 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	3.812LysAla: 3.812 ± 0.066	0.242LysCys: 0.242 ± 0.015	2.269LysAsp: 2.269 ± 0.053	2.592LysGlu: 2.592 ± 0.056	1.076LysPhe: 1.076 ± 0.034	2.999LysGly: 2.999 ± 0.064	0.868LysHis: 0.868 ± 0.024	1.97LysIle: 1.97 ± 0.046	1.923LysLys: 1.923 ± 0.055	3.487LysLeu: 3.487 ± 0.057	0.933LysMet: 0.933 ± 0.029	1.082LysAsn: 1.082 ± 0.035	2.181LysPro: 2.181 ± 0.049	1.714LysGln: 1.714 ± 0.044	2.766LysArg: 2.766 ± 0.046	2.188LysSer: 2.188 ± 0.051	2.434LysThr: 2.434 ± 0.044	2.718LysVal: 2.718 ± 0.057	0.437LysTrp: 0.437 ± 0.021	0.818LysTyr: 0.818 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	10.941LeuAla: 10.941 ± 0.12	1.165LeuCys: 1.165 ± 0.031	5.582LeuAsp: 5.582 ± 0.066	5.697LeuGlu: 5.697 ± 0.083	3.915LeuPhe: 3.915 ± 0.071	7.592LeuGly: 7.592 ± 0.094	2.307LeuHis: 2.307 ± 0.049	5.186LeuIle: 5.186 ± 0.083	4.179LeuLys: 4.179 ± 0.055	10.836LeuLeu: 10.836 ± 0.137	2.286LeuMet: 2.286 ± 0.049	2.977LeuAsn: 2.977 ± 0.059	5.515LeuPro: 5.515 ± 0.074	3.615LeuGln: 3.615 ± 0.051	7.321LeuArg: 7.321 ± 0.088	6.986LeuSer: 6.986 ± 0.081	6.45LeuThr: 6.45 ± 0.115	7.518LeuVal: 7.518 ± 0.089	1.219LeuTrp: 1.219 ± 0.034	2.644LeuTyr: 2.644 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	2.514MetAla: 2.514 ± 0.046	0.173MetCys: 0.173 ± 0.012	1.242MetAsp: 1.242 ± 0.03	1.284MetGlu: 1.284 ± 0.035	0.652MetPhe: 0.652 ± 0.024	1.769MetGly: 1.769 ± 0.043	0.458MetHis: 0.458 ± 0.02	1.229MetIle: 1.229 ± 0.033	1.301MetLys: 1.301 ± 0.042	2.16MetLeu: 2.16 ± 0.046	0.605MetMet: 0.605 ± 0.023	0.831MetAsn: 0.831 ± 0.026	1.46MetPro: 1.46 ± 0.035	0.843MetGln: 0.843 ± 0.027	1.634MetArg: 1.634 ± 0.032	1.487MetSer: 1.487 ± 0.034	1.734MetThr: 1.734 ± 0.037	1.747MetVal: 1.747 ± 0.038	0.229MetTrp: 0.229 ± 0.013	0.422MetTyr: 0.422 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.449AsnAla: 2.449 ± 0.072	0.257AsnCys: 0.257 ± 0.013	1.361AsnAsp: 1.361 ± 0.043	1.351AsnGlu: 1.351 ± 0.037	1.001AsnPhe: 1.001 ± 0.035	2.379AsnGly: 2.379 ± 0.057	0.633AsnHis: 0.633 ± 0.021	1.32AsnIle: 1.32 ± 0.039	0.866AsnLys: 0.866 ± 0.033	2.87AsnLeu: 2.87 ± 0.06	0.578AsnMet: 0.578 ± 0.022	0.831AsnAsn: 0.831 ± 0.074	1.93AsnPro: 1.93 ± 0.043	1.109AsnGln: 1.109 ± 0.033	2.06AsnArg: 2.06 ± 0.044	1.379AsnSer: 1.379 ± 0.037	1.291AsnThr: 1.291 ± 0.037	2.086AsnVal: 2.086 ± 0.063	0.375AsnTrp: 0.375 ± 0.019	0.729AsnTyr: 0.729 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	5.422ProAla: 5.422 ± 0.068	0.45ProCys: 0.45 ± 0.02	3.425ProAsp: 3.425 ± 0.056	3.273ProGlu: 3.273 ± 0.048	2.045ProPhe: 2.045 ± 0.04	4.161ProGly: 4.161 ± 0.059	1.204ProHis: 1.204 ± 0.033	2.485ProIle: 2.485 ± 0.048	1.865ProLys: 1.865 ± 0.046	5.046ProLeu: 5.046 ± 0.068	1.258ProMet: 1.258 ± 0.031	1.465ProAsn: 1.465 ± 0.035	2.968ProPro: 2.968 ± 0.06	1.816ProGln: 1.816 ± 0.038	2.883ProArg: 2.883 ± 0.052	3.718ProSer: 3.718 ± 0.061	2.846ProThr: 2.846 ± 0.053	4.411ProVal: 4.411 ± 0.065	0.725ProTrp: 0.725 ± 0.025	1.319ProTyr: 1.319 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	4.311GlnAla: 4.311 ± 0.066	0.308GlnCys: 0.308 ± 0.016	1.929GlnAsp: 1.929 ± 0.034	2.338GlnGlu: 2.338 ± 0.049	1.395GlnPhe: 1.395 ± 0.035	2.795GlnGly: 2.795 ± 0.048	0.879GlnHis: 0.879 ± 0.031	1.899GlnIle: 1.899 ± 0.049	1.345GlnLys: 1.345 ± 0.037	3.65GlnLeu: 3.65 ± 0.055	0.845GlnMet: 0.845 ± 0.025	0.899GlnAsn: 0.899 ± 0.029	2.124GlnPro: 2.124 ± 0.043	1.848GlnGln: 1.848 ± 0.048	2.766GlnArg: 2.766 ± 0.052	2.314GlnSer: 2.314 ± 0.046	2.07GlnThr: 2.07 ± 0.046	2.835GlnVal: 2.835 ± 0.043	0.63GlnTrp: 0.63 ± 0.019	1.0GlnTyr: 1.0 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	6.258ArgAla: 6.258 ± 0.077	0.723ArgCys: 0.723 ± 0.027	3.636ArgAsp: 3.636 ± 0.049	4.444ArgGlu: 4.444 ± 0.061	2.903ArgPhe: 2.903 ± 0.053	4.481ArgGly: 4.481 ± 0.066	1.982ArgHis: 1.982 ± 0.047	4.099ArgIle: 4.099 ± 0.067	2.531ArgLys: 2.531 ± 0.055	8.339ArgLeu: 8.339 ± 0.099	2.032ArgMet: 2.032 ± 0.046	1.933ArgAsn: 1.933 ± 0.047	3.475ArgPro: 3.475 ± 0.061	3.031ArgGln: 3.031 ± 0.05	5.878ArgArg: 5.878 ± 0.088	4.236ArgSer: 4.236 ± 0.06	4.026ArgThr: 4.026 ± 0.062	4.962ArgVal: 4.962 ± 0.067	1.24ArgTrp: 1.24 ± 0.031	2.218ArgTyr: 2.218 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	5.523SerAla: 5.523 ± 0.079	0.616SerCys: 0.616 ± 0.021	3.134SerAsp: 3.134 ± 0.049	3.17SerGlu: 3.17 ± 0.051	2.434SerPhe: 2.434 ± 0.043	5.53SerGly: 5.53 ± 0.09	1.442SerHis: 1.442 ± 0.039	2.94SerIle: 2.94 ± 0.045	2.078SerLys: 2.078 ± 0.046	6.493SerLeu: 6.493 ± 0.094	1.569SerMet: 1.569 ± 0.031	1.563SerAsn: 1.563 ± 0.04	3.346SerPro: 3.346 ± 0.057	2.184SerGln: 2.184 ± 0.042	4.222SerArg: 4.222 ± 0.058	3.876SerSer: 3.876 ± 0.068	3.305SerThr: 3.305 ± 0.071	4.527SerVal: 4.527 ± 0.064	0.889SerTrp: 0.889 ± 0.033	1.543SerTyr: 1.543 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	5.505ThrAla: 5.505 ± 0.08	0.49ThrCys: 0.49 ± 0.019	2.941ThrAsp: 2.941 ± 0.049	2.901ThrGlu: 2.901 ± 0.047	2.194ThrPhe: 2.194 ± 0.047	5.196ThrGly: 5.196 ± 0.082	1.199ThrHis: 1.199 ± 0.031	3.216ThrIle: 3.216 ± 0.062	2.064ThrLys: 2.064 ± 0.041	5.927ThrLeu: 5.927 ± 0.132	1.272ThrMet: 1.272 ± 0.034	1.545ThrAsn: 1.545 ± 0.067	3.251ThrPro: 3.251 ± 0.052	1.809ThrGln: 1.809 ± 0.048	3.118ThrArg: 3.118 ± 0.059	3.17ThrSer: 3.17 ± 0.052	3.313ThrThr: 3.313 ± 0.1	5.143ThrVal: 5.143 ± 0.081	0.773ThrTrp: 0.773 ± 0.033	1.403ThrTyr: 1.403 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	7.324ValAla: 7.324 ± 0.09	0.759ValCys: 0.759 ± 0.027	3.868ValAsp: 3.868 ± 0.059	4.637ValGlu: 4.637 ± 0.06	2.76ValPhe: 2.76 ± 0.054	5.671ValGly: 5.671 ± 0.073	1.582ValHis: 1.582 ± 0.038	3.951ValIle: 3.951 ± 0.06	3.114ValLys: 3.114 ± 0.061	8.017ValLeu: 8.017 ± 0.095	1.821ValMet: 1.821 ± 0.043	2.098ValAsn: 2.098 ± 0.043	3.996ValPro: 3.996 ± 0.054	2.65ValGln: 2.65 ± 0.05	5.625ValArg: 5.625 ± 0.074	4.931ValSer: 4.931 ± 0.062	4.892ValThr: 4.892 ± 0.086	6.368ValVal: 6.368 ± 0.078	0.915ValTrp: 0.915 ± 0.033	1.707ValTyr: 1.707 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.087TrpAla: 1.087 ± 0.032	0.168TrpCys: 0.168 ± 0.01	0.655TrpAsp: 0.655 ± 0.024	0.806TrpGlu: 0.806 ± 0.027	0.581TrpPhe: 0.581 ± 0.022	0.896TrpGly: 0.896 ± 0.033	0.358TrpHis: 0.358 ± 0.017	0.764TrpIle: 0.764 ± 0.023	0.68TrpLys: 0.68 ± 0.029	1.643TrpLeu: 1.643 ± 0.042	0.399TrpMet: 0.399 ± 0.021	0.505TrpAsn: 0.505 ± 0.021	0.594TrpPro: 0.594 ± 0.022	0.541TrpGln: 0.541 ± 0.02	0.961TrpArg: 0.961 ± 0.033	0.908TrpSer: 0.908 ± 0.032	0.847TrpThr: 0.847 ± 0.025	0.922TrpVal: 0.922 ± 0.029	0.29TrpTrp: 0.29 ± 0.017	0.398TrpTyr: 0.398 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.229TyrAla: 2.229 ± 0.047	0.298TyrCys: 0.298 ± 0.017	1.493TyrAsp: 1.493 ± 0.039	1.512TyrGlu: 1.512 ± 0.036	1.128TyrPhe: 1.128 ± 0.035	2.231TyrGly: 2.231 ± 0.042	0.673TyrHis: 0.673 ± 0.026	0.947TyrIle: 0.947 ± 0.028	0.806TyrLys: 0.806 ± 0.03	2.757TyrLeu: 2.757 ± 0.052	0.474TyrMet: 0.474 ± 0.022	0.723TyrAsn: 0.723 ± 0.027	1.274TyrPro: 1.274 ± 0.032	1.084TyrGln: 1.084 ± 0.03	2.404TyrArg: 2.404 ± 0.043	1.362TyrSer: 1.362 ± 0.038	1.152TyrThr: 1.152 ± 0.029	1.893TyrVal: 1.893 ± 0.04	0.447TyrTrp: 0.447 ± 0.019	0.88TyrTyr: 0.88 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4147 proteins (1241337 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---