Apibacter adventoris
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2420 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S8ACM7|A0A2S8ACM7_9FLAO Nuclease SbcCD subunit D OS=Apibacter adventoris OX=1679466 GN=sbcD PE=3 SV=1
MM1 pKa = 7.78 AIKK4 pKa = 9.16 ITDD7 pKa = 3.28 EE8 pKa = 4.77 CINCGACEE16 pKa = 4.03 PEE18 pKa = 4.51 CPNNAIYY25 pKa = 10.44 EE26 pKa = 4.38 GAVDD30 pKa = 3.55 WKK32 pKa = 9.98 MSEE35 pKa = 4.08 GTEE38 pKa = 3.97 LTGMIVTKK46 pKa = 10.14 TGLSIDD52 pKa = 3.64 ADD54 pKa = 3.97 APQKK58 pKa = 10.38 PLSDD62 pKa = 4.84 DD63 pKa = 2.75 IYY65 pKa = 11.34 YY66 pKa = 10.02 IVTDD70 pKa = 3.63 KK71 pKa = 10.8 CTEE74 pKa = 3.87 CEE76 pKa = 4.36 GFHH79 pKa = 7.15 EE80 pKa = 5.02 EE81 pKa = 4.11 PQCAAVCPVDD91 pKa = 4.51 CCVPDD96 pKa = 4.45 DD97 pKa = 3.94 NHH99 pKa = 7.13 VEE101 pKa = 4.1 TSEE104 pKa = 3.94 EE105 pKa = 3.86 LLAKK109 pKa = 10.5 KK110 pKa = 10.44 NFLHH114 pKa = 6.95 SSSS117 pKa = 3.56
Molecular weight: 12.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.983
IPC2_protein 4.126
IPC_protein 4.05
Toseland 3.872
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.91
Rodwell 3.884
Grimsley 3.783
Solomon 4.012
Lehninger 3.961
Nozaki 4.126
DTASelect 4.291
Thurlkill 3.91
EMBOSS 3.923
Sillero 4.164
Patrickios 0.337
IPC_peptide 4.012
IPC2_peptide 4.151
IPC2.peptide.svr19 4.055
Protein with the highest isoelectric point:
>tr|A0A2S8AGS1|A0A2S8AGS1_9FLAO Uncharacterized protein OS=Apibacter adventoris OX=1679466 GN=C4S77_01955 PE=4 SV=1
MM1 pKa = 7.5 AKK3 pKa = 10.25 EE4 pKa = 3.94 SMKK7 pKa = 10.47 ARR9 pKa = 11.84 EE10 pKa = 4.26 RR11 pKa = 11.84 KK12 pKa = 9.55 RR13 pKa = 11.84 EE14 pKa = 3.95 ALAAKK19 pKa = 9.76 YY20 pKa = 9.22 AAKK23 pKa = 10.34 RR24 pKa = 11.84 KK25 pKa = 9.11 ALKK28 pKa = 10.1 EE29 pKa = 4.16 AGDD32 pKa = 3.85 YY33 pKa = 8.4 EE34 pKa = 4.6 ALQKK38 pKa = 10.73 LPKK41 pKa = 9.9 NSSPVRR47 pKa = 11.84 LHH49 pKa = 6.16 NRR51 pKa = 11.84 CKK53 pKa = 9.95 LTGRR57 pKa = 11.84 PRR59 pKa = 11.84 GYY61 pKa = 9.31 MRR63 pKa = 11.84 VFGISRR69 pKa = 11.84 VTFRR73 pKa = 11.84 EE74 pKa = 3.86 MANEE78 pKa = 3.77 GLIPGVKK85 pKa = 9.29 KK86 pKa = 10.96 ASWW89 pKa = 3.03
Molecular weight: 10.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.853
IPC_protein 10.511
Toseland 10.965
ProMoST 10.584
Dawson 11.023
Bjellqvist 10.701
Wikipedia 11.213
Rodwell 11.403
Grimsley 11.052
Solomon 11.155
Lehninger 11.125
Nozaki 10.935
DTASelect 10.701
Thurlkill 10.95
EMBOSS 11.359
Sillero 10.965
Patrickios 11.125
IPC_peptide 11.155
IPC2_peptide 9.458
IPC2.peptide.svr19 8.588
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2420
0
2420
786421
38
3284
325.0
37.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.965 ± 0.049
0.856 ± 0.017
5.025 ± 0.034
6.681 ± 0.056
5.254 ± 0.048
6.004 ± 0.049
1.643 ± 0.021
9.605 ± 0.061
9.213 ± 0.047
9.335 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.031 ± 0.02
6.7 ± 0.05
3.347 ± 0.026
3.453 ± 0.029
3.04 ± 0.033
6.67 ± 0.039
5.169 ± 0.033
5.199 ± 0.039
1.0 ± 0.019
4.812 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here