Brevinema andersonii

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Brevinematales; Brevinemataceae; Brevinema

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1441 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1DHX4|A0A1I1DHX4_9SPIR Predicted permease YjgP/YjgQ family protein OS=Brevinema andersonii OX=34097 GN=SAMN02745150_00511 PE=4 SV=1
MM1 pKa = 7.89LEE3 pKa = 4.14GTNDD7 pKa = 3.11VYY9 pKa = 11.67GEE11 pKa = 3.86IMAIQGNYY19 pKa = 9.7EE20 pKa = 4.49EE21 pKa = 4.77ILKK24 pKa = 10.44ILDD27 pKa = 4.31DD28 pKa = 4.43LEE30 pKa = 4.79CFYY33 pKa = 11.68GDD35 pKa = 4.23GIISNEE41 pKa = 3.84YY42 pKa = 9.78DD43 pKa = 3.18RR44 pKa = 11.84KK45 pKa = 10.63KK46 pKa = 11.05VDD48 pKa = 3.34VLNIDD53 pKa = 3.67TDD55 pKa = 3.67QKK57 pKa = 10.69EE58 pKa = 4.03QLSVYY63 pKa = 10.62FLMM66 pKa = 5.83

Molecular weight: 7.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1E0L6|A0A1I1E0L6_9SPIR Uncharacterized protein OS=Brevinema andersonii OX=34097 GN=SAMN02745150_00863 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 9.43RR3 pKa = 11.84TWHH6 pKa = 6.0PSKK9 pKa = 10.31IKK11 pKa = 10.18RR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 8.66HH16 pKa = 4.7GFRR19 pKa = 11.84AKK21 pKa = 9.73MADD24 pKa = 2.99RR25 pKa = 11.84HH26 pKa = 5.23GRR28 pKa = 11.84AILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.57GRR39 pKa = 11.84VRR41 pKa = 11.84LTVV44 pKa = 2.92

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1441

0

1441

437839

40

2974

303.8

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.559 ± 0.066

0.829 ± 0.022

5.016 ± 0.045

6.296 ± 0.07

5.383 ± 0.06

5.85 ± 0.065

1.94 ± 0.029

8.919 ± 0.082

6.778 ± 0.061

10.279 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.416 ± 0.028

5.388 ± 0.065

3.782 ± 0.035

3.982 ± 0.046

4.051 ± 0.045

6.731 ± 0.044

5.245 ± 0.058

5.712 ± 0.061

0.97 ± 0.024

3.874 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski