Bacilladnavirus sp.
Average proteome isoelectric point is 7.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345N1F1|A0A345N1F1_9VIRU Uncharacterized protein OS=Bacilladnavirus sp. OX=2267680 PE=4 SV=1
MM1 pKa = 7.09 QHH3 pKa = 6.32 LQFFAQTHH11 pKa = 6.67 LYY13 pKa = 9.86 CSPATFLHH21 pKa = 6.61 SGVQFPWRR29 pKa = 11.84 STIPFGTTAKK39 pKa = 10.14 QSRR42 pKa = 11.84 ADD44 pKa = 3.52 MTRR47 pKa = 11.84 CCCCPNNEE55 pKa = 4.09 QTTGYY60 pKa = 10.26 APPAFNPSPTGPNVPGIGDD79 pKa = 4.26 DD80 pKa = 3.39 IFGEE84 pKa = 4.14 LHH86 pKa = 6.74 SGIPEE91 pKa = 3.95 NGLFPEE97 pKa = 5.0 APCVKK102 pKa = 10.69 NEE104 pKa = 4.04 DD105 pKa = 4.23 AYY107 pKa = 11.16 DD108 pKa = 3.88 DD109 pKa = 4.9 EE110 pKa = 5.79 INEE113 pKa = 4.56 GGDD116 pKa = 3.45 PSTAPVPPPVFTTQYY131 pKa = 9.84 SPSFSAISGKK141 pKa = 10.63 SSGDD145 pKa = 3.56 CIQANTALYY154 pKa = 10.26 ARR156 pKa = 11.84 HH157 pKa = 6.73 DD158 pKa = 3.93 PDD160 pKa = 5.64 GISGVTWSTWDD171 pKa = 3.74 GITRR175 pKa = 11.84 FNPCALTKK183 pKa = 10.22 AQLYY187 pKa = 8.87 AAIWPTGDD195 pKa = 3.52 CRR197 pKa = 11.84 NMLGLRR203 pKa = 11.84 DD204 pKa = 4.51 LYY206 pKa = 11.58 YY207 pKa = 10.61 SINPFADD214 pKa = 3.2 VANPTIAEE222 pKa = 3.64 IDD224 pKa = 3.14 AWHH227 pKa = 6.98 AIVVNHH233 pKa = 5.7 YY234 pKa = 10.58 RR235 pKa = 11.84 DD236 pKa = 3.72 LLGIATPILNDD247 pKa = 2.97 ANLYY251 pKa = 9.87 VRR253 pKa = 11.84 AQWASEE259 pKa = 4.04 RR260 pKa = 11.84 KK261 pKa = 8.06 NTTYY265 pKa = 10.79 WDD267 pKa = 3.33 TSYY270 pKa = 8.47 PTGEE274 pKa = 4.15 LCPTGSSTHH283 pKa = 6.47 CGWTFIPSCLDD294 pKa = 3.14 QAPYY298 pKa = 10.07 LAPGQGCVASTPQSEE313 pKa = 5.25 GIFGALPNPWSVRR326 pKa = 11.84 FACVLAKK333 pKa = 10.11 IVQTEE338 pKa = 4.88 GITGHH343 pKa = 6.77 GGPFLGRR350 pKa = 11.84 PNMGMNFWCNGSEE363 pKa = 3.82 TSLRR367 pKa = 11.84 IKK369 pKa = 10.84 FNGVPVNPCPPVV381 pKa = 3.07
Molecular weight: 41.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.026
IPC2_protein 4.978
IPC_protein 4.94
Toseland 4.902
ProMoST 5.118
Dawson 4.978
Bjellqvist 5.092
Wikipedia 4.876
Rodwell 4.876
Grimsley 4.838
Solomon 4.965
Lehninger 4.927
Nozaki 5.105
DTASelect 5.308
Thurlkill 4.94
EMBOSS 4.94
Sillero 5.169
Patrickios 0.579
IPC_peptide 4.978
IPC2_peptide 5.169
IPC2.peptide.svr19 4.994
Protein with the highest isoelectric point:
>tr|A0A345N1E7|A0A345N1E7_9VIRU Uncharacterized protein OS=Bacilladnavirus sp. OX=2267680 PE=4 SV=1
MM1 pKa = 7.98 KK2 pKa = 10.34 IFDD5 pKa = 5.12 DD6 pKa = 6.21 DD7 pKa = 3.63 NDD9 pKa = 3.28 RR10 pKa = 11.84 WITIGRR16 pKa = 11.84 KK17 pKa = 8.47 PRR19 pKa = 11.84 YY20 pKa = 9.02 NPRR23 pKa = 11.84 TIANDD28 pKa = 3.86 RR29 pKa = 11.84 QWCSYY34 pKa = 9.78 HH35 pKa = 7.22 RR36 pKa = 11.84 LPEE39 pKa = 3.83 YY40 pKa = 10.51 RR41 pKa = 11.84 GLKK44 pKa = 9.77 GFKK47 pKa = 9.67 KK48 pKa = 10.3 GLKK51 pKa = 9.32 ARR53 pKa = 11.84 QEE55 pKa = 4.0 IFTTFQKK62 pKa = 10.37 KK63 pKa = 10.2 CSVALRR69 pKa = 11.84 KK70 pKa = 10.09 VYY72 pKa = 9.11 EE73 pKa = 4.37 AKK75 pKa = 10.4 RR76 pKa = 11.84 SMRR79 pKa = 11.84 RR80 pKa = 11.84 LLMNRR85 pKa = 11.84 DD86 pKa = 3.88 KK87 pKa = 11.52 VCLDD91 pKa = 3.39 ASIKK95 pKa = 9.58 KK96 pKa = 9.54 AAYY99 pKa = 9.25 RR100 pKa = 11.84 DD101 pKa = 3.6 MYY103 pKa = 9.66 RR104 pKa = 11.84 WKK106 pKa = 10.53 AIKK109 pKa = 9.62 QDD111 pKa = 3.85 RR112 pKa = 11.84 LRR114 pKa = 11.84 QAAKK118 pKa = 10.17 LNAFRR123 pKa = 11.84 RR124 pKa = 11.84 SDD126 pKa = 3.47 PAGYY130 pKa = 10.99 AFMKK134 pKa = 10.66 SFTPKK139 pKa = 10.09 KK140 pKa = 10.04 KK141 pKa = 9.92 KK142 pKa = 10.26 CKK144 pKa = 10.1 CSS146 pKa = 3.21
Molecular weight: 17.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.306
IPC2_protein 9.78
IPC_protein 10.175
Toseland 10.73
ProMoST 10.306
Dawson 10.818
Bjellqvist 10.467
Wikipedia 10.979
Rodwell 11.286
Grimsley 10.862
Solomon 10.891
Lehninger 10.862
Nozaki 10.716
DTASelect 10.452
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.994
IPC_peptide 10.891
IPC2_peptide 9.326
IPC2.peptide.svr19 8.603
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
2022
84
404
224.7
25.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.578 ± 0.595
2.671 ± 0.778
5.885 ± 0.835
4.649 ± 0.602
4.303 ± 0.631
5.786 ± 0.882
2.226 ± 0.477
5.045 ± 0.496
6.33 ± 1.274
7.616 ± 0.968
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.978 ± 0.28
4.055 ± 0.686
8.111 ± 1.202
3.808 ± 0.198
6.38 ± 1.081
7.567 ± 0.966
6.034 ± 0.753
5.589 ± 0.925
1.632 ± 0.341
3.759 ± 0.469
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here