Wuhan japanese halfbeak arterivirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Nanidovirineae; Nanhypoviridae; Hyporhamsavirinae; Sajorinivirus; Halfbeak nidovirus 1

Average proteome isoelectric point is 7.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1GMX5|A0A2P1GMX5_9NIDO Uncharacterized protein OS=Wuhan japanese halfbeak arterivirus OX=2116443 PE=4 SV=1
MM1 pKa = 7.93LSGFWLLLLPSLLLVHH17 pKa = 6.79FFLSCALCLGFSLCWSTSS35 pKa = 2.95

Molecular weight: 3.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1GMU2|A0A2P1GMU2_9NIDO Non-structural protein 8 OS=Wuhan japanese halfbeak arterivirus OX=2116443 PE=4 SV=1
MM1 pKa = 8.09DD2 pKa = 5.34PRR4 pKa = 11.84ASPYY8 pKa = 10.48SPSGQSQAPVVYY20 pKa = 9.92GAPPPWWFRR29 pKa = 11.84GPPRR33 pKa = 11.84RR34 pKa = 11.84NNGNGGRR41 pKa = 11.84GNNGGGNSEE50 pKa = 4.19MKK52 pKa = 10.72KK53 pKa = 10.48LVTAVAALTKK63 pKa = 10.21AQSKK67 pKa = 5.1TTGGAGKK74 pKa = 9.74KK75 pKa = 6.7QTPSGKK81 pKa = 9.32PPQKK85 pKa = 10.26AVKK88 pKa = 9.23EE89 pKa = 4.07FPGYY93 pKa = 10.57HH94 pKa = 5.72FFGGKK99 pKa = 9.02YY100 pKa = 9.17YY101 pKa = 10.79KK102 pKa = 10.74NSDD105 pKa = 3.08GHH107 pKa = 6.21SGKK110 pKa = 10.16DD111 pKa = 3.19LSEE114 pKa = 4.36AFSKK118 pKa = 11.41LEE120 pKa = 3.8IAKK123 pKa = 10.49DD124 pKa = 3.61DD125 pKa = 3.71

Molecular weight: 13.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

5434

35

2864

1086.8

119.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.643 ± 0.725

3.239 ± 0.318

4.656 ± 0.549

3.662 ± 0.225

4.785 ± 0.42

7.14 ± 0.993

2.282 ± 0.069

4.085 ± 0.528

4.49 ± 0.815

9.79 ± 0.91

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.822 ± 0.085

3.11 ± 0.239

6.919 ± 0.407

2.926 ± 0.429

4.472 ± 0.295

9.22 ± 0.676

7.563 ± 0.729

8.318 ± 0.911

1.564 ± 0.398

3.312 ± 0.36
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski