Proteome-pI 2.0 - Proteome Isoelectric Point Database

Arthrobacter phage StevieBAY

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Marthavirus; unclassified Marthavirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A6M3T554|A0A6M3T554_9CAUD Uncharacterized protein OS=Arthrobacter phage StevieBAY OX=2725609 GN=53 PE=4 SV=1

MM1 pKa = 7.23TEE3 pKa = 3.67VRR5 pKa = 11.84HH6 pKa = 6.79SEE8 pKa = 4.03FTDD11 pKa = 3.58SEE13 pKa = 4.43GQTFIIEE20 pKa = 4.31SEE22 pKa = 4.32DD23 pKa = 3.58GVISIQTYY31 pKa = 9.69GGHH34 pKa = 6.6IDD36 pKa = 4.45EE37 pKa = 5.38LDD39 pKa = 3.5EE40 pKa = 4.72GNCAGCEE47 pKa = 3.94DD48 pKa = 4.97CIEE51 pKa = 4.29TVALFAVDD59 pKa = 3.4APKK62 pKa = 10.3IIVALQRR69 pKa = 11.84ALQHH73 pKa = 6.27AATTGEE79 pKa = 4.15YY80 pKa = 10.95GRR82 pKa = 11.84GEE84 pKa = 4.55TISGQCVDD92 pKa = 4.57VNSDD96 pKa = 3.63DD97 pKa = 3.9PFNAVSQLQSYY108 pKa = 9.69DD109 pKa = 4.02EE110 pKa = 5.15PDD112 pKa = 3.36MSPDD116 pKa = 3.23SPHH119 pKa = 6.22YY120 pKa = 10.12GKK122 pKa = 9.34PHH124 pKa = 6.77EE125 pKa = 5.15DD126 pKa = 3.3CCKK129 pKa = 10.59

Molecular weight: 14.0 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A6M3T526|A0A6M3T526_9CAUD Minor tail protein OS=Arthrobacter phage StevieBAY OX=2725609 GN=23 PE=4 SV=1

MM1 pKa = 7.03TRR3 pKa = 11.84EE4 pKa = 3.86GLYY7 pKa = 10.5VRR9 pKa = 11.84GVEE12 pKa = 4.11HH13 pKa = 6.67SAAKK17 pKa = 10.49LRR19 pKa = 11.84DD20 pKa = 3.43ADD22 pKa = 3.87VVRR25 pKa = 11.84MRR27 pKa = 11.84EE28 pKa = 3.52IAAGEE33 pKa = 4.23RR34 pKa = 11.84ITLRR38 pKa = 11.84EE39 pKa = 3.6LATRR43 pKa = 11.84FGVTTTAVHH52 pKa = 7.07RR53 pKa = 11.84IITGAGWAHH62 pKa = 6.84LDD64 pKa = 3.63GPIRR68 pKa = 11.84EE69 pKa = 4.2PRR71 pKa = 11.84SYY73 pKa = 10.69TKK75 pKa = 10.59RR76 pKa = 11.84KK77 pKa = 9.34

Molecular weight: 8.67 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
79	0	79	16160	44	877	204.6	22.35

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
11.429 ± 0.542	0.662 ± 0.105	6.361 ± 0.262	6.423 ± 0.39	2.964 ± 0.135	7.376 ± 0.436	1.844 ± 0.168	4.926 ± 0.244	4.684 ± 0.198	8.032 ± 0.277

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.327 ± 0.138	3.614 ± 0.199	4.814 ± 0.242	3.824 ± 0.2	6.132 ± 0.399	6.095 ± 0.304	6.788 ± 0.389	7.748 ± 0.295	1.621 ± 0.146	2.333 ± 0.13

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski