Thermocrinis albus (strain DSM 14484 / JCM 11386 / HI 11/12)
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1592 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D3SPK9|D3SPK9_THEAH Flavocytochrome c sulphide dehydrogenase flavin-binding protein OS=Thermocrinis albus (strain DSM 14484 / JCM 11386 / HI 11/12) OX=638303 GN=Thal_0462 PE=4 SV=1
MM1 pKa = 7.88 PYY3 pKa = 10.16 SIKK6 pKa = 10.31 KK7 pKa = 9.57 DD8 pKa = 3.55 LCISCYY14 pKa = 10.46 ACEE17 pKa = 4.29 DD18 pKa = 3.47 QCPTKK23 pKa = 10.54 AISHH27 pKa = 5.61 NEE29 pKa = 3.66 EE30 pKa = 3.99 GEE32 pKa = 4.14 FFIDD36 pKa = 3.2 PTVCVEE42 pKa = 4.18 CEE44 pKa = 3.89 GFYY47 pKa = 11.0 EE48 pKa = 4.7 EE49 pKa = 4.65 PQCLAICPIDD59 pKa = 3.61 GCIVKK64 pKa = 10.44 LSEE67 pKa = 4.08 VEE69 pKa = 4.28 EE70 pKa = 4.42 VV71 pKa = 3.14
Molecular weight: 7.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.031
IPC2_protein 4.101
IPC_protein 3.961
Toseland 3.808
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.77
Rodwell 3.795
Grimsley 3.719
Solomon 3.884
Lehninger 3.834
Nozaki 4.037
DTASelect 4.113
Thurlkill 3.834
EMBOSS 3.795
Sillero 4.062
Patrickios 0.006
IPC_peptide 3.897
IPC2_peptide 4.05
IPC2.peptide.svr19 3.98
Protein with the highest isoelectric point:
>tr|D3SM72|D3SM72_THEAH Beta-lactamase domain protein OS=Thermocrinis albus (strain DSM 14484 / JCM 11386 / HI 11/12) OX=638303 GN=Thal_1220 PE=4 SV=1
MM1 pKa = 6.63 ATKK4 pKa = 10.49 RR5 pKa = 11.84 NVTHH9 pKa = 7.2 ISNLKK14 pKa = 9.63 RR15 pKa = 11.84 KK16 pKa = 9.29 RR17 pKa = 11.84 KK18 pKa = 9.7 SGFLARR24 pKa = 11.84 MSSKK28 pKa = 10.36 SGRR31 pKa = 11.84 KK32 pKa = 8.27 IIKK35 pKa = 9.47 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.52 KK40 pKa = 10.18 GRR42 pKa = 11.84 HH43 pKa = 4.83 RR44 pKa = 11.84 LAPP47 pKa = 3.97
Molecular weight: 5.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.515
IPC2_protein 11.228
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.749
Grimsley 13.013
Solomon 13.466
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.486
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.134
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1592
0
1592
479659
30
1566
301.3
34.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.307 ± 0.058
0.94 ± 0.025
4.775 ± 0.035
7.991 ± 0.075
4.377 ± 0.049
7.208 ± 0.056
1.926 ± 0.03
6.174 ± 0.051
6.695 ± 0.058
11.466 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.401 ± 0.027
2.816 ± 0.031
4.36 ± 0.037
2.802 ± 0.036
6.174 ± 0.05
5.367 ± 0.047
4.433 ± 0.032
8.687 ± 0.064
1.131 ± 0.025
3.97 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here