Bovine faeces associated smacovirus 3
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A161IB94|A0A161IB94_9VIRU Putative capsid protein OS=Bovine faeces associated smacovirus 3 OX=1843751 PE=4 SV=1
MM1 pKa = 7.31 EE2 pKa = 5.87 AFRR5 pKa = 11.84 MVSVTLRR12 pKa = 11.84 EE13 pKa = 4.16 TYY15 pKa = 10.57 DD16 pKa = 3.5 LSTKK20 pKa = 8.55 QGKK23 pKa = 6.68 MTLIGIHH30 pKa = 6.54 IPTSNIIQRR39 pKa = 11.84 LYY41 pKa = 10.48 PGFCEE46 pKa = 3.99 QYY48 pKa = 9.9 KK49 pKa = 8.73 MCRR52 pKa = 11.84 INYY55 pKa = 7.55 QNVRR59 pKa = 11.84 IEE61 pKa = 4.26 SASRR65 pKa = 11.84 LPLDD69 pKa = 3.71 PAGVGVNEE77 pKa = 4.54 EE78 pKa = 4.42 GLVSPEE84 pKa = 4.31 DD85 pKa = 3.56 VFNPILYY92 pKa = 9.92 KK93 pKa = 10.64 AVSNQSLSNLEE104 pKa = 3.55 YY105 pKa = 10.48 RR106 pKa = 11.84 IRR108 pKa = 11.84 GLRR111 pKa = 11.84 DD112 pKa = 2.94 QEE114 pKa = 4.25 LSVDD118 pKa = 4.07 GEE120 pKa = 4.47 SATASNEE127 pKa = 3.75 NVTFLDD133 pKa = 3.92 DD134 pKa = 3.93 EE135 pKa = 5.03 FNVYY139 pKa = 10.57 YY140 pKa = 10.7 SILSNTRR147 pKa = 11.84 GWKK150 pKa = 8.69 VAHH153 pKa = 6.23 PQAGLSMRR161 pKa = 11.84 KK162 pKa = 9.14 LRR164 pKa = 11.84 PLVFEE169 pKa = 4.15 KK170 pKa = 10.3 WFSEE174 pKa = 4.24 GVNGVNDD181 pKa = 3.18 FTIPVDD187 pKa = 3.73 SSHH190 pKa = 7.7 LEE192 pKa = 3.91 GEE194 pKa = 4.5 TPVNYY199 pKa = 9.4 IGQMPDD205 pKa = 2.29 EE206 pKa = 4.6 AMRR209 pKa = 11.84 GRR211 pKa = 11.84 PHH213 pKa = 7.84 AMPAFNTKK221 pKa = 10.23 YY222 pKa = 9.76 LTGVQDD228 pKa = 3.5 GSGAGVGKK236 pKa = 9.37 FQQNGMGDD244 pKa = 4.18 GLPGNAQVSMPILPTIYY261 pKa = 9.6 TGLILVPPSRR271 pKa = 11.84 NTTLYY276 pKa = 9.58 YY277 pKa = 10.66 RR278 pKa = 11.84 MVVEE282 pKa = 4.47 TVVSFWGVQTMDD294 pKa = 4.31 EE295 pKa = 4.08 ITSFFNMNNRR305 pKa = 11.84 TRR307 pKa = 11.84 FPVYY311 pKa = 8.85 WNDD314 pKa = 3.22 YY315 pKa = 9.71 RR316 pKa = 11.84 SEE318 pKa = 4.1 NKK320 pKa = 8.61 DD321 pKa = 2.98 TKK323 pKa = 10.57 LTVDD327 pKa = 3.98 EE328 pKa = 4.72 DD329 pKa = 4.65 TVSLSEE335 pKa = 4.19 GADD338 pKa = 3.19 LKK340 pKa = 11.24 KK341 pKa = 10.78 IMEE344 pKa = 4.32
Molecular weight: 38.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.012
IPC2_protein 5.092
IPC_protein 5.016
Toseland 4.927
ProMoST 5.054
Dawson 4.978
Bjellqvist 5.143
Wikipedia 4.838
Rodwell 4.902
Grimsley 4.838
Solomon 4.978
Lehninger 4.94
Nozaki 5.092
DTASelect 5.232
Thurlkill 4.914
EMBOSS 4.876
Sillero 5.169
Patrickios 3.986
IPC_peptide 4.991
IPC2_peptide 5.169
IPC2.peptide.svr19 5.127
Protein with the highest isoelectric point:
>tr|A0A161IB94|A0A161IB94_9VIRU Putative capsid protein OS=Bovine faeces associated smacovirus 3 OX=1843751 PE=4 SV=1
MM1 pKa = 7.5 GKK3 pKa = 10.37 YY4 pKa = 10.17 SVTISADD11 pKa = 2.82 LWKK14 pKa = 10.66 EE15 pKa = 3.49 RR16 pKa = 11.84 DD17 pKa = 3.72 VVRR20 pKa = 11.84 LLDD23 pKa = 3.62 MVDD26 pKa = 3.03 LRR28 pKa = 11.84 EE29 pKa = 4.01 YY30 pKa = 10.88 YY31 pKa = 9.69 IGRR34 pKa = 11.84 EE35 pKa = 3.79 IGKK38 pKa = 9.87 GGYY41 pKa = 8.2 HH42 pKa = 7.3 HH43 pKa = 6.56 YY44 pKa = 10.48 QCAIDD49 pKa = 4.26 CGGDD53 pKa = 3.3 LEE55 pKa = 4.86 RR56 pKa = 11.84 FNGQHH61 pKa = 4.76 QLGWHH66 pKa = 6.5 IEE68 pKa = 4.26 DD69 pKa = 3.85 CASWDD74 pKa = 3.37 KK75 pKa = 11.02 LVRR78 pKa = 11.84 YY79 pKa = 8.65 CRR81 pKa = 11.84 KK82 pKa = 10.24 GGDD85 pKa = 3.21 YY86 pKa = 10.36 RR87 pKa = 11.84 YY88 pKa = 9.89 IGDD91 pKa = 4.1 SIEE94 pKa = 4.09 EE95 pKa = 3.49 QSYY98 pKa = 10.28 RR99 pKa = 11.84 SRR101 pKa = 11.84 GTTAVGHH108 pKa = 6.54 TIDD111 pKa = 3.74 THH113 pKa = 7.07 LKK115 pKa = 8.72 KK116 pKa = 10.89 QNDD119 pKa = 4.0 RR120 pKa = 11.84 QISICVDD127 pKa = 3.27 TKK129 pKa = 11.0 GGSGKK134 pKa = 6.63 TTHH137 pKa = 6.72 GYY139 pKa = 10.11 DD140 pKa = 3.11 RR141 pKa = 11.84 SRR143 pKa = 11.84 TGEE146 pKa = 4.06 YY147 pKa = 10.36 FVVPRR152 pKa = 11.84 NAEE155 pKa = 3.9 TPTRR159 pKa = 11.84 IMDD162 pKa = 3.9 YY163 pKa = 11.27 VAMNYY168 pKa = 10.42 DD169 pKa = 3.94 NEE171 pKa = 4.18 PVIWIDD177 pKa = 3.56 LPRR180 pKa = 11.84 TRR182 pKa = 11.84 KK183 pKa = 8.98 IDD185 pKa = 3.4 KK186 pKa = 10.46 GLATILEE193 pKa = 4.18 DD194 pKa = 3.59 MKK196 pKa = 11.41 DD197 pKa = 3.52 GLIYY201 pKa = 9.39 SAKK204 pKa = 9.78 YY205 pKa = 8.67 EE206 pKa = 4.17 GQVRR210 pKa = 11.84 HH211 pKa = 5.56 IKK213 pKa = 9.54 GVKK216 pKa = 9.55 VLVTTNHH223 pKa = 6.23 YY224 pKa = 9.91 IEE226 pKa = 4.71 KK227 pKa = 9.52 PAYY230 pKa = 9.0 KK231 pKa = 10.22 LLSADD236 pKa = 3.23 RR237 pKa = 11.84 WDD239 pKa = 3.9 VFTPPTKK246 pKa = 10.75 AGDD249 pKa = 3.8 SRR251 pKa = 11.84 DD252 pKa = 3.77 GNTGLSPLGGG262 pKa = 3.52
Molecular weight: 29.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.766
IPC2_protein 6.927
IPC_protein 7.044
Toseland 6.942
ProMoST 7.497
Dawson 7.644
Bjellqvist 7.658
Wikipedia 7.585
Rodwell 7.629
Grimsley 7.044
Solomon 7.732
Lehninger 7.746
Nozaki 7.892
DTASelect 7.805
Thurlkill 7.834
EMBOSS 7.922
Sillero 8.053
Patrickios 4.113
IPC_peptide 7.732
IPC2_peptide 7.234
IPC2.peptide.svr19 7.248
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
606
262
344
303.0
34.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.125 ± 0.044
1.155 ± 0.453
6.931 ± 1.342
5.941 ± 0.589
2.805 ± 0.999
9.076 ± 0.97
2.145 ± 0.776
5.776 ± 0.659
5.116 ± 1.056
7.261 ± 0.465
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.135 ± 0.738
4.95 ± 1.372
4.455 ± 0.844
3.3 ± 0.378
6.436 ± 0.721
6.436 ± 0.887
6.766 ± 0.063
7.426 ± 0.794
1.485 ± 0.255
5.281 ± 0.727
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here