Amino acid dipepetide frequency for Lactococcus phage TP712

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.898AlaAla: 3.898 ± 0.971
0.239AlaCys: 0.239 ± 0.137
3.262AlaAsp: 3.262 ± 0.45
4.296AlaGlu: 4.296 ± 0.686
3.103AlaPhe: 3.103 ± 0.618
3.182AlaGly: 3.182 ± 0.588
1.193AlaHis: 1.193 ± 0.276
6.762AlaIle: 6.762 ± 0.823
5.967AlaLys: 5.967 ± 1.014
5.489AlaLeu: 5.489 ± 0.734
2.148AlaMet: 2.148 ± 0.5
3.739AlaAsn: 3.739 ± 0.548
0.796AlaPro: 0.796 ± 0.319
1.909AlaGln: 1.909 ± 0.377
2.864AlaArg: 2.864 ± 0.511
4.535AlaSer: 4.535 ± 0.662
5.33AlaThr: 5.33 ± 0.882
4.057AlaVal: 4.057 ± 0.689
1.193AlaTrp: 1.193 ± 0.616
2.944AlaTyr: 2.944 ± 0.433
0.0AlaXaa: 0.0 ± 0.0
Cys
0.318CysAla: 0.318 ± 0.172
0.08CysCys: 0.08 ± 0.084
0.398CysAsp: 0.398 ± 0.186
0.398CysGlu: 0.398 ± 0.17
0.159CysPhe: 0.159 ± 0.119
0.318CysGly: 0.318 ± 0.182
0.159CysHis: 0.159 ± 0.121
0.239CysIle: 0.239 ± 0.163
0.636CysLys: 0.636 ± 0.246
0.398CysLeu: 0.398 ± 0.203
0.08CysMet: 0.08 ± 0.081
0.08CysAsn: 0.08 ± 0.095
0.0CysPro: 0.0 ± 0.0
0.0CysGln: 0.0 ± 0.0
0.318CysArg: 0.318 ± 0.201
0.318CysSer: 0.318 ± 0.134
0.398CysThr: 0.398 ± 0.187
0.239CysVal: 0.239 ± 0.136
0.08CysTrp: 0.08 ± 0.074
0.239CysTyr: 0.239 ± 0.171
0.0CysXaa: 0.0 ± 0.0
Asp
3.421AspAla: 3.421 ± 0.675
0.477AspCys: 0.477 ± 0.181
3.739AspAsp: 3.739 ± 0.479
5.171AspGlu: 5.171 ± 0.79
2.784AspPhe: 2.784 ± 0.427
4.455AspGly: 4.455 ± 0.551
0.716AspHis: 0.716 ± 0.201
4.773AspIle: 4.773 ± 0.764
5.648AspLys: 5.648 ± 0.642
4.694AspLeu: 4.694 ± 0.837
1.034AspMet: 1.034 ± 0.276
4.535AspAsn: 4.535 ± 0.492
1.909AspPro: 1.909 ± 0.366
1.83AspGln: 1.83 ± 0.408
2.307AspArg: 2.307 ± 0.482
3.5AspSer: 3.5 ± 0.68
2.546AspThr: 2.546 ± 0.471
3.58AspVal: 3.58 ± 0.48
1.034AspTrp: 1.034 ± 0.224
3.58AspTyr: 3.58 ± 0.471
0.0AspXaa: 0.0 ± 0.0
Glu
4.932GluAla: 4.932 ± 0.809
0.239GluCys: 0.239 ± 0.126
2.944GluAsp: 2.944 ± 0.517
6.205GluGlu: 6.205 ± 0.948
3.421GluPhe: 3.421 ± 0.502
3.182GluGly: 3.182 ± 0.56
1.591GluHis: 1.591 ± 0.485
6.046GluIle: 6.046 ± 0.702
7.16GluLys: 7.16 ± 1.045
7.399GluLeu: 7.399 ± 1.075
1.671GluMet: 1.671 ± 0.345
4.216GluAsn: 4.216 ± 0.604
0.955GluPro: 0.955 ± 0.307
3.58GluGln: 3.58 ± 0.771
2.944GluArg: 2.944 ± 0.419
4.375GluSer: 4.375 ± 0.569
3.739GluThr: 3.739 ± 0.642
4.057GluVal: 4.057 ± 0.555
0.875GluTrp: 0.875 ± 0.257
2.387GluTyr: 2.387 ± 0.488
0.0GluXaa: 0.0 ± 0.0
Phe
2.784PheAla: 2.784 ± 0.467
0.239PheCys: 0.239 ± 0.129
2.944PheAsp: 2.944 ± 0.472
2.944PheGlu: 2.944 ± 0.487
1.432PhePhe: 1.432 ± 0.4
3.58PheGly: 3.58 ± 0.417
0.716PheHis: 0.716 ± 0.327
2.625PheIle: 2.625 ± 0.479
4.455PheLys: 4.455 ± 0.468
4.057PheLeu: 4.057 ± 0.783
1.273PheMet: 1.273 ± 0.374
2.705PheAsn: 2.705 ± 0.46
1.114PhePro: 1.114 ± 0.247
0.875PheGln: 0.875 ± 0.299
1.273PheArg: 1.273 ± 0.323
3.262PheSer: 3.262 ± 0.536
2.864PheThr: 2.864 ± 0.463
1.83PheVal: 1.83 ± 0.36
0.239PheTrp: 0.239 ± 0.125
1.512PheTyr: 1.512 ± 0.414
0.0PheXaa: 0.0 ± 0.0
Gly
4.694GlyAla: 4.694 ± 0.974
0.08GlyCys: 0.08 ± 0.079
4.614GlyAsp: 4.614 ± 0.698
3.5GlyGlu: 3.5 ± 0.393
2.705GlyPhe: 2.705 ± 0.431
4.773GlyGly: 4.773 ± 1.158
0.955GlyHis: 0.955 ± 0.323
4.535GlyIle: 4.535 ± 0.631
5.41GlyLys: 5.41 ± 0.502
5.41GlyLeu: 5.41 ± 0.731
1.83GlyMet: 1.83 ± 0.334
4.137GlyAsn: 4.137 ± 0.581
0.875GlyPro: 0.875 ± 0.265
2.864GlyGln: 2.864 ± 0.529
2.228GlyArg: 2.228 ± 0.365
4.375GlySer: 4.375 ± 0.721
4.296GlyThr: 4.296 ± 0.827
3.66GlyVal: 3.66 ± 0.663
0.398GlyTrp: 0.398 ± 0.264
2.944GlyTyr: 2.944 ± 0.446
0.0GlyXaa: 0.0 ± 0.0
His
0.875HisAla: 0.875 ± 0.227
0.318HisCys: 0.318 ± 0.164
0.557HisAsp: 0.557 ± 0.202
1.432HisGlu: 1.432 ± 0.459
0.636HisPhe: 0.636 ± 0.226
0.796HisGly: 0.796 ± 0.329
0.239HisHis: 0.239 ± 0.151
1.034HisIle: 1.034 ± 0.308
1.114HisLys: 1.114 ± 0.36
0.955HisLeu: 0.955 ± 0.34
0.318HisMet: 0.318 ± 0.17
0.477HisAsn: 0.477 ± 0.215
0.796HisPro: 0.796 ± 0.285
0.716HisGln: 0.716 ± 0.215
0.159HisArg: 0.159 ± 0.112
0.875HisSer: 0.875 ± 0.275
0.398HisThr: 0.398 ± 0.159
0.716HisVal: 0.716 ± 0.238
0.398HisTrp: 0.398 ± 0.185
0.955HisTyr: 0.955 ± 0.288
0.0HisXaa: 0.0 ± 0.0
Ile
5.171IleAla: 5.171 ± 0.848
0.318IleCys: 0.318 ± 0.164
5.251IleAsp: 5.251 ± 0.6
4.853IleGlu: 4.853 ± 0.772
1.75IlePhe: 1.75 ± 0.419
6.285IleGly: 6.285 ± 1.309
1.114IleHis: 1.114 ± 0.283
4.137IleIle: 4.137 ± 0.684
6.762IleLys: 6.762 ± 0.642
5.648IleLeu: 5.648 ± 0.601
1.432IleMet: 1.432 ± 0.262
5.489IleAsn: 5.489 ± 0.785
2.068IlePro: 2.068 ± 0.415
3.5IleGln: 3.5 ± 0.568
2.387IleArg: 2.387 ± 0.512
6.126IleSer: 6.126 ± 0.548
4.296IleThr: 4.296 ± 0.623
3.66IleVal: 3.66 ± 0.62
0.636IleTrp: 0.636 ± 0.236
3.5IleTyr: 3.5 ± 0.625
0.0IleXaa: 0.0 ± 0.0
Lys
7.399LysAla: 7.399 ± 0.689
0.239LysCys: 0.239 ± 0.133
3.898LysAsp: 3.898 ± 0.544
7.399LysGlu: 7.399 ± 0.894
3.739LysPhe: 3.739 ± 0.464
4.535LysGly: 4.535 ± 0.55
0.875LysHis: 0.875 ± 0.317
5.489LysIle: 5.489 ± 0.982
7.558LysLys: 7.558 ± 0.939
6.603LysLeu: 6.603 ± 0.868
2.466LysMet: 2.466 ± 0.412
5.171LysAsn: 5.171 ± 0.749
2.546LysPro: 2.546 ± 0.344
3.739LysGln: 3.739 ± 0.713
3.978LysArg: 3.978 ± 0.829
4.932LysSer: 4.932 ± 0.707
6.364LysThr: 6.364 ± 0.708
6.523LysVal: 6.523 ± 0.653
1.83LysTrp: 1.83 ± 0.521
4.535LysTyr: 4.535 ± 0.667
0.0LysXaa: 0.0 ± 0.0
Leu
4.853LeuAla: 4.853 ± 0.523
0.239LeuCys: 0.239 ± 0.166
5.091LeuAsp: 5.091 ± 0.869
6.364LeuGlu: 6.364 ± 0.635
4.137LeuPhe: 4.137 ± 0.8
5.33LeuGly: 5.33 ± 0.772
0.796LeuHis: 0.796 ± 0.259
5.33LeuIle: 5.33 ± 0.719
7.876LeuLys: 7.876 ± 0.861
6.205LeuLeu: 6.205 ± 0.892
1.591LeuMet: 1.591 ± 0.32
5.489LeuAsn: 5.489 ± 0.474
3.262LeuPro: 3.262 ± 0.525
3.898LeuGln: 3.898 ± 0.535
2.466LeuArg: 2.466 ± 0.442
6.842LeuSer: 6.842 ± 0.745
5.489LeuThr: 5.489 ± 0.749
4.057LeuVal: 4.057 ± 0.768
0.477LeuTrp: 0.477 ± 0.186
2.068LeuTyr: 2.068 ± 0.448
0.0LeuXaa: 0.0 ± 0.0
Met
1.909MetAla: 1.909 ± 0.41
0.08MetCys: 0.08 ± 0.095
1.193MetAsp: 1.193 ± 0.313
1.114MetGlu: 1.114 ± 0.269
1.114MetPhe: 1.114 ± 0.305
1.273MetGly: 1.273 ± 0.563
0.239MetHis: 0.239 ± 0.145
1.273MetIle: 1.273 ± 0.281
2.705MetLys: 2.705 ± 0.432
1.114MetLeu: 1.114 ± 0.285
0.318MetMet: 0.318 ± 0.224
2.068MetAsn: 2.068 ± 0.385
0.796MetPro: 0.796 ± 0.258
0.796MetGln: 0.796 ± 0.255
0.796MetArg: 0.796 ± 0.239
2.228MetSer: 2.228 ± 0.316
1.83MetThr: 1.83 ± 0.367
1.193MetVal: 1.193 ± 0.234
0.239MetTrp: 0.239 ± 0.135
0.716MetTyr: 0.716 ± 0.229
0.0MetXaa: 0.0 ± 0.0
Asn
3.739AsnAla: 3.739 ± 0.799
0.398AsnCys: 0.398 ± 0.217
4.694AsnAsp: 4.694 ± 0.62
4.216AsnGlu: 4.216 ± 0.651
1.989AsnPhe: 1.989 ± 0.407
4.773AsnGly: 4.773 ± 0.751
0.796AsnHis: 0.796 ± 0.299
5.33AsnIle: 5.33 ± 0.724
4.455AsnLys: 4.455 ± 0.618
5.171AsnLeu: 5.171 ± 0.64
1.114AsnMet: 1.114 ± 0.226
3.5AsnAsn: 3.5 ± 0.656
2.148AsnPro: 2.148 ± 0.408
3.103AsnGln: 3.103 ± 0.489
1.75AsnArg: 1.75 ± 0.375
4.535AsnSer: 4.535 ± 0.707
2.705AsnThr: 2.705 ± 0.42
3.262AsnVal: 3.262 ± 0.436
0.955AsnTrp: 0.955 ± 0.239
2.705AsnTyr: 2.705 ± 0.469
0.0AsnXaa: 0.0 ± 0.0
Pro
1.75ProAla: 1.75 ± 0.486
0.0ProCys: 0.0 ± 0.0
2.148ProAsp: 2.148 ± 0.373
1.989ProGlu: 1.989 ± 0.347
1.432ProPhe: 1.432 ± 0.34
0.955ProGly: 0.955 ± 0.318
0.557ProHis: 0.557 ± 0.207
1.83ProIle: 1.83 ± 0.379
2.944ProLys: 2.944 ± 0.467
2.228ProLeu: 2.228 ± 0.375
0.398ProMet: 0.398 ± 0.221
2.068ProAsn: 2.068 ± 0.483
0.477ProPro: 0.477 ± 0.184
1.193ProGln: 1.193 ± 0.283
0.557ProArg: 0.557 ± 0.171
2.228ProSer: 2.228 ± 0.456
1.273ProThr: 1.273 ± 0.308
1.591ProVal: 1.591 ± 0.317
0.318ProTrp: 0.318 ± 0.165
1.114ProTyr: 1.114 ± 0.211
0.0ProXaa: 0.0 ± 0.0
Gln
4.296GlnAla: 4.296 ± 0.546
0.0GlnCys: 0.0 ± 0.0
1.909GlnAsp: 1.909 ± 0.35
3.421GlnGlu: 3.421 ± 0.564
1.909GlnPhe: 1.909 ± 0.343
2.068GlnGly: 2.068 ± 0.373
0.477GlnHis: 0.477 ± 0.194
2.625GlnIle: 2.625 ± 0.458
3.341GlnLys: 3.341 ± 0.447
4.375GlnLeu: 4.375 ± 0.641
0.796GlnMet: 0.796 ± 0.29
1.432GlnAsn: 1.432 ± 0.292
1.273GlnPro: 1.273 ± 0.29
1.671GlnGln: 1.671 ± 0.455
1.83GlnArg: 1.83 ± 0.34
3.262GlnSer: 3.262 ± 0.584
2.784GlnThr: 2.784 ± 0.423
2.387GlnVal: 2.387 ± 0.344
0.318GlnTrp: 0.318 ± 0.126
0.875GlnTyr: 0.875 ± 0.229
0.0GlnXaa: 0.0 ± 0.0
Arg
1.512ArgAla: 1.512 ± 0.372
0.239ArgCys: 0.239 ± 0.162
2.148ArgAsp: 2.148 ± 0.457
2.705ArgGlu: 2.705 ± 0.541
1.83ArgPhe: 1.83 ± 0.488
1.432ArgGly: 1.432 ± 0.293
0.318ArgHis: 0.318 ± 0.152
3.5ArgIle: 3.5 ± 0.551
2.228ArgLys: 2.228 ± 0.47
4.216ArgLeu: 4.216 ± 0.838
1.273ArgMet: 1.273 ± 0.252
1.83ArgAsn: 1.83 ± 0.352
0.955ArgPro: 0.955 ± 0.296
1.671ArgGln: 1.671 ± 0.312
1.83ArgArg: 1.83 ± 0.405
1.83ArgSer: 1.83 ± 0.39
1.909ArgThr: 1.909 ± 0.434
1.909ArgVal: 1.909 ± 0.369
0.398ArgTrp: 0.398 ± 0.154
1.909ArgTyr: 1.909 ± 0.411
0.0ArgXaa: 0.0 ± 0.0
Ser
4.773SerAla: 4.773 ± 0.916
0.477SerCys: 0.477 ± 0.159
4.773SerAsp: 4.773 ± 0.498
4.694SerGlu: 4.694 ± 0.502
2.625SerPhe: 2.625 ± 0.462
5.489SerGly: 5.489 ± 0.758
1.034SerHis: 1.034 ± 0.262
5.728SerIle: 5.728 ± 0.784
7.399SerLys: 7.399 ± 0.898
4.773SerLeu: 4.773 ± 0.611
2.148SerMet: 2.148 ± 0.489
4.694SerAsn: 4.694 ± 0.656
1.193SerPro: 1.193 ± 0.306
2.387SerGln: 2.387 ± 0.452
2.466SerArg: 2.466 ± 0.458
4.375SerSer: 4.375 ± 0.685
4.296SerThr: 4.296 ± 0.618
4.375SerVal: 4.375 ± 0.611
0.716SerTrp: 0.716 ± 0.245
2.307SerTyr: 2.307 ± 0.54
0.0SerXaa: 0.0 ± 0.0
Thr
4.296ThrAla: 4.296 ± 0.745
0.159ThrCys: 0.159 ± 0.109
4.057ThrAsp: 4.057 ± 0.477
4.455ThrGlu: 4.455 ± 0.584
2.864ThrPhe: 2.864 ± 0.571
5.33ThrGly: 5.33 ± 0.864
0.875ThrHis: 0.875 ± 0.276
5.569ThrIle: 5.569 ± 0.553
5.012ThrLys: 5.012 ± 0.641
4.614ThrLeu: 4.614 ± 0.648
0.557ThrMet: 0.557 ± 0.197
2.625ThrAsn: 2.625 ± 0.49
2.387ThrPro: 2.387 ± 0.524
1.83ThrGln: 1.83 ± 0.38
1.512ThrArg: 1.512 ± 0.326
3.421ThrSer: 3.421 ± 0.509
3.023ThrThr: 3.023 ± 0.533
4.694ThrVal: 4.694 ± 0.851
0.477ThrTrp: 0.477 ± 0.197
2.068ThrTyr: 2.068 ± 0.512
0.0ThrXaa: 0.0 ± 0.0
Val
3.262ValAla: 3.262 ± 0.697
0.239ValCys: 0.239 ± 0.164
4.535ValAsp: 4.535 ± 0.604
3.898ValGlu: 3.898 ± 0.707
2.705ValPhe: 2.705 ± 0.516
3.5ValGly: 3.5 ± 0.613
0.716ValHis: 0.716 ± 0.238
4.375ValIle: 4.375 ± 0.55
5.33ValLys: 5.33 ± 0.67
4.216ValLeu: 4.216 ± 0.67
1.193ValMet: 1.193 ± 0.296
3.898ValAsn: 3.898 ± 0.544
1.75ValPro: 1.75 ± 0.355
2.148ValGln: 2.148 ± 0.322
1.83ValArg: 1.83 ± 0.38
5.251ValSer: 5.251 ± 0.621
3.421ValThr: 3.421 ± 0.88
3.182ValVal: 3.182 ± 0.558
0.477ValTrp: 0.477 ± 0.155
1.671ValTyr: 1.671 ± 0.35
0.0ValXaa: 0.0 ± 0.0
Trp
0.477TrpAla: 0.477 ± 0.22
0.08TrpCys: 0.08 ± 0.083
1.273TrpAsp: 1.273 ± 0.502
0.716TrpGlu: 0.716 ± 0.199
0.955TrpPhe: 0.955 ± 0.21
0.477TrpGly: 0.477 ± 0.217
0.0TrpHis: 0.0 ± 0.0
0.875TrpIle: 0.875 ± 0.237
0.398TrpLys: 0.398 ± 0.158
1.273TrpLeu: 1.273 ± 0.314
0.477TrpMet: 0.477 ± 0.179
1.034TrpAsn: 1.034 ± 0.378
0.0TrpPro: 0.0 ± 0.0
0.636TrpGln: 0.636 ± 0.212
0.239TrpArg: 0.239 ± 0.125
0.955TrpSer: 0.955 ± 0.266
0.636TrpThr: 0.636 ± 0.267
0.796TrpVal: 0.796 ± 0.245
0.0TrpTrp: 0.0 ± 0.0
0.398TrpTyr: 0.398 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.546TyrAla: 2.546 ± 0.477
0.716TyrCys: 0.716 ± 0.25
2.307TyrAsp: 2.307 ± 0.415
2.148TyrGlu: 2.148 ± 0.447
1.432TyrPhe: 1.432 ± 0.376
2.546TyrGly: 2.546 ± 0.471
0.398TyrHis: 0.398 ± 0.189
2.307TyrIle: 2.307 ± 0.546
3.421TyrLys: 3.421 ± 0.635
3.023TyrLeu: 3.023 ± 0.485
0.796TyrMet: 0.796 ± 0.2
2.148TyrAsn: 2.148 ± 0.4
1.75TyrPro: 1.75 ± 0.407
2.705TyrGln: 2.705 ± 0.35
1.909TyrArg: 1.909 ± 0.416
3.5TyrSer: 3.5 ± 0.562
2.228TyrThr: 2.228 ± 0.36
1.83TyrVal: 1.83 ± 0.286
0.636TyrTrp: 0.636 ± 0.228
1.512TyrTyr: 1.512 ± 0.428
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (12571 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski