Gordonia phage EpicDab
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6E509|A0A4D6E509_9CAUD Uncharacterized protein OS=Gordonia phage EpicDab OX=2608086 GN=21 PE=4 SV=1
MM1 pKa = 7.74 AIDD4 pKa = 3.57 IAAGVRR10 pKa = 11.84 AVIAEE15 pKa = 4.3 LEE17 pKa = 4.37 TAGIAAYY24 pKa = 9.94 DD25 pKa = 3.81 SPAKK29 pKa = 10.49 VNPPCAYY36 pKa = 9.87 VQPTKK41 pKa = 10.97 LDD43 pKa = 3.31 GFTLGDD49 pKa = 3.83 CAEE52 pKa = 4.3 LTVQVFLCAPANSAEE67 pKa = 4.34 VVEE70 pKa = 4.71 PMLGTLLNAALGVLDD85 pKa = 3.58 PTEE88 pKa = 4.44 PVTLNATLALDD99 pKa = 4.02 VGQVPCYY106 pKa = 10.03 IVTASTDD113 pKa = 3.14 TT114 pKa = 3.64
Molecular weight: 11.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.88
IPC2_protein 3.681
IPC_protein 3.579
Toseland 3.401
ProMoST 3.783
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.554
Rodwell 3.427
Grimsley 3.325
Solomon 3.554
Lehninger 3.503
Nozaki 3.732
DTASelect 3.897
Thurlkill 3.478
EMBOSS 3.554
Sillero 3.719
Patrickios 0.121
IPC_peptide 3.541
IPC2_peptide 3.681
IPC2.peptide.svr19 3.691
Protein with the highest isoelectric point:
>tr|A0A4D6E3W7|A0A4D6E3W7_9CAUD Uncharacterized protein OS=Gordonia phage EpicDab OX=2608086 GN=23 PE=4 SV=1
MM1 pKa = 7.99 ADD3 pKa = 2.91 AQLRR7 pKa = 11.84 VDD9 pKa = 4.22 GARR12 pKa = 11.84 QLRR15 pKa = 11.84 TSMRR19 pKa = 11.84 RR20 pKa = 11.84 AGIDD24 pKa = 3.17 MRR26 pKa = 11.84 EE27 pKa = 4.1 LRR29 pKa = 11.84 DD30 pKa = 3.48 THH32 pKa = 8.24 AEE34 pKa = 3.88 AARR37 pKa = 11.84 LVAARR42 pKa = 11.84 ADD44 pKa = 3.59 PPRR47 pKa = 11.84 RR48 pKa = 11.84 TGRR51 pKa = 11.84 LAATIRR57 pKa = 11.84 ASGTQTQAVVRR68 pKa = 11.84 AGYY71 pKa = 10.73 ARR73 pKa = 11.84 VPYY76 pKa = 10.38 APVIHH81 pKa = 6.77 WGWPARR87 pKa = 11.84 GIRR90 pKa = 11.84 AQPFLTDD97 pKa = 3.6 AAQASEE103 pKa = 4.17 PAWIEE108 pKa = 3.71 LYY110 pKa = 9.63 TRR112 pKa = 11.84 HH113 pKa = 5.77 VEE115 pKa = 4.34 QILTTIEE122 pKa = 4.23 GASTPP127 pKa = 3.79
Molecular weight: 13.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.198
IPC2_protein 9.794
IPC_protein 11.14
Toseland 11.125
ProMoST 11.608
Dawson 11.155
Bjellqvist 11.14
Wikipedia 11.623
Rodwell 10.716
Grimsley 11.213
Solomon 11.623
Lehninger 11.52
Nozaki 11.125
DTASelect 11.14
Thurlkill 11.125
EMBOSS 11.623
Sillero 11.14
Patrickios 10.467
IPC_peptide 11.623
IPC2_peptide 10.613
IPC2.peptide.svr19 9.437
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24
0
24
5225
60
628
217.7
23.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.392 ± 0.849
0.842 ± 0.204
6.488 ± 0.389
4.823 ± 0.259
2.718 ± 0.269
9.206 ± 0.878
2.373 ± 0.328
4.191 ± 0.339
2.258 ± 0.339
7.579 ± 0.388
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.761 ± 0.203
2.469 ± 0.27
7.196 ± 0.57
3.081 ± 0.242
7.522 ± 0.74
4.498 ± 0.297
7.196 ± 0.597
7.215 ± 0.537
2.278 ± 0.276
1.914 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here