Ralstonia phage RSM3

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Habenivirus; Ralstonia virus RSM3

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5UAR9|B5UAR9_9VIRU Uncharacterized protein OS=Ralstonia phage RSM3 OX=520962 PE=4 SV=1
MM1 pKa = 7.16AQCLVIQNGGLVFTTDD17 pKa = 3.98PPQSCSGYY25 pKa = 11.04LLLQPSEE32 pKa = 4.3YY33 pKa = 10.14SNVMALSGAFTYY45 pKa = 10.29PSASDD50 pKa = 3.36FAAAFTAGFQWPVFFFIVAMLVSKK74 pKa = 10.36VASFFDD80 pKa = 3.56RR81 pKa = 11.84GG82 pKa = 3.31

Molecular weight: 8.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5UAR7|B5UAR7_9VIRU Uncharacterized protein OS=Ralstonia phage RSM3 OX=520962 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.39LPNPLWLLLVGWLATAAAHH21 pKa = 5.53NAWRR25 pKa = 11.84LFSVIGFAGVSMLIQGGHH43 pKa = 4.23VVARR47 pKa = 11.84ACLLLAVVVVVVRR60 pKa = 11.84LVRR63 pKa = 11.84RR64 pKa = 11.84FFRR67 pKa = 11.84RR68 pKa = 11.84AA69 pKa = 2.88

Molecular weight: 7.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

2491

65

511

166.1

17.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.408 ± 0.643

1.325 ± 0.287

4.496 ± 0.276

4.175 ± 1.083

3.292 ± 0.704

7.949 ± 1.157

1.766 ± 0.436

4.135 ± 0.361

4.135 ± 0.668

7.908 ± 1.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.569 ± 0.352

3.212 ± 0.4

4.336 ± 0.582

4.737 ± 0.626

5.982 ± 1.13

6.383 ± 0.768

6.544 ± 1.365

9.153 ± 0.763

2.248 ± 0.385

2.248 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski