Komagataeibacter medellinensis (strain NBRC 3288 / BCRC 11682 / LMG 1693 / Kondo 51) (Gluconacetobacter medellinensis)
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3116 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G2I5K6|G2I5K6_KOMMN Ribosomal RNA small subunit methyltransferase A OS=Komagataeibacter medellinensis (strain NBRC 3288 / BCRC 11682 / LMG 1693 / Kondo 51) OX=634177 GN=rsmA PE=3 SV=1
MM1 pKa = 7.7 AATGLQQVEE10 pKa = 4.96 GIEE13 pKa = 4.01 NMEE16 pKa = 4.28 TLTINIGGMTCDD28 pKa = 3.34 GCASKK33 pKa = 10.75 VVHH36 pKa = 6.31 ALEE39 pKa = 4.34 SVEE42 pKa = 4.11 GVSMVQVSLEE52 pKa = 4.05 KK53 pKa = 10.95 GKK55 pKa = 9.19 ATVTYY60 pKa = 10.11 DD61 pKa = 3.21 GRR63 pKa = 11.84 YY64 pKa = 8.13 TNVDD68 pKa = 3.62 DD69 pKa = 5.98 LFAAVDD75 pKa = 3.83 DD76 pKa = 4.8 AGFDD80 pKa = 3.06 AGYY83 pKa = 11.01
Molecular weight: 8.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.792
IPC2_protein 4.012
IPC_protein 3.91
Toseland 3.719
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.834
Nozaki 4.024
DTASelect 4.202
Thurlkill 3.783
EMBOSS 3.834
Sillero 4.024
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.923
Protein with the highest isoelectric point:
>tr|G2I861|G2I861_KOMMN Uncharacterized protein OS=Komagataeibacter medellinensis (strain NBRC 3288 / BCRC 11682 / LMG 1693 / Kondo 51) OX=634177 GN=GLX_29500 PE=4 SV=1
MM1 pKa = 7.39 TPGLQRR7 pKa = 11.84 EE8 pKa = 4.33 AVTWAMTEE16 pKa = 3.4 RR17 pKa = 11.84 DD18 pKa = 3.54 YY19 pKa = 11.57 SQRR22 pKa = 11.84 RR23 pKa = 11.84 ACRR26 pKa = 11.84 LIGMDD31 pKa = 3.7 PKK33 pKa = 10.22 TWRR36 pKa = 11.84 CASRR40 pKa = 11.84 RR41 pKa = 11.84 PDD43 pKa = 3.44 DD44 pKa = 3.84 AEE46 pKa = 3.85 ARR48 pKa = 11.84 SRR50 pKa = 11.84 LRR52 pKa = 11.84 DD53 pKa = 3.33 LAGEE57 pKa = 4.32 RR58 pKa = 11.84 GRR60 pKa = 11.84 FGYY63 pKa = 10.13 RR64 pKa = 11.84 RR65 pKa = 11.84 LHH67 pKa = 6.04 ILLKK71 pKa = 10.64 RR72 pKa = 11.84 EE73 pKa = 4.01 GVTMNHH79 pKa = 6.21 KK80 pKa = 10.36 KK81 pKa = 10.48 LFRR84 pKa = 11.84 LYY86 pKa = 10.26 RR87 pKa = 11.84 EE88 pKa = 4.15 EE89 pKa = 4.08 GLSVRR94 pKa = 11.84 KK95 pKa = 9.99 RR96 pKa = 11.84 GGRR99 pKa = 11.84 KK100 pKa = 8.5 RR101 pKa = 11.84 AMGTRR106 pKa = 11.84 SPMMLPEE113 pKa = 4.93 GPNQRR118 pKa = 11.84 WSLDD122 pKa = 3.71 FVSDD126 pKa = 3.42 ALNNGRR132 pKa = 11.84 RR133 pKa = 11.84 FRR135 pKa = 11.84 VLTVVDD141 pKa = 4.79 DD142 pKa = 3.91 YY143 pKa = 11.77 TRR145 pKa = 11.84 EE146 pKa = 4.04 CLALVADD153 pKa = 4.69 TSLSGEE159 pKa = 3.97 RR160 pKa = 11.84 LGRR163 pKa = 11.84 EE164 pKa = 3.68 LDD166 pKa = 3.77 RR167 pKa = 11.84 IGEE170 pKa = 4.15 HH171 pKa = 6.46 RR172 pKa = 11.84 GFPMMIVSDD181 pKa = 3.75 DD182 pKa = 3.46 VLYY185 pKa = 10.98 RR186 pKa = 11.84 EE187 pKa = 4.01 ASGRR191 pKa = 11.84 FGNTSPSAVCTVVEE205 pKa = 5.29 KK206 pKa = 11.09 YY207 pKa = 10.4 CKK209 pKa = 9.56 QKK211 pKa = 10.73 PGGLHH216 pKa = 6.42 RR217 pKa = 11.84 KK218 pKa = 9.17 IKK220 pKa = 9.97 VLPAIANTEE229 pKa = 4.05 SRR231 pKa = 11.84 SAA233 pKa = 3.3
Molecular weight: 26.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.516
IPC_protein 10.35
Toseland 10.57
ProMoST 10.306
Dawson 10.687
Bjellqvist 10.409
Wikipedia 10.906
Rodwell 10.804
Grimsley 10.73
Solomon 10.804
Lehninger 10.774
Nozaki 10.584
DTASelect 10.409
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.613
Patrickios 10.496
IPC_peptide 10.804
IPC2_peptide 9.545
IPC2.peptide.svr19 8.631
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3116
0
3116
994498
26
2848
319.2
34.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.013 ± 0.062
1.11 ± 0.016
5.531 ± 0.029
4.845 ± 0.043
3.355 ± 0.028
8.577 ± 0.037
2.658 ± 0.02
4.94 ± 0.032
2.491 ± 0.037
10.026 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.958 ± 0.022
2.638 ± 0.028
5.71 ± 0.036
3.541 ± 0.028
7.568 ± 0.047
5.284 ± 0.028
5.785 ± 0.03
7.312 ± 0.042
1.437 ± 0.019
2.22 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here