Synechococcus phage S-CBP3
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A096VKK6|A0A096VKK6_9CAUD Primase/helicase OS=Synechococcus phage S-CBP3 OX=756276 GN=S-CBP3_0033 PE=4 SV=1
MM1 pKa = 7.38 MYY3 pKa = 10.94 AVIQDD8 pKa = 3.85 DD9 pKa = 3.99 AVIEE13 pKa = 4.14 YY14 pKa = 10.61 FGDD17 pKa = 3.09 EE18 pKa = 3.57 TAAYY22 pKa = 10.4 NEE24 pKa = 4.3 AFNQLEE30 pKa = 4.39 HH31 pKa = 6.74 EE32 pKa = 4.47 VDD34 pKa = 3.59 GPVYY38 pKa = 10.23 VVEE41 pKa = 3.83 IVKK44 pKa = 10.6 RR45 pKa = 11.84 FDD47 pKa = 2.98
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.786
IPC2_protein 3.872
IPC_protein 3.732
Toseland 3.554
ProMoST 3.795
Dawson 3.719
Bjellqvist 3.973
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.478
Solomon 3.681
Lehninger 3.63
Nozaki 3.859
DTASelect 3.999
Thurlkill 3.63
EMBOSS 3.668
Sillero 3.846
Patrickios 0.604
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A096VKJ8|A0A096VKJ8_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBP3 OX=756276 GN=S-CBP3_0023 PE=4 SV=1
MM1 pKa = 7.4 KK2 pKa = 10.47 LSRR5 pKa = 11.84 FGGKK9 pKa = 7.87 VWWLKK14 pKa = 10.67 IEE16 pKa = 4.19 TLLTLNRR23 pKa = 11.84 EE24 pKa = 4.25 SRR26 pKa = 11.84 DD27 pKa = 2.9 QDD29 pKa = 3.49 LQTRR33 pKa = 11.84 RR34 pKa = 11.84 LVPAYY39 pKa = 10.2 RR40 pKa = 11.84 GGQVLVGIRR49 pKa = 11.84 LYY51 pKa = 10.61 RR52 pKa = 11.84 IRR54 pKa = 11.84 WDD56 pKa = 3.42 GLRR59 pKa = 11.84 CNGYY63 pKa = 8.93 QSCLSVASDD72 pKa = 4.07 LLISS76 pKa = 3.88
Molecular weight: 8.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.604
IPC_protein 10.423
Toseland 10.335
ProMoST 10.116
Dawson 10.511
Bjellqvist 10.277
Wikipedia 10.745
Rodwell 10.657
Grimsley 10.599
Solomon 10.613
Lehninger 10.57
Nozaki 10.365
DTASelect 10.262
Thurlkill 10.379
EMBOSS 10.745
Sillero 10.452
Patrickios 10.423
IPC_peptide 10.599
IPC2_peptide 9.487
IPC2.peptide.svr19 8.429
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
53
0
53
12571
44
991
237.2
26.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.522 ± 0.51
0.7 ± 0.151
6.229 ± 0.251
6.499 ± 0.431
3.532 ± 0.238
7.167 ± 0.407
1.671 ± 0.196
4.988 ± 0.303
5.513 ± 0.484
8.504 ± 0.427
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.49 ± 0.214
5.266 ± 0.314
4.017 ± 0.203
4.646 ± 0.331
5.258 ± 0.291
6.634 ± 0.381
6.213 ± 0.533
6.054 ± 0.275
1.281 ± 0.141
3.818 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here