Amino acid dipepetide frequency for Saccharospirillum sp. MSK14-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.561AlaAla: 10.561 ± 0.122
0.851AlaCys: 0.851 ± 0.032
6.792AlaAsp: 6.792 ± 0.072
6.787AlaGlu: 6.787 ± 0.085
3.807AlaPhe: 3.807 ± 0.059
8.036AlaGly: 8.036 ± 0.094
1.937AlaHis: 1.937 ± 0.049
5.355AlaIle: 5.355 ± 0.079
2.909AlaLys: 2.909 ± 0.065
12.712AlaLeu: 12.712 ± 0.147
2.794AlaMet: 2.794 ± 0.048
3.175AlaAsn: 3.175 ± 0.057
3.67AlaPro: 3.67 ± 0.064
4.817AlaGln: 4.817 ± 0.075
5.935AlaArg: 5.935 ± 0.086
5.856AlaSer: 5.856 ± 0.072
4.871AlaThr: 4.871 ± 0.072
7.555AlaVal: 7.555 ± 0.092
1.463AlaTrp: 1.463 ± 0.037
2.39AlaTyr: 2.39 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.843CysAla: 0.843 ± 0.025
0.122CysCys: 0.122 ± 0.011
0.525CysAsp: 0.525 ± 0.021
0.454CysGlu: 0.454 ± 0.021
0.359CysPhe: 0.359 ± 0.019
0.769CysGly: 0.769 ± 0.029
0.262CysHis: 0.262 ± 0.016
0.392CysIle: 0.392 ± 0.019
0.215CysLys: 0.215 ± 0.014
0.898CysLeu: 0.898 ± 0.034
0.159CysMet: 0.159 ± 0.012
0.253CysAsn: 0.253 ± 0.017
0.381CysPro: 0.381 ± 0.023
0.381CysGln: 0.381 ± 0.02
0.547CysArg: 0.547 ± 0.023
0.489CysSer: 0.489 ± 0.021
0.39CysThr: 0.39 ± 0.02
0.548CysVal: 0.548 ± 0.025
0.126CysTrp: 0.126 ± 0.01
0.265CysTyr: 0.265 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
6.627AspAla: 6.627 ± 0.091
0.521AspCys: 0.521 ± 0.022
4.398AspAsp: 4.398 ± 0.08
4.061AspGlu: 4.061 ± 0.063
2.412AspPhe: 2.412 ± 0.049
4.897AspGly: 4.897 ± 0.09
1.444AspHis: 1.444 ± 0.039
3.483AspIle: 3.483 ± 0.064
1.68AspLys: 1.68 ± 0.044
6.287AspLeu: 6.287 ± 0.092
1.312AspMet: 1.312 ± 0.034
2.085AspAsn: 2.085 ± 0.046
2.747AspPro: 2.747 ± 0.053
2.955AspGln: 2.955 ± 0.054
3.805AspArg: 3.805 ± 0.069
3.371AspSer: 3.371 ± 0.058
2.862AspThr: 2.862 ± 0.042
4.254AspVal: 4.254 ± 0.072
1.428AspTrp: 1.428 ± 0.043
2.078AspTyr: 2.078 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
6.549GluAla: 6.549 ± 0.088
0.38GluCys: 0.38 ± 0.019
2.92GluAsp: 2.92 ± 0.056
3.057GluGlu: 3.057 ± 0.063
2.016GluPhe: 2.016 ± 0.043
3.58GluGly: 3.58 ± 0.058
1.588GluHis: 1.588 ± 0.038
2.703GluIle: 2.703 ± 0.054
1.922GluLys: 1.922 ± 0.046
6.873GluLeu: 6.873 ± 0.086
1.415GluMet: 1.415 ± 0.041
1.906GluAsn: 1.906 ± 0.049
2.853GluPro: 2.853 ± 0.074
3.682GluGln: 3.682 ± 0.073
4.937GluArg: 4.937 ± 0.071
3.297GluSer: 3.297 ± 0.06
3.251GluThr: 3.251 ± 0.06
4.216GluVal: 4.216 ± 0.067
0.745GluTrp: 0.745 ± 0.026
1.341GluTyr: 1.341 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.545PheAla: 3.545 ± 0.059
0.427PheCys: 0.427 ± 0.018
2.774PheAsp: 2.774 ± 0.045
2.456PheGlu: 2.456 ± 0.047
1.599PhePhe: 1.599 ± 0.046
3.214PheGly: 3.214 ± 0.062
0.788PheHis: 0.788 ± 0.026
2.055PheIle: 2.055 ± 0.055
1.185PheLys: 1.185 ± 0.033
3.312PheLeu: 3.312 ± 0.064
0.892PheMet: 0.892 ± 0.027
1.671PheAsn: 1.671 ± 0.045
1.408PhePro: 1.408 ± 0.039
1.379PheGln: 1.379 ± 0.038
1.968PheArg: 1.968 ± 0.043
2.549PheSer: 2.549 ± 0.052
1.952PheThr: 1.952 ± 0.041
2.57PheVal: 2.57 ± 0.046
0.64PheTrp: 0.64 ± 0.029
1.204PheTyr: 1.204 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
6.363GlyAla: 6.363 ± 0.088
0.81GlyCys: 0.81 ± 0.024
4.55GlyAsp: 4.55 ± 0.092
4.594GlyGlu: 4.594 ± 0.062
3.441GlyPhe: 3.441 ± 0.053
5.612GlyGly: 5.612 ± 0.102
1.736GlyHis: 1.736 ± 0.041
4.199GlyIle: 4.199 ± 0.067
2.561GlyLys: 2.561 ± 0.055
8.558GlyLeu: 8.558 ± 0.108
1.99GlyMet: 1.99 ± 0.051
2.373GlyAsn: 2.373 ± 0.069
2.356GlyPro: 2.356 ± 0.042
3.538GlyGln: 3.538 ± 0.064
4.651GlyArg: 4.651 ± 0.072
4.26GlySer: 4.26 ± 0.075
3.612GlyThr: 3.612 ± 0.074
5.593GlyVal: 5.593 ± 0.075
1.337GlyTrp: 1.337 ± 0.038
2.494GlyTyr: 2.494 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
1.725HisAla: 1.725 ± 0.041
0.339HisCys: 0.339 ± 0.019
1.265HisAsp: 1.265 ± 0.037
1.096HisGlu: 1.096 ± 0.034
1.058HisPhe: 1.058 ± 0.034
1.602HisGly: 1.602 ± 0.04
0.737HisHis: 0.737 ± 0.03
1.026HisIle: 1.026 ± 0.031
0.591HisLys: 0.591 ± 0.022
2.614HisLeu: 2.614 ± 0.053
0.416HisMet: 0.416 ± 0.02
0.745HisAsn: 0.745 ± 0.024
1.403HisPro: 1.403 ± 0.039
1.374HisGln: 1.374 ± 0.037
1.704HisArg: 1.704 ± 0.041
1.364HisSer: 1.364 ± 0.033
0.984HisThr: 0.984 ± 0.032
1.003HisVal: 1.003 ± 0.034
0.592HisTrp: 0.592 ± 0.028
0.908HisTyr: 0.908 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
5.619IleAla: 5.619 ± 0.07
0.436IleCys: 0.436 ± 0.02
4.042IleAsp: 4.042 ± 0.065
3.925IleGlu: 3.925 ± 0.065
1.584IlePhe: 1.584 ± 0.048
4.229IleGly: 4.229 ± 0.071
1.072IleHis: 1.072 ± 0.028
2.394IleIle: 2.394 ± 0.054
1.706IleLys: 1.706 ± 0.041
4.596IleLeu: 4.596 ± 0.072
0.916IleMet: 0.916 ± 0.029
2.059IleAsn: 2.059 ± 0.049
2.173IlePro: 2.173 ± 0.051
2.001IleGln: 2.001 ± 0.042
3.173IleArg: 3.173 ± 0.05
3.074IleSer: 3.074 ± 0.053
2.742IleThr: 2.742 ± 0.051
3.363IleVal: 3.363 ± 0.057
0.649IleTrp: 0.649 ± 0.023
1.209IleTyr: 1.209 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.503LysAla: 3.503 ± 0.067
0.137LysCys: 0.137 ± 0.013
1.666LysAsp: 1.666 ± 0.041
1.592LysGlu: 1.592 ± 0.047
0.664LysPhe: 0.664 ± 0.025
2.057LysGly: 2.057 ± 0.05
0.664LysHis: 0.664 ± 0.026
1.402LysIle: 1.402 ± 0.042
1.306LysLys: 1.306 ± 0.051
3.169LysLeu: 3.169 ± 0.053
0.638LysMet: 0.638 ± 0.027
0.949LysAsn: 0.949 ± 0.03
1.749LysPro: 1.749 ± 0.041
1.361LysGln: 1.361 ± 0.038
2.219LysArg: 2.219 ± 0.045
1.632LysSer: 1.632 ± 0.047
1.975LysThr: 1.975 ± 0.046
2.286LysVal: 2.286 ± 0.044
0.277LysTrp: 0.277 ± 0.016
0.65LysTyr: 0.65 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
13.16LeuAla: 13.16 ± 0.142
1.002LeuCys: 1.002 ± 0.031
7.334LeuAsp: 7.334 ± 0.102
6.236LeuGlu: 6.236 ± 0.076
3.928LeuPhe: 3.928 ± 0.069
8.295LeuGly: 8.295 ± 0.099
2.251LeuHis: 2.251 ± 0.047
5.921LeuIle: 5.921 ± 0.097
3.801LeuLys: 3.801 ± 0.063
11.83LeuLeu: 11.83 ± 0.176
2.94LeuMet: 2.94 ± 0.063
4.485LeuAsn: 4.485 ± 0.07
5.674LeuPro: 5.674 ± 0.092
4.116LeuGln: 4.116 ± 0.068
6.049LeuArg: 6.049 ± 0.079
7.896LeuSer: 7.896 ± 0.099
6.541LeuThr: 6.541 ± 0.099
8.092LeuVal: 8.092 ± 0.104
1.51LeuTrp: 1.51 ± 0.046
2.715LeuTyr: 2.715 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
2.703MetAla: 2.703 ± 0.052
0.115MetCys: 0.115 ± 0.012
1.285MetAsp: 1.285 ± 0.033
1.106MetGlu: 1.106 ± 0.033
0.629MetPhe: 0.629 ± 0.025
1.642MetGly: 1.642 ± 0.044
0.452MetHis: 0.452 ± 0.021
1.272MetIle: 1.272 ± 0.032
0.938MetLys: 0.938 ± 0.029
2.511MetLeu: 2.511 ± 0.056
0.66MetMet: 0.66 ± 0.023
0.962MetAsn: 0.962 ± 0.029
1.238MetPro: 1.238 ± 0.033
0.977MetGln: 0.977 ± 0.031
1.364MetArg: 1.364 ± 0.035
1.755MetSer: 1.755 ± 0.038
1.757MetThr: 1.757 ± 0.038
1.706MetVal: 1.706 ± 0.04
0.157MetTrp: 0.157 ± 0.012
0.359MetTyr: 0.359 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.548AsnAla: 3.548 ± 0.065
0.269AsnCys: 0.269 ± 0.017
2.225AsnAsp: 2.225 ± 0.048
1.955AsnGlu: 1.955 ± 0.049
1.107AsnPhe: 1.107 ± 0.039
2.773AsnGly: 2.773 ± 0.067
0.786AsnHis: 0.786 ± 0.025
1.748AsnIle: 1.748 ± 0.039
0.97AsnLys: 0.97 ± 0.034
3.537AsnLeu: 3.537 ± 0.064
0.657AsnMet: 0.657 ± 0.025
1.301AsnAsn: 1.301 ± 0.039
1.961AsnPro: 1.961 ± 0.038
1.577AsnGln: 1.577 ± 0.038
2.258AsnArg: 2.258 ± 0.05
1.886AsnSer: 1.886 ± 0.049
1.825AsnThr: 1.825 ± 0.046
2.054AsnVal: 2.054 ± 0.056
0.577AsnTrp: 0.577 ± 0.023
0.973AsnTyr: 0.973 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
4.481ProAla: 4.481 ± 0.07
0.245ProCys: 0.245 ± 0.015
3.299ProAsp: 3.299 ± 0.053
3.36ProGlu: 3.36 ± 0.074
1.737ProPhe: 1.737 ± 0.039
3.432ProGly: 3.432 ± 0.06
0.89ProHis: 0.89 ± 0.03
2.193ProIle: 2.193 ± 0.045
1.356ProLys: 1.356 ± 0.042
4.944ProLeu: 4.944 ± 0.074
1.064ProMet: 1.064 ± 0.033
1.563ProAsn: 1.563 ± 0.042
1.655ProPro: 1.655 ± 0.038
1.87ProGln: 1.87 ± 0.047
2.011ProArg: 2.011 ± 0.044
2.688ProSer: 2.688 ± 0.047
2.375ProThr: 2.375 ± 0.041
3.634ProVal: 3.634 ± 0.061
0.767ProTrp: 0.767 ± 0.03
1.212ProTyr: 1.212 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
5.645GlnAla: 5.645 ± 0.084
0.371GlnCys: 0.371 ± 0.017
1.917GlnAsp: 1.917 ± 0.048
1.902GlnGlu: 1.902 ± 0.045
1.541GlnPhe: 1.541 ± 0.04
3.175GlnGly: 3.175 ± 0.052
1.239GlnHis: 1.239 ± 0.036
2.0GlnIle: 2.0 ± 0.044
1.149GlnLys: 1.149 ± 0.036
5.971GlnLeu: 5.971 ± 0.082
1.07GlnMet: 1.07 ± 0.032
1.3GlnAsn: 1.3 ± 0.037
2.579GlnPro: 2.579 ± 0.057
3.237GlnGln: 3.237 ± 0.057
3.805GlnArg: 3.805 ± 0.071
2.857GlnSer: 2.857 ± 0.051
2.445GlnThr: 2.445 ± 0.05
3.233GlnVal: 3.233 ± 0.055
0.97GlnTrp: 0.97 ± 0.034
1.132GlnTyr: 1.132 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
5.531ArgAla: 5.531 ± 0.072
0.512ArgCys: 0.512 ± 0.021
3.605ArgAsp: 3.605 ± 0.06
3.614ArgGlu: 3.614 ± 0.064
3.01ArgPhe: 3.01 ± 0.057
3.509ArgGly: 3.509 ± 0.063
1.706ArgHis: 1.706 ± 0.038
3.465ArgIle: 3.465 ± 0.059
1.837ArgLys: 1.837 ± 0.04
8.296ArgLeu: 8.296 ± 0.096
1.472ArgMet: 1.472 ± 0.038
1.917ArgAsn: 1.917 ± 0.04
2.545ArgPro: 2.545 ± 0.049
3.542ArgGln: 3.542 ± 0.06
4.248ArgArg: 4.248 ± 0.07
3.525ArgSer: 3.525 ± 0.06
2.872ArgThr: 2.872 ± 0.053
4.116ArgVal: 4.116 ± 0.069
1.169ArgTrp: 1.169 ± 0.034
2.168ArgTyr: 2.168 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
5.987SerAla: 5.987 ± 0.07
0.384SerCys: 0.384 ± 0.019
3.916SerAsp: 3.916 ± 0.061
3.427SerGlu: 3.427 ± 0.063
2.228SerPhe: 2.228 ± 0.044
5.382SerGly: 5.382 ± 0.1
1.329SerHis: 1.329 ± 0.035
3.035SerIle: 3.035 ± 0.059
1.648SerLys: 1.648 ± 0.041
6.92SerLeu: 6.92 ± 0.096
1.476SerMet: 1.476 ± 0.036
2.019SerAsn: 2.019 ± 0.05
2.44SerPro: 2.44 ± 0.049
2.77SerGln: 2.77 ± 0.054
3.572SerArg: 3.572 ± 0.056
3.519SerSer: 3.519 ± 0.082
3.0SerThr: 3.0 ± 0.06
4.499SerVal: 4.499 ± 0.062
0.9SerTrp: 0.9 ± 0.029
1.476SerTyr: 1.476 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
5.416ThrAla: 5.416 ± 0.078
0.35ThrCys: 0.35 ± 0.017
3.515ThrAsp: 3.515 ± 0.061
3.23ThrGlu: 3.23 ± 0.058
1.841ThrPhe: 1.841 ± 0.045
4.391ThrGly: 4.391 ± 0.079
1.123ThrHis: 1.123 ± 0.031
2.393ThrIle: 2.393 ± 0.048
1.091ThrLys: 1.091 ± 0.033
7.181ThrLeu: 7.181 ± 0.09
0.946ThrMet: 0.946 ± 0.029
1.527ThrAsn: 1.527 ± 0.039
2.809ThrPro: 2.809 ± 0.053
2.304ThrGln: 2.304 ± 0.045
2.801ThrArg: 2.801 ± 0.048
2.78ThrSer: 2.78 ± 0.053
2.872ThrThr: 2.872 ± 0.067
3.895ThrVal: 3.895 ± 0.063
0.67ThrTrp: 0.67 ± 0.025
1.152ThrTyr: 1.152 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
7.082ValAla: 7.082 ± 0.091
0.642ValCys: 0.642 ± 0.024
4.375ValAsp: 4.375 ± 0.063
4.406ValGlu: 4.406 ± 0.075
2.858ValPhe: 2.858 ± 0.055
5.019ValGly: 5.019 ± 0.073
1.427ValHis: 1.427 ± 0.038
4.069ValIle: 4.069 ± 0.066
2.061ValLys: 2.061 ± 0.052
7.811ValLeu: 7.811 ± 0.103
1.898ValMet: 1.898 ± 0.043
2.375ValAsn: 2.375 ± 0.05
3.017ValPro: 3.017 ± 0.059
2.705ValGln: 2.705 ± 0.05
4.25ValArg: 4.25 ± 0.067
4.577ValSer: 4.577 ± 0.073
4.108ValThr: 4.108 ± 0.074
5.501ValVal: 5.501 ± 0.08
0.982ValTrp: 0.982 ± 0.036
1.725ValTyr: 1.725 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
1.165TrpAla: 1.165 ± 0.035
0.166TrpCys: 0.166 ± 0.011
0.652TrpAsp: 0.652 ± 0.022
0.586TrpGlu: 0.586 ± 0.026
0.649TrpPhe: 0.649 ± 0.023
0.881TrpGly: 0.881 ± 0.028
0.459TrpHis: 0.459 ± 0.022
0.742TrpIle: 0.742 ± 0.028
0.311TrpLys: 0.311 ± 0.018
2.627TrpLeu: 2.627 ± 0.058
0.406TrpMet: 0.406 ± 0.02
0.458TrpAsn: 0.458 ± 0.023
0.835TrpPro: 0.835 ± 0.032
1.249TrpGln: 1.249 ± 0.042
1.135TrpArg: 1.135 ± 0.037
0.905TrpSer: 0.905 ± 0.03
0.661TrpThr: 0.661 ± 0.023
1.238TrpVal: 1.238 ± 0.039
0.248TrpTrp: 0.248 ± 0.017
0.362TrpTyr: 0.362 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.219TyrAla: 2.219 ± 0.04
0.297TyrCys: 0.297 ± 0.016
1.65TyrAsp: 1.65 ± 0.043
1.389TyrGlu: 1.389 ± 0.038
1.12TyrPhe: 1.12 ± 0.029
2.13TyrGly: 2.13 ± 0.048
0.656TyrHis: 0.656 ± 0.025
1.116TyrIle: 1.116 ± 0.032
0.661TyrLys: 0.661 ± 0.027
3.13TyrLeu: 3.13 ± 0.065
0.467TyrMet: 0.467 ± 0.021
0.863TyrAsn: 0.863 ± 0.032
1.284TyrPro: 1.284 ± 0.036
1.703TyrGln: 1.703 ± 0.044
2.228TyrArg: 2.228 ± 0.053
1.648TyrSer: 1.648 ± 0.037
1.19TyrThr: 1.19 ± 0.037
1.535TyrVal: 1.535 ± 0.037
0.526TyrTrp: 0.526 ± 0.024
0.828TyrTyr: 0.828 ± 0.03
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3452 proteins (1122755 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski