Caulobacter sp. 17J65-9
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4292 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B3UKC1|A0A6B3UKC1_9CAUL Uncharacterized protein (Fragment) OS=Caulobacter sp. 17J65-9 OX=2709382 GN=G3573_14915 PE=4 SV=1
MM1 pKa = 7.29 SSRR4 pKa = 11.84 RR5 pKa = 11.84 GVLLLGSALGVSIAVLAAAVPNRR28 pKa = 11.84 ALAADD33 pKa = 3.59 EE34 pKa = 4.88 CGAVPAGATPTVTCTVADD52 pKa = 3.7 SPYY55 pKa = 10.6 PGVQYY60 pKa = 11.08 DD61 pKa = 3.9 GVEE64 pKa = 4.16 DD65 pKa = 3.88 LTVVIQPDD73 pKa = 3.73 VTVEE77 pKa = 3.75 GLVRR81 pKa = 11.84 VTGTGDD87 pKa = 3.54 LAVNAQAGSSIDD99 pKa = 3.6 NPTGAGIQVTSSGTGSVTAASVSAGYY125 pKa = 10.88 GDD127 pKa = 4.07 GVSVSGEE134 pKa = 3.52 AGATVNVGTVSGDD147 pKa = 3.12 WTGVAASSNLGSVTVTSTSVTAVEE171 pKa = 4.37 GRR173 pKa = 11.84 GIFAWARR180 pKa = 11.84 AEE182 pKa = 4.09 GEE184 pKa = 4.51 TINITSGTVEE194 pKa = 4.38 SYY196 pKa = 11.51 DD197 pKa = 3.5 EE198 pKa = 4.94 GIYY201 pKa = 10.29 AYY203 pKa = 10.42 SGEE206 pKa = 4.35 GGSADD211 pKa = 3.48 VVVDD215 pKa = 3.71 SATVTTTRR223 pKa = 11.84 SSWATGIVAAASHH236 pKa = 6.8 GDD238 pKa = 3.42 VTVTSGSVTTAGDD251 pKa = 3.61 YY252 pKa = 11.29 SEE254 pKa = 5.82 GILVKK259 pKa = 10.3 AAEE262 pKa = 4.48 GEE264 pKa = 4.62 VYY266 pKa = 10.35 VQSDD270 pKa = 4.15 TVHH273 pKa = 5.97 TAGDD277 pKa = 3.88 YY278 pKa = 8.64 STGIKK283 pKa = 10.28 VEE285 pKa = 4.32 GGLGPVEE292 pKa = 4.07 LHH294 pKa = 6.56 SGSITTDD301 pKa = 3.13 GASASGIDD309 pKa = 3.64 VYY311 pKa = 8.93 TTSGAVTVTSDD322 pKa = 4.07 EE323 pKa = 3.93 IALNGGRR330 pKa = 11.84 AVEE333 pKa = 4.31 TTYY336 pKa = 11.49 GEE338 pKa = 4.26 YY339 pKa = 10.96 GSYY342 pKa = 7.09 TTYY345 pKa = 10.62 GRR347 pKa = 11.84 RR348 pKa = 11.84 PVGIDD353 pKa = 3.09 VRR355 pKa = 11.84 STDD358 pKa = 3.34 GAVTITSGDD367 pKa = 3.17 ISVTDD372 pKa = 4.66 GIGISVEE379 pKa = 3.99 TAGNISITSTGTLLVADD396 pKa = 4.63 GEE398 pKa = 4.79 RR399 pKa = 11.84 NGGYY403 pKa = 10.01 GYY405 pKa = 11.05 SSGIYY410 pKa = 9.82 FDD412 pKa = 4.18 SQNSGEE418 pKa = 4.4 GEE420 pKa = 3.91 PGGTVQVVNEE430 pKa = 4.0 GRR432 pKa = 11.84 IEE434 pKa = 3.74 IHH436 pKa = 5.75 GSNRR440 pKa = 11.84 NGIYY444 pKa = 10.4 GYY446 pKa = 10.95 GSGSAVSITNAGEE459 pKa = 3.98 IVGDD463 pKa = 3.81 RR464 pKa = 11.84 DD465 pKa = 3.28 TTAIRR470 pKa = 11.84 VHH472 pKa = 6.93 NEE474 pKa = 3.42 DD475 pKa = 3.58 GGALSVTNQAGASIHH490 pKa = 6.96 LYY492 pKa = 9.02 DD493 pKa = 3.97 TYY495 pKa = 11.13 STGIHH500 pKa = 6.68 AGTDD504 pKa = 3.22 EE505 pKa = 4.51 GALTITNAGSIVTEE519 pKa = 4.13 GDD521 pKa = 3.3 YY522 pKa = 11.35 SSAITFHH529 pKa = 7.52 SEE531 pKa = 3.46 AGTVTLNSTGTIEE544 pKa = 4.02 TSGYY548 pKa = 10.36 GSGAIYY554 pKa = 9.8 GHH556 pKa = 6.45 SYY558 pKa = 11.08 DD559 pKa = 4.15 GDD561 pKa = 3.91 VLVDD565 pKa = 3.45 VASVHH570 pKa = 6.07 VGGGSASGIDD580 pKa = 3.48 VWSEE584 pKa = 3.92 SGSVRR589 pKa = 11.84 VNADD593 pKa = 3.37 LVVTGSSCSEE603 pKa = 4.04 GEE605 pKa = 4.38 GCSGEE610 pKa = 4.1 GDD612 pKa = 3.92 SVGVSASSGTGNVTIDD628 pKa = 3.49 VGSVTTGDD636 pKa = 3.9 DD637 pKa = 3.61 YY638 pKa = 12.01 AVGVSAGMYY647 pKa = 10.43 GGFLGDD653 pKa = 3.63 AYY655 pKa = 10.79 SGEE658 pKa = 4.21 GEE660 pKa = 4.59 YY661 pKa = 11.09 VFGDD665 pKa = 3.39 IQVTADD671 pKa = 3.29 RR672 pKa = 11.84 VTTSGDD678 pKa = 2.81 WAAGIQVISGRR689 pKa = 11.84 DD690 pKa = 3.6 EE691 pKa = 4.6 YY692 pKa = 10.94 ISGPFGPPPIVRR704 pKa = 11.84 TGAVDD709 pKa = 3.59 IDD711 pKa = 3.6 AGTIVTHH718 pKa = 6.6 GSGSAGISVAAGSAEE733 pKa = 3.94 DD734 pKa = 4.85 VLGFWSGPMLGGEE747 pKa = 4.13 GGVNPFALVMGPHH760 pKa = 7.1 FGTTGDD766 pKa = 3.19 VDD768 pKa = 3.75 VDD770 pKa = 4.21 VVSVSTDD777 pKa = 2.52 GDD779 pKa = 4.04 GAVGVYY785 pKa = 10.16 ASSGFTLGFYY795 pKa = 11.29 GEE797 pKa = 4.53 GEE799 pKa = 4.26 YY800 pKa = 11.14 GGAEE804 pKa = 3.95 PGGDD808 pKa = 3.48 VIVTSGAIEE817 pKa = 4.0 TAGDD821 pKa = 3.64 GAIGIVARR829 pKa = 11.84 GVAGGVTIDD838 pKa = 3.53 SGTIEE843 pKa = 4.11 TSGDD847 pKa = 3.58 YY848 pKa = 11.05 ASGIVANNGGVVFTRR863 pKa = 11.84 VEE865 pKa = 4.09 IEE867 pKa = 3.88 PEE869 pKa = 4.24 FYY871 pKa = 8.38 TTVPSFVSGGLIDD884 pKa = 3.74 VTSDD888 pKa = 3.17 KK889 pKa = 10.61 IYY891 pKa = 10.73 TSGEE895 pKa = 3.81 SSAGITAVGMGGGVTIDD912 pKa = 3.66 SGIIDD917 pKa = 3.44 TWGAYY922 pKa = 10.33 ANGIQVMAIGGDD934 pKa = 3.46 VSVDD938 pKa = 3.15 STRR941 pKa = 11.84 IIARR945 pKa = 11.84 GDD947 pKa = 3.13 RR948 pKa = 11.84 SGGILINTANLDD960 pKa = 3.72 SFFGEE965 pKa = 4.52 GEE967 pKa = 4.03 YY968 pKa = 11.08 GATFVAGDD976 pKa = 3.19 IHH978 pKa = 5.87 VTSGTIRR985 pKa = 11.84 TEE987 pKa = 3.98 GGSADD992 pKa = 4.24 GIAVFSGYY1000 pKa = 11.25 AFFGEE1005 pKa = 4.63 GEE1007 pKa = 4.26 YY1008 pKa = 11.21 GGGDD1012 pKa = 3.34 VDD1014 pKa = 3.56 ITSGNITTLGDD1025 pKa = 3.44 DD1026 pKa = 3.5 SAGIRR1031 pKa = 11.84 VTAYY1035 pKa = 10.22 GRR1037 pKa = 11.84 YY1038 pKa = 7.93 TYY1040 pKa = 11.23 GDD1042 pKa = 3.62 DD1043 pKa = 3.87 EE1044 pKa = 4.38 SWEE1047 pKa = 3.98 IEE1049 pKa = 3.96 GDD1051 pKa = 3.89 VTVHH1055 pKa = 6.04 SGAVITSGDD1064 pKa = 3.74 YY1065 pKa = 10.56 SDD1067 pKa = 5.59 GIVVEE1072 pKa = 4.28 ATLGDD1077 pKa = 3.91 VNVTANATATSGDD1090 pKa = 3.51 SSRR1093 pKa = 11.84 GIDD1096 pKa = 3.05 IDD1098 pKa = 4.02 ADD1100 pKa = 3.68 NGDD1103 pKa = 3.76 VVVNATAVTTTGGRR1117 pKa = 11.84 KK1118 pKa = 9.37 ANEE1121 pKa = 3.42 VSYY1124 pKa = 10.67 YY1125 pKa = 10.95 DD1126 pKa = 4.06 SYY1128 pKa = 10.51 TEE1130 pKa = 3.51 SWVYY1134 pKa = 7.95 VTEE1137 pKa = 4.2 YY1138 pKa = 10.92 GHH1140 pKa = 7.07 ASDD1143 pKa = 5.49 GIRR1146 pKa = 11.84 VEE1148 pKa = 4.24 TEE1150 pKa = 3.79 YY1151 pKa = 11.78 GDD1153 pKa = 3.7 VTINAQNVTVSGDD1166 pKa = 3.12 QATGVLVDD1174 pKa = 4.12 SEE1176 pKa = 4.52 YY1177 pKa = 11.66 GDD1179 pKa = 3.41 VKK1181 pKa = 11.25 LNLGSVRR1188 pKa = 11.84 AQGEE1192 pKa = 3.99 AGDD1195 pKa = 4.14 AVHH1198 pKa = 6.71 VYY1200 pKa = 10.19 AYY1202 pKa = 10.14 EE1203 pKa = 4.09 GDD1205 pKa = 3.23 IGVNISGQVTSANGAGVHH1223 pKa = 6.39 AYY1225 pKa = 9.8 SYY1227 pKa = 11.15 YY1228 pKa = 10.93 GGVDD1232 pKa = 3.27 VKK1234 pKa = 10.68 LAAGSRR1240 pKa = 11.84 VQGGTVGIYY1249 pKa = 9.98 AYY1251 pKa = 10.48 SDD1253 pKa = 3.11 TGTTITINGTVSAASGLAIDD1273 pKa = 4.46 VKK1275 pKa = 11.19 GLGAATIHH1283 pKa = 6.03 NLSNTVIGGVSLTDD1297 pKa = 3.76 NDD1299 pKa = 5.06 DD1300 pKa = 4.28 LFDD1303 pKa = 3.75 NTGTFQATIDD1313 pKa = 3.77 SQFGEE1318 pKa = 4.62 GVDD1321 pKa = 4.59 LFDD1324 pKa = 4.51 NTGLLQILPGSSTPGTIVFQGLEE1347 pKa = 4.1 TFDD1350 pKa = 3.55 NAGVVDD1356 pKa = 5.02 LANGHH1361 pKa = 6.61 AGDD1364 pKa = 4.29 VFDD1367 pKa = 6.09 LSSATWNGEE1376 pKa = 3.82 AGSTLEE1382 pKa = 4.67 LDD1384 pKa = 3.55 ISGSGSDD1391 pKa = 3.53 TLLVDD1396 pKa = 4.44 DD1397 pKa = 4.9 ATGHH1401 pKa = 4.52 TTIVLSGAGGGIGQALEE1418 pKa = 4.37 LVVSNSDD1425 pKa = 3.15 EE1426 pKa = 4.25 TGNEE1430 pKa = 3.69 FTLDD1434 pKa = 3.34 GDD1436 pKa = 3.78 TGFVKK1441 pKa = 10.81 FIMDD1445 pKa = 3.87 FDD1447 pKa = 4.51 AATDD1451 pKa = 3.21 TWSAVGTADD1460 pKa = 3.69 TEE1462 pKa = 4.33 VFEE1465 pKa = 5.03 PGRR1468 pKa = 11.84 FLTGAQNFWNEE1479 pKa = 4.02 TGDD1482 pKa = 3.2 AWSARR1487 pKa = 11.84 MTEE1490 pKa = 4.01 LRR1492 pKa = 11.84 DD1493 pKa = 3.26 IGSDD1497 pKa = 3.09 RR1498 pKa = 11.84 GQGAQGWVQAFGGEE1512 pKa = 4.06 QSEE1515 pKa = 4.46 DD1516 pKa = 3.07 RR1517 pKa = 11.84 GTEE1520 pKa = 3.64 RR1521 pKa = 11.84 FTVGGAPSDD1530 pKa = 3.67 VNLAYY1535 pKa = 9.43 EE1536 pKa = 3.7 QSYY1539 pKa = 10.84 RR1540 pKa = 11.84 GLQTGVDD1547 pKa = 3.72 WSTGDD1552 pKa = 3.27 TVLGVTIGFGDD1563 pKa = 3.65 SEE1565 pKa = 4.25 QRR1567 pKa = 11.84 MASGNSAEE1575 pKa = 4.0 FGGFNFGAYY1584 pKa = 9.89 GGWNPGSFFVNGLVKK1599 pKa = 10.54 ADD1601 pKa = 3.59 VYY1603 pKa = 10.02 KK1604 pKa = 10.68 TRR1606 pKa = 11.84 LGLRR1610 pKa = 11.84 TLQASEE1616 pKa = 4.25 EE1617 pKa = 4.24 VDD1619 pKa = 3.16 GRR1621 pKa = 11.84 TWGAKK1626 pKa = 9.9 AEE1628 pKa = 4.32 AGWRR1632 pKa = 11.84 VGGDD1636 pKa = 2.75 GWFFEE1641 pKa = 4.61 PSARR1645 pKa = 11.84 LSWTDD1650 pKa = 2.53 VDD1652 pKa = 3.68 MDD1654 pKa = 3.98 GFSAAGGTVEE1664 pKa = 5.76 FGDD1667 pKa = 3.82 ASSLLGEE1674 pKa = 4.25 AGARR1678 pKa = 11.84 FGTHH1682 pKa = 4.32 WTNGVVDD1689 pKa = 4.81 LVPYY1693 pKa = 10.6 LGLFAVDD1700 pKa = 4.49 EE1701 pKa = 4.59 FDD1703 pKa = 5.96 GEE1705 pKa = 4.27 NQTAFSIAGDD1715 pKa = 3.79 TFRR1718 pKa = 11.84 LTDD1721 pKa = 3.61 EE1722 pKa = 4.25 AAGAHH1727 pKa = 5.55 GRR1729 pKa = 11.84 VDD1731 pKa = 4.27 FGVTAVTFHH1740 pKa = 6.28 GLEE1743 pKa = 4.16 GFVKK1747 pKa = 10.91 GEE1749 pKa = 3.93 ALVGGDD1755 pKa = 3.53 AEE1757 pKa = 5.05 GYY1759 pKa = 8.32 SARR1762 pKa = 11.84 LGVRR1766 pKa = 11.84 WRR1768 pKa = 11.84 WW1769 pKa = 3.03
Molecular weight: 178.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.808
IPC_protein 3.846
Toseland 3.63
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.757
Rodwell 3.668
Grimsley 3.528
Solomon 3.834
Lehninger 3.783
Nozaki 3.935
DTASelect 4.177
Thurlkill 3.668
EMBOSS 3.757
Sillero 3.961
Patrickios 1.418
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.867
Protein with the highest isoelectric point:
>tr|A0A6B3USG0|A0A6B3USG0_9CAUL Alpha/beta hydrolase OS=Caulobacter sp. 17J65-9 OX=2709382 GN=G3573_16210 PE=4 SV=1
AA1 pKa = 7.78 SSAQSRR7 pKa = 11.84 SAQARR12 pKa = 11.84 GTRR15 pKa = 11.84 RR16 pKa = 11.84 WPPVIALSAAAAVSAGLWVAVVAAVRR42 pKa = 11.84 ALLL45 pKa = 3.85
Molecular weight: 4.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.386
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4292
0
4292
1247758
35
2282
290.7
31.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.464 ± 0.058
0.745 ± 0.014
5.869 ± 0.035
5.817 ± 0.04
3.554 ± 0.022
9.155 ± 0.051
1.737 ± 0.017
3.584 ± 0.022
3.063 ± 0.034
9.963 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.091 ± 0.017
2.134 ± 0.024
5.529 ± 0.032
2.975 ± 0.022
7.591 ± 0.039
4.786 ± 0.03
5.123 ± 0.042
8.106 ± 0.032
1.566 ± 0.017
2.146 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here