Mobiluncus mulieris ATCC 35239
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2379 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E0QPV6|E0QPV6_9ACTO DEAD/DEAH box helicase OS=Mobiluncus mulieris ATCC 35239 OX=871571 GN=HMPREF0580_0883 PE=4 SV=1
MM1 pKa = 8.04 SEE3 pKa = 4.45 EE4 pKa = 4.1 YY5 pKa = 10.16 TPQLLTSEE13 pKa = 5.17 DD14 pKa = 3.94 VFTVQFPATKK24 pKa = 10.14 FRR26 pKa = 11.84 DD27 pKa = 4.43 GYY29 pKa = 10.67 DD30 pKa = 3.03 QNQIDD35 pKa = 4.67 DD36 pKa = 4.11 YY37 pKa = 10.39 LDD39 pKa = 3.29 EE40 pKa = 4.38 VVRR43 pKa = 11.84 VLSYY47 pKa = 11.4 YY48 pKa = 9.85 EE49 pKa = 4.31 ALSAAPEE56 pKa = 4.22 AEE58 pKa = 3.8 VDD60 pKa = 3.41 LTYY63 pKa = 9.95 ITVRR67 pKa = 11.84 GVDD70 pKa = 3.47 VRR72 pKa = 11.84 EE73 pKa = 3.62 VDD75 pKa = 3.92 FDD77 pKa = 3.77 YY78 pKa = 11.3 TRR80 pKa = 11.84 MRR82 pKa = 11.84 VGYY85 pKa = 9.8 DD86 pKa = 2.92 QDD88 pKa = 4.8 AVDD91 pKa = 5.52 DD92 pKa = 4.27 YY93 pKa = 11.24 LDD95 pKa = 3.46 QVAATLEE102 pKa = 4.35 AYY104 pKa = 9.08 EE105 pKa = 4.16 NAYY108 pKa = 9.96 GIPPSDD114 pKa = 3.18 QRR116 pKa = 11.84 YY117 pKa = 8.28 QVLQVDD123 pKa = 5.36 GPALEE128 pKa = 5.45 AEE130 pKa = 4.7 AAQQAAAAGEE140 pKa = 4.2 LPAEE144 pKa = 4.37 VSAPVALGDD153 pKa = 4.09 SAAGGAPVTAGNMLSLGASVGGAVPLDD180 pKa = 4.1 AAPSAPDD187 pKa = 3.19 KK188 pKa = 10.87 AASLGASEE196 pKa = 4.63 TAPNSPGYY204 pKa = 10.39 SSVLGTPPARR214 pKa = 11.84 QPGVGEE220 pKa = 4.17 FNEE223 pKa = 4.33 APQYY227 pKa = 10.84 PGDD230 pKa = 4.0 SLDD233 pKa = 3.15 WMQAPGQSPASSPQNYY249 pKa = 8.69 PDD251 pKa = 4.1 YY252 pKa = 10.66 PPLYY256 pKa = 8.45 PQPDD260 pKa = 3.64 ADD262 pKa = 3.79 STTFGASSGYY272 pKa = 10.03 GFSPVAPGEE281 pKa = 4.14 YY282 pKa = 10.16 ASDD285 pKa = 3.9 YY286 pKa = 10.71 NDD288 pKa = 3.7 EE289 pKa = 4.23 EE290 pKa = 5.05 GEE292 pKa = 4.2 YY293 pKa = 10.97 ADD295 pKa = 4.73 YY296 pKa = 11.45 DD297 pKa = 3.88 GGNPNQDD304 pKa = 2.63 EE305 pKa = 4.48 SAAIPAEE312 pKa = 4.19 TTAPSGQANQGGAIPGYY329 pKa = 9.38 GFPAGSPTEE338 pKa = 4.46 SMAADD343 pKa = 3.95 MPDD346 pKa = 3.02 VDD348 pKa = 4.31 LTEE351 pKa = 5.85 DD352 pKa = 3.69 DD353 pKa = 4.85 GEE355 pKa = 4.67 TEE357 pKa = 4.18 AEE359 pKa = 4.11 LPGEE363 pKa = 4.13 IGLIEE368 pKa = 4.2 TEE370 pKa = 4.23 EE371 pKa = 3.96 ATGFDD376 pKa = 4.9 DD377 pKa = 5.89 FGEE380 pKa = 4.54 TPAAEE385 pKa = 4.2 MAPHH389 pKa = 7.02 EE390 pKa = 4.85 DD391 pKa = 3.74 AFPPPTDD398 pKa = 3.35 TPTPNPYY405 pKa = 10.19 PMEE408 pKa = 5.1 SGGYY412 pKa = 9.99 GNAPDD417 pKa = 5.03 PGFEE421 pKa = 5.11 AYY423 pKa = 9.19 GQPEE427 pKa = 3.63 AWGNDD432 pKa = 2.89 GSAADD437 pKa = 4.25 TTPGGYY443 pKa = 9.47 PEE445 pKa = 4.63 VPRR448 pKa = 11.84 DD449 pKa = 3.22 PNFAYY454 pKa = 10.02 GYY456 pKa = 8.3 AQPGAPAGDD465 pKa = 3.63 YY466 pKa = 10.78 GYY468 pKa = 10.6 QEE470 pKa = 3.77 QDD472 pKa = 2.44 GVYY475 pKa = 10.33 AEE477 pKa = 4.91 NSGEE481 pKa = 4.45 TNWEE485 pKa = 3.99 AMPQPGNDD493 pKa = 3.15 TPMPQGNNPYY503 pKa = 9.56 DD504 pKa = 3.9 APGSAQFEE512 pKa = 4.14 AAGYY516 pKa = 9.65 GDD518 pKa = 4.97 AYY520 pKa = 10.75 ANEE523 pKa = 4.12 AATGYY528 pKa = 8.38 EE529 pKa = 4.24 ANFGEE534 pKa = 5.07 DD535 pKa = 4.16 GYY537 pKa = 9.59 TEE539 pKa = 4.06 TGMAAAGGEE548 pKa = 4.75 TNGEE552 pKa = 4.25 TYY554 pKa = 10.73 NPSEE558 pKa = 4.13 AGQYY562 pKa = 9.74 PEE564 pKa = 4.07 YY565 pKa = 10.78 ADD567 pKa = 3.91 YY568 pKa = 11.28 PEE570 pKa = 4.6 TPGTNPDD577 pKa = 3.03 VSEE580 pKa = 4.51 FAVEE584 pKa = 4.57 TPFTPTDD591 pKa = 3.36 MSNGYY596 pKa = 7.55 QQYY599 pKa = 10.35 DD600 pKa = 3.81 AYY602 pKa = 9.4 PEE604 pKa = 4.02 QVSPEE609 pKa = 3.94 NGGTGEE615 pKa = 4.03 FAAGFGGDD623 pKa = 3.91 EE624 pKa = 4.82 GYY626 pKa = 11.08 SDD628 pKa = 3.57 ATGYY632 pKa = 11.18 DD633 pKa = 3.74 DD634 pKa = 6.1 DD635 pKa = 4.35 NAYY638 pKa = 8.27 PTEE641 pKa = 4.17 TAAGMGDD648 pKa = 3.17 AGGGDD653 pKa = 4.61 FEE655 pKa = 4.53 ATGYY659 pKa = 10.22 PEE661 pKa = 3.93 TNAEE665 pKa = 4.08 FTEE668 pKa = 4.41 DD669 pKa = 2.97 TGGFATPGWGTEE681 pKa = 4.32 TYY683 pKa = 10.32 PGTEE687 pKa = 4.45 PDD689 pKa = 3.3 TLAAGSADD697 pKa = 3.8 GLDD700 pKa = 3.83 SPAGGTTATAAVAATTPAFNLPGEE724 pKa = 4.58 APAVPPVDD732 pKa = 3.75 TPVEE736 pKa = 4.31 PIRR739 pKa = 11.84 NPFTPATGNAAGAVEE754 pKa = 4.31 SSGIAMPPHH763 pKa = 6.36 EE764 pKa = 5.34 PLTEE768 pKa = 4.13 VVTPDD773 pKa = 3.76 LPTPGVGLDD782 pKa = 3.6 LSSLKK787 pKa = 8.42 TTDD790 pKa = 3.62 SKK792 pKa = 10.34 TEE794 pKa = 4.0 PYY796 pKa = 10.2 SAGEE800 pKa = 4.15 APSGQAPHH808 pKa = 6.9 EE809 pKa = 4.61 PGEE812 pKa = 4.36 TNATGKK818 pKa = 10.32 FSAAALEE825 pKa = 4.56 ALDD828 pKa = 4.56 QARR831 pKa = 11.84 NDD833 pKa = 3.84 PDD835 pKa = 3.52 LGKK838 pKa = 10.64 ASLTNSVAAANQVASTASPNHH859 pKa = 5.78 KK860 pKa = 9.91 RR861 pKa = 11.84 FAEE864 pKa = 4.32 VTSRR868 pKa = 11.84 ATEE871 pKa = 4.09 TAAEE875 pKa = 4.15 TATVEE880 pKa = 4.12 NGRR883 pKa = 11.84 PDD885 pKa = 3.15 ATQPPKK891 pKa = 10.84 NLARR895 pKa = 11.84 NRR897 pKa = 11.84 GDD899 pKa = 3.31 KK900 pKa = 10.66 PGGGDD905 pKa = 3.44 TVTAAYY911 pKa = 7.74 PAGLAGIPGLEE922 pKa = 4.08 PLNPAEE928 pKa = 4.99 LLTPATGIEE937 pKa = 4.71 TVTGPTFTEE946 pKa = 4.25 VPIDD950 pKa = 3.56 HH951 pKa = 6.85 EE952 pKa = 4.32 AASRR956 pKa = 11.84 LAEE959 pKa = 4.09 TTDD962 pKa = 2.93 IDD964 pKa = 4.26 TADD967 pKa = 3.79 EE968 pKa = 4.2 TASTPRR974 pKa = 11.84 EE975 pKa = 4.06 TEE977 pKa = 3.87 EE978 pKa = 3.92 PSTPKK983 pKa = 10.21 EE984 pKa = 4.07 IPEE987 pKa = 4.13 PTPKK991 pKa = 10.27 EE992 pKa = 4.02 IPEE995 pKa = 4.09 PTPKK999 pKa = 9.42 EE1000 pKa = 4.46 TEE1002 pKa = 3.81 TSTFSGTKK1010 pKa = 10.12 LPEE1013 pKa = 4.24 NANPRR1018 pKa = 11.84 PLLQGEE1024 pKa = 4.45 EE1025 pKa = 4.22 PFGEE1029 pKa = 4.32 VTEE1032 pKa = 4.39 EE1033 pKa = 4.18 GRR1035 pKa = 11.84 FVPHH1039 pKa = 6.52 FLAGYY1044 pKa = 7.56 RR1045 pKa = 11.84 TNLDD1049 pKa = 3.81 TFTSAYY1055 pKa = 10.15 GSLEE1059 pKa = 4.08 EE1060 pKa = 5.09 HH1061 pKa = 6.25 SQPHH1065 pKa = 5.54 AKK1067 pKa = 10.09 KK1068 pKa = 9.7 PEE1070 pKa = 4.47 SIAKK1074 pKa = 10.23 LEE1076 pKa = 4.12 AEE1078 pKa = 4.46 QPRR1081 pKa = 11.84 KK1082 pKa = 10.48 SITTAYY1088 pKa = 9.9 LVTVTTSRR1096 pKa = 11.84 PLGSDD1101 pKa = 2.9 DD1102 pKa = 3.59 AVFVRR1107 pKa = 11.84 FPDD1110 pKa = 3.75 GRR1112 pKa = 11.84 EE1113 pKa = 3.93 LPVTSAYY1120 pKa = 11.02 SDD1122 pKa = 3.66 FNGVHH1127 pKa = 6.36 LNIPRR1132 pKa = 11.84 LL1133 pKa = 3.66
Molecular weight: 117.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.503
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.567
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.63
Nozaki 3.783
DTASelect 3.973
Thurlkill 3.541
EMBOSS 3.592
Sillero 3.821
Patrickios 0.68
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.732
Protein with the highest isoelectric point:
>tr|E0QN63|E0QN63_9ACTO Glycosyltransferase group 2 family protein OS=Mobiluncus mulieris ATCC 35239 OX=871571 GN=rfaG PE=4 SV=1
MM1 pKa = 7.62 TKK3 pKa = 9.14 RR4 pKa = 11.84 TYY6 pKa = 10.01 QPHH9 pKa = 5.09 NRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 10.24 KK16 pKa = 8.45 HH17 pKa = 4.25 GFRR20 pKa = 11.84 NRR22 pKa = 11.84 MATRR26 pKa = 11.84 AGRR29 pKa = 11.84 AVISARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 GRR40 pKa = 11.84 KK41 pKa = 8.5 QLAVV45 pKa = 3.36
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2379
0
2379
730285
37
7651
307.0
33.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.052 ± 0.062
0.876 ± 0.016
5.253 ± 0.043
5.915 ± 0.049
3.421 ± 0.034
8.223 ± 0.054
1.973 ± 0.023
5.022 ± 0.047
4.28 ± 0.078
9.844 ± 0.084
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.269 ± 0.029
3.286 ± 0.041
5.157 ± 0.048
3.693 ± 0.028
5.88 ± 0.063
6.032 ± 0.041
6.126 ± 0.055
7.847 ± 0.048
1.439 ± 0.024
2.41 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here