Amino acid dipepetide frequency for Clostridium sp. CAG:568

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.098AlaAla: 3.098 ± 0.12	0.718AlaCys: 0.718 ± 0.038	3.431AlaAsp: 3.431 ± 0.089	3.102AlaGlu: 3.102 ± 0.106	2.826AlaPhe: 2.826 ± 0.088	3.285AlaGly: 3.285 ± 0.117	0.859AlaHis: 0.859 ± 0.048	4.739AlaIle: 4.739 ± 0.121	5.035AlaLys: 5.035 ± 0.123	5.958AlaLeu: 5.958 ± 0.13	1.662AlaMet: 1.662 ± 0.074	2.893AlaAsn: 2.893 ± 0.079	1.5AlaPro: 1.5 ± 0.066	1.593AlaGln: 1.593 ± 0.066	2.07AlaArg: 2.07 ± 0.079	4.064AlaSer: 4.064 ± 0.121	3.164AlaThr: 3.164 ± 0.113	3.677AlaVal: 3.677 ± 0.115	0.383AlaTrp: 0.383 ± 0.027	2.528AlaTyr: 2.528 ± 0.089	0.005AlaXaa: 0.005 ± 0.003
Cys	0.65CysAla: 0.65 ± 0.039	0.178CysCys: 0.178 ± 0.02	0.811CysAsp: 0.811 ± 0.039	0.581CysGlu: 0.581 ± 0.036	0.736CysPhe: 0.736 ± 0.039	0.962CysGly: 0.962 ± 0.046	0.255CysHis: 0.255 ± 0.023	0.837CysIle: 0.837 ± 0.05	0.686CysLys: 0.686 ± 0.043	1.313CysLeu: 1.313 ± 0.059	0.285CysMet: 0.285 ± 0.026	0.472CysAsn: 0.472 ± 0.032	0.515CysPro: 0.515 ± 0.035	0.312CysGln: 0.312 ± 0.026	0.353CysArg: 0.353 ± 0.032	0.848CysSer: 0.848 ± 0.04	0.57CysThr: 0.57 ± 0.035	0.682CysVal: 0.682 ± 0.039	0.114CysTrp: 0.114 ± 0.018	0.613CysTyr: 0.613 ± 0.04	0.0CysXaa: 0.0 ± 0.0
Asp	3.378AspAla: 3.378 ± 0.116	0.754AspCys: 0.754 ± 0.042	4.618AspAsp: 4.618 ± 0.11	5.363AspGlu: 5.363 ± 0.125	3.494AspPhe: 3.494 ± 0.095	4.967AspGly: 4.967 ± 0.133	0.88AspHis: 0.88 ± 0.049	5.147AspIle: 5.147 ± 0.12	5.758AspLys: 5.758 ± 0.112	5.765AspLeu: 5.765 ± 0.135	1.614AspMet: 1.614 ± 0.057	3.576AspAsn: 3.576 ± 0.104	2.038AspPro: 2.038 ± 0.084	1.536AspGln: 1.536 ± 0.069	2.213AspArg: 2.213 ± 0.089	5.083AspSer: 5.083 ± 0.116	3.127AspThr: 3.127 ± 0.087	4.114AspVal: 4.114 ± 0.102	0.606AspTrp: 0.606 ± 0.047	3.474AspTyr: 3.474 ± 0.104	0.0AspXaa: 0.0 ± 0.0
Glu	4.037GluAla: 4.037 ± 0.116	0.606GluCys: 0.606 ± 0.039	4.235GluAsp: 4.235 ± 0.107	5.149GluGlu: 5.149 ± 0.142	2.838GluPhe: 2.838 ± 0.078	3.827GluGly: 3.827 ± 0.097	0.818GluHis: 0.818 ± 0.045	5.106GluIle: 5.106 ± 0.116	6.396GluLys: 6.396 ± 0.138	5.375GluLeu: 5.375 ± 0.116	1.641GluMet: 1.641 ± 0.061	3.994GluAsn: 3.994 ± 0.104	1.575GluPro: 1.575 ± 0.073	1.691GluGln: 1.691 ± 0.07	2.286GluArg: 2.286 ± 0.081	3.706GluSer: 3.706 ± 0.101	3.264GluThr: 3.264 ± 0.088	3.745GluVal: 3.745 ± 0.091	0.517GluTrp: 0.517 ± 0.034	2.649GluTyr: 2.649 ± 0.075	0.0GluXaa: 0.0 ± 0.0
Phe	2.544PheAla: 2.544 ± 0.077	0.652PheCys: 0.652 ± 0.036	3.811PheAsp: 3.811 ± 0.109	2.758PheGlu: 2.758 ± 0.081	2.569PhePhe: 2.569 ± 0.102	3.276PheGly: 3.276 ± 0.104	0.686PheHis: 0.686 ± 0.038	4.021PheIle: 4.021 ± 0.12	3.886PheLys: 3.886 ± 0.103	4.235PheLeu: 4.235 ± 0.118	1.272PheMet: 1.272 ± 0.055	2.936PheAsn: 2.936 ± 0.077	1.361PhePro: 1.361 ± 0.058	1.156PheGln: 1.156 ± 0.05	1.345PheArg: 1.345 ± 0.069	3.875PheSer: 3.875 ± 0.115	2.583PheThr: 2.583 ± 0.091	2.938PheVal: 2.938 ± 0.086	0.365PheTrp: 0.365 ± 0.029	2.364PheTyr: 2.364 ± 0.07	0.0PheXaa: 0.0 ± 0.0
Gly	3.437GlyAla: 3.437 ± 0.127	0.807GlyCys: 0.807 ± 0.043	3.72GlyAsp: 3.72 ± 0.099	3.693GlyGlu: 3.693 ± 0.096	3.389GlyPhe: 3.389 ± 0.098	3.866GlyGly: 3.866 ± 0.138	1.222GlyHis: 1.222 ± 0.063	5.038GlyIle: 5.038 ± 0.123	5.516GlyLys: 5.516 ± 0.122	5.961GlyLeu: 5.961 ± 0.122	1.673GlyMet: 1.673 ± 0.067	3.458GlyAsn: 3.458 ± 0.097	1.158GlyPro: 1.158 ± 0.052	1.792GlyGln: 1.792 ± 0.072	2.273GlyArg: 2.273 ± 0.09	4.089GlySer: 4.089 ± 0.107	3.836GlyThr: 3.836 ± 0.125	4.108GlyVal: 4.108 ± 0.119	0.661GlyTrp: 0.661 ± 0.045	3.223GlyTyr: 3.223 ± 0.097	0.0GlyXaa: 0.0 ± 0.0
His	0.782HisAla: 0.782 ± 0.048	0.194HisCys: 0.194 ± 0.02	0.973HisAsp: 0.973 ± 0.045	0.839HisGlu: 0.839 ± 0.049	0.777HisPhe: 0.777 ± 0.046	1.012HisGly: 1.012 ± 0.052	0.278HisHis: 0.278 ± 0.026	1.117HisIle: 1.117 ± 0.06	1.023HisLys: 1.023 ± 0.049	1.233HisLeu: 1.233 ± 0.061	0.328HisMet: 0.328 ± 0.029	0.729HisAsn: 0.729 ± 0.047	0.686HisPro: 0.686 ± 0.042	0.431HisGln: 0.431 ± 0.033	0.59HisArg: 0.59 ± 0.039	0.978HisSer: 0.978 ± 0.05	0.727HisThr: 0.727 ± 0.044	0.823HisVal: 0.823 ± 0.053	0.144HisTrp: 0.144 ± 0.017	0.718HisTyr: 0.718 ± 0.042	0.0HisXaa: 0.0 ± 0.0
Ile	4.709IleAla: 4.709 ± 0.112	1.185IleCys: 1.185 ± 0.048	5.979IleAsp: 5.979 ± 0.135	5.11IleGlu: 5.11 ± 0.117	3.305IlePhe: 3.305 ± 0.102	5.149IleGly: 5.149 ± 0.129	1.197IleHis: 1.197 ± 0.055	5.842IleIle: 5.842 ± 0.148	6.738IleLys: 6.738 ± 0.153	6.667IleLeu: 6.667 ± 0.137	1.504IleMet: 1.504 ± 0.066	4.657IleAsn: 4.657 ± 0.118	2.874IlePro: 2.874 ± 0.09	2.097IleGln: 2.097 ± 0.078	2.633IleArg: 2.633 ± 0.086	5.988IleSer: 5.988 ± 0.13	4.436IleThr: 4.436 ± 0.128	5.357IleVal: 5.357 ± 0.116	0.504IleTrp: 0.504 ± 0.032	3.276IleTyr: 3.276 ± 0.101	0.0IleXaa: 0.0 ± 0.0
Lys	5.475LysAla: 5.475 ± 0.12	0.641LysCys: 0.641 ± 0.041	6.72LysAsp: 6.72 ± 0.126	7.508LysGlu: 7.508 ± 0.162	3.025LysPhe: 3.025 ± 0.09	4.855LysGly: 4.855 ± 0.106	1.128LysHis: 1.128 ± 0.055	6.437LysIle: 6.437 ± 0.142	8.489LysLys: 8.489 ± 0.164	6.15LysLeu: 6.15 ± 0.116	2.38LysMet: 2.38 ± 0.068	5.815LysAsn: 5.815 ± 0.124	2.633LysPro: 2.633 ± 0.087	2.131LysGln: 2.131 ± 0.084	2.947LysArg: 2.947 ± 0.098	5.441LysSer: 5.441 ± 0.112	4.996LysThr: 4.996 ± 0.12	5.487LysVal: 5.487 ± 0.113	0.581LysTrp: 0.581 ± 0.039	3.654LysTyr: 3.654 ± 0.104	0.002LysXaa: 0.002 ± 0.003
Leu	5.683LeuAla: 5.683 ± 0.135	1.181LeuCys: 1.181 ± 0.061	6.072LeuAsp: 6.072 ± 0.134	4.684LeuGlu: 4.684 ± 0.108	4.169LeuPhe: 4.169 ± 0.122	5.425LeuGly: 5.425 ± 0.127	1.094LeuHis: 1.094 ± 0.053	7.05LeuIle: 7.05 ± 0.166	7.581LeuLys: 7.581 ± 0.153	7.8LeuLeu: 7.8 ± 0.193	2.311LeuMet: 2.311 ± 0.08	5.587LeuAsn: 5.587 ± 0.119	3.064LeuPro: 3.064 ± 0.09	1.732LeuGln: 1.732 ± 0.062	2.961LeuArg: 2.961 ± 0.089	7.939LeuSer: 7.939 ± 0.163	5.254LeuThr: 5.254 ± 0.125	5.363LeuVal: 5.363 ± 0.118	0.615LeuTrp: 0.615 ± 0.038	3.396LeuTyr: 3.396 ± 0.102	0.0LeuXaa: 0.0 ± 0.0
Met	1.741MetAla: 1.741 ± 0.068	0.255MetCys: 0.255 ± 0.026	1.753MetAsp: 1.753 ± 0.073	1.532MetGlu: 1.532 ± 0.06	0.994MetPhe: 0.994 ± 0.06	1.404MetGly: 1.404 ± 0.06	0.269MetHis: 0.269 ± 0.027	1.956MetIle: 1.956 ± 0.068	2.245MetLys: 2.245 ± 0.066	1.767MetLeu: 1.767 ± 0.061	0.768MetMet: 0.768 ± 0.051	1.691MetAsn: 1.691 ± 0.073	0.903MetPro: 0.903 ± 0.049	0.568MetGln: 0.568 ± 0.034	0.878MetArg: 0.878 ± 0.043	1.892MetSer: 1.892 ± 0.068	1.612MetThr: 1.612 ± 0.054	1.534MetVal: 1.534 ± 0.065	0.166MetTrp: 0.166 ± 0.02	0.798MetTyr: 0.798 ± 0.044	0.0MetXaa: 0.0 ± 0.0
Asn	3.123AsnAla: 3.123 ± 0.077	0.599AsnCys: 0.599 ± 0.033	4.021AsnAsp: 4.021 ± 0.108	3.704AsnGlu: 3.704 ± 0.096	2.475AsnPhe: 2.475 ± 0.078	4.142AsnGly: 4.142 ± 0.113	0.898AsnHis: 0.898 ± 0.042	4.497AsnIle: 4.497 ± 0.117	5.366AsnLys: 5.366 ± 0.139	4.969AsnLeu: 4.969 ± 0.116	1.322AsnMet: 1.322 ± 0.057	3.841AsnAsn: 3.841 ± 0.112	2.548AsnPro: 2.548 ± 0.075	1.814AsnGln: 1.814 ± 0.071	1.839AsnArg: 1.839 ± 0.057	4.408AsnSer: 4.408 ± 0.106	2.913AsnThr: 2.913 ± 0.089	3.451AsnVal: 3.451 ± 0.083	0.483AsnTrp: 0.483 ± 0.033	2.97AsnTyr: 2.97 ± 0.095	0.0AsnXaa: 0.0 ± 0.0
Pro	1.477ProAla: 1.477 ± 0.067	0.294ProCys: 0.294 ± 0.023	2.038ProAsp: 2.038 ± 0.072	2.191ProGlu: 2.191 ± 0.082	1.796ProPhe: 1.796 ± 0.062	1.687ProGly: 1.687 ± 0.071	0.422ProHis: 0.422 ± 0.033	2.364ProIle: 2.364 ± 0.081	2.282ProLys: 2.282 ± 0.071	2.842ProLeu: 2.842 ± 0.081	0.709ProMet: 0.709 ± 0.041	1.805ProAsn: 1.805 ± 0.065	0.451ProPro: 0.451 ± 0.033	0.709ProGln: 0.709 ± 0.044	0.841ProArg: 0.841 ± 0.045	2.883ProSer: 2.883 ± 0.094	2.013ProThr: 2.013 ± 0.067	2.188ProVal: 2.188 ± 0.107	0.248ProTrp: 0.248 ± 0.023	1.632ProTyr: 1.632 ± 0.07	0.002ProXaa: 0.002 ± 0.002
Gln	1.721GlnAla: 1.721 ± 0.066	0.23GlnCys: 0.23 ± 0.024	1.404GlnAsp: 1.404 ± 0.059	1.614GlnGlu: 1.614 ± 0.061	1.106GlnPhe: 1.106 ± 0.046	1.577GlnGly: 1.577 ± 0.063	0.349GlnHis: 0.349 ± 0.029	2.143GlnIle: 2.143 ± 0.078	2.32GlnLys: 2.32 ± 0.09	2.131GlnLeu: 2.131 ± 0.068	0.745GlnMet: 0.745 ± 0.039	1.596GlnAsn: 1.596 ± 0.061	0.688GlnPro: 0.688 ± 0.044	0.72GlnGln: 0.72 ± 0.047	1.108GlnArg: 1.108 ± 0.053	1.726GlnSer: 1.726 ± 0.06	1.459GlnThr: 1.459 ± 0.06	1.541GlnVal: 1.541 ± 0.069	0.189GlnTrp: 0.189 ± 0.023	1.163GlnTyr: 1.163 ± 0.053	0.002GlnXaa: 0.002 ± 0.002
Arg	1.805ArgAla: 1.805 ± 0.073	0.433ArgCys: 0.433 ± 0.032	2.054ArgAsp: 2.054 ± 0.075	2.152ArgGlu: 2.152 ± 0.076	1.673ArgPhe: 1.673 ± 0.066	1.849ArgGly: 1.849 ± 0.069	0.545ArgHis: 0.545 ± 0.034	2.861ArgIle: 2.861 ± 0.088	3.038ArgLys: 3.038 ± 0.088	2.952ArgLeu: 2.952 ± 0.099	0.964ArgMet: 0.964 ± 0.046	2.156ArgAsn: 2.156 ± 0.068	1.039ArgPro: 1.039 ± 0.056	1.003ArgGln: 1.003 ± 0.045	1.511ArgArg: 1.511 ± 0.07	1.883ArgSer: 1.883 ± 0.067	1.753ArgThr: 1.753 ± 0.063	2.054ArgVal: 2.054 ± 0.073	0.267ArgTrp: 0.267 ± 0.025	1.486ArgTyr: 1.486 ± 0.057	0.002ArgXaa: 0.002 ± 0.002
Ser	3.367SerAla: 3.367 ± 0.104	0.935SerCys: 0.935 ± 0.052	4.367SerAsp: 4.367 ± 0.101	3.538SerGlu: 3.538 ± 0.1	4.598SerPhe: 4.598 ± 0.111	4.579SerGly: 4.579 ± 0.116	1.017SerHis: 1.017 ± 0.047	6.54SerIle: 6.54 ± 0.148	6.41SerLys: 6.41 ± 0.13	7.419SerLeu: 7.419 ± 0.153	1.712SerMet: 1.712 ± 0.057	4.698SerAsn: 4.698 ± 0.119	1.865SerPro: 1.865 ± 0.068	1.983SerGln: 1.983 ± 0.061	2.106SerArg: 2.106 ± 0.077	6.143SerSer: 6.143 ± 0.165	4.119SerThr: 4.119 ± 0.115	4.137SerVal: 4.137 ± 0.104	0.615SerTrp: 0.615 ± 0.039	3.982SerTyr: 3.982 ± 0.118	0.0SerXaa: 0.0 ± 0.0
Thr	3.223ThrAla: 3.223 ± 0.11	0.558ThrCys: 0.558 ± 0.036	3.506ThrAsp: 3.506 ± 0.102	2.79ThrGlu: 2.79 ± 0.086	2.836ThrPhe: 2.836 ± 0.095	3.663ThrGly: 3.663 ± 0.107	0.811ThrHis: 0.811 ± 0.042	4.919ThrIle: 4.919 ± 0.132	4.176ThrLys: 4.176 ± 0.115	5.455ThrLeu: 5.455 ± 0.108	1.194ThrMet: 1.194 ± 0.055	3.212ThrAsn: 3.212 ± 0.103	2.245ThrPro: 2.245 ± 0.083	1.352ThrGln: 1.352 ± 0.061	1.591ThrArg: 1.591 ± 0.055	4.194ThrSer: 4.194 ± 0.121	3.355ThrThr: 3.355 ± 0.138	3.374ThrVal: 3.374 ± 0.115	0.495ThrTrp: 0.495 ± 0.04	2.758ThrTyr: 2.758 ± 0.094	0.0ThrXaa: 0.0 ± 0.0
Val	3.492ValAla: 3.492 ± 0.108	0.818ValCys: 0.818 ± 0.048	4.256ValAsp: 4.256 ± 0.103	4.012ValGlu: 4.012 ± 0.115	3.166ValPhe: 3.166 ± 0.086	3.948ValGly: 3.948 ± 0.104	0.805ValHis: 0.805 ± 0.044	4.837ValIle: 4.837 ± 0.114	5.115ValLys: 5.115 ± 0.114	5.856ValLeu: 5.856 ± 0.11	1.477ValMet: 1.477 ± 0.06	3.244ValAsn: 3.244 ± 0.085	1.885ValPro: 1.885 ± 0.07	1.372ValGln: 1.372 ± 0.059	2.029ValArg: 2.029 ± 0.072	4.734ValSer: 4.734 ± 0.123	3.741ValThr: 3.741 ± 0.119	4.443ValVal: 4.443 ± 0.109	0.449ValTrp: 0.449 ± 0.033	2.612ValTyr: 2.612 ± 0.075	0.002ValXaa: 0.002 ± 0.002
Trp	0.488TrpAla: 0.488 ± 0.033	0.096TrpCys: 0.096 ± 0.016	0.504TrpAsp: 0.504 ± 0.035	0.362TrpGlu: 0.362 ± 0.032	0.415TrpPhe: 0.415 ± 0.029	0.531TrpGly: 0.531 ± 0.036	0.148TrpHis: 0.148 ± 0.022	0.691TrpIle: 0.691 ± 0.04	0.584TrpLys: 0.584 ± 0.033	0.702TrpLeu: 0.702 ± 0.04	0.246TrpMet: 0.246 ± 0.022	0.634TrpAsn: 0.634 ± 0.049	0.198TrpPro: 0.198 ± 0.024	0.173TrpGln: 0.173 ± 0.021	0.237TrpArg: 0.237 ± 0.022	0.554TrpSer: 0.554 ± 0.039	0.435TrpThr: 0.435 ± 0.035	0.444TrpVal: 0.444 ± 0.031	0.13TrpTrp: 0.13 ± 0.018	0.41TrpTyr: 0.41 ± 0.048	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.357TyrAla: 2.357 ± 0.081	0.638TyrCys: 0.638 ± 0.045	3.232TyrAsp: 3.232 ± 0.108	2.774TyrGlu: 2.774 ± 0.083	2.444TyrPhe: 2.444 ± 0.073	2.913TyrGly: 2.913 ± 0.085	0.704TyrHis: 0.704 ± 0.038	3.068TyrIle: 3.068 ± 0.089	3.85TyrLys: 3.85 ± 0.103	4.411TyrLeu: 4.411 ± 0.124	0.914TyrMet: 0.914 ± 0.048	2.393TyrAsn: 2.393 ± 0.09	1.648TyrPro: 1.648 ± 0.062	1.365TyrGln: 1.365 ± 0.056	1.666TyrArg: 1.666 ± 0.064	3.695TyrSer: 3.695 ± 0.094	2.35TyrThr: 2.35 ± 0.086	2.833TyrVal: 2.833 ± 0.081	0.449TyrTrp: 0.449 ± 0.047	2.227TyrTyr: 2.227 ± 0.081	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.002XaaAsp: 0.002 ± 0.002	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.003	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.002	0.005XaaSer: 0.005 ± 0.003	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.021XaaXaa: 0.021 ± 0.01

Statistics based on 1211 proteins (438710 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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