Synechococcus phage S-CBP1
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A096VKD1|A0A096VKD1_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBP1 OX=1273711 GN=S-CBP1_0008 PE=4 SV=1
MM1 pKa = 6.97 TTTIPIVLTGQSLVDD16 pKa = 3.6 YY17 pKa = 10.39 VDD19 pKa = 3.2 EE20 pKa = 4.57 RR21 pKa = 11.84 KK22 pKa = 10.24 ALIEE26 pKa = 4.27 RR27 pKa = 11.84 GEE29 pKa = 4.07 LTRR32 pKa = 11.84 TDD34 pKa = 4.64 LIKK37 pKa = 10.96 DD38 pKa = 3.26 AGYY41 pKa = 11.23 VSDD44 pKa = 4.09 NGKK47 pKa = 9.94 ARR49 pKa = 11.84 YY50 pKa = 9.32 VDD52 pKa = 5.86 FYY54 pKa = 11.4 TEE56 pKa = 4.04 LLNARR61 pKa = 11.84 GITPVTNRR69 pKa = 11.84 DD70 pKa = 3.35 IEE72 pKa = 4.55 DD73 pKa = 3.71 NEE75 pKa = 3.87 YY76 pKa = 11.16 DD77 pKa = 4.81 ALNEE81 pKa = 4.16 DD82 pKa = 3.79 EE83 pKa = 4.93 KK84 pKa = 11.67 ALYY87 pKa = 10.14 DD88 pKa = 3.92 YY89 pKa = 11.32 LDD91 pKa = 3.84 EE92 pKa = 5.04 QSFTEE97 pKa = 4.15 KK98 pKa = 9.38 WDD100 pKa = 3.39 HH101 pKa = 6.38 EE102 pKa = 4.36 EE103 pKa = 4.29 FIEE106 pKa = 5.32 FLVEE110 pKa = 4.17 LKK112 pKa = 11.03 DD113 pKa = 3.69 NGIEE117 pKa = 4.27 TVSSFEE123 pKa = 4.33 DD124 pKa = 3.03 AFYY127 pKa = 10.85 SYY129 pKa = 11.43 NDD131 pKa = 3.58 EE132 pKa = 4.26 YY133 pKa = 10.38 WAEE136 pKa = 3.73 RR137 pKa = 11.84 HH138 pKa = 5.44 FAEE141 pKa = 4.95 EE142 pKa = 4.16 YY143 pKa = 8.85 VNEE146 pKa = 4.21 VEE148 pKa = 5.33 SIQDD152 pKa = 3.62 SIVYY156 pKa = 9.31 HH157 pKa = 6.66 AIDD160 pKa = 3.48 WQAVWDD166 pKa = 3.85 HH167 pKa = 4.94 QLRR170 pKa = 11.84 YY171 pKa = 10.14 DD172 pKa = 3.96 FCTIEE177 pKa = 3.91 HH178 pKa = 6.53 GGWTFFFRR186 pKa = 11.84 NYY188 pKa = 10.46
Molecular weight: 22.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.975
IPC2_protein 4.126
IPC_protein 4.088
Toseland 3.91
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.948
Rodwell 3.923
Grimsley 3.821
Solomon 4.05
Lehninger 3.999
Nozaki 4.164
DTASelect 4.342
Thurlkill 3.935
EMBOSS 3.961
Sillero 4.202
Patrickios 2.587
IPC_peptide 4.05
IPC2_peptide 4.19
IPC2.peptide.svr19 4.095
Protein with the highest isoelectric point:
>tr|A0A096VKE7|A0A096VKE7_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBP1 OX=1273711 GN=S-CBP1_0023 PE=4 SV=1
MM1 pKa = 6.26 TRR3 pKa = 11.84 ARR5 pKa = 11.84 IEE7 pKa = 3.97 YY8 pKa = 10.12 ARR10 pKa = 11.84 GRR12 pKa = 11.84 GGGGRR17 pKa = 11.84 GGGGRR22 pKa = 11.84 GGGSRR27 pKa = 11.84 GRR29 pKa = 11.84 GGGGGGRR36 pKa = 11.84 SSGGGGGGRR45 pKa = 11.84 SSGGGGGGGRR55 pKa = 11.84 AAASSAGRR63 pKa = 11.84 SAASTGSFTSANAQALRR80 pKa = 11.84 QAGVSRR86 pKa = 11.84 SRR88 pKa = 11.84 IQNLRR93 pKa = 11.84 SQSQNINNRR102 pKa = 11.84 NSAAADD108 pKa = 3.04 ARR110 pKa = 11.84 AAMDD114 pKa = 4.22 PSVAGAMEE122 pKa = 4.12 AQANRR127 pKa = 11.84 GLNIGQQFYY136 pKa = 10.96 DD137 pKa = 3.46 SPEE140 pKa = 3.86 FQQALGQSGLSPQQIAQYY158 pKa = 11.33 AMSQGYY164 pKa = 8.9 GLGNQFMDD172 pKa = 3.64 QFGTTTTNRR181 pKa = 11.84 SISGATKK188 pKa = 9.77 YY189 pKa = 10.41 RR190 pKa = 11.84 QALKK194 pKa = 10.19 IAAAQGRR201 pKa = 11.84 GTNLIGGRR209 pKa = 11.84 EE210 pKa = 4.05 AKK212 pKa = 10.41 QIAKK216 pKa = 9.3 QFKK219 pKa = 10.29 NKK221 pKa = 9.12 TDD223 pKa = 3.81 LNFARR228 pKa = 11.84 SLDD231 pKa = 3.88 KK232 pKa = 11.18 INANKK237 pKa = 9.43 PNMMPIGLTGGVLNRR252 pKa = 11.84 LSRR255 pKa = 11.84 NKK257 pKa = 10.43 DD258 pKa = 2.99 GSLSRR263 pKa = 11.84 MGMMPSSDD271 pKa = 3.62 GFLKK275 pKa = 10.52 ILANMNDD282 pKa = 3.13 RR283 pKa = 11.84 WTAGDD288 pKa = 3.72 ARR290 pKa = 11.84 NGNPSKK296 pKa = 10.75 RR297 pKa = 11.84 IPGTGKK303 pKa = 9.69 FRR305 pKa = 11.84 RR306 pKa = 11.84 TDD308 pKa = 3.48 TIYY311 pKa = 11.32 GLMSDD316 pKa = 3.76 GSFRR320 pKa = 11.84 QTPGVGKK327 pKa = 10.11 GWADD331 pKa = 3.26 TRR333 pKa = 11.84 FGQPEE338 pKa = 4.15 TTQYY342 pKa = 10.17 EE343 pKa = 4.29 PWYY346 pKa = 9.04 TPPEE350 pKa = 4.36 DD351 pKa = 3.29 QGVASVEE358 pKa = 4.4 DD359 pKa = 4.07 YY360 pKa = 11.65 GEE362 pKa = 4.43 APLPPEE368 pKa = 4.4 EE369 pKa = 5.33 PMTTDD374 pKa = 3.41 SNQSGAGADD383 pKa = 3.53 LASFATGWKK392 pKa = 8.36 GARR395 pKa = 11.84 GARR398 pKa = 11.84 ARR400 pKa = 11.84 AGRR403 pKa = 11.84 KK404 pKa = 8.64 AQGTGSMRR412 pKa = 11.84 IAPTYY417 pKa = 9.38 GAGVGSNFGG426 pKa = 3.39
Molecular weight: 43.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.313
IPC2_protein 9.897
IPC_protein 10.965
Toseland 11.008
ProMoST 11.052
Dawson 11.067
Bjellqvist 10.891
Wikipedia 11.389
Rodwell 11.111
Grimsley 11.125
Solomon 11.33
Lehninger 11.272
Nozaki 10.994
DTASelect 10.891
Thurlkill 11.008
EMBOSS 11.447
Sillero 11.023
Patrickios 10.818
IPC_peptide 11.345
IPC2_peptide 10.043
IPC2.peptide.svr19 8.623
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
50
0
50
13376
32
1402
267.5
29.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.824 ± 0.621
0.733 ± 0.159
6.347 ± 0.3
6.011 ± 0.422
3.514 ± 0.186
7.401 ± 0.447
1.391 ± 0.185
5.084 ± 0.208
5.233 ± 0.457
8.074 ± 0.348
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.385 ± 0.197
4.987 ± 0.313
4.224 ± 0.229
4.575 ± 0.296
5.51 ± 0.351
6.788 ± 0.463
6.699 ± 0.596
6.28 ± 0.344
1.368 ± 0.147
3.574 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here