Amino acid dipepetide frequency for Bacillus circulans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.269AlaAla: 5.269 ± 0.072
0.587AlaCys: 0.587 ± 0.02
3.188AlaAsp: 3.188 ± 0.053
4.492AlaGlu: 4.492 ± 0.065
3.104AlaPhe: 3.104 ± 0.055
4.717AlaGly: 4.717 ± 0.074
1.109AlaHis: 1.109 ± 0.029
6.145AlaIle: 6.145 ± 0.072
4.773AlaLys: 4.773 ± 0.067
6.637AlaLeu: 6.637 ± 0.087
1.792AlaMet: 1.792 ± 0.037
2.905AlaAsn: 2.905 ± 0.047
1.759AlaPro: 1.759 ± 0.04
2.006AlaGln: 2.006 ± 0.042
2.277AlaArg: 2.277 ± 0.045
3.959AlaSer: 3.959 ± 0.056
3.198AlaThr: 3.198 ± 0.056
4.79AlaVal: 4.79 ± 0.059
0.599AlaTrp: 0.599 ± 0.021
2.311AlaTyr: 2.311 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.42CysAla: 0.42 ± 0.016
0.113CysCys: 0.113 ± 0.01
0.339CysAsp: 0.339 ± 0.016
0.476CysGlu: 0.476 ± 0.019
0.34CysPhe: 0.34 ± 0.015
0.61CysGly: 0.61 ± 0.021
0.183CysHis: 0.183 ± 0.011
0.604CysIle: 0.604 ± 0.02
0.418CysLys: 0.418 ± 0.021
0.75CysLeu: 0.75 ± 0.024
0.2CysMet: 0.2 ± 0.012
0.302CysAsn: 0.302 ± 0.014
0.313CysPro: 0.313 ± 0.014
0.232CysGln: 0.232 ± 0.013
0.288CysArg: 0.288 ± 0.016
0.498CysSer: 0.498 ± 0.018
0.393CysThr: 0.393 ± 0.016
0.407CysVal: 0.407 ± 0.018
0.069CysTrp: 0.069 ± 0.007
0.285CysTyr: 0.285 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
2.822AspAla: 2.822 ± 0.047
0.375AspCys: 0.375 ± 0.015
2.213AspAsp: 2.213 ± 0.047
3.888AspGlu: 3.888 ± 0.059
2.536AspPhe: 2.536 ± 0.047
3.188AspGly: 3.188 ± 0.048
0.998AspHis: 0.998 ± 0.032
4.448AspIle: 4.448 ± 0.061
3.595AspLys: 3.595 ± 0.049
4.862AspLeu: 4.862 ± 0.063
1.221AspMet: 1.221 ± 0.03
2.119AspAsn: 2.119 ± 0.044
1.767AspPro: 1.767 ± 0.037
1.774AspGln: 1.774 ± 0.037
2.003AspArg: 2.003 ± 0.04
2.691AspSer: 2.691 ± 0.046
2.564AspThr: 2.564 ± 0.047
3.167AspVal: 3.167 ± 0.05
0.682AspTrp: 0.682 ± 0.024
2.376AspTyr: 2.376 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
5.075GluAla: 5.075 ± 0.064
0.376GluCys: 0.376 ± 0.017
3.793GluAsp: 3.793 ± 0.054
7.653GluGlu: 7.653 ± 0.1
2.518GluPhe: 2.518 ± 0.047
4.081GluGly: 4.081 ± 0.058
1.388GluHis: 1.388 ± 0.032
6.028GluIle: 6.028 ± 0.07
7.286GluLys: 7.286 ± 0.08
7.241GluLeu: 7.241 ± 0.075
2.331GluMet: 2.331 ± 0.047
4.166GluAsn: 4.166 ± 0.062
1.789GluPro: 1.789 ± 0.043
3.242GluGln: 3.242 ± 0.051
3.189GluArg: 3.189 ± 0.048
3.634GluSer: 3.634 ± 0.056
3.775GluThr: 3.775 ± 0.056
5.02GluVal: 5.02 ± 0.066
0.912GluTrp: 0.912 ± 0.027
2.506GluTyr: 2.506 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
3.061PheAla: 3.061 ± 0.049
0.388PheCys: 0.388 ± 0.017
2.213PheAsp: 2.213 ± 0.039
2.638PheGlu: 2.638 ± 0.049
2.504PhePhe: 2.504 ± 0.06
3.112PheGly: 3.112 ± 0.052
1.102PheHis: 1.102 ± 0.03
4.231PheIle: 4.231 ± 0.076
2.6PheLys: 2.6 ± 0.049
4.957PheLeu: 4.957 ± 0.078
1.186PheMet: 1.186 ± 0.028
1.963PheAsn: 1.963 ± 0.037
1.733PhePro: 1.733 ± 0.039
1.782PheGln: 1.782 ± 0.035
1.466PheArg: 1.466 ± 0.034
3.489PheSer: 3.489 ± 0.055
2.659PheThr: 2.659 ± 0.045
2.923PheVal: 2.923 ± 0.049
0.49PheTrp: 0.49 ± 0.018
1.903PheTyr: 1.903 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
4.272GlyAla: 4.272 ± 0.073
0.613GlyCys: 0.613 ± 0.028
2.844GlyAsp: 2.844 ± 0.05
4.397GlyGlu: 4.397 ± 0.057
3.314GlyPhe: 3.314 ± 0.059
4.31GlyGly: 4.31 ± 0.07
1.164GlyHis: 1.164 ± 0.031
6.249GlyIle: 6.249 ± 0.084
5.23GlyLys: 5.23 ± 0.059
6.143GlyLeu: 6.143 ± 0.078
1.996GlyMet: 1.996 ± 0.042
2.809GlyAsn: 2.809 ± 0.047
1.566GlyPro: 1.566 ± 0.036
1.933GlyGln: 1.933 ± 0.039
2.213GlyArg: 2.213 ± 0.038
3.734GlySer: 3.734 ± 0.046
3.651GlyThr: 3.651 ± 0.048
4.559GlyVal: 4.559 ± 0.065
0.768GlyTrp: 0.768 ± 0.024
2.868GlyTyr: 2.868 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
1.17HisAla: 1.17 ± 0.032
0.203HisCys: 0.203 ± 0.012
0.895HisAsp: 0.895 ± 0.028
1.178HisGlu: 1.178 ± 0.027
1.085HisPhe: 1.085 ± 0.032
1.247HisGly: 1.247 ± 0.032
0.62HisHis: 0.62 ± 0.025
1.579HisIle: 1.579 ± 0.035
1.115HisLys: 1.115 ± 0.026
2.166HisLeu: 2.166 ± 0.042
0.455HisMet: 0.455 ± 0.018
0.792HisAsn: 0.792 ± 0.024
1.005HisPro: 1.005 ± 0.027
0.799HisGln: 0.799 ± 0.025
0.772HisArg: 0.772 ± 0.024
1.256HisSer: 1.256 ± 0.034
1.102HisThr: 1.102 ± 0.032
1.169HisVal: 1.169 ± 0.027
0.239HisTrp: 0.239 ± 0.012
0.939HisTyr: 0.939 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
6.142IleAla: 6.142 ± 0.076
0.741IleCys: 0.741 ± 0.026
4.492IleAsp: 4.492 ± 0.055
5.872IleGlu: 5.872 ± 0.076
3.719IlePhe: 3.719 ± 0.061
6.275IleGly: 6.275 ± 0.084
1.834IleHis: 1.834 ± 0.04
7.152IleIle: 7.152 ± 0.099
5.607IleLys: 5.607 ± 0.072
8.116IleLeu: 8.116 ± 0.086
2.0IleMet: 2.0 ± 0.044
3.925IleAsn: 3.925 ± 0.055
3.668IlePro: 3.668 ± 0.043
3.08IleGln: 3.08 ± 0.051
3.125IleArg: 3.125 ± 0.053
5.759IleSer: 5.759 ± 0.08
4.867IleThr: 4.867 ± 0.066
5.747IleVal: 5.747 ± 0.077
0.719IleTrp: 0.719 ± 0.022
2.893IleTyr: 2.893 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
4.638LysAla: 4.638 ± 0.062
0.346LysCys: 0.346 ± 0.016
4.201LysAsp: 4.201 ± 0.071
8.27LysGlu: 8.27 ± 0.098
2.089LysPhe: 2.089 ± 0.037
4.733LysGly: 4.733 ± 0.057
1.317LysHis: 1.317 ± 0.028
5.477LysIle: 5.477 ± 0.072
6.897LysLys: 6.897 ± 0.079
6.272LysLeu: 6.272 ± 0.078
2.382LysMet: 2.382 ± 0.042
3.962LysAsn: 3.962 ± 0.061
2.143LysPro: 2.143 ± 0.037
3.273LysGln: 3.273 ± 0.056
3.242LysArg: 3.242 ± 0.042
3.702LysSer: 3.702 ± 0.054
3.801LysThr: 3.801 ± 0.058
4.914LysVal: 4.914 ± 0.067
0.948LysTrp: 0.948 ± 0.025
2.519LysTyr: 2.519 ± 0.043
0.0LysXaa: 0.0 ± 0.0
Leu
7.178LeuAla: 7.178 ± 0.078
0.664LeuCys: 0.664 ± 0.024
4.772LeuAsp: 4.772 ± 0.063
7.076LeuGlu: 7.076 ± 0.067
5.074LeuPhe: 5.074 ± 0.078
6.053LeuGly: 6.053 ± 0.082
1.972LeuHis: 1.972 ± 0.039
7.789LeuIle: 7.789 ± 0.094
6.891LeuLys: 6.891 ± 0.069
10.687LeuLeu: 10.687 ± 0.109
2.505LeuMet: 2.505 ± 0.042
4.577LeuAsn: 4.577 ± 0.057
3.73LeuPro: 3.73 ± 0.058
3.731LeuGln: 3.731 ± 0.06
3.437LeuArg: 3.437 ± 0.063
6.969LeuSer: 6.969 ± 0.082
5.637LeuThr: 5.637 ± 0.065
6.113LeuVal: 6.113 ± 0.075
0.843LeuTrp: 0.843 ± 0.024
3.263LeuTyr: 3.263 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
1.809MetAla: 1.809 ± 0.038
0.148MetCys: 0.148 ± 0.011
1.515MetAsp: 1.515 ± 0.033
2.341MetGlu: 2.341 ± 0.043
0.998MetPhe: 0.998 ± 0.025
1.624MetGly: 1.624 ± 0.031
0.416MetHis: 0.416 ± 0.017
2.231MetIle: 2.231 ± 0.043
2.506MetLys: 2.506 ± 0.044
2.461MetLeu: 2.461 ± 0.04
0.82MetMet: 0.82 ± 0.024
1.591MetAsn: 1.591 ± 0.035
0.964MetPro: 0.964 ± 0.026
0.931MetGln: 0.931 ± 0.028
0.998MetArg: 0.998 ± 0.026
1.547MetSer: 1.547 ± 0.033
1.465MetThr: 1.465 ± 0.035
1.697MetVal: 1.697 ± 0.038
0.18MetTrp: 0.18 ± 0.012
0.754MetTyr: 0.754 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
2.741AsnAla: 2.741 ± 0.049
0.319AsnCys: 0.319 ± 0.018
2.283AsnAsp: 2.283 ± 0.041
3.6AsnGlu: 3.6 ± 0.053
1.82AsnPhe: 1.82 ± 0.04
3.553AsnGly: 3.553 ± 0.049
1.137AsnHis: 1.137 ± 0.032
4.017AsnIle: 4.017 ± 0.052
3.757AsnLys: 3.757 ± 0.059
4.128AsnLeu: 4.128 ± 0.052
1.282AsnMet: 1.282 ± 0.034
2.512AsnAsn: 2.512 ± 0.044
2.148AsnPro: 2.148 ± 0.04
2.086AsnGln: 2.086 ± 0.039
2.162AsnArg: 2.162 ± 0.037
2.611AsnSer: 2.611 ± 0.046
2.366AsnThr: 2.366 ± 0.042
2.922AsnVal: 2.922 ± 0.047
0.599AsnTrp: 0.599 ± 0.023
1.896AsnTyr: 1.896 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
2.091ProAla: 2.091 ± 0.038
0.215ProCys: 0.215 ± 0.012
1.77ProAsp: 1.77 ± 0.043
2.639ProGlu: 2.639 ± 0.044
2.039ProPhe: 2.039 ± 0.038
1.939ProGly: 1.939 ± 0.048
0.713ProHis: 0.713 ± 0.024
3.113ProIle: 3.113 ± 0.046
2.21ProLys: 2.21 ± 0.038
3.411ProLeu: 3.411 ± 0.052
0.771ProMet: 0.771 ± 0.025
1.739ProAsn: 1.739 ± 0.036
0.853ProPro: 0.853 ± 0.026
1.023ProGln: 1.023 ± 0.03
0.94ProArg: 0.94 ± 0.025
2.224ProSer: 2.224 ± 0.044
1.936ProThr: 1.936 ± 0.033
2.515ProVal: 2.515 ± 0.044
0.335ProTrp: 0.335 ± 0.016
1.438ProTyr: 1.438 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
2.498GlnAla: 2.498 ± 0.05
0.208GlnCys: 0.208 ± 0.012
1.559GlnAsp: 1.559 ± 0.035
2.932GlnGlu: 2.932 ± 0.054
1.582GlnPhe: 1.582 ± 0.033
1.971GlnGly: 1.971 ± 0.031
0.708GlnHis: 0.708 ± 0.022
2.832GlnIle: 2.832 ± 0.05
2.969GlnLys: 2.969 ± 0.049
4.093GlnLeu: 4.093 ± 0.055
1.076GlnMet: 1.076 ± 0.026
1.719GlnAsn: 1.719 ± 0.037
1.101GlnPro: 1.101 ± 0.034
1.731GlnGln: 1.731 ± 0.04
1.405GlnArg: 1.405 ± 0.029
2.136GlnSer: 2.136 ± 0.041
1.979GlnThr: 1.979 ± 0.039
2.171GlnVal: 2.171 ± 0.037
0.429GlnTrp: 0.429 ± 0.017
1.477GlnTyr: 1.477 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
2.175ArgAla: 2.175 ± 0.044
0.222ArgCys: 0.222 ± 0.014
1.871ArgAsp: 1.871 ± 0.033
3.043ArgGlu: 3.043 ± 0.05
1.734ArgPhe: 1.734 ± 0.039
2.115ArgGly: 2.115 ± 0.045
0.675ArgHis: 0.675 ± 0.023
3.136ArgIle: 3.136 ± 0.045
3.435ArgLys: 3.435 ± 0.051
3.692ArgLeu: 3.692 ± 0.054
1.203ArgMet: 1.203 ± 0.03
1.999ArgAsn: 1.999 ± 0.037
1.085ArgPro: 1.085 ± 0.033
1.341ArgGln: 1.341 ± 0.037
1.637ArgArg: 1.637 ± 0.036
1.963ArgSer: 1.963 ± 0.037
1.873ArgThr: 1.873 ± 0.039
2.28ArgVal: 2.28 ± 0.043
0.385ArgTrp: 0.385 ± 0.016
1.528ArgTyr: 1.528 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
3.491SerAla: 3.491 ± 0.049
0.401SerCys: 0.401 ± 0.016
2.785SerAsp: 2.785 ± 0.046
3.762SerGlu: 3.762 ± 0.057
3.597SerPhe: 3.597 ± 0.055
3.969SerGly: 3.969 ± 0.057
1.172SerHis: 1.172 ± 0.029
6.047SerIle: 6.047 ± 0.078
4.238SerLys: 4.238 ± 0.06
6.6SerLeu: 6.6 ± 0.074
1.62SerMet: 1.62 ± 0.035
2.836SerAsn: 2.836 ± 0.046
2.092SerPro: 2.092 ± 0.036
1.896SerGln: 1.896 ± 0.037
2.167SerArg: 2.167 ± 0.039
4.199SerSer: 4.199 ± 0.063
3.286SerThr: 3.286 ± 0.053
3.919SerVal: 3.919 ± 0.051
0.629SerTrp: 0.629 ± 0.023
2.442SerTyr: 2.442 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
3.711ThrAla: 3.711 ± 0.056
0.344ThrCys: 0.344 ± 0.015
2.624ThrAsp: 2.624 ± 0.045
3.584ThrGlu: 3.584 ± 0.049
2.695ThrPhe: 2.695 ± 0.052
3.899ThrGly: 3.899 ± 0.056
0.939ThrHis: 0.939 ± 0.026
5.176ThrIle: 5.176 ± 0.071
3.665ThrLys: 3.665 ± 0.047
5.189ThrLeu: 5.189 ± 0.062
1.198ThrMet: 1.198 ± 0.032
2.704ThrAsn: 2.704 ± 0.043
2.119ThrPro: 2.119 ± 0.044
1.4ThrGln: 1.4 ± 0.034
1.724ThrArg: 1.724 ± 0.033
3.38ThrSer: 3.38 ± 0.053
2.9ThrThr: 2.9 ± 0.052
3.933ThrVal: 3.933 ± 0.06
0.513ThrTrp: 0.513 ± 0.019
1.957ThrTyr: 1.957 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
4.388ValAla: 4.388 ± 0.065
0.56ValCys: 0.56 ± 0.019
3.441ValAsp: 3.441 ± 0.049
4.791ValGlu: 4.791 ± 0.068
3.087ValPhe: 3.087 ± 0.053
4.119ValGly: 4.119 ± 0.063
1.185ValHis: 1.185 ± 0.028
5.656ValIle: 5.656 ± 0.071
4.652ValLys: 4.652 ± 0.05
6.462ValLeu: 6.462 ± 0.075
1.67ValMet: 1.67 ± 0.034
3.083ValAsn: 3.083 ± 0.049
2.38ValPro: 2.38 ± 0.04
2.178ValGln: 2.178 ± 0.04
2.305ValArg: 2.305 ± 0.039
4.401ValSer: 4.401 ± 0.051
3.752ValThr: 3.752 ± 0.051
4.347ValVal: 4.347 ± 0.067
0.611ValTrp: 0.611 ± 0.022
2.298ValTyr: 2.298 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
0.572TrpAla: 0.572 ± 0.023
0.072TrpCys: 0.072 ± 0.007
0.565TrpAsp: 0.565 ± 0.021
0.733TrpGlu: 0.733 ± 0.022
0.512TrpPhe: 0.512 ± 0.021
0.683TrpGly: 0.683 ± 0.024
0.225TrpHis: 0.225 ± 0.011
0.907TrpIle: 0.907 ± 0.027
0.867TrpLys: 0.867 ± 0.028
1.126TrpLeu: 1.126 ± 0.032
0.339TrpMet: 0.339 ± 0.016
0.608TrpAsn: 0.608 ± 0.021
0.222TrpPro: 0.222 ± 0.012
0.393TrpGln: 0.393 ± 0.018
0.424TrpArg: 0.424 ± 0.019
0.637TrpSer: 0.637 ± 0.022
0.538TrpThr: 0.538 ± 0.023
0.596TrpVal: 0.596 ± 0.023
0.138TrpTrp: 0.138 ± 0.009
0.374TrpTyr: 0.374 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.094TyrAla: 2.094 ± 0.037
0.345TyrCys: 0.345 ± 0.018
1.926TyrAsp: 1.926 ± 0.041
2.638TyrGlu: 2.638 ± 0.046
2.054TyrPhe: 2.054 ± 0.044
2.436TyrGly: 2.436 ± 0.045
0.928TyrHis: 0.928 ± 0.027
2.917TyrIle: 2.917 ± 0.048
2.414TyrLys: 2.414 ± 0.041
3.967TyrLeu: 3.967 ± 0.053
0.946TyrMet: 0.946 ± 0.025
1.626TyrAsn: 1.626 ± 0.041
1.477TyrPro: 1.477 ± 0.03
1.747TyrGln: 1.747 ± 0.036
1.618TyrArg: 1.618 ± 0.038
2.361TyrSer: 2.361 ± 0.043
1.912TyrThr: 1.912 ± 0.04
2.175TyrVal: 2.175 ± 0.047
0.443TyrTrp: 0.443 ± 0.017
1.685TyrTyr: 1.685 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4949 proteins (1429561 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski