Mosquito VEM Anellovirus SDRB B

Taxonomy: Viruses; Anelloviridae; unclassified Anelloviridae

Average proteome isoelectric point is 7.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6KID8|F6KID8_9VIRU Capsid protein OS=Mosquito VEM Anellovirus SDRB B OX=1034793 PE=3 SV=1
MM1 pKa = 7.46SEE3 pKa = 4.35SIEE6 pKa = 3.99LKK8 pKa = 10.62QILNYY13 pKa = 8.83KK14 pKa = 9.37QKK16 pKa = 9.66EE17 pKa = 3.96ALFRR21 pKa = 11.84RR22 pKa = 11.84QVRR25 pKa = 11.84LSHH28 pKa = 6.92QLFCNCGDD36 pKa = 4.25PLRR39 pKa = 11.84HH40 pKa = 5.04LQGWRR45 pKa = 11.84TTGDD49 pKa = 3.15EE50 pKa = 5.05GIQGTPGGEE59 pKa = 4.04QQTGGTPDD67 pKa = 3.66AEE69 pKa = 4.53DD70 pKa = 3.53MLDD73 pKa = 3.45ALMAAEE79 pKa = 4.42GG80 pKa = 3.59

Molecular weight: 8.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6KID9|F6KID9_9VIRU Uncharacterized protein OS=Mosquito VEM Anellovirus SDRB B OX=1034793 PE=4 SV=1
MM1 pKa = 7.68GLPLNVPLQEE11 pKa = 4.27LLNLISPQKK20 pKa = 9.93TPFSLKK26 pKa = 10.25DD27 pKa = 3.41KK28 pKa = 10.85RR29 pKa = 11.84SGAQSSIHH37 pKa = 5.26HH38 pKa = 6.08HH39 pKa = 4.71QEE41 pKa = 3.89KK42 pKa = 10.45EE43 pKa = 4.07NEE45 pKa = 4.34STDD48 pKa = 3.15TTRR51 pKa = 11.84AKK53 pKa = 10.31KK54 pKa = 9.88KK55 pKa = 9.81RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84TTALPXKK65 pKa = 8.99TRR67 pKa = 11.84RR68 pKa = 11.84EE69 pKa = 3.84KK70 pKa = 10.56PQQQRR75 pKa = 11.84KK76 pKa = 6.79RR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84DD80 pKa = 2.78IWSGSEE86 pKa = 3.99STEE89 pKa = 4.34SSSDD93 pKa = 3.05WSSDD97 pKa = 2.96

Molecular weight: 11.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

755

80

578

251.7

29.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.43 ± 0.71

1.192 ± 0.422

4.371 ± 0.67

6.225 ± 0.887

3.311 ± 0.86

6.49 ± 1.632

2.781 ± 0.102

5.166 ± 0.909

5.563 ± 1.439

7.285 ± 1.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.722 ± 0.528

3.709 ± 0.457

6.093 ± 0.648

5.033 ± 1.797

10.066 ± 0.993

6.887 ± 2.285

6.887 ± 0.412

3.841 ± 1.375

3.444 ± 0.894

4.371 ± 1.944
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski