Arthrobacter phage Wilde
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U4KBW5|A0A0U4KBW5_9CAUD Portal protein OS=Arthrobacter phage Wilde OX=1772323 GN=17 PE=4 SV=1
MM1 pKa = 7.95 ALRR4 pKa = 11.84 IIYY7 pKa = 8.23 KK8 pKa = 7.54 TEE10 pKa = 4.09 CDD12 pKa = 3.54 CGGEE16 pKa = 4.37 MVSVTHH22 pKa = 7.61 DD23 pKa = 3.53 LTQSGEE29 pKa = 4.2 DD30 pKa = 3.43 GTVTIDD36 pKa = 4.45 LDD38 pKa = 3.66 MLGDD42 pKa = 3.53 MTLICNKK49 pKa = 9.94 CDD51 pKa = 3.67 DD52 pKa = 4.02 QAFLPNVGDD61 pKa = 4.04 TIIDD65 pKa = 3.8 GDD67 pKa = 4.18 DD68 pKa = 3.08 LL69 pKa = 6.0
Molecular weight: 7.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.766
IPC2_protein 3.567
IPC_protein 3.541
Toseland 3.325
ProMoST 3.757
Dawson 3.567
Bjellqvist 3.732
Wikipedia 3.567
Rodwell 3.376
Grimsley 3.236
Solomon 3.541
Lehninger 3.49
Nozaki 3.706
DTASelect 3.973
Thurlkill 3.414
EMBOSS 3.567
Sillero 3.681
Patrickios 0.693
IPC_peptide 3.528
IPC2_peptide 3.643
IPC2.peptide.svr19 3.669
Protein with the highest isoelectric point:
>tr|A0A0U4KSM3|A0A0U4KSM3_9CAUD Uncharacterized protein OS=Arthrobacter phage Wilde OX=1772323 GN=3 PE=4 SV=1
MM1 pKa = 7.48 LTRR4 pKa = 11.84 KK5 pKa = 8.18 EE6 pKa = 3.83 LRR8 pKa = 11.84 EE9 pKa = 3.88 RR10 pKa = 11.84 RR11 pKa = 11.84 HH12 pKa = 5.78 RR13 pKa = 11.84 SYY15 pKa = 11.46 LVLNALYY22 pKa = 10.51 RR23 pKa = 11.84 EE24 pKa = 4.4 TPWYY28 pKa = 9.82 LLHH31 pKa = 6.32 EE32 pKa = 4.36 RR33 pKa = 11.84 SMIRR37 pKa = 11.84 EE38 pKa = 4.44 AMRR41 pKa = 11.84 SAGPVSAA48 pKa = 5.36
Molecular weight: 5.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.303
IPC2_protein 9.94
IPC_protein 11.038
Toseland 10.789
ProMoST 10.979
Dawson 10.906
Bjellqvist 10.804
Wikipedia 11.286
Rodwell 10.76
Grimsley 10.994
Solomon 11.184
Lehninger 11.111
Nozaki 10.76
DTASelect 10.804
Thurlkill 10.818
EMBOSS 11.242
Sillero 10.862
Patrickios 10.54
IPC_peptide 11.184
IPC2_peptide 10.087
IPC2.peptide.svr19 8.424
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
109
0
109
21287
31
2075
195.3
21.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.344 ± 0.416
0.864 ± 0.129
6.704 ± 0.192
6.929 ± 0.437
3.302 ± 0.201
8.202 ± 0.303
2.119 ± 0.206
5.219 ± 0.239
4.707 ± 0.258
7.361 ± 0.242
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.645 ± 0.108
3.537 ± 0.274
5.074 ± 0.322
3.378 ± 0.17
5.637 ± 0.287
5.788 ± 0.212
6.737 ± 0.286
6.647 ± 0.226
2.072 ± 0.141
2.734 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here