Amino acid dipepetide frequency for Mycobacterium phage Shandong1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.063AlaAla: 21.063 ± 1.507
1.0AlaCys: 1.0 ± 0.246
8.67AlaAsp: 8.67 ± 0.642
9.559AlaGlu: 9.559 ± 1.03
3.112AlaPhe: 3.112 ± 0.57
9.336AlaGly: 9.336 ± 0.999
2.668AlaHis: 2.668 ± 0.475
5.168AlaIle: 5.168 ± 0.633
5.113AlaLys: 5.113 ± 0.541
11.837AlaLeu: 11.837 ± 0.833
3.279AlaMet: 3.279 ± 0.386
3.446AlaAsn: 3.446 ± 0.479
6.724AlaPro: 6.724 ± 0.648
4.112AlaGln: 4.112 ± 0.541
9.059AlaArg: 9.059 ± 0.814
5.113AlaSer: 5.113 ± 0.462
6.669AlaThr: 6.669 ± 0.979
9.059AlaVal: 9.059 ± 0.846
2.39AlaTrp: 2.39 ± 0.285
2.723AlaTyr: 2.723 ± 0.382
0.056AlaXaa: 0.056 ± 0.056
Cys
1.445CysAla: 1.445 ± 0.296
0.111CysCys: 0.111 ± 0.066
1.0CysAsp: 1.0 ± 0.252
1.056CysGlu: 1.056 ± 0.26
0.222CysPhe: 0.222 ± 0.118
1.501CysGly: 1.501 ± 0.343
0.167CysHis: 0.167 ± 0.114
0.278CysIle: 0.278 ± 0.106
0.333CysLys: 0.333 ± 0.132
0.5CysLeu: 0.5 ± 0.162
0.278CysMet: 0.278 ± 0.125
0.278CysAsn: 0.278 ± 0.115
0.778CysPro: 0.778 ± 0.261
0.111CysGln: 0.111 ± 0.084
0.834CysArg: 0.834 ± 0.196
1.0CysSer: 1.0 ± 0.215
0.611CysThr: 0.611 ± 0.212
0.611CysVal: 0.611 ± 0.169
0.445CysTrp: 0.445 ± 0.16
0.278CysTyr: 0.278 ± 0.143
0.0CysXaa: 0.0 ± 0.0
Asp
8.058AspAla: 8.058 ± 0.695
0.834AspCys: 0.834 ± 0.238
5.946AspAsp: 5.946 ± 0.745
5.391AspGlu: 5.391 ± 0.523
1.111AspPhe: 1.111 ± 0.279
6.947AspGly: 6.947 ± 0.564
1.167AspHis: 1.167 ± 0.296
1.612AspIle: 1.612 ± 0.226
1.778AspLys: 1.778 ± 0.369
5.891AspLeu: 5.891 ± 0.69
1.778AspMet: 1.778 ± 0.329
1.556AspAsn: 1.556 ± 0.36
3.779AspPro: 3.779 ± 0.529
2.501AspGln: 2.501 ± 0.347
4.779AspArg: 4.779 ± 0.461
2.779AspSer: 2.779 ± 0.371
2.501AspThr: 2.501 ± 0.316
4.446AspVal: 4.446 ± 0.458
0.945AspTrp: 0.945 ± 0.209
1.501AspTyr: 1.501 ± 0.336
0.0AspXaa: 0.0 ± 0.0
Glu
7.614GluAla: 7.614 ± 0.8
1.056GluCys: 1.056 ± 0.232
3.279GluAsp: 3.279 ± 0.481
1.0GluGlu: 1.0 ± 0.272
1.501GluPhe: 1.501 ± 0.24
3.223GluGly: 3.223 ± 0.433
1.945GluHis: 1.945 ± 0.335
1.556GluIle: 1.556 ± 0.274
1.501GluLys: 1.501 ± 0.303
6.058GluLeu: 6.058 ± 0.673
1.334GluMet: 1.334 ± 0.255
1.0GluAsn: 1.0 ± 0.194
3.501GluPro: 3.501 ± 0.451
3.279GluGln: 3.279 ± 0.478
6.28GluArg: 6.28 ± 0.691
2.501GluSer: 2.501 ± 0.39
2.612GluThr: 2.612 ± 0.355
6.002GluVal: 6.002 ± 0.758
1.667GluTrp: 1.667 ± 0.317
1.612GluTyr: 1.612 ± 0.403
0.0GluXaa: 0.0 ± 0.0
Phe
2.945PheAla: 2.945 ± 0.438
0.333PheCys: 0.333 ± 0.129
2.89PheAsp: 2.89 ± 0.402
1.278PheGlu: 1.278 ± 0.182
0.778PhePhe: 0.778 ± 0.257
3.057PheGly: 3.057 ± 0.438
0.389PheHis: 0.389 ± 0.132
1.111PheIle: 1.111 ± 0.235
1.334PheLys: 1.334 ± 0.313
2.167PheLeu: 2.167 ± 0.315
0.722PheMet: 0.722 ± 0.222
1.111PheAsn: 1.111 ± 0.287
1.056PhePro: 1.056 ± 0.226
0.834PheGln: 0.834 ± 0.185
1.501PheArg: 1.501 ± 0.305
1.334PheSer: 1.334 ± 0.303
1.778PheThr: 1.778 ± 0.355
2.001PheVal: 2.001 ± 0.301
0.389PheTrp: 0.389 ± 0.137
0.5PheTyr: 0.5 ± 0.16
0.0PheXaa: 0.0 ± 0.0
Gly
9.725GlyAla: 9.725 ± 0.988
1.0GlyCys: 1.0 ± 0.262
4.502GlyAsp: 4.502 ± 0.55
6.224GlyGlu: 6.224 ± 0.495
2.334GlyPhe: 2.334 ± 0.376
8.169GlyGly: 8.169 ± 1.026
1.945GlyHis: 1.945 ± 0.367
2.279GlyIle: 2.279 ± 0.603
4.39GlyLys: 4.39 ± 0.52
7.447GlyLeu: 7.447 ± 0.762
1.612GlyMet: 1.612 ± 0.328
2.89GlyAsn: 2.89 ± 0.373
3.557GlyPro: 3.557 ± 0.552
2.39GlyGln: 2.39 ± 0.41
5.391GlyArg: 5.391 ± 0.698
5.057GlySer: 5.057 ± 0.672
5.669GlyThr: 5.669 ± 0.58
7.503GlyVal: 7.503 ± 0.589
2.056GlyTrp: 2.056 ± 0.303
2.334GlyTyr: 2.334 ± 0.328
0.0GlyXaa: 0.0 ± 0.0
His
2.279HisAla: 2.279 ± 0.397
0.389HisCys: 0.389 ± 0.137
1.556HisAsp: 1.556 ± 0.375
1.445HisGlu: 1.445 ± 0.337
0.611HisPhe: 0.611 ± 0.192
2.112HisGly: 2.112 ± 0.35
0.556HisHis: 0.556 ± 0.184
0.834HisIle: 0.834 ± 0.178
0.445HisLys: 0.445 ± 0.141
1.834HisLeu: 1.834 ± 0.312
0.722HisMet: 0.722 ± 0.225
0.556HisAsn: 0.556 ± 0.185
1.612HisPro: 1.612 ± 0.35
0.611HisGln: 0.611 ± 0.165
1.945HisArg: 1.945 ± 0.344
0.834HisSer: 0.834 ± 0.217
1.111HisThr: 1.111 ± 0.321
2.279HisVal: 2.279 ± 0.362
0.333HisTrp: 0.333 ± 0.167
0.722HisTyr: 0.722 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
5.891IleAla: 5.891 ± 0.548
0.333IleCys: 0.333 ± 0.134
3.334IleAsp: 3.334 ± 0.401
3.112IleGlu: 3.112 ± 0.482
0.389IlePhe: 0.389 ± 0.156
4.001IleGly: 4.001 ± 0.769
0.222IleHis: 0.222 ± 0.1
0.889IleIle: 0.889 ± 0.194
1.223IleLys: 1.223 ± 0.22
2.834IleLeu: 2.834 ± 0.383
0.389IleMet: 0.389 ± 0.133
1.223IleAsn: 1.223 ± 0.265
1.89IlePro: 1.89 ± 0.404
1.056IleGln: 1.056 ± 0.244
2.501IleArg: 2.501 ± 0.338
1.723IleSer: 1.723 ± 0.381
2.056IleThr: 2.056 ± 0.369
3.446IleVal: 3.446 ± 0.434
0.778IleTrp: 0.778 ± 0.179
0.333IleTyr: 0.333 ± 0.133
0.0IleXaa: 0.0 ± 0.0
Lys
4.335LysAla: 4.335 ± 0.483
0.278LysCys: 0.278 ± 0.125
1.278LysAsp: 1.278 ± 0.247
0.722LysGlu: 0.722 ± 0.158
1.389LysPhe: 1.389 ± 0.3
3.39LysGly: 3.39 ± 0.416
0.667LysHis: 0.667 ± 0.199
1.667LysIle: 1.667 ± 0.311
0.445LysLys: 0.445 ± 0.148
3.557LysLeu: 3.557 ± 0.401
0.722LysMet: 0.722 ± 0.179
0.556LysAsn: 0.556 ± 0.167
2.39LysPro: 2.39 ± 0.396
1.0LysGln: 1.0 ± 0.215
3.168LysArg: 3.168 ± 0.432
1.334LysSer: 1.334 ± 0.249
2.334LysThr: 2.334 ± 0.321
3.057LysVal: 3.057 ± 0.361
0.889LysTrp: 0.889 ± 0.212
0.667LysTyr: 0.667 ± 0.231
0.0LysXaa: 0.0 ± 0.0
Leu
12.282LeuAla: 12.282 ± 0.876
0.722LeuCys: 0.722 ± 0.223
7.058LeuAsp: 7.058 ± 0.677
2.223LeuGlu: 2.223 ± 0.332
2.723LeuPhe: 2.723 ± 0.364
6.613LeuGly: 6.613 ± 0.571
1.89LeuHis: 1.89 ± 0.355
3.057LeuIle: 3.057 ± 0.452
2.556LeuLys: 2.556 ± 0.372
5.613LeuLeu: 5.613 ± 0.566
1.501LeuMet: 1.501 ± 0.243
2.279LeuAsn: 2.279 ± 0.353
5.057LeuPro: 5.057 ± 0.744
2.834LeuGln: 2.834 ± 0.37
7.336LeuArg: 7.336 ± 0.694
5.057LeuSer: 5.057 ± 0.514
5.113LeuThr: 5.113 ± 0.457
6.78LeuVal: 6.78 ± 0.575
1.723LeuTrp: 1.723 ± 0.38
2.056LeuTyr: 2.056 ± 0.311
0.0LeuXaa: 0.0 ± 0.0
Met
2.834MetAla: 2.834 ± 0.39
0.278MetCys: 0.278 ± 0.139
0.667MetAsp: 0.667 ± 0.147
0.667MetGlu: 0.667 ± 0.197
0.778MetPhe: 0.778 ± 0.205
1.056MetGly: 1.056 ± 0.206
0.667MetHis: 0.667 ± 0.194
1.167MetIle: 1.167 ± 0.287
0.389MetLys: 0.389 ± 0.143
1.612MetLeu: 1.612 ± 0.348
0.333MetMet: 0.333 ± 0.125
0.834MetAsn: 0.834 ± 0.222
1.612MetPro: 1.612 ± 0.293
0.889MetGln: 0.889 ± 0.241
1.612MetArg: 1.612 ± 0.262
2.279MetSer: 2.279 ± 0.262
1.89MetThr: 1.89 ± 0.369
1.389MetVal: 1.389 ± 0.301
0.445MetTrp: 0.445 ± 0.168
0.333MetTyr: 0.333 ± 0.162
0.0MetXaa: 0.0 ± 0.0
Asn
3.946AsnAla: 3.946 ± 0.431
0.333AsnCys: 0.333 ± 0.121
1.278AsnAsp: 1.278 ± 0.252
1.167AsnGlu: 1.167 ± 0.308
0.945AsnPhe: 0.945 ± 0.267
3.501AsnGly: 3.501 ± 0.517
0.556AsnHis: 0.556 ± 0.228
0.889AsnIle: 0.889 ± 0.271
0.722AsnLys: 0.722 ± 0.204
1.778AsnLeu: 1.778 ± 0.365
0.611AsnMet: 0.611 ± 0.178
0.722AsnAsn: 0.722 ± 0.266
2.223AsnPro: 2.223 ± 0.436
0.611AsnGln: 0.611 ± 0.169
1.723AsnArg: 1.723 ± 0.291
1.0AsnSer: 1.0 ± 0.212
1.556AsnThr: 1.556 ± 0.317
2.39AsnVal: 2.39 ± 0.341
0.445AsnTrp: 0.445 ± 0.133
0.5AsnTyr: 0.5 ± 0.137
0.0AsnXaa: 0.0 ± 0.0
Pro
7.558ProAla: 7.558 ± 0.581
0.5ProCys: 0.5 ± 0.233
3.723ProAsp: 3.723 ± 0.428
4.613ProGlu: 4.613 ± 0.541
1.445ProPhe: 1.445 ± 0.239
5.502ProGly: 5.502 ± 0.529
1.278ProHis: 1.278 ± 0.298
2.279ProIle: 2.279 ± 0.279
2.001ProLys: 2.001 ± 0.33
4.224ProLeu: 4.224 ± 0.462
1.056ProMet: 1.056 ± 0.256
1.056ProAsn: 1.056 ± 0.295
2.834ProPro: 2.834 ± 0.429
1.445ProGln: 1.445 ± 0.31
3.001ProArg: 3.001 ± 0.481
3.612ProSer: 3.612 ± 0.586
2.945ProThr: 2.945 ± 0.397
4.335ProVal: 4.335 ± 0.511
1.167ProTrp: 1.167 ± 0.253
1.445ProTyr: 1.445 ± 0.331
0.0ProXaa: 0.0 ± 0.0
Gln
4.613GlnAla: 4.613 ± 0.587
0.278GlnCys: 0.278 ± 0.142
0.945GlnAsp: 0.945 ± 0.205
0.445GlnGlu: 0.445 ± 0.156
1.278GlnPhe: 1.278 ± 0.318
2.723GlnGly: 2.723 ± 0.27
1.0GlnHis: 1.0 ± 0.263
2.612GlnIle: 2.612 ± 0.393
0.722GlnLys: 0.722 ± 0.193
2.612GlnLeu: 2.612 ± 0.328
1.0GlnMet: 1.0 ± 0.227
0.778GlnAsn: 0.778 ± 0.216
2.056GlnPro: 2.056 ± 0.403
1.723GlnGln: 1.723 ± 0.323
2.39GlnArg: 2.39 ± 0.379
1.612GlnSer: 1.612 ± 0.269
1.723GlnThr: 1.723 ± 0.384
2.945GlnVal: 2.945 ± 0.341
1.056GlnTrp: 1.056 ± 0.193
0.889GlnTyr: 0.889 ± 0.161
0.0GlnXaa: 0.0 ± 0.0
Arg
6.502ArgAla: 6.502 ± 0.775
1.445ArgCys: 1.445 ± 0.305
3.89ArgAsp: 3.89 ± 0.531
5.669ArgGlu: 5.669 ± 0.642
2.056ArgPhe: 2.056 ± 0.316
5.28ArgGly: 5.28 ± 0.548
1.445ArgHis: 1.445 ± 0.325
3.112ArgIle: 3.112 ± 0.437
3.223ArgLys: 3.223 ± 0.437
6.335ArgLeu: 6.335 ± 0.655
2.223ArgMet: 2.223 ± 0.385
2.279ArgAsn: 2.279 ± 0.336
3.835ArgPro: 3.835 ± 0.466
2.223ArgGln: 2.223 ± 0.31
6.335ArgArg: 6.335 ± 0.77
3.279ArgSer: 3.279 ± 0.413
4.557ArgThr: 4.557 ± 0.657
4.779ArgVal: 4.779 ± 0.547
1.89ArgTrp: 1.89 ± 0.327
1.723ArgTyr: 1.723 ± 0.279
0.0ArgXaa: 0.0 ± 0.0
Ser
6.28SerAla: 6.28 ± 0.771
0.389SerCys: 0.389 ± 0.129
3.279SerAsp: 3.279 ± 0.328
2.556SerGlu: 2.556 ± 0.404
1.667SerPhe: 1.667 ± 0.316
4.668SerGly: 4.668 ± 0.718
1.723SerHis: 1.723 ± 0.376
1.834SerIle: 1.834 ± 0.317
1.667SerLys: 1.667 ± 0.264
4.279SerLeu: 4.279 ± 0.606
1.334SerMet: 1.334 ± 0.31
1.223SerAsn: 1.223 ± 0.264
2.834SerPro: 2.834 ± 0.339
1.834SerGln: 1.834 ± 0.33
2.779SerArg: 2.779 ± 0.466
2.834SerSer: 2.834 ± 0.503
3.001SerThr: 3.001 ± 0.272
3.39SerVal: 3.39 ± 0.51
1.334SerTrp: 1.334 ± 0.248
1.056SerTyr: 1.056 ± 0.221
0.0SerXaa: 0.0 ± 0.0
Thr
6.891ThrAla: 6.891 ± 0.678
0.945ThrCys: 0.945 ± 0.238
3.279ThrAsp: 3.279 ± 0.412
3.112ThrGlu: 3.112 ± 0.388
1.945ThrPhe: 1.945 ± 0.336
5.391ThrGly: 5.391 ± 0.551
1.445ThrHis: 1.445 ± 0.34
2.945ThrIle: 2.945 ± 0.485
1.612ThrLys: 1.612 ± 0.242
4.557ThrLeu: 4.557 ± 0.416
0.778ThrMet: 0.778 ± 0.254
1.445ThrAsn: 1.445 ± 0.251
3.612ThrPro: 3.612 ± 0.573
1.445ThrGln: 1.445 ± 0.261
3.168ThrArg: 3.168 ± 0.416
3.168ThrSer: 3.168 ± 0.505
3.557ThrThr: 3.557 ± 0.425
5.446ThrVal: 5.446 ± 0.599
0.945ThrTrp: 0.945 ± 0.219
1.834ThrTyr: 1.834 ± 0.306
0.0ThrXaa: 0.0 ± 0.0
Val
10.504ValAla: 10.504 ± 0.888
1.0ValCys: 1.0 ± 0.208
5.835ValAsp: 5.835 ± 0.659
6.113ValGlu: 6.113 ± 0.72
1.89ValPhe: 1.89 ± 0.338
6.391ValGly: 6.391 ± 0.718
2.167ValHis: 2.167 ± 0.391
3.168ValIle: 3.168 ± 0.408
3.446ValLys: 3.446 ± 0.44
6.502ValLeu: 6.502 ± 0.566
1.334ValMet: 1.334 ± 0.271
2.223ValAsn: 2.223 ± 0.317
4.724ValPro: 4.724 ± 0.476
1.834ValGln: 1.834 ± 0.312
3.89ValArg: 3.89 ± 0.502
3.612ValSer: 3.612 ± 0.418
4.779ValThr: 4.779 ± 0.474
6.058ValVal: 6.058 ± 0.68
1.945ValTrp: 1.945 ± 0.275
1.612ValTyr: 1.612 ± 0.243
0.0ValXaa: 0.0 ± 0.0
Trp
2.445TrpAla: 2.445 ± 0.354
0.278TrpCys: 0.278 ± 0.153
1.056TrpAsp: 1.056 ± 0.239
0.722TrpGlu: 0.722 ± 0.206
0.722TrpPhe: 0.722 ± 0.174
1.389TrpGly: 1.389 ± 0.293
0.722TrpHis: 0.722 ± 0.172
0.834TrpIle: 0.834 ± 0.188
0.333TrpLys: 0.333 ± 0.121
3.001TrpLeu: 3.001 ± 0.391
0.333TrpMet: 0.333 ± 0.12
0.611TrpAsn: 0.611 ± 0.16
0.889TrpPro: 0.889 ± 0.202
1.778TrpGln: 1.778 ± 0.304
2.612TrpArg: 2.612 ± 0.397
0.778TrpSer: 0.778 ± 0.211
1.223TrpThr: 1.223 ± 0.238
1.056TrpVal: 1.056 ± 0.223
0.389TrpTrp: 0.389 ± 0.142
0.5TrpTyr: 0.5 ± 0.18
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.945TyrAla: 2.945 ± 0.432
0.333TyrCys: 0.333 ± 0.151
1.834TyrAsp: 1.834 ± 0.342
1.445TyrGlu: 1.445 ± 0.302
0.611TyrPhe: 0.611 ± 0.172
2.223TyrGly: 2.223 ± 0.399
0.167TyrHis: 0.167 ± 0.081
0.333TyrIle: 0.333 ± 0.135
0.611TyrLys: 0.611 ± 0.182
1.834TyrLeu: 1.834 ± 0.309
0.333TyrMet: 0.333 ± 0.124
0.834TyrAsn: 0.834 ± 0.216
1.056TyrPro: 1.056 ± 0.212
0.889TyrGln: 0.889 ± 0.236
1.723TyrArg: 1.723 ± 0.317
1.111TyrSer: 1.111 ± 0.212
1.834TyrThr: 1.834 ± 0.321
2.056TyrVal: 2.056 ± 0.337
0.445TyrTrp: 0.445 ± 0.138
0.5TyrTyr: 0.5 ± 0.146
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.056XaaArg: 0.056 ± 0.056
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (17995 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski