Xylaria grammica

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Xylariaceae; Xylaria

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A439DAS4|A0A439DAS4_9PEZI Peptidase A1 domain-containing protein OS=Xylaria grammica OX=363999 GN=EKO27_g3571 PE=4 SV=1
MM1 pKa = 7.22SLSMSPLWLSVDD13 pKa = 3.51YY14 pKa = 11.29CDD16 pKa = 4.64INVTITTQADD26 pKa = 3.6IEE28 pKa = 4.58SLHH31 pKa = 5.12ATPYY35 pKa = 9.66EE36 pKa = 4.06FCFNNVTITNAEE48 pKa = 3.94GTLTFDD54 pKa = 5.06NFTQVNGLYY63 pKa = 10.45VQDD66 pKa = 4.23SPNLEE71 pKa = 3.89ALSFPDD77 pKa = 4.62LGRR80 pKa = 11.84LSALGVYY87 pKa = 9.81DD88 pKa = 5.63ALALSNISLPEE99 pKa = 3.87IQSVGLLPFGGGGWSLPPPWPGTEE123 pKa = 3.84DD124 pKa = 3.35RR125 pKa = 11.84SLIGVTINNAPALKK139 pKa = 9.79TIDD142 pKa = 3.9FDD144 pKa = 4.11FLSGFFTLEE153 pKa = 3.72LVGADD158 pKa = 3.49GLTDD162 pKa = 4.38FGPTGYY168 pKa = 10.62DD169 pKa = 2.86EE170 pKa = 5.99GILRR174 pKa = 11.84RR175 pKa = 11.84INSSDD180 pKa = 3.27TLSVDD185 pKa = 3.36GCFNLANVQFARR197 pKa = 11.84FVQITGRR204 pKa = 11.84TDD206 pKa = 3.17CEE208 pKa = 3.9YY209 pKa = 11.73LMLNYY214 pKa = 9.99HH215 pKa = 5.83SAYY218 pKa = 10.67NLTLIDD224 pKa = 3.85TAHH227 pKa = 6.3SRR229 pKa = 11.84LDD231 pKa = 3.17ILAPFAVNGTLVADD245 pKa = 4.35SLQLSPEE252 pKa = 4.09NSSSYY257 pKa = 11.01DD258 pKa = 3.44PQPLYY263 pKa = 10.69FISSIGEE270 pKa = 4.03DD271 pKa = 3.04AHH273 pKa = 7.01LLSGANVDD281 pKa = 3.91LSLDD285 pKa = 3.64EE286 pKa = 4.78VEE288 pKa = 4.55TLGGSLVASNNKK300 pKa = 9.25NCTFSFDD307 pKa = 3.56KK308 pKa = 10.96LSEE311 pKa = 4.25VKK313 pKa = 10.86GNISMTDD320 pKa = 3.42NPDD323 pKa = 3.23SALPWFPDD331 pKa = 3.14LRR333 pKa = 11.84RR334 pKa = 11.84AANIEE339 pKa = 3.62LRR341 pKa = 11.84GNINTSHH348 pKa = 6.64GPNIFPALTTVPGTVTIEE366 pKa = 3.62AWNSDD371 pKa = 4.19FNCSQLVKK379 pKa = 10.6QMQAGIIQNLVCNGTDD395 pKa = 3.3GTQGDD400 pKa = 4.32TSGSPSSPAGGAWTILGVAVGIMVLQNTIPVWW432 pKa = 3.47

Molecular weight: 46.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A439CZ16|A0A439CZ16_9PEZI Uncharacterized protein OS=Xylaria grammica OX=363999 GN=EKO27_g7890 PE=4 SV=1
MM1 pKa = 7.39LVGNATAAVAAVAAVAAVVAAVVAAAAAAAAAAALSVAAKK41 pKa = 10.04KK42 pKa = 10.58IFGAFSLALLRR53 pKa = 11.84SFFLVFLLLLLRR65 pKa = 11.84GRR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84HH71 pKa = 4.71RR72 pKa = 3.66

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12125

0

12125

5972924

24

6647

492.6

54.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.676 ± 0.019

1.154 ± 0.008

5.852 ± 0.013

6.121 ± 0.02

3.702 ± 0.015

6.969 ± 0.02

2.386 ± 0.009

5.033 ± 0.016

4.617 ± 0.019

8.929 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.021 ± 0.008

3.75 ± 0.011

6.053 ± 0.025

3.883 ± 0.016

6.148 ± 0.019

8.134 ± 0.023

6.079 ± 0.017

6.168 ± 0.014

1.49 ± 0.009

2.833 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski