Laurel Lake virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Laulavirus; Laurel Lake laulavirus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9YNI6|A0A2K9YNI6_9VIRU ORF1 OS=Laurel Lake virus OX=2027354 PE=4 SV=1
MM1 pKa = 7.72SNQTNMTDD9 pKa = 3.21VTSDD13 pKa = 3.49YY14 pKa = 11.44LNLPRR19 pKa = 11.84EE20 pKa = 4.18LQSIVEE26 pKa = 4.04NNILEE31 pKa = 4.42AAGEE35 pKa = 4.13EE36 pKa = 4.18FLQSIKK42 pKa = 10.49LASEE46 pKa = 4.15LRR48 pKa = 11.84RR49 pKa = 11.84DD50 pKa = 4.24LEE52 pKa = 4.72SSTQPDD58 pKa = 3.42DD59 pKa = 3.72DD60 pKa = 3.91RR61 pKa = 11.84RR62 pKa = 11.84NLRR65 pKa = 11.84LIRR68 pKa = 11.84GRR70 pKa = 11.84LGMSLLTSSLEE81 pKa = 4.17CPDD84 pKa = 4.53DD85 pKa = 4.28SFISSMGIGYY95 pKa = 10.07CYY97 pKa = 10.41LNSITQEE104 pKa = 3.83FRR106 pKa = 11.84IKK108 pKa = 10.44ALKK111 pKa = 7.67THH113 pKa = 5.87YY114 pKa = 10.03VWPTLQEE121 pKa = 3.94LFSRR125 pKa = 11.84WEE127 pKa = 3.73THH129 pKa = 4.67MVSGFRR135 pKa = 11.84LYY137 pKa = 9.78KK138 pKa = 9.92TVLSGKK144 pKa = 8.54LTVLHH149 pKa = 6.66LSYY152 pKa = 10.92SAVKK156 pKa = 9.9GVEE159 pKa = 4.03FVPIEE164 pKa = 3.97MQNQCNYY171 pKa = 8.72CHH173 pKa = 6.63AVSKK177 pKa = 10.73SYY179 pKa = 8.86TWLRR183 pKa = 11.84YY184 pKa = 8.85PKK186 pKa = 9.28LTHH189 pKa = 7.34DD190 pKa = 4.49VNCKK194 pKa = 8.71HH195 pKa = 5.57TVKK198 pKa = 10.81DD199 pKa = 4.63LISTPLDD206 pKa = 3.37SMYY209 pKa = 11.22LEE211 pKa = 4.69LKK213 pKa = 10.67VGGLFSRR220 pKa = 11.84LRR222 pKa = 11.84SPSQSTPTTSRR233 pKa = 11.84KK234 pKa = 8.99RR235 pKa = 11.84SKK237 pKa = 10.88SITDD241 pKa = 3.53SKK243 pKa = 10.86NHH245 pKa = 6.74EE246 pKa = 4.28DD247 pKa = 3.49RR248 pKa = 11.84QVIKK252 pKa = 10.46FDD254 pKa = 3.88RR255 pKa = 11.84SVKK258 pKa = 10.83GEE260 pKa = 3.92GKK262 pKa = 10.22EE263 pKa = 3.9PDD265 pKa = 3.48MSRR268 pKa = 11.84DD269 pKa = 3.58LYY271 pKa = 11.22SVPKK275 pKa = 7.78PTEE278 pKa = 3.71IEE280 pKa = 4.21TMSDD284 pKa = 2.71HH285 pKa = 7.0LNNHH289 pKa = 6.38KK290 pKa = 10.93SGDD293 pKa = 3.65TFPYY297 pKa = 8.9VSVVGTEE304 pKa = 4.75DD305 pKa = 3.14EE306 pKa = 4.6TKK308 pKa = 10.56DD309 pKa = 3.48GRR311 pKa = 11.84LSRR314 pKa = 11.84LRR316 pKa = 11.84AFRR319 pKa = 11.84SSYY322 pKa = 10.62GKK324 pKa = 9.92EE325 pKa = 3.99NKK327 pKa = 9.81PEE329 pKa = 3.66MRR331 pKa = 11.84INGVILDD338 pKa = 4.15EE339 pKa = 4.64LEE341 pKa = 4.11GTIKK345 pKa = 10.5VSNIGDD351 pKa = 3.77YY352 pKa = 10.99VNKK355 pKa = 10.42EE356 pKa = 4.05NLNKK360 pKa = 9.24QQRR363 pKa = 11.84SEE365 pKa = 4.1NKK367 pKa = 9.28RR368 pKa = 11.84FRR370 pKa = 11.84PLNEE374 pKa = 3.73ASFKK378 pKa = 10.89KK379 pKa = 10.11PYY381 pKa = 10.04LQIARR386 pKa = 11.84ITGEE390 pKa = 4.29YY391 pKa = 9.1VPLMSSTSDD400 pKa = 3.3YY401 pKa = 10.74TEE403 pKa = 5.1LYY405 pKa = 7.81FTLEE409 pKa = 4.43DD410 pKa = 4.84GRR412 pKa = 11.84LLDD415 pKa = 3.66NQVIIQSNKK424 pKa = 10.11LPTNQNGVFEE434 pKa = 5.05LSCDD438 pKa = 3.62YY439 pKa = 10.89CINLSDD445 pKa = 4.77INQLSLKK452 pKa = 9.72YY453 pKa = 10.04FLSRR457 pKa = 11.84PIMKK461 pKa = 10.3EE462 pKa = 3.73GFQWGAVSLTIRR474 pKa = 11.84VSEE477 pKa = 4.1SDD479 pKa = 3.52TPYY482 pKa = 9.9LTPKK486 pKa = 9.31VEE488 pKa = 3.77AMAIVRR494 pKa = 11.84IPFSTLEE501 pKa = 4.14EE502 pKa = 3.99QSKK505 pKa = 11.23DD506 pKa = 3.34PDD508 pKa = 3.58HH509 pKa = 7.08ADD511 pKa = 3.15VVFTAKK517 pKa = 10.21QVDD520 pKa = 3.93KK521 pKa = 11.18FKK523 pKa = 10.87EE524 pKa = 4.07LYY526 pKa = 10.02RR527 pKa = 11.84AGDD530 pKa = 3.42VMDD533 pKa = 4.0IDD535 pKa = 3.63EE536 pKa = 4.94AKK538 pKa = 10.42RR539 pKa = 11.84EE540 pKa = 3.78RR541 pKa = 11.84STINSYY547 pKa = 11.0SKK549 pKa = 9.02STIRR553 pKa = 11.84GVVKK557 pKa = 10.81GEE559 pKa = 4.24AGPSHH564 pKa = 7.42LGNQSGWEE572 pKa = 3.99HH573 pKa = 6.49LKK575 pKa = 11.18GMVKK579 pKa = 10.02PRR581 pKa = 11.84VEE583 pKa = 4.19EE584 pKa = 4.44GQASISVASGSEE596 pKa = 4.0EE597 pKa = 3.72VDD599 pKa = 2.56IDD601 pKa = 3.98VPSTTRR607 pKa = 11.84EE608 pKa = 3.98EE609 pKa = 4.39YY610 pKa = 10.39EE611 pKa = 4.06KK612 pKa = 10.6QQEE615 pKa = 4.25LLRR618 pKa = 11.84QQFQQTFSDD627 pKa = 3.72TTEE630 pKa = 4.28EE631 pKa = 4.15IPRR634 pKa = 11.84SEE636 pKa = 4.69LNRR639 pKa = 11.84SSSPINSDD647 pKa = 3.28PLSSVEE653 pKa = 3.97EE654 pKa = 4.2SSPPRR659 pKa = 11.84SPIKK663 pKa = 10.53SAMKK667 pKa = 9.81KK668 pKa = 9.79PRR670 pKa = 11.84FIEE673 pKa = 4.32DD674 pKa = 3.58TLKK677 pKa = 11.08KK678 pKa = 10.01PDD680 pKa = 3.66TSDD683 pKa = 3.34VYY685 pKa = 11.27QFNN688 pKa = 3.79

Molecular weight: 78.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223PR01|A0A223PR01_9VIRU Replicase OS=Laurel Lake virus OX=2027354 PE=4 SV=1
MM1 pKa = 7.28TSALSVYY8 pKa = 9.78TMISAADD15 pKa = 3.58TTGVEE20 pKa = 4.25EE21 pKa = 4.65FSTLFAYY28 pKa = 10.03EE29 pKa = 4.11GFNPEE34 pKa = 4.44MIHH37 pKa = 6.02SHH39 pKa = 5.69FAKK42 pKa = 10.39IMTEE46 pKa = 3.7KK47 pKa = 10.87GIGEE51 pKa = 4.2MEE53 pKa = 4.37FVNDD57 pKa = 3.36MRR59 pKa = 11.84ALITLGAMKK68 pKa = 10.63GNYY71 pKa = 7.09TMKK74 pKa = 10.46NAGKK78 pKa = 9.69ISEE81 pKa = 4.29AGRR84 pKa = 11.84TKK86 pKa = 11.15ADD88 pKa = 3.35GLYY91 pKa = 10.46KK92 pKa = 10.24KK93 pKa = 10.94YY94 pKa = 11.2NMKK97 pKa = 10.31QGSLGGDD104 pKa = 3.12KK105 pKa = 10.56KK106 pKa = 11.08AVILPRR112 pKa = 11.84VLSAFPEE119 pKa = 4.34LTTKK123 pKa = 10.3VVLRR127 pKa = 11.84TPPRR131 pKa = 11.84DD132 pKa = 3.04FGTRR136 pKa = 11.84TTRR139 pKa = 11.84LPKK142 pKa = 10.13YY143 pKa = 9.25IKK145 pKa = 10.83NPVFPSLVPKK155 pKa = 9.43TLQGDD160 pKa = 3.9VAKK163 pKa = 9.97TFLWLYY169 pKa = 8.09TVYY172 pKa = 10.52SAEE175 pKa = 3.82QSLVISQEE183 pKa = 3.59KK184 pKa = 10.42DD185 pKa = 3.28FNAAFTAQKK194 pKa = 10.47QFVTIAFNSSVPDD207 pKa = 3.21EE208 pKa = 4.24TTRR211 pKa = 11.84IGMFKK216 pKa = 10.79AKK218 pKa = 10.7LEE220 pKa = 4.31DD221 pKa = 3.64LVQAISDD228 pKa = 3.89FKK230 pKa = 11.34DD231 pKa = 3.67FTDD234 pKa = 5.16GEE236 pKa = 4.84VPTNRR241 pKa = 11.84SDD243 pKa = 3.53ARR245 pKa = 11.84VARR248 pKa = 11.84DD249 pKa = 4.12AITTLL254 pKa = 3.5

Molecular weight: 28.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3326

254

2384

1108.7

126.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.788 ± 1.017

1.624 ± 0.485

6.133 ± 0.134

7.096 ± 0.426

4.63 ± 0.505

5.021 ± 0.247

2.195 ± 0.398

5.833 ± 0.328

7.817 ± 0.373

9.771 ± 0.656

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.277 ± 0.356

4.931 ± 0.347

3.578 ± 0.626

3.187 ± 0.39

4.901 ± 0.512

9.832 ± 0.816

6.133 ± 0.898

5.683 ± 0.316

0.992 ± 0.192

3.548 ± 0.077
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski