Prevotella sp. CAG:386

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6QYQ7|R6QYQ7_9BACT Transcriptional regulator AraC family OS=Prevotella sp. CAG:386 OX=1262925 GN=BN637_00306 PE=4 SV=1
MM1 pKa = 6.69MKK3 pKa = 10.19KK4 pKa = 9.85YY5 pKa = 10.89VCDD8 pKa = 3.47VCGWVYY14 pKa = 10.9DD15 pKa = 4.13PEE17 pKa = 5.19VGDD20 pKa = 4.11PEE22 pKa = 5.4GGIAPGTAFEE32 pKa = 5.97DD33 pKa = 4.28IPDD36 pKa = 3.74DD37 pKa = 4.06WVCPLCGVGKK47 pKa = 10.39DD48 pKa = 3.73EE49 pKa = 5.35FSPMDD54 pKa = 3.34

Molecular weight: 5.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6Q579|R6Q579_9BACT KHG/KDPG family aldolase/carbohydrate kinase PfkB family OS=Prevotella sp. CAG:386 OX=1262925 GN=BN637_01792 PE=4 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2132

0

2132

747993

29

2619

350.8

39.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.52 ± 0.045

1.335 ± 0.02

5.804 ± 0.032

6.18 ± 0.056

4.401 ± 0.037

6.975 ± 0.059

2.099 ± 0.023

6.587 ± 0.041

6.892 ± 0.049

8.718 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.028 ± 0.027

5.072 ± 0.048

3.506 ± 0.025

3.691 ± 0.029

4.435 ± 0.039

5.991 ± 0.04

5.539 ± 0.043

6.664 ± 0.044

1.235 ± 0.022

4.327 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski