Amino acid dipepetide frequency for Prevotella sp. CAG:386

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.266AlaAla: 6.266 ± 0.098	1.015AlaCys: 1.015 ± 0.037	4.59AlaAsp: 4.59 ± 0.087	4.973AlaGlu: 4.973 ± 0.078	3.202AlaPhe: 3.202 ± 0.072	5.146AlaGly: 5.146 ± 0.092	1.509AlaHis: 1.509 ± 0.043	5.0AlaIle: 5.0 ± 0.103	5.148AlaLys: 5.148 ± 0.09	6.757AlaLeu: 6.757 ± 0.11	2.172AlaMet: 2.172 ± 0.057	3.517AlaAsn: 3.517 ± 0.088	2.269AlaPro: 2.269 ± 0.069	3.197AlaGln: 3.197 ± 0.069	3.111AlaArg: 3.111 ± 0.075	4.535AlaSer: 4.535 ± 0.084	4.07AlaThr: 4.07 ± 0.086	4.882AlaVal: 4.882 ± 0.089	0.87AlaTrp: 0.87 ± 0.032	2.968AlaTyr: 2.968 ± 0.074	0.001AlaXaa: 0.001 ± 0.001
Cys	0.83CysAla: 0.83 ± 0.036	0.249CysCys: 0.249 ± 0.02	0.773CysAsp: 0.773 ± 0.031	0.733CysGlu: 0.733 ± 0.032	0.602CysPhe: 0.602 ± 0.033	1.122CysGly: 1.122 ± 0.046	0.376CysHis: 0.376 ± 0.023	0.944CysIle: 0.944 ± 0.034	0.785CysLys: 0.785 ± 0.034	1.135CysLeu: 1.135 ± 0.04	0.353CysMet: 0.353 ± 0.02	0.634CysAsn: 0.634 ± 0.03	0.568CysPro: 0.568 ± 0.032	0.475CysGln: 0.475 ± 0.027	0.594CysArg: 0.594 ± 0.028	0.88CysSer: 0.88 ± 0.04	0.675CysThr: 0.675 ± 0.034	0.833CysVal: 0.833 ± 0.038	0.17CysTrp: 0.17 ± 0.015	0.622CysTyr: 0.622 ± 0.031	0.0CysXaa: 0.0 ± 0.0
Asp	4.398AspAla: 4.398 ± 0.086	0.746AspCys: 0.746 ± 0.034	3.234AspAsp: 3.234 ± 0.076	4.215AspGlu: 4.215 ± 0.091	3.079AspPhe: 3.079 ± 0.072	4.404AspGly: 4.404 ± 0.097	1.119AspHis: 1.119 ± 0.043	4.115AspIle: 4.115 ± 0.082	4.194AspLys: 4.194 ± 0.079	4.497AspLeu: 4.497 ± 0.084	1.806AspMet: 1.806 ± 0.051	3.067AspAsn: 3.067 ± 0.069	1.797AspPro: 1.797 ± 0.056	1.491AspGln: 1.491 ± 0.046	2.381AspArg: 2.381 ± 0.065	3.033AspSer: 3.033 ± 0.066	2.73AspThr: 2.73 ± 0.07	3.927AspVal: 3.927 ± 0.079	0.838AspTrp: 0.838 ± 0.034	2.967AspTyr: 2.967 ± 0.061	0.0AspXaa: 0.0 ± 0.0
Glu	4.943GluAla: 4.943 ± 0.091	0.664GluCys: 0.664 ± 0.035	3.364GluAsp: 3.364 ± 0.078	4.69GluGlu: 4.69 ± 0.102	2.353GluPhe: 2.353 ± 0.068	4.36GluGly: 4.36 ± 0.08	1.414GluHis: 1.414 ± 0.046	3.885GluIle: 3.885 ± 0.076	4.877GluLys: 4.877 ± 0.091	5.213GluLeu: 5.213 ± 0.09	2.087GluMet: 2.087 ± 0.055	3.221GluAsn: 3.221 ± 0.066	1.749GluPro: 1.749 ± 0.054	2.591GluGln: 2.591 ± 0.063	2.904GluArg: 2.904 ± 0.062	3.008GluSer: 3.008 ± 0.065	3.09GluThr: 3.09 ± 0.069	4.025GluVal: 4.025 ± 0.085	0.781GluTrp: 0.781 ± 0.033	2.583GluTyr: 2.583 ± 0.067	0.0GluXaa: 0.0 ± 0.0
Phe	3.361PheAla: 3.361 ± 0.062	0.782PheCys: 0.782 ± 0.029	3.019PheAsp: 3.019 ± 0.065	2.356PheGlu: 2.356 ± 0.058	2.063PhePhe: 2.063 ± 0.054	3.433PheGly: 3.433 ± 0.064	0.914PheHis: 0.914 ± 0.037	2.735PheIle: 2.735 ± 0.073	2.378PheLys: 2.378 ± 0.055	3.672PheLeu: 3.672 ± 0.081	1.295PheMet: 1.295 ± 0.046	2.166PheAsn: 2.166 ± 0.057	1.511PhePro: 1.511 ± 0.043	1.234PheGln: 1.234 ± 0.043	1.988PheArg: 1.988 ± 0.061	3.06PheSer: 3.06 ± 0.073	2.713PheThr: 2.713 ± 0.065	2.971PheVal: 2.971 ± 0.068	0.515PheTrp: 0.515 ± 0.028	1.845PheTyr: 1.845 ± 0.054	0.0PheXaa: 0.0 ± 0.0
Gly	4.571GlyAla: 4.571 ± 0.094	1.067GlyCys: 1.067 ± 0.043	3.675GlyAsp: 3.675 ± 0.082	4.265GlyGlu: 4.265 ± 0.077	3.278GlyPhe: 3.278 ± 0.075	4.977GlyGly: 4.977 ± 0.105	1.324GlyHis: 1.324 ± 0.048	5.267GlyIle: 5.267 ± 0.092	5.717GlyLys: 5.717 ± 0.107	5.658GlyLeu: 5.658 ± 0.092	2.239GlyMet: 2.239 ± 0.059	3.622GlyAsn: 3.622 ± 0.076	1.282GlyPro: 1.282 ± 0.048	2.179GlyGln: 2.179 ± 0.058	2.89GlyArg: 2.89 ± 0.075	4.27GlySer: 4.27 ± 0.088	4.225GlyThr: 4.225 ± 0.091	5.035GlyVal: 5.035 ± 0.094	0.979GlyTrp: 0.979 ± 0.043	3.229GlyTyr: 3.229 ± 0.073	0.0GlyXaa: 0.0 ± 0.0
His	1.333HisAla: 1.333 ± 0.04	0.361HisCys: 0.361 ± 0.027	1.207HisAsp: 1.207 ± 0.042	1.171HisGlu: 1.171 ± 0.043	1.066HisPhe: 1.066 ± 0.036	1.468HisGly: 1.468 ± 0.045	0.694HisHis: 0.694 ± 0.036	1.549HisIle: 1.549 ± 0.043	1.078HisLys: 1.078 ± 0.041	1.972HisLeu: 1.972 ± 0.059	0.389HisMet: 0.389 ± 0.024	1.004HisAsn: 1.004 ± 0.037	1.074HisPro: 1.074 ± 0.044	0.781HisGln: 0.781 ± 0.032	1.031HisArg: 1.031 ± 0.038	1.21HisSer: 1.21 ± 0.046	1.017HisThr: 1.017 ± 0.035	1.369HisVal: 1.369 ± 0.042	0.253HisTrp: 0.253 ± 0.017	0.96HisTyr: 0.96 ± 0.04	0.0HisXaa: 0.0 ± 0.0
Ile	5.346IleAla: 5.346 ± 0.092	0.987IleCys: 0.987 ± 0.041	4.389IleAsp: 4.389 ± 0.08	4.094IleGlu: 4.094 ± 0.079	2.698IlePhe: 2.698 ± 0.063	4.507IleGly: 4.507 ± 0.083	1.287IleHis: 1.287 ± 0.047	4.418IleIle: 4.418 ± 0.099	4.21IleLys: 4.21 ± 0.072	5.262IleLeu: 5.262 ± 0.104	1.627IleMet: 1.627 ± 0.044	3.369IleAsn: 3.369 ± 0.071	2.766IlePro: 2.766 ± 0.06	2.092IleGln: 2.092 ± 0.054	2.908IleArg: 2.908 ± 0.069	4.334IleSer: 4.334 ± 0.079	3.836IleThr: 3.836 ± 0.085	4.455IleVal: 4.455 ± 0.099	0.656IleTrp: 0.656 ± 0.029	2.632IleTyr: 2.632 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	5.656LysAla: 5.656 ± 0.094	0.574LysCys: 0.574 ± 0.029	4.195LysAsp: 4.195 ± 0.089	5.106LysGlu: 5.106 ± 0.097	2.348LysPhe: 2.348 ± 0.05	4.723LysGly: 4.723 ± 0.085	1.326LysHis: 1.326 ± 0.046	4.02LysIle: 4.02 ± 0.076	5.404LysLys: 5.404 ± 0.095	5.329LysLeu: 5.329 ± 0.085	2.576LysMet: 2.576 ± 0.058	3.701LysAsn: 3.701 ± 0.076	2.302LysPro: 2.302 ± 0.058	2.676LysGln: 2.676 ± 0.066	2.957LysArg: 2.957 ± 0.071	3.567LysSer: 3.567 ± 0.072	3.921LysThr: 3.921 ± 0.085	4.599LysVal: 4.599 ± 0.084	0.897LysTrp: 0.897 ± 0.03	3.037LysTyr: 3.037 ± 0.062	0.0LysXaa: 0.0 ± 0.0
Leu	6.531LeuAla: 6.531 ± 0.114	1.324LeuCys: 1.324 ± 0.046	4.782LeuAsp: 4.782 ± 0.08	4.283LeuGlu: 4.283 ± 0.077	3.872LeuPhe: 3.872 ± 0.075	5.809LeuGly: 5.809 ± 0.101	1.952LeuHis: 1.952 ± 0.059	4.844LeuIle: 4.844 ± 0.101	6.088LeuLys: 6.088 ± 0.088	8.151LeuLeu: 8.151 ± 0.141	2.726LeuMet: 2.726 ± 0.06	4.32LeuAsn: 4.32 ± 0.08	3.555LeuPro: 3.555 ± 0.062	3.475LeuGln: 3.475 ± 0.064	4.324LeuArg: 4.324 ± 0.088	6.419LeuSer: 6.419 ± 0.101	4.992LeuThr: 4.992 ± 0.079	5.334LeuVal: 5.334 ± 0.105	0.959LeuTrp: 0.959 ± 0.042	3.436LeuTyr: 3.436 ± 0.074	0.003LeuXaa: 0.003 ± 0.002
Met	2.54MetAla: 2.54 ± 0.064	0.283MetCys: 0.283 ± 0.019	1.57MetAsp: 1.57 ± 0.045	1.818MetGlu: 1.818 ± 0.049	1.045MetPhe: 1.045 ± 0.04	2.16MetGly: 2.16 ± 0.061	0.521MetHis: 0.521 ± 0.027	1.782MetIle: 1.782 ± 0.047	2.62MetLys: 2.62 ± 0.065	2.683MetLeu: 2.683 ± 0.065	1.142MetMet: 1.142 ± 0.039	1.634MetAsn: 1.634 ± 0.052	1.273MetPro: 1.273 ± 0.042	1.231MetGln: 1.231 ± 0.042	1.448MetArg: 1.448 ± 0.042	1.746MetSer: 1.746 ± 0.053	1.703MetThr: 1.703 ± 0.048	1.949MetVal: 1.949 ± 0.06	0.281MetTrp: 0.281 ± 0.019	0.853MetTyr: 0.853 ± 0.034	0.0MetXaa: 0.0 ± 0.0
Asn	3.675AsnAla: 3.675 ± 0.074	0.557AsnCys: 0.557 ± 0.031	2.87AsnAsp: 2.87 ± 0.068	2.767AsnGlu: 2.767 ± 0.054	2.198AsnPhe: 2.198 ± 0.058	3.861AsnGly: 3.861 ± 0.089	1.06AsnHis: 1.06 ± 0.036	3.719AsnIle: 3.719 ± 0.08	3.491AsnLys: 3.491 ± 0.079	4.205AsnLeu: 4.205 ± 0.084	1.35AsnMet: 1.35 ± 0.043	2.723AsnAsn: 2.723 ± 0.076	2.348AsnPro: 2.348 ± 0.057	1.738AsnGln: 1.738 ± 0.051	2.202AsnArg: 2.202 ± 0.049	2.82AsnSer: 2.82 ± 0.066	2.765AsnThr: 2.765 ± 0.074	3.361AsnVal: 3.361 ± 0.078	0.65AsnTrp: 0.65 ± 0.03	2.36AsnTyr: 2.36 ± 0.065	0.0AsnXaa: 0.0 ± 0.0
Pro	2.709ProAla: 2.709 ± 0.068	0.424ProCys: 0.424 ± 0.024	2.308ProAsp: 2.308 ± 0.057	2.861ProGlu: 2.861 ± 0.075	1.765ProPhe: 1.765 ± 0.052	2.33ProGly: 2.33 ± 0.064	0.713ProHis: 0.713 ± 0.033	2.183ProIle: 2.183 ± 0.05	1.98ProLys: 1.98 ± 0.055	2.913ProLeu: 2.913 ± 0.063	0.989ProMet: 0.989 ± 0.038	1.635ProAsn: 1.635 ± 0.046	0.626ProPro: 0.626 ± 0.03	1.437ProGln: 1.437 ± 0.053	1.241ProArg: 1.241 ± 0.04	2.14ProSer: 2.14 ± 0.056	2.024ProThr: 2.024 ± 0.051	2.743ProVal: 2.743 ± 0.068	0.441ProTrp: 0.441 ± 0.025	1.603ProTyr: 1.603 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	2.82GlnAla: 2.82 ± 0.072	0.377GlnCys: 0.377 ± 0.024	1.787GlnAsp: 1.787 ± 0.055	2.322GlnGlu: 2.322 ± 0.058	1.393GlnPhe: 1.393 ± 0.042	2.32GlnGly: 2.32 ± 0.056	0.754GlnHis: 0.754 ± 0.033	2.376GlnIle: 2.376 ± 0.057	2.604GlnLys: 2.604 ± 0.06	3.639GlnLeu: 3.639 ± 0.076	1.281GlnMet: 1.281 ± 0.045	1.828GlnAsn: 1.828 ± 0.054	1.293GlnPro: 1.293 ± 0.046	1.997GlnGln: 1.997 ± 0.06	1.725GlnArg: 1.725 ± 0.053	1.876GlnSer: 1.876 ± 0.05	2.028GlnThr: 2.028 ± 0.049	2.384GlnVal: 2.384 ± 0.053	0.488GlnTrp: 0.488 ± 0.023	1.623GlnTyr: 1.623 ± 0.053	0.0GlnXaa: 0.0 ± 0.0
Arg	2.687ArgAla: 2.687 ± 0.069	0.512ArgCys: 0.512 ± 0.027	2.211ArgAsp: 2.211 ± 0.066	2.731ArgGlu: 2.731 ± 0.075	2.108ArgPhe: 2.108 ± 0.062	2.566ArgGly: 2.566 ± 0.06	1.086ArgHis: 1.086 ± 0.043	3.202ArgIle: 3.202 ± 0.066	3.409ArgLys: 3.409 ± 0.071	4.245ArgLeu: 4.245 ± 0.086	1.583ArgMet: 1.583 ± 0.045	2.372ArgAsn: 2.372 ± 0.053	1.566ArgPro: 1.566 ± 0.052	2.124ArgGln: 2.124 ± 0.053	2.293ArgArg: 2.293 ± 0.067	2.215ArgSer: 2.215 ± 0.07	2.21ArgThr: 2.21 ± 0.056	2.562ArgVal: 2.562 ± 0.059	0.638ArgTrp: 0.638 ± 0.031	2.033ArgTyr: 2.033 ± 0.063	0.0ArgXaa: 0.0 ± 0.0
Ser	4.318SerAla: 4.318 ± 0.085	0.918SerCys: 0.918 ± 0.033	3.372SerAsp: 3.372 ± 0.07	3.217SerGlu: 3.217 ± 0.073	3.013SerPhe: 3.013 ± 0.073	4.13SerGly: 4.13 ± 0.091	1.358SerHis: 1.358 ± 0.042	4.086SerIle: 4.086 ± 0.072	3.635SerLys: 3.635 ± 0.075	5.771SerLeu: 5.771 ± 0.096	1.713SerMet: 1.713 ± 0.052	2.852SerAsn: 2.852 ± 0.07	2.029SerPro: 2.029 ± 0.046	2.123SerGln: 2.123 ± 0.057	2.636SerArg: 2.636 ± 0.061	3.808SerSer: 3.808 ± 0.097	3.21SerThr: 3.21 ± 0.074	4.178SerVal: 4.178 ± 0.081	0.775SerTrp: 0.775 ± 0.034	2.77SerTyr: 2.77 ± 0.079	0.001SerXaa: 0.001 ± 0.001
Thr	4.234ThrAla: 4.234 ± 0.097	0.635ThrCys: 0.635 ± 0.032	3.281ThrAsp: 3.281 ± 0.068	3.12ThrGlu: 3.12 ± 0.072	2.632ThrPhe: 2.632 ± 0.066	4.087ThrGly: 4.087 ± 0.077	1.12ThrHis: 1.12 ± 0.042	4.003ThrIle: 4.003 ± 0.077	3.039ThrLys: 3.039 ± 0.071	5.257ThrLeu: 5.257 ± 0.077	1.386ThrMet: 1.386 ± 0.046	2.516ThrAsn: 2.516 ± 0.068	2.623ThrPro: 2.623 ± 0.063	1.789ThrGln: 1.789 ± 0.044	2.06ThrArg: 2.06 ± 0.056	3.241ThrSer: 3.241 ± 0.078	3.38ThrThr: 3.38 ± 0.085	3.846ThrVal: 3.846 ± 0.084	0.683ThrTrp: 0.683 ± 0.033	2.452ThrTyr: 2.452 ± 0.065	0.003ThrXaa: 0.003 ± 0.002
Val	5.167ValAla: 5.167 ± 0.092	1.116ValCys: 1.116 ± 0.041	4.031ValAsp: 4.031 ± 0.077	4.171ValGlu: 4.171 ± 0.082	2.798ValPhe: 2.798 ± 0.065	4.348ValGly: 4.348 ± 0.098	1.138ValHis: 1.138 ± 0.038	4.239ValIle: 4.239 ± 0.083	4.793ValLys: 4.793 ± 0.082	5.862ValLeu: 5.862 ± 0.103	1.941ValMet: 1.941 ± 0.06	3.378ValAsn: 3.378 ± 0.076	2.465ValPro: 2.465 ± 0.058	1.906ValGln: 1.906 ± 0.056	2.902ValArg: 2.902 ± 0.073	4.432ValSer: 4.432 ± 0.079	3.691ValThr: 3.691 ± 0.096	4.902ValVal: 4.902 ± 0.102	0.765ValTrp: 0.765 ± 0.033	2.588ValTyr: 2.588 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.806TrpAla: 0.806 ± 0.033	0.176TrpCys: 0.176 ± 0.015	0.699TrpAsp: 0.699 ± 0.034	0.596TrpGlu: 0.596 ± 0.029	0.533TrpPhe: 0.533 ± 0.03	0.955TrpGly: 0.955 ± 0.035	0.305TrpHis: 0.305 ± 0.021	0.745TrpIle: 0.745 ± 0.036	0.85TrpLys: 0.85 ± 0.033	1.168TrpLeu: 1.168 ± 0.038	0.467TrpMet: 0.467 ± 0.023	0.809TrpAsn: 0.809 ± 0.039	0.299TrpPro: 0.299 ± 0.02	0.587TrpGln: 0.587 ± 0.028	0.599TrpArg: 0.599 ± 0.029	0.687TrpSer: 0.687 ± 0.03	0.672TrpThr: 0.672 ± 0.03	0.697TrpVal: 0.697 ± 0.032	0.176TrpTrp: 0.176 ± 0.016	0.519TrpTyr: 0.519 ± 0.03	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.007TyrAla: 3.007 ± 0.071	0.582TyrCys: 0.582 ± 0.031	2.682TyrAsp: 2.682 ± 0.066	2.31TyrGlu: 2.31 ± 0.057	1.965TyrPhe: 1.965 ± 0.052	3.044TyrGly: 3.044 ± 0.074	1.015TyrHis: 1.015 ± 0.036	2.761TyrIle: 2.761 ± 0.066	2.615TyrLys: 2.615 ± 0.064	3.785TyrLeu: 3.785 ± 0.071	1.159TyrMet: 1.159 ± 0.041	2.354TyrAsn: 2.354 ± 0.066	1.67TyrPro: 1.67 ± 0.05	1.79TyrGln: 1.79 ± 0.048	2.158TyrArg: 2.158 ± 0.061	2.631TyrSer: 2.631 ± 0.066	2.436TyrThr: 2.436 ± 0.071	2.584TyrVal: 2.584 ± 0.058	0.532TyrTrp: 0.532 ± 0.028	2.187TyrTyr: 2.187 ± 0.07	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.003XaaSer: 0.003 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.013XaaXaa: 0.013 ± 0.005

Statistics based on 2132 proteins (747993 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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