Torrubiella hemipterigena
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11466 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A1T6B4|A0A0A1T6B4_9HYPO Uncharacterized protein OS=Torrubiella hemipterigena OX=1531966 GN=VHEMI01956 PE=4 SV=1
MM1 pKa = 7.83 PSWQDD6 pKa = 3.34 LPTVRR11 pKa = 11.84 SDD13 pKa = 4.6 DD14 pKa = 4.66 DD15 pKa = 3.5 TDD17 pKa = 3.68 QDD19 pKa = 3.93 VNAVDD24 pKa = 4.39 NRR26 pKa = 11.84 AQFPIEE32 pKa = 4.15 TFGPDD37 pKa = 3.05 NTGGISNDD45 pKa = 3.05 LTVFSAHH52 pKa = 6.46 VDD54 pKa = 3.93 FDD56 pKa = 4.44 LDD58 pKa = 3.57 ATHH61 pKa = 7.37 LEE63 pKa = 4.09 FLGPEE68 pKa = 4.09 FVVVEE73 pKa = 4.21 MPDD76 pKa = 3.69 YY77 pKa = 10.73 PPHH80 pKa = 6.12 QDD82 pKa = 2.37 GRR84 pKa = 11.84 YY85 pKa = 7.19 YY86 pKa = 10.93 CRR88 pKa = 11.84 DD89 pKa = 3.54 AEE91 pKa = 4.36 CDD93 pKa = 3.54 PRR95 pKa = 11.84 PSFTTGSALKK105 pKa = 10.53 YY106 pKa = 8.76 YY107 pKa = 10.6 DD108 pKa = 4.69 PYY110 pKa = 11.88 SPMM113 pKa = 4.38
Molecular weight: 12.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.592
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.961
DTASelect 4.24
Thurlkill 3.668
EMBOSS 3.821
Sillero 3.948
Patrickios 0.998
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.841
Protein with the highest isoelectric point:
>tr|A0A0A1T084|A0A0A1T084_9HYPO FAD-binding PCMH-type domain-containing protein OS=Torrubiella hemipterigena OX=1531966 GN=VHEMI04053 PE=3 SV=1
MM1 pKa = 7.67 HH2 pKa = 7.42 ATQLHH7 pKa = 6.05 KK8 pKa = 10.36 MQALTRR14 pKa = 11.84 AARR17 pKa = 11.84 PVLRR21 pKa = 11.84 TTLPIRR27 pKa = 11.84 TFTTMTTPIRR37 pKa = 11.84 PSLSASAIRR46 pKa = 11.84 RR47 pKa = 11.84 PTSLLPSLLTPPPATTTAAAADD69 pKa = 4.03 VVSTSAISAHH79 pKa = 6.47 PAFAGAQMRR88 pKa = 11.84 FGPRR92 pKa = 11.84 NTMTGATRR100 pKa = 11.84 LVQKK104 pKa = 10.08 RR105 pKa = 11.84 RR106 pKa = 11.84 HH107 pKa = 4.96 GFLYY111 pKa = 10.42 RR112 pKa = 11.84 MRR114 pKa = 11.84 SRR116 pKa = 11.84 TGRR119 pKa = 11.84 KK120 pKa = 8.73 IILRR124 pKa = 11.84 RR125 pKa = 11.84 KK126 pKa = 7.15 TKK128 pKa = 9.84 GRR130 pKa = 11.84 RR131 pKa = 11.84 HH132 pKa = 5.06 VAWW135 pKa = 4.03
Molecular weight: 14.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.464
IPC2_protein 11.125
IPC_protein 12.603
Toseland 12.764
ProMoST 13.247
Dawson 12.764
Bjellqvist 12.749
Wikipedia 13.232
Rodwell 12.369
Grimsley 12.793
Solomon 13.261
Lehninger 13.159
Nozaki 12.764
DTASelect 12.749
Thurlkill 12.764
EMBOSS 13.261
Sillero 12.764
Patrickios 12.091
IPC_peptide 13.261
IPC2_peptide 12.252
IPC2.peptide.svr19 9.105
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10771
695
11466
5553574
50
7027
484.4
53.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.158 ± 0.022
1.274 ± 0.009
5.903 ± 0.018
5.828 ± 0.022
3.749 ± 0.013
6.742 ± 0.02
2.318 ± 0.01
5.097 ± 0.014
5.003 ± 0.018
8.82 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.349 ± 0.009
3.831 ± 0.011
5.717 ± 0.023
4.037 ± 0.016
5.676 ± 0.018
8.028 ± 0.025
6.082 ± 0.015
6.127 ± 0.016
1.455 ± 0.007
2.808 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here