Beauveria bassiana RNA virus 1
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G4DJ74|A0A0G4DJ74_9VIRU Uncharacterized protein OS=Beauveria bassiana RNA virus 1 OX=1054649 PE=4 SV=1
MM1 pKa = 6.68 MVDD4 pKa = 4.2 WIAEE8 pKa = 4.13 NPSTVSEE15 pKa = 4.04 ASGNGVKK22 pKa = 9.24 EE23 pKa = 3.44 WRR25 pKa = 11.84 FFLKK29 pKa = 9.91 HH30 pKa = 6.23 CSWQRR35 pKa = 11.84 RR36 pKa = 11.84 LPGVSSLGHH45 pKa = 5.73 SPAVRR50 pKa = 11.84 YY51 pKa = 9.85 RR52 pKa = 11.84 YY53 pKa = 8.14 VQPEE57 pKa = 4.15 TSPSFLFSKK66 pKa = 10.54 FLDD69 pKa = 3.89 EE70 pKa = 4.55 SQSSLGGIEE79 pKa = 4.24 GPARR83 pKa = 11.84 DD84 pKa = 3.65 VLFSEE89 pKa = 5.33 FVPPSEE95 pKa = 5.5 DD96 pKa = 4.26 DD97 pKa = 3.81 LFEE100 pKa = 5.86 HH101 pKa = 6.89 IAGFGEE107 pKa = 4.29 PGPDD111 pKa = 3.6 GSHH114 pKa = 7.08 LPGSDD119 pKa = 3.16 FAQLFRR125 pKa = 11.84 EE126 pKa = 4.41 LRR128 pKa = 11.84 RR129 pKa = 11.84 LEE131 pKa = 3.97 QWDD134 pKa = 3.97 VPISGLLDD142 pKa = 3.07 ATMLPKK148 pKa = 10.01 IRR150 pKa = 11.84 VPSATSPGIRR160 pKa = 11.84 WKK162 pKa = 10.87 KK163 pKa = 10.21 LGYY166 pKa = 7.66 KK167 pKa = 8.29 TKK169 pKa = 10.53 RR170 pKa = 11.84 QALVPAVVEE179 pKa = 4.07 ATRR182 pKa = 11.84 ALNRR186 pKa = 11.84 MVEE189 pKa = 4.19 EE190 pKa = 4.21 EE191 pKa = 4.1 VAYY194 pKa = 8.04 TVPPCGVAGRR204 pKa = 11.84 GKK206 pKa = 10.09 RR207 pKa = 11.84 VNILEE212 pKa = 4.3 GGSRR216 pKa = 11.84 KK217 pKa = 9.63 GKK219 pKa = 9.89 KK220 pKa = 9.68 DD221 pKa = 2.82 GRR223 pKa = 11.84 LIVMPDD229 pKa = 3.6 LVRR232 pKa = 11.84 HH233 pKa = 6.01 LLGSLGSAPYY243 pKa = 9.48 MSRR246 pKa = 11.84 LRR248 pKa = 11.84 GMDD251 pKa = 3.19 HH252 pKa = 6.84 SGGGVLLGMGPFQDD266 pKa = 3.9 QYY268 pKa = 11.86 QLIADD273 pKa = 4.41 WATGASAFCFIDD285 pKa = 4.23 FKK287 pKa = 11.43 KK288 pKa = 10.49 FDD290 pKa = 3.4 QRR292 pKa = 11.84 VPRR295 pKa = 11.84 RR296 pKa = 11.84 LLRR299 pKa = 11.84 GVMNHH304 pKa = 5.77 IRR306 pKa = 11.84 SAFIGEE312 pKa = 4.25 PGSRR316 pKa = 11.84 AYY318 pKa = 9.44 WEE320 pKa = 4.21 SEE322 pKa = 3.48 FRR324 pKa = 11.84 HH325 pKa = 6.12 LVDD328 pKa = 4.52 TEE330 pKa = 3.66 IAMPDD335 pKa = 3.75 GSCYY339 pKa = 10.27 RR340 pKa = 11.84 KK341 pKa = 9.89 RR342 pKa = 11.84 GCVASGDD349 pKa = 3.84 PWTSLAGSYY358 pKa = 11.07 ANWIILKK365 pKa = 9.69 WIFLKK370 pKa = 10.99 LGLEE374 pKa = 4.17 VKK376 pKa = 9.86 IWTFGDD382 pKa = 3.6 DD383 pKa = 3.6 SVVAIYY389 pKa = 10.07 GQVDD393 pKa = 3.23 TGGLMSQVVRR403 pKa = 11.84 LAWEE407 pKa = 4.42 GFGMNVSRR415 pKa = 11.84 EE416 pKa = 3.9 KK417 pKa = 11.02 SYY419 pKa = 8.88 VTCHH423 pKa = 6.28 LVDD426 pKa = 3.49 IADD429 pKa = 4.14 DD430 pKa = 4.28 PEE432 pKa = 4.18 PQKK435 pKa = 11.16 AGSFLSMYY443 pKa = 10.46 FLQTPMGVRR452 pKa = 11.84 PTRR455 pKa = 11.84 PLQDD459 pKa = 3.17 MYY461 pKa = 11.63 EE462 pKa = 4.17 LLLKK466 pKa = 10.31 PEE468 pKa = 4.17 KK469 pKa = 10.58 CRR471 pKa = 11.84 GTVEE475 pKa = 3.95 WEE477 pKa = 4.18 VVRR480 pKa = 11.84 TSMAYY485 pKa = 10.06 LVFYY489 pKa = 10.85 YY490 pKa = 10.74 NEE492 pKa = 3.69 TARR495 pKa = 11.84 YY496 pKa = 8.61 VLEE499 pKa = 4.81 EE500 pKa = 3.98 YY501 pKa = 9.48 WVWLHH506 pKa = 5.84 RR507 pKa = 11.84 VHH509 pKa = 7.84 RR510 pKa = 11.84 IPEE513 pKa = 4.15 LTGTADD519 pKa = 4.26 DD520 pKa = 3.87 MALLRR525 pKa = 11.84 EE526 pKa = 4.09 MDD528 pKa = 3.9 IPWSSFRR535 pKa = 11.84 MEE537 pKa = 4.1 WLNRR541 pKa = 11.84 LAFHH545 pKa = 7.15 GEE547 pKa = 3.82 VEE549 pKa = 4.15 LMYY552 pKa = 10.56 KK553 pKa = 10.23 YY554 pKa = 10.91 GHH556 pKa = 6.11 TKK558 pKa = 10.09 FFPPVLWNAWYY569 pKa = 9.16 QQYY572 pKa = 11.13 DD573 pKa = 3.5 EE574 pKa = 5.32 SICGNEE580 pKa = 3.76 IVPDD584 pKa = 3.54 QRR586 pKa = 11.84 APNDD590 pKa = 3.32
Molecular weight: 66.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.039
IPC2_protein 6.02
IPC_protein 6.097
Toseland 6.427
ProMoST 6.364
Dawson 6.402
Bjellqvist 6.389
Wikipedia 6.415
Rodwell 6.389
Grimsley 6.561
Solomon 6.402
Lehninger 6.389
Nozaki 6.664
DTASelect 6.854
Thurlkill 6.868
EMBOSS 6.839
Sillero 6.781
Patrickios 4.253
IPC_peptide 6.415
IPC2_peptide 6.693
IPC2.peptide.svr19 6.689
Protein with the highest isoelectric point:
>tr|A0A0G4DJ74|A0A0G4DJ74_9VIRU Uncharacterized protein OS=Beauveria bassiana RNA virus 1 OX=1054649 PE=4 SV=1
MM1 pKa = 6.34 MTPVARR7 pKa = 11.84 NQEE10 pKa = 4.13 RR11 pKa = 11.84 ADD13 pKa = 3.49 VSSVKK18 pKa = 10.12 EE19 pKa = 4.18 ATQSAPGTATNTGATEE35 pKa = 4.29 RR36 pKa = 11.84 PTSPAARR43 pKa = 11.84 RR44 pKa = 11.84 SSTPVRR50 pKa = 11.84 PQVPPDD56 pKa = 3.83 LEE58 pKa = 6.3 AGLTLNMVTTRR69 pKa = 11.84 TVPSHH74 pKa = 6.78 PEE76 pKa = 3.17 AVLRR80 pKa = 11.84 VLQAFLGARR89 pKa = 11.84 GVTTLPSSVRR99 pKa = 11.84 DD100 pKa = 3.74 LYY102 pKa = 10.48 PSNWRR107 pKa = 11.84 IGNTTIPVRR116 pKa = 11.84 GSPEE120 pKa = 3.68 SATRR124 pKa = 11.84 LGEE127 pKa = 4.14 VLPHH131 pKa = 6.19 LRR133 pKa = 11.84 GFNAKK138 pKa = 10.09 GEE140 pKa = 4.2 EE141 pKa = 4.09 VHH143 pKa = 6.77 WISEE147 pKa = 4.09 GRR149 pKa = 11.84 DD150 pKa = 3.2 ASSVVRR156 pKa = 11.84 LMAEE160 pKa = 3.38 WDD162 pKa = 3.92 SPVEE166 pKa = 3.56 IAAAILADD174 pKa = 3.9 VKK176 pKa = 11.21 DD177 pKa = 3.91 VDD179 pKa = 3.91 VSKK182 pKa = 11.49 DD183 pKa = 3.33 DD184 pKa = 3.79 VLAVAQRR191 pKa = 11.84 SPQALEE197 pKa = 3.72 QLKK200 pKa = 10.91 KK201 pKa = 10.29 SGKK204 pKa = 7.23 EE205 pKa = 3.86 FKK207 pKa = 10.47 EE208 pKa = 4.34 HH209 pKa = 4.16 VQRR212 pKa = 11.84 WLDD215 pKa = 3.6 PVALRR220 pKa = 11.84 RR221 pKa = 11.84 SPEE224 pKa = 4.05 FIAVSLRR231 pKa = 11.84 YY232 pKa = 9.12 KK233 pKa = 10.41 AQLRR237 pKa = 11.84 SLIDD241 pKa = 3.62 EE242 pKa = 4.05 QQKK245 pKa = 11.11 AIVAARR251 pKa = 11.84 KK252 pKa = 8.05 ATAAVNRR259 pKa = 11.84 ALGEE263 pKa = 3.87 RR264 pKa = 11.84 DD265 pKa = 3.08 EE266 pKa = 4.68 ALARR270 pKa = 11.84 LDD272 pKa = 3.4 PGYY275 pKa = 10.67 VPKK278 pKa = 10.74 RR279 pKa = 11.84 ATAARR284 pKa = 11.84 ALAEE288 pKa = 4.29 FGIDD292 pKa = 3.68 LDD294 pKa = 4.95 GDD296 pKa = 4.04 TEE298 pKa = 4.12 MDD300 pKa = 3.99 DD301 pKa = 3.83 AAGAASDD308 pKa = 4.21 ALRR311 pKa = 11.84 DD312 pKa = 3.52 LDD314 pKa = 4.03 FF315 pKa = 5.87
Molecular weight: 34.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.086
IPC2_protein 5.919
IPC_protein 5.97
Toseland 6.415
ProMoST 6.224
Dawson 6.173
Bjellqvist 6.173
Wikipedia 6.16
Rodwell 6.148
Grimsley 6.605
Solomon 6.173
Lehninger 6.16
Nozaki 6.402
DTASelect 6.605
Thurlkill 6.576
EMBOSS 6.547
Sillero 6.504
Patrickios 5.092
IPC_peptide 6.186
IPC2_peptide 6.453
IPC2.peptide.svr19 6.548
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
905
315
590
452.5
50.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.95 ± 2.987
0.884 ± 0.495
5.967 ± 0.57
6.63 ± 0.021
3.757 ± 1.037
7.624 ± 1.247
1.878 ± 0.341
3.536 ± 0.38
3.978 ± 0.094
9.171 ± 0.158
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.762 ± 0.658
2.099 ± 0.069
6.409 ± 0.144
3.204 ± 0.161
7.956 ± 0.7
7.293 ± 0.005
4.53 ± 1.018
8.066 ± 0.461
2.541 ± 0.712
2.762 ± 1.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here