Amino acid dipepetide frequency for Escherichia phage Sortsne

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.612AlaAla: 11.612 ± 1.749
0.852AlaCys: 0.852 ± 0.274
6.503AlaAsp: 6.503 ± 0.635
7.509AlaGlu: 7.509 ± 0.856
3.329AlaPhe: 3.329 ± 0.535
7.973AlaGly: 7.973 ± 1.123
2.09AlaHis: 2.09 ± 0.337
6.425AlaIle: 6.425 ± 0.656
5.187AlaLys: 5.187 ± 0.783
10.141AlaLeu: 10.141 ± 1.037
3.638AlaMet: 3.638 ± 0.582
3.793AlaAsn: 3.793 ± 0.634
3.329AlaPro: 3.329 ± 0.485
6.27AlaGln: 6.27 ± 1.164
7.354AlaArg: 7.354 ± 0.713
5.341AlaSer: 5.341 ± 0.658
5.187AlaThr: 5.187 ± 0.839
5.341AlaVal: 5.341 ± 0.658
1.471AlaTrp: 1.471 ± 0.335
3.484AlaTyr: 3.484 ± 0.574
0.0AlaXaa: 0.0 ± 0.0
Cys
0.619CysAla: 0.619 ± 0.205
0.155CysCys: 0.155 ± 0.111
1.084CysAsp: 1.084 ± 0.319
0.542CysGlu: 0.542 ± 0.213
0.542CysPhe: 0.542 ± 0.266
1.471CysGly: 1.471 ± 0.446
0.232CysHis: 0.232 ± 0.116
0.232CysIle: 0.232 ± 0.111
0.619CysLys: 0.619 ± 0.253
0.619CysLeu: 0.619 ± 0.199
0.387CysMet: 0.387 ± 0.218
0.464CysAsn: 0.464 ± 0.179
0.387CysPro: 0.387 ± 0.14
0.155CysGln: 0.155 ± 0.1
1.471CysArg: 1.471 ± 0.367
0.387CysSer: 0.387 ± 0.206
0.387CysThr: 0.387 ± 0.222
0.852CysVal: 0.852 ± 0.237
0.232CysTrp: 0.232 ± 0.157
0.31CysTyr: 0.31 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
6.425AspAla: 6.425 ± 0.81
0.542AspCys: 0.542 ± 0.21
4.18AspAsp: 4.18 ± 0.834
4.722AspGlu: 4.722 ± 0.688
1.78AspPhe: 1.78 ± 0.343
5.883AspGly: 5.883 ± 0.747
1.78AspHis: 1.78 ± 0.441
2.245AspIle: 2.245 ± 0.409
2.555AspLys: 2.555 ± 0.416
6.425AspLeu: 6.425 ± 0.537
1.006AspMet: 1.006 ± 0.249
2.709AspAsn: 2.709 ± 0.635
3.871AspPro: 3.871 ± 0.589
2.322AspGln: 2.322 ± 0.394
4.877AspArg: 4.877 ± 0.654
2.555AspSer: 2.555 ± 0.477
2.632AspThr: 2.632 ± 0.39
4.18AspVal: 4.18 ± 0.488
1.935AspTrp: 1.935 ± 0.333
1.935AspTyr: 1.935 ± 0.361
0.0AspXaa: 0.0 ± 0.0
Glu
9.522GluAla: 9.522 ± 0.914
0.852GluCys: 0.852 ± 0.308
3.871GluAsp: 3.871 ± 0.522
4.103GluGlu: 4.103 ± 0.858
1.703GluPhe: 1.703 ± 0.359
3.871GluGly: 3.871 ± 0.725
1.239GluHis: 1.239 ± 0.311
2.942GluIle: 2.942 ± 0.528
4.567GluLys: 4.567 ± 0.598
4.335GluLeu: 4.335 ± 0.65
1.703GluMet: 1.703 ± 0.361
2.322GluAsn: 2.322 ± 0.585
2.864GluPro: 2.864 ± 0.613
4.567GluGln: 4.567 ± 0.663
4.025GluArg: 4.025 ± 0.802
2.709GluSer: 2.709 ± 0.361
2.709GluThr: 2.709 ± 0.385
4.567GluVal: 4.567 ± 0.684
1.316GluTrp: 1.316 ± 0.299
2.4GluTyr: 2.4 ± 0.346
0.0GluXaa: 0.0 ± 0.0
Phe
2.787PheAla: 2.787 ± 0.577
0.155PheCys: 0.155 ± 0.103
2.245PheAsp: 2.245 ± 0.433
2.168PheGlu: 2.168 ± 0.382
0.852PhePhe: 0.852 ± 0.267
3.251PheGly: 3.251 ± 0.553
0.31PheHis: 0.31 ± 0.139
1.703PheIle: 1.703 ± 0.387
1.78PheLys: 1.78 ± 0.404
2.168PheLeu: 2.168 ± 0.439
1.006PheMet: 1.006 ± 0.266
1.703PheAsn: 1.703 ± 0.312
1.316PhePro: 1.316 ± 0.38
0.697PheGln: 0.697 ± 0.224
2.245PheArg: 2.245 ± 0.406
1.393PheSer: 1.393 ± 0.254
1.161PheThr: 1.161 ± 0.307
1.703PheVal: 1.703 ± 0.355
0.774PheTrp: 0.774 ± 0.279
0.464PheTyr: 0.464 ± 0.202
0.0PheXaa: 0.0 ± 0.0
Gly
7.586GlyAla: 7.586 ± 0.784
0.619GlyCys: 0.619 ± 0.274
3.638GlyAsp: 3.638 ± 0.552
5.264GlyGlu: 5.264 ± 0.627
2.787GlyPhe: 2.787 ± 0.466
7.741GlyGly: 7.741 ± 1.15
1.471GlyHis: 1.471 ± 0.342
4.103GlyIle: 4.103 ± 0.893
5.264GlyLys: 5.264 ± 0.803
5.419GlyLeu: 5.419 ± 0.653
1.703GlyMet: 1.703 ± 0.366
3.019GlyAsn: 3.019 ± 0.761
3.329GlyPro: 3.329 ± 0.467
2.942GlyGln: 2.942 ± 0.496
5.109GlyArg: 5.109 ± 0.611
4.954GlySer: 4.954 ± 0.649
3.871GlyThr: 3.871 ± 0.585
5.109GlyVal: 5.109 ± 0.766
1.084GlyTrp: 1.084 ± 0.275
2.864GlyTyr: 2.864 ± 0.54
0.0GlyXaa: 0.0 ± 0.0
His
1.626HisAla: 1.626 ± 0.384
0.464HisCys: 0.464 ± 0.178
1.393HisAsp: 1.393 ± 0.338
1.239HisGlu: 1.239 ± 0.319
0.619HisPhe: 0.619 ± 0.239
1.316HisGly: 1.316 ± 0.284
0.929HisHis: 0.929 ± 0.326
1.006HisIle: 1.006 ± 0.269
1.006HisLys: 1.006 ± 0.269
1.626HisLeu: 1.626 ± 0.347
0.31HisMet: 0.31 ± 0.137
0.542HisAsn: 0.542 ± 0.194
1.161HisPro: 1.161 ± 0.323
0.929HisGln: 0.929 ± 0.222
1.084HisArg: 1.084 ± 0.223
1.161HisSer: 1.161 ± 0.321
0.929HisThr: 0.929 ± 0.285
1.316HisVal: 1.316 ± 0.335
0.387HisTrp: 0.387 ± 0.159
0.387HisTyr: 0.387 ± 0.168
0.0HisXaa: 0.0 ± 0.0
Ile
4.8IleAla: 4.8 ± 0.584
0.619IleCys: 0.619 ± 0.219
3.716IleAsp: 3.716 ± 0.435
4.025IleGlu: 4.025 ± 0.51
1.626IlePhe: 1.626 ± 0.341
3.638IleGly: 3.638 ± 0.597
0.852IleHis: 0.852 ± 0.28
2.245IleIle: 2.245 ± 0.425
1.626IleLys: 1.626 ± 0.372
3.174IleLeu: 3.174 ± 0.582
1.471IleMet: 1.471 ± 0.313
1.703IleAsn: 1.703 ± 0.344
3.174IlePro: 3.174 ± 0.503
2.322IleGln: 2.322 ± 0.493
3.251IleArg: 3.251 ± 0.534
2.787IleSer: 2.787 ± 0.52
3.096IleThr: 3.096 ± 0.504
3.793IleVal: 3.793 ± 0.643
0.542IleTrp: 0.542 ± 0.204
1.626IleTyr: 1.626 ± 0.316
0.0IleXaa: 0.0 ± 0.0
Lys
5.651LysAla: 5.651 ± 0.715
0.387LysCys: 0.387 ± 0.185
3.251LysAsp: 3.251 ± 0.526
4.335LysGlu: 4.335 ± 0.626
1.161LysPhe: 1.161 ± 0.264
3.561LysGly: 3.561 ± 0.428
1.006LysHis: 1.006 ± 0.316
3.406LysIle: 3.406 ± 0.588
4.18LysLys: 4.18 ± 0.543
4.722LysLeu: 4.722 ± 0.616
1.471LysMet: 1.471 ± 0.396
1.626LysAsn: 1.626 ± 0.339
2.632LysPro: 2.632 ± 0.431
2.787LysGln: 2.787 ± 0.424
3.716LysArg: 3.716 ± 0.587
2.013LysSer: 2.013 ± 0.44
3.174LysThr: 3.174 ± 0.461
4.18LysVal: 4.18 ± 0.547
0.619LysTrp: 0.619 ± 0.23
1.161LysTyr: 1.161 ± 0.452
0.0LysXaa: 0.0 ± 0.0
Leu
9.289LeuAla: 9.289 ± 0.902
0.929LeuCys: 0.929 ± 0.282
5.496LeuAsp: 5.496 ± 0.525
4.877LeuGlu: 4.877 ± 0.577
2.013LeuPhe: 2.013 ± 0.388
5.419LeuGly: 5.419 ± 0.763
1.161LeuHis: 1.161 ± 0.299
4.258LeuIle: 4.258 ± 0.532
4.103LeuLys: 4.103 ± 0.529
5.574LeuLeu: 5.574 ± 0.85
2.632LeuMet: 2.632 ± 0.435
3.638LeuAsn: 3.638 ± 0.521
3.948LeuPro: 3.948 ± 0.421
2.864LeuGln: 2.864 ± 0.71
4.335LeuArg: 4.335 ± 0.613
4.8LeuSer: 4.8 ± 0.675
4.722LeuThr: 4.722 ± 0.538
5.496LeuVal: 5.496 ± 0.563
0.774LeuTrp: 0.774 ± 0.229
1.935LeuTyr: 1.935 ± 0.379
0.0LeuXaa: 0.0 ± 0.0
Met
4.258MetAla: 4.258 ± 0.448
0.232MetCys: 0.232 ± 0.13
1.393MetAsp: 1.393 ± 0.589
1.935MetGlu: 1.935 ± 0.372
1.161MetPhe: 1.161 ± 0.358
1.548MetGly: 1.548 ± 0.354
0.155MetHis: 0.155 ± 0.114
2.013MetIle: 2.013 ± 0.348
1.935MetLys: 1.935 ± 0.405
2.09MetLeu: 2.09 ± 0.365
0.697MetMet: 0.697 ± 0.209
1.006MetAsn: 1.006 ± 0.248
1.548MetPro: 1.548 ± 0.395
1.471MetGln: 1.471 ± 0.293
1.703MetArg: 1.703 ± 0.345
1.393MetSer: 1.393 ± 0.266
1.471MetThr: 1.471 ± 0.329
1.006MetVal: 1.006 ± 0.281
0.619MetTrp: 0.619 ± 0.21
0.697MetTyr: 0.697 ± 0.236
0.0MetXaa: 0.0 ± 0.0
Asn
4.412AsnAla: 4.412 ± 0.693
0.31AsnCys: 0.31 ± 0.14
2.942AsnAsp: 2.942 ± 0.387
2.013AsnGlu: 2.013 ± 0.322
1.084AsnPhe: 1.084 ± 0.344
4.258AsnGly: 4.258 ± 0.84
0.464AsnHis: 0.464 ± 0.185
2.555AsnIle: 2.555 ± 0.638
1.935AsnLys: 1.935 ± 0.331
2.632AsnLeu: 2.632 ± 0.368
1.161AsnMet: 1.161 ± 0.288
1.316AsnAsn: 1.316 ± 0.351
2.632AsnPro: 2.632 ± 0.496
2.322AsnGln: 2.322 ± 0.578
1.78AsnArg: 1.78 ± 0.393
2.013AsnSer: 2.013 ± 0.347
2.4AsnThr: 2.4 ± 0.457
2.632AsnVal: 2.632 ± 0.743
0.619AsnTrp: 0.619 ± 0.219
0.929AsnTyr: 0.929 ± 0.254
0.0AsnXaa: 0.0 ± 0.0
Pro
4.954ProAla: 4.954 ± 0.61
0.542ProCys: 0.542 ± 0.219
5.032ProAsp: 5.032 ± 0.623
4.877ProGlu: 4.877 ± 0.651
1.703ProPhe: 1.703 ± 0.283
3.793ProGly: 3.793 ± 0.686
0.619ProHis: 0.619 ± 0.225
2.168ProIle: 2.168 ± 0.362
1.935ProLys: 1.935 ± 0.372
3.484ProLeu: 3.484 ± 0.575
0.697ProMet: 0.697 ± 0.228
1.626ProAsn: 1.626 ± 0.276
3.096ProPro: 3.096 ± 0.534
2.245ProGln: 2.245 ± 0.441
2.245ProArg: 2.245 ± 0.408
2.322ProSer: 2.322 ± 0.373
3.174ProThr: 3.174 ± 0.474
2.787ProVal: 2.787 ± 0.405
0.929ProTrp: 0.929 ± 0.352
1.626ProTyr: 1.626 ± 0.464
0.0ProXaa: 0.0 ± 0.0
Gln
6.503GlnAla: 6.503 ± 1.243
0.542GlnCys: 0.542 ± 0.305
2.632GlnAsp: 2.632 ± 0.481
2.09GlnGlu: 2.09 ± 0.416
1.239GlnPhe: 1.239 ± 0.414
2.4GlnGly: 2.4 ± 0.499
0.774GlnHis: 0.774 ± 0.268
2.477GlnIle: 2.477 ± 0.399
3.174GlnLys: 3.174 ± 0.462
3.561GlnLeu: 3.561 ± 0.697
1.548GlnMet: 1.548 ± 0.344
1.858GlnAsn: 1.858 ± 0.388
2.787GlnPro: 2.787 ± 0.487
4.8GlnGln: 4.8 ± 1.173
2.555GlnArg: 2.555 ± 0.429
2.168GlnSer: 2.168 ± 0.456
2.322GlnThr: 2.322 ± 0.518
2.322GlnVal: 2.322 ± 0.411
0.852GlnTrp: 0.852 ± 0.21
1.084GlnTyr: 1.084 ± 0.32
0.0GlnXaa: 0.0 ± 0.0
Arg
6.115ArgAla: 6.115 ± 0.741
0.852ArgCys: 0.852 ± 0.368
4.103ArgAsp: 4.103 ± 0.485
4.335ArgGlu: 4.335 ± 0.604
2.555ArgPhe: 2.555 ± 0.432
4.258ArgGly: 4.258 ± 0.595
1.393ArgHis: 1.393 ± 0.284
2.245ArgIle: 2.245 ± 0.426
4.335ArgLys: 4.335 ± 0.65
4.567ArgLeu: 4.567 ± 0.603
1.78ArgMet: 1.78 ± 0.382
2.709ArgAsn: 2.709 ± 0.666
2.632ArgPro: 2.632 ± 0.456
2.477ArgGln: 2.477 ± 0.391
3.716ArgArg: 3.716 ± 0.496
3.251ArgSer: 3.251 ± 0.512
3.174ArgThr: 3.174 ± 0.467
3.716ArgVal: 3.716 ± 0.667
1.161ArgTrp: 1.161 ± 0.273
2.245ArgTyr: 2.245 ± 0.397
0.0ArgXaa: 0.0 ± 0.0
Ser
5.187SerAla: 5.187 ± 0.661
0.464SerCys: 0.464 ± 0.163
3.019SerAsp: 3.019 ± 0.409
2.864SerGlu: 2.864 ± 0.461
1.548SerPhe: 1.548 ± 0.401
5.419SerGly: 5.419 ± 0.777
1.393SerHis: 1.393 ± 0.379
2.168SerIle: 2.168 ± 0.37
2.4SerLys: 2.4 ± 0.371
4.258SerLeu: 4.258 ± 0.453
1.471SerMet: 1.471 ± 0.359
2.168SerAsn: 2.168 ± 0.424
2.322SerPro: 2.322 ± 0.412
2.477SerGln: 2.477 ± 0.577
2.864SerArg: 2.864 ± 0.513
2.709SerSer: 2.709 ± 0.883
2.555SerThr: 2.555 ± 0.439
3.484SerVal: 3.484 ± 0.477
0.31SerTrp: 0.31 ± 0.173
1.316SerTyr: 1.316 ± 0.367
0.0SerXaa: 0.0 ± 0.0
Thr
4.567ThrAla: 4.567 ± 0.497
0.697ThrCys: 0.697 ± 0.234
3.638ThrAsp: 3.638 ± 0.648
2.864ThrGlu: 2.864 ± 0.447
1.084ThrPhe: 1.084 ± 0.26
4.18ThrGly: 4.18 ± 0.58
0.542ThrHis: 0.542 ± 0.218
3.096ThrIle: 3.096 ± 0.495
2.632ThrLys: 2.632 ± 0.463
5.806ThrLeu: 5.806 ± 0.597
1.935ThrMet: 1.935 ± 0.392
2.632ThrAsn: 2.632 ± 0.516
3.948ThrPro: 3.948 ± 0.634
1.935ThrGln: 1.935 ± 0.476
2.709ThrArg: 2.709 ± 0.449
2.864ThrSer: 2.864 ± 0.554
3.561ThrThr: 3.561 ± 0.595
2.942ThrVal: 2.942 ± 0.497
1.006ThrTrp: 1.006 ± 0.276
1.626ThrTyr: 1.626 ± 0.25
0.0ThrXaa: 0.0 ± 0.0
Val
7.664ValAla: 7.664 ± 0.799
0.619ValCys: 0.619 ± 0.213
2.864ValAsp: 2.864 ± 0.471
4.335ValGlu: 4.335 ± 0.607
1.548ValPhe: 1.548 ± 0.377
4.258ValGly: 4.258 ± 0.551
1.548ValHis: 1.548 ± 0.312
2.942ValIle: 2.942 ± 0.466
4.025ValLys: 4.025 ± 0.607
4.103ValLeu: 4.103 ± 0.536
2.245ValMet: 2.245 ± 0.522
3.484ValAsn: 3.484 ± 0.81
3.174ValPro: 3.174 ± 0.597
2.245ValGln: 2.245 ± 0.461
3.251ValArg: 3.251 ± 0.457
3.019ValSer: 3.019 ± 0.538
4.258ValThr: 4.258 ± 0.623
4.645ValVal: 4.645 ± 0.6
0.852ValTrp: 0.852 ± 0.234
1.78ValTyr: 1.78 ± 0.417
0.0ValXaa: 0.0 ± 0.0
Trp
0.852TrpAla: 0.852 ± 0.258
0.464TrpCys: 0.464 ± 0.216
1.471TrpAsp: 1.471 ± 0.31
0.852TrpGlu: 0.852 ± 0.21
0.542TrpPhe: 0.542 ± 0.26
1.316TrpGly: 1.316 ± 0.352
0.619TrpHis: 0.619 ± 0.22
0.852TrpIle: 0.852 ± 0.318
0.542TrpLys: 0.542 ± 0.193
1.316TrpLeu: 1.316 ± 0.285
0.697TrpMet: 0.697 ± 0.241
0.929TrpAsn: 0.929 ± 0.251
0.542TrpPro: 0.542 ± 0.172
0.929TrpGln: 0.929 ± 0.312
1.161TrpArg: 1.161 ± 0.268
0.464TrpSer: 0.464 ± 0.196
1.393TrpThr: 1.393 ± 0.283
0.542TrpVal: 0.542 ± 0.185
0.155TrpTrp: 0.155 ± 0.106
0.464TrpTyr: 0.464 ± 0.146
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.4TyrAla: 2.4 ± 0.502
0.852TyrCys: 0.852 ± 0.282
2.09TyrAsp: 2.09 ± 0.392
1.316TyrGlu: 1.316 ± 0.318
1.006TyrPhe: 1.006 ± 0.238
2.322TyrGly: 2.322 ± 0.415
0.852TyrHis: 0.852 ± 0.259
1.006TyrIle: 1.006 ± 0.234
1.239TyrLys: 1.239 ± 0.38
2.245TyrLeu: 2.245 ± 0.344
0.697TyrMet: 0.697 ± 0.271
1.161TyrAsn: 1.161 ± 0.371
1.161TyrPro: 1.161 ± 0.412
0.929TyrGln: 0.929 ± 0.237
2.168TyrArg: 2.168 ± 0.335
2.09TyrSer: 2.09 ± 0.398
2.09TyrThr: 2.09 ± 0.405
2.245TyrVal: 2.245 ± 0.398
0.464TyrTrp: 0.464 ± 0.226
0.852TyrTyr: 0.852 ± 0.274
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (12919 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski