Maize yellow striate virus
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D1GTQ4|A0A2D1GTQ4_9RHAB N protein OS=Maize yellow striate virus OX=1168550 GN=N PE=4 SV=1
MM1 pKa = 7.65 ASSSDD6 pKa = 3.51 VQFLSGIPDD15 pKa = 4.94 DD16 pKa = 3.75 IHH18 pKa = 6.42 PRR20 pKa = 11.84 SYY22 pKa = 11.28 DD23 pKa = 3.39 LSADD27 pKa = 4.05 GIFSNAEE34 pKa = 3.64 EE35 pKa = 4.34 EE36 pKa = 4.36 EE37 pKa = 4.38 VMEE40 pKa = 4.31 EE41 pKa = 3.97 RR42 pKa = 11.84 QAEE45 pKa = 4.0 QDD47 pKa = 3.52 LPDD50 pKa = 4.5 IEE52 pKa = 4.88 EE53 pKa = 4.31 EE54 pKa = 4.22 EE55 pKa = 4.29 PLVEE59 pKa = 5.18 KK60 pKa = 9.77 PAKK63 pKa = 10.07 GEE65 pKa = 4.1 KK66 pKa = 9.59 FVSGTDD72 pKa = 3.45 EE73 pKa = 4.18 LVSILKK79 pKa = 10.31 DD80 pKa = 3.3 YY81 pKa = 11.34 SNRR84 pKa = 11.84 KK85 pKa = 8.06 GMGMKK90 pKa = 10.13 KK91 pKa = 9.4 EE92 pKa = 4.06 WVNIMKK98 pKa = 10.28 RR99 pKa = 11.84 RR100 pKa = 11.84 FHH102 pKa = 6.65 EE103 pKa = 4.15 MGGKK107 pKa = 7.9 MLKK110 pKa = 8.1 THH112 pKa = 6.44 VDD114 pKa = 4.21 FFLLGIQAEE123 pKa = 4.55 RR124 pKa = 11.84 NMSVDD129 pKa = 3.9 RR130 pKa = 11.84 DD131 pKa = 4.06 FKK133 pKa = 10.98 DD134 pKa = 2.87 TATRR138 pKa = 11.84 LSDD141 pKa = 3.23 EE142 pKa = 4.45 VNRR145 pKa = 11.84 VSGVGKK151 pKa = 10.61 RR152 pKa = 11.84 MLDD155 pKa = 3.23 AQQKK159 pKa = 7.36 MEE161 pKa = 4.68 RR162 pKa = 11.84 EE163 pKa = 4.3 MEE165 pKa = 4.13 QKK167 pKa = 9.57 MKK169 pKa = 10.57 EE170 pKa = 3.82 ITAHH174 pKa = 5.34 CKK176 pKa = 9.73 KK177 pKa = 9.87 MEE179 pKa = 4.01 MMVAKK184 pKa = 10.77 VEE186 pKa = 4.33 TAVEE190 pKa = 4.08 NASRR194 pKa = 11.84 PSSISSWAMGGPSRR208 pKa = 11.84 EE209 pKa = 4.09 EE210 pKa = 3.86 EE211 pKa = 4.04 EE212 pKa = 4.0 EE213 pKa = 3.83 RR214 pKa = 11.84 DD215 pKa = 3.48 YY216 pKa = 11.87 DD217 pKa = 4.48 KK218 pKa = 10.87 FLSMIGFEE226 pKa = 4.53 DD227 pKa = 3.54 KK228 pKa = 10.58 HH229 pKa = 7.47 IKK231 pKa = 8.91 SATMKK236 pKa = 9.74 KK237 pKa = 9.66 CYY239 pKa = 9.24 PAISDD244 pKa = 3.27 EE245 pKa = 4.57 MYY247 pKa = 9.56 VHH249 pKa = 7.0 VITGEE254 pKa = 3.88 ADD256 pKa = 3.13 HH257 pKa = 8.04 ADD259 pKa = 3.16 MTNYY263 pKa = 9.71 YY264 pKa = 9.8 RR265 pKa = 11.84 LIMEE269 pKa = 4.22 YY270 pKa = 10.94 AEE272 pKa = 4.29 SKK274 pKa = 9.9 VLKK277 pKa = 9.21 KK278 pKa = 10.69 KK279 pKa = 10.58 SVAASRR285 pKa = 11.84 NDD287 pKa = 3.75 PYY289 pKa = 11.06 PGLL292 pKa = 3.99
Molecular weight: 33.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.089
IPC2_protein 5.08
IPC_protein 5.016
Toseland 4.94
ProMoST 5.143
Dawson 4.991
Bjellqvist 5.118
Wikipedia 4.838
Rodwell 4.914
Grimsley 4.863
Solomon 4.991
Lehninger 4.94
Nozaki 5.105
DTASelect 5.232
Thurlkill 4.94
EMBOSS 4.876
Sillero 5.181
Patrickios 4.317
IPC_peptide 5.003
IPC2_peptide 5.181
IPC2.peptide.svr19 5.163
Protein with the highest isoelectric point:
>tr|A0A2D1GTP8|A0A2D1GTP8_9RHAB P protein OS=Maize yellow striate virus OX=1168550 GN=P PE=4 SV=1
MM1 pKa = 7.7 WNFLLHH7 pKa = 5.32 VLRR10 pKa = 11.84 DD11 pKa = 3.62 GSDD14 pKa = 3.36 RR15 pKa = 11.84 TNMSSSWTVVSLSAYY30 pKa = 9.72 AILVFIISISICRR43 pKa = 11.84 LKK45 pKa = 10.79 VYY47 pKa = 7.94 QLRR50 pKa = 11.84 LIVIISLCHH59 pKa = 5.6 LVSAIGSAFKK69 pKa = 10.5 EE70 pKa = 4.34 WTT72 pKa = 3.41
Molecular weight: 8.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.812
IPC2_protein 8.521
IPC_protein 8.814
Toseland 8.916
ProMoST 8.887
Dawson 9.194
Bjellqvist 9.297
Wikipedia 9.268
Rodwell 9.224
Grimsley 8.814
Solomon 9.545
Lehninger 9.531
Nozaki 9.458
DTASelect 9.121
Thurlkill 9.165
EMBOSS 9.37
Sillero 9.414
Patrickios 4.673
IPC_peptide 9.531
IPC2_peptide 8.624
IPC2.peptide.svr19 7.857
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
3954
51
2056
395.4
44.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.982 ± 0.645
1.669 ± 0.477
5.387 ± 0.42
6.5 ± 0.703
3.92 ± 0.249
5.589 ± 0.359
2.15 ± 0.313
7.031 ± 0.49
6.651 ± 0.529
9.611 ± 1.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.288 ± 0.497
4.401 ± 0.367
4.299 ± 0.44
2.731 ± 0.314
5.438 ± 0.327
8.574 ± 0.596
5.918 ± 0.694
6.348 ± 0.451
1.593 ± 0.285
3.92 ± 0.422
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here