Klebsiella phage phiKO2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6UAU3|Q6UAU3_9CAUD Gp39 OS=Klebsiella phage phiKO2 OX=255431 GN=39 PE=4 SV=1
MM1 pKa = 7.41NSEE4 pKa = 4.38LFNYY8 pKa = 9.32SLKK11 pKa = 10.01TLLVLNFIADD21 pKa = 4.12LVLFAVLFGFGYY33 pKa = 11.03GLLSLLEE40 pKa = 3.82WWLII44 pKa = 3.8

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6UAT0|Q6UAT0_9CAUD Putative DNA adenine methylase OS=Klebsiella phage phiKO2 OX=255431 GN=52 PE=3 SV=1
MM1 pKa = 6.71NTAIEE6 pKa = 4.32YY7 pKa = 9.62GNPDD11 pKa = 3.47LWLVLLMLAAGVISSALLSEE31 pKa = 4.26NPINARR37 pKa = 11.84RR38 pKa = 11.84LLGDD42 pKa = 3.1VLRR45 pKa = 11.84GVIVAIIIWTYY56 pKa = 10.28GVMGNVSILKK66 pKa = 10.24VITLAGLSAVAWPHH80 pKa = 4.68TVNEE84 pKa = 3.86ITRR87 pKa = 11.84FAKK90 pKa = 9.42RR91 pKa = 11.84TISRR95 pKa = 11.84FFGGRR100 pKa = 11.84KK101 pKa = 8.91

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

16027

33

3433

250.4

27.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.546 ± 0.438

1.067 ± 0.192

5.871 ± 0.224

6.37 ± 0.573

3.032 ± 0.277

7.207 ± 0.384

1.604 ± 0.256

5.503 ± 0.183

5.422 ± 0.366

8.648 ± 0.523

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.508 ± 0.336

4.667 ± 0.475

3.731 ± 0.276

4.686 ± 0.367

5.853 ± 0.425

7.263 ± 0.751

6.676 ± 0.79

5.959 ± 0.269

1.473 ± 0.141

2.914 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski