Amino acid dipepetide frequency for Bacillus phage BtCS33

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.639AlaAla: 4.639 ± 1.031
0.843AlaCys: 0.843 ± 0.337
3.627AlaAsp: 3.627 ± 0.617
4.386AlaGlu: 4.386 ± 0.716
2.109AlaPhe: 2.109 ± 0.409
3.458AlaGly: 3.458 ± 0.801
0.675AlaHis: 0.675 ± 0.268
4.049AlaIle: 4.049 ± 0.521
6.832AlaLys: 6.832 ± 0.733
5.482AlaLeu: 5.482 ± 0.729
2.446AlaMet: 2.446 ± 0.518
3.374AlaAsn: 3.374 ± 0.72
1.603AlaPro: 1.603 ± 0.282
2.024AlaGln: 2.024 ± 0.572
3.121AlaArg: 3.121 ± 0.523
3.121AlaSer: 3.121 ± 0.607
4.217AlaThr: 4.217 ± 0.995
4.217AlaVal: 4.217 ± 0.993
0.59AlaTrp: 0.59 ± 0.204
2.109AlaTyr: 2.109 ± 0.46
0.0AlaXaa: 0.0 ± 0.0
Cys
0.422CysAla: 0.422 ± 0.169
0.084CysCys: 0.084 ± 0.078
1.265CysAsp: 1.265 ± 0.388
0.506CysGlu: 0.506 ± 0.183
0.506CysPhe: 0.506 ± 0.196
0.506CysGly: 0.506 ± 0.237
0.422CysHis: 0.422 ± 0.238
0.253CysIle: 0.253 ± 0.167
0.337CysLys: 0.337 ± 0.191
0.506CysLeu: 0.506 ± 0.252
0.59CysMet: 0.59 ± 0.215
0.422CysAsn: 0.422 ± 0.257
0.337CysPro: 0.337 ± 0.175
0.337CysGln: 0.337 ± 0.194
0.506CysArg: 0.506 ± 0.206
0.506CysSer: 0.506 ± 0.218
0.506CysThr: 0.506 ± 0.188
0.506CysVal: 0.506 ± 0.193
0.0CysTrp: 0.0 ± 0.0
0.337CysTyr: 0.337 ± 0.175
0.0CysXaa: 0.0 ± 0.0
Asp
3.374AspAla: 3.374 ± 0.674
0.253AspCys: 0.253 ± 0.136
2.868AspAsp: 2.868 ± 0.518
5.061AspGlu: 5.061 ± 0.632
2.446AspPhe: 2.446 ± 0.437
3.458AspGly: 3.458 ± 0.406
0.843AspHis: 0.843 ± 0.232
4.47AspIle: 4.47 ± 0.604
4.976AspLys: 4.976 ± 0.491
5.314AspLeu: 5.314 ± 0.661
2.277AspMet: 2.277 ± 0.534
2.362AspAsn: 2.362 ± 0.465
1.434AspPro: 1.434 ± 0.442
1.687AspGln: 1.687 ± 0.393
2.277AspArg: 2.277 ± 0.381
2.53AspSer: 2.53 ± 0.346
2.699AspThr: 2.699 ± 0.394
3.543AspVal: 3.543 ± 0.471
0.759AspTrp: 0.759 ± 0.201
2.615AspTyr: 2.615 ± 0.425
0.0AspXaa: 0.0 ± 0.0
Glu
5.651GluAla: 5.651 ± 0.643
0.843GluCys: 0.843 ± 0.267
3.627GluAsp: 3.627 ± 0.597
7.085GluGlu: 7.085 ± 0.731
4.302GluPhe: 4.302 ± 0.639
4.049GluGly: 4.049 ± 0.587
1.94GluHis: 1.94 ± 0.388
7.507GluIle: 7.507 ± 0.652
8.688GluLys: 8.688 ± 0.769
7.507GluLeu: 7.507 ± 0.802
2.783GluMet: 2.783 ± 0.489
4.302GluAsn: 4.302 ± 0.562
1.771GluPro: 1.771 ± 0.552
4.386GluGln: 4.386 ± 0.623
3.964GluArg: 3.964 ± 0.697
4.049GluSer: 4.049 ± 0.628
3.374GluThr: 3.374 ± 0.546
4.723GluVal: 4.723 ± 0.665
0.843GluTrp: 0.843 ± 0.263
2.446GluTyr: 2.446 ± 0.56
0.0GluXaa: 0.0 ± 0.0
Phe
2.446PheAla: 2.446 ± 0.373
0.337PheCys: 0.337 ± 0.165
2.193PheAsp: 2.193 ± 0.449
2.952PheGlu: 2.952 ± 0.501
1.181PhePhe: 1.181 ± 0.367
3.374PheGly: 3.374 ± 0.565
0.759PheHis: 0.759 ± 0.265
3.036PheIle: 3.036 ± 0.529
3.796PheLys: 3.796 ± 0.599
3.88PheLeu: 3.88 ± 0.596
1.434PheMet: 1.434 ± 0.354
3.036PheAsn: 3.036 ± 0.402
0.675PhePro: 0.675 ± 0.23
1.434PheGln: 1.434 ± 0.344
1.603PheArg: 1.603 ± 0.376
2.193PheSer: 2.193 ± 0.467
2.109PheThr: 2.109 ± 0.442
2.024PheVal: 2.024 ± 0.393
0.675PheTrp: 0.675 ± 0.298
1.434PheTyr: 1.434 ± 0.277
0.0PheXaa: 0.0 ± 0.0
Gly
2.783GlyAla: 2.783 ± 0.704
0.253GlyCys: 0.253 ± 0.163
2.446GlyAsp: 2.446 ± 0.423
5.145GlyGlu: 5.145 ± 0.679
2.53GlyPhe: 2.53 ± 0.565
2.53GlyGly: 2.53 ± 0.441
0.759GlyHis: 0.759 ± 0.246
3.796GlyIle: 3.796 ± 0.623
5.482GlyLys: 5.482 ± 0.76
4.555GlyLeu: 4.555 ± 0.744
2.446GlyMet: 2.446 ± 0.701
2.53GlyAsn: 2.53 ± 0.377
0.59GlyPro: 0.59 ± 0.21
1.687GlyGln: 1.687 ± 0.28
2.952GlyArg: 2.952 ± 0.569
3.289GlySer: 3.289 ± 0.529
2.783GlyThr: 2.783 ± 0.468
4.47GlyVal: 4.47 ± 0.595
1.012GlyTrp: 1.012 ± 0.304
2.446GlyTyr: 2.446 ± 0.401
0.0GlyXaa: 0.0 ± 0.0
His
0.759HisAla: 0.759 ± 0.236
0.422HisCys: 0.422 ± 0.205
0.422HisAsp: 0.422 ± 0.169
1.35HisGlu: 1.35 ± 0.26
0.675HisPhe: 0.675 ± 0.233
1.012HisGly: 1.012 ± 0.306
0.337HisHis: 0.337 ± 0.21
1.012HisIle: 1.012 ± 0.231
2.109HisLys: 2.109 ± 0.494
2.024HisLeu: 2.024 ± 0.392
0.59HisMet: 0.59 ± 0.207
1.096HisAsn: 1.096 ± 0.338
0.337HisPro: 0.337 ± 0.185
0.759HisGln: 0.759 ± 0.275
0.843HisArg: 0.843 ± 0.292
1.94HisSer: 1.94 ± 0.404
1.35HisThr: 1.35 ± 0.403
0.843HisVal: 0.843 ± 0.24
0.169HisTrp: 0.169 ± 0.112
0.59HisTyr: 0.59 ± 0.193
0.0HisXaa: 0.0 ± 0.0
Ile
5.651IleAla: 5.651 ± 0.564
0.506IleCys: 0.506 ± 0.183
3.796IleAsp: 3.796 ± 0.565
7.422IleGlu: 7.422 ± 0.793
2.024IlePhe: 2.024 ± 0.413
3.205IleGly: 3.205 ± 0.658
1.518IleHis: 1.518 ± 0.344
4.723IleIle: 4.723 ± 0.647
5.904IleLys: 5.904 ± 0.522
3.964IleLeu: 3.964 ± 0.469
2.193IleMet: 2.193 ± 0.478
5.229IleAsn: 5.229 ± 0.737
2.193IlePro: 2.193 ± 0.45
3.543IleGln: 3.543 ± 0.498
3.036IleArg: 3.036 ± 0.416
3.964IleSer: 3.964 ± 0.512
4.639IleThr: 4.639 ± 0.561
3.796IleVal: 3.796 ± 0.622
0.843IleTrp: 0.843 ± 0.272
2.277IleTyr: 2.277 ± 0.48
0.0IleXaa: 0.0 ± 0.0
Lys
6.579LysAla: 6.579 ± 0.74
0.843LysCys: 0.843 ± 0.26
4.386LysAsp: 4.386 ± 0.575
8.182LysGlu: 8.182 ± 0.734
3.627LysPhe: 3.627 ± 0.41
3.374LysGly: 3.374 ± 0.601
1.94LysHis: 1.94 ± 0.421
6.157LysIle: 6.157 ± 0.516
9.025LysLys: 9.025 ± 1.104
6.832LysLeu: 6.832 ± 0.835
2.699LysMet: 2.699 ± 0.521
5.482LysAsn: 5.482 ± 0.547
2.615LysPro: 2.615 ± 0.634
5.989LysGln: 5.989 ± 0.826
5.314LysArg: 5.314 ± 0.662
5.482LysSer: 5.482 ± 0.494
5.989LysThr: 5.989 ± 0.584
6.157LysVal: 6.157 ± 0.685
1.181LysTrp: 1.181 ± 0.317
3.374LysTyr: 3.374 ± 0.466
0.0LysXaa: 0.0 ± 0.0
Leu
5.482LeuAla: 5.482 ± 0.867
1.181LeuCys: 1.181 ± 0.427
5.061LeuAsp: 5.061 ± 0.627
6.832LeuGlu: 6.832 ± 0.845
2.362LeuPhe: 2.362 ± 0.451
4.386LeuGly: 4.386 ± 0.69
2.024LeuHis: 2.024 ± 0.433
4.976LeuIle: 4.976 ± 0.787
8.772LeuLys: 8.772 ± 0.871
5.398LeuLeu: 5.398 ± 0.96
1.181LeuMet: 1.181 ± 0.372
5.482LeuAsn: 5.482 ± 0.829
2.615LeuPro: 2.615 ± 0.504
3.627LeuGln: 3.627 ± 0.582
4.217LeuArg: 4.217 ± 0.541
5.735LeuSer: 5.735 ± 0.741
3.543LeuThr: 3.543 ± 0.586
4.47LeuVal: 4.47 ± 0.534
0.759LeuTrp: 0.759 ± 0.254
2.783LeuTyr: 2.783 ± 0.541
0.0LeuXaa: 0.0 ± 0.0
Met
1.434MetAla: 1.434 ± 0.317
0.169MetCys: 0.169 ± 0.117
1.94MetAsp: 1.94 ± 0.434
2.868MetGlu: 2.868 ± 0.391
0.928MetPhe: 0.928 ± 0.363
1.94MetGly: 1.94 ± 0.509
0.169MetHis: 0.169 ± 0.13
1.687MetIle: 1.687 ± 0.336
4.133MetLys: 4.133 ± 0.576
2.868MetLeu: 2.868 ± 0.513
0.843MetMet: 0.843 ± 0.277
1.771MetAsn: 1.771 ± 0.367
0.759MetPro: 0.759 ± 0.215
1.434MetGln: 1.434 ± 0.387
1.434MetArg: 1.434 ± 0.345
1.265MetSer: 1.265 ± 0.273
1.94MetThr: 1.94 ± 0.39
1.35MetVal: 1.35 ± 0.301
0.422MetTrp: 0.422 ± 0.167
0.759MetTyr: 0.759 ± 0.308
0.0MetXaa: 0.0 ± 0.0
Asn
3.711AsnAla: 3.711 ± 0.658
0.337AsnCys: 0.337 ± 0.167
2.868AsnAsp: 2.868 ± 0.545
5.229AsnGlu: 5.229 ± 0.599
1.856AsnPhe: 1.856 ± 0.459
3.711AsnGly: 3.711 ± 0.567
1.265AsnHis: 1.265 ± 0.304
4.302AsnIle: 4.302 ± 0.575
5.145AsnLys: 5.145 ± 0.635
4.386AsnLeu: 4.386 ± 0.68
1.771AsnMet: 1.771 ± 0.313
2.615AsnAsn: 2.615 ± 0.302
2.024AsnPro: 2.024 ± 0.491
2.868AsnGln: 2.868 ± 0.41
2.868AsnArg: 2.868 ± 0.489
3.205AsnSer: 3.205 ± 0.513
3.543AsnThr: 3.543 ± 0.522
3.036AsnVal: 3.036 ± 0.397
0.759AsnTrp: 0.759 ± 0.274
2.109AsnTyr: 2.109 ± 0.487
0.0AsnXaa: 0.0 ± 0.0
Pro
0.928ProAla: 0.928 ± 0.226
0.337ProCys: 0.337 ± 0.17
1.518ProAsp: 1.518 ± 0.419
1.94ProGlu: 1.94 ± 0.561
1.012ProPhe: 1.012 ± 0.275
1.181ProGly: 1.181 ± 0.376
0.843ProHis: 0.843 ± 0.181
2.615ProIle: 2.615 ± 0.516
1.94ProLys: 1.94 ± 0.509
2.024ProLeu: 2.024 ± 0.411
0.337ProMet: 0.337 ± 0.148
2.193ProAsn: 2.193 ± 0.46
1.012ProPro: 1.012 ± 0.345
0.928ProGln: 0.928 ± 0.391
0.59ProArg: 0.59 ± 0.208
2.193ProSer: 2.193 ± 0.381
1.94ProThr: 1.94 ± 0.508
1.434ProVal: 1.434 ± 0.589
0.422ProTrp: 0.422 ± 0.17
1.603ProTyr: 1.603 ± 0.384
0.0ProXaa: 0.0 ± 0.0
Gln
3.289GlnAla: 3.289 ± 0.848
0.253GlnCys: 0.253 ± 0.126
1.94GlnAsp: 1.94 ± 0.37
3.289GlnGlu: 3.289 ± 0.686
1.687GlnPhe: 1.687 ± 0.475
2.446GlnGly: 2.446 ± 0.4
0.759GlnHis: 0.759 ± 0.431
3.121GlnIle: 3.121 ± 0.553
5.567GlnLys: 5.567 ± 0.884
3.036GlnLeu: 3.036 ± 0.565
1.518GlnMet: 1.518 ± 0.328
2.446GlnAsn: 2.446 ± 0.511
1.35GlnPro: 1.35 ± 0.281
2.952GlnGln: 2.952 ± 0.493
2.109GlnArg: 2.109 ± 0.367
2.446GlnSer: 2.446 ± 0.443
2.277GlnThr: 2.277 ± 0.425
2.109GlnVal: 2.109 ± 0.425
0.506GlnTrp: 0.506 ± 0.208
2.024GlnTyr: 2.024 ± 0.366
0.0GlnXaa: 0.0 ± 0.0
Arg
2.446ArgAla: 2.446 ± 0.512
0.253ArgCys: 0.253 ± 0.151
3.796ArgAsp: 3.796 ± 0.477
3.796ArgGlu: 3.796 ± 0.601
2.277ArgPhe: 2.277 ± 0.357
3.289ArgGly: 3.289 ± 0.502
0.59ArgHis: 0.59 ± 0.206
3.121ArgIle: 3.121 ± 0.558
3.964ArgLys: 3.964 ± 0.464
4.555ArgLeu: 4.555 ± 0.679
1.35ArgMet: 1.35 ± 0.309
1.856ArgAsn: 1.856 ± 0.367
1.35ArgPro: 1.35 ± 0.299
2.53ArgGln: 2.53 ± 0.58
1.94ArgArg: 1.94 ± 0.354
1.518ArgSer: 1.518 ± 0.489
2.024ArgThr: 2.024 ± 0.545
3.121ArgVal: 3.121 ± 0.521
0.506ArgTrp: 0.506 ± 0.18
1.687ArgTyr: 1.687 ± 0.382
0.0ArgXaa: 0.0 ± 0.0
Ser
3.205SerAla: 3.205 ± 0.682
0.337SerCys: 0.337 ± 0.167
3.88SerAsp: 3.88 ± 0.386
4.217SerGlu: 4.217 ± 0.632
3.036SerPhe: 3.036 ± 0.632
3.289SerGly: 3.289 ± 0.504
1.181SerHis: 1.181 ± 0.389
4.976SerIle: 4.976 ± 0.706
4.386SerLys: 4.386 ± 0.598
4.049SerLeu: 4.049 ± 0.522
1.603SerMet: 1.603 ± 0.391
3.627SerAsn: 3.627 ± 0.589
1.856SerPro: 1.856 ± 0.417
1.856SerGln: 1.856 ± 0.387
2.53SerArg: 2.53 ± 0.447
3.205SerSer: 3.205 ± 0.48
2.952SerThr: 2.952 ± 0.547
3.964SerVal: 3.964 ± 0.72
0.253SerTrp: 0.253 ± 0.159
2.446SerTyr: 2.446 ± 0.344
0.0SerXaa: 0.0 ± 0.0
Thr
3.121ThrAla: 3.121 ± 0.584
0.253ThrCys: 0.253 ± 0.145
2.783ThrAsp: 2.783 ± 0.42
4.808ThrGlu: 4.808 ± 0.718
2.53ThrPhe: 2.53 ± 0.418
3.205ThrGly: 3.205 ± 0.602
0.759ThrHis: 0.759 ± 0.201
3.205ThrIle: 3.205 ± 0.62
6.157ThrLys: 6.157 ± 0.779
5.651ThrLeu: 5.651 ± 0.766
0.928ThrMet: 0.928 ± 0.285
3.036ThrAsn: 3.036 ± 0.809
1.518ThrPro: 1.518 ± 0.473
1.518ThrGln: 1.518 ± 0.319
1.856ThrArg: 1.856 ± 0.386
3.543ThrSer: 3.543 ± 0.54
4.386ThrThr: 4.386 ± 0.703
4.049ThrVal: 4.049 ± 0.439
0.675ThrTrp: 0.675 ± 0.185
1.856ThrTyr: 1.856 ± 0.393
0.0ThrXaa: 0.0 ± 0.0
Val
4.133ValAla: 4.133 ± 0.628
0.59ValCys: 0.59 ± 0.243
4.47ValAsp: 4.47 ± 0.595
5.061ValGlu: 5.061 ± 0.885
2.53ValPhe: 2.53 ± 0.457
3.88ValGly: 3.88 ± 0.662
0.675ValHis: 0.675 ± 0.21
3.796ValIle: 3.796 ± 0.53
4.302ValLys: 4.302 ± 0.607
4.217ValLeu: 4.217 ± 0.563
1.687ValMet: 1.687 ± 0.336
3.543ValAsn: 3.543 ± 0.474
1.94ValPro: 1.94 ± 0.355
3.121ValGln: 3.121 ± 0.466
2.109ValArg: 2.109 ± 0.444
4.555ValSer: 4.555 ± 0.512
3.121ValThr: 3.121 ± 0.516
3.964ValVal: 3.964 ± 0.617
0.928ValTrp: 0.928 ± 0.326
2.783ValTyr: 2.783 ± 0.628
0.0ValXaa: 0.0 ± 0.0
Trp
0.59TrpAla: 0.59 ± 0.208
0.169TrpCys: 0.169 ± 0.106
0.843TrpAsp: 0.843 ± 0.225
1.012TrpGlu: 1.012 ± 0.282
0.59TrpPhe: 0.59 ± 0.26
0.253TrpGly: 0.253 ± 0.191
0.169TrpHis: 0.169 ± 0.107
0.759TrpIle: 0.759 ± 0.267
1.012TrpLys: 1.012 ± 0.292
1.012TrpLeu: 1.012 ± 0.307
0.337TrpMet: 0.337 ± 0.185
0.928TrpAsn: 0.928 ± 0.296
0.084TrpPro: 0.084 ± 0.089
0.843TrpGln: 0.843 ± 0.265
0.675TrpArg: 0.675 ± 0.263
0.675TrpSer: 0.675 ± 0.215
0.506TrpThr: 0.506 ± 0.206
1.012TrpVal: 1.012 ± 0.369
0.084TrpTrp: 0.084 ± 0.081
0.337TrpTyr: 0.337 ± 0.128
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.277TyrAla: 2.277 ± 0.517
0.59TyrCys: 0.59 ± 0.208
1.518TyrAsp: 1.518 ± 0.412
2.699TyrGlu: 2.699 ± 0.405
2.362TyrPhe: 2.362 ± 0.422
1.94TyrGly: 1.94 ± 0.389
1.012TyrHis: 1.012 ± 0.271
2.868TyrIle: 2.868 ± 0.507
2.783TyrLys: 2.783 ± 0.548
3.627TyrLeu: 3.627 ± 0.832
1.012TyrMet: 1.012 ± 0.297
2.277TyrAsn: 2.277 ± 0.414
0.928TyrPro: 0.928 ± 0.341
1.603TyrGln: 1.603 ± 0.271
2.024TyrArg: 2.024 ± 0.409
1.434TyrSer: 1.434 ± 0.367
1.94TyrThr: 1.94 ± 0.485
2.615TyrVal: 2.615 ± 0.476
0.506TyrTrp: 0.506 ± 0.192
1.096TyrTyr: 1.096 ± 0.377
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (11857 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski