Clostridium sp. CAG:226

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2034 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5B674|R5B674_9CLOT GDSL-like protein OS=Clostridium sp. CAG:226 OX=1262781 GN=BN545_01138 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.37KK3 pKa = 9.65IFAFVLSIIMAAACMAACSPSNSAPASDD31 pKa = 4.88ADD33 pKa = 3.71GKK35 pKa = 7.15TVKK38 pKa = 10.61VGICNYY44 pKa = 10.14VDD46 pKa = 3.86DD47 pKa = 5.74ASLNQIVEE55 pKa = 4.51NIQARR60 pKa = 11.84LKK62 pKa = 10.75EE63 pKa = 4.11IGDD66 pKa = 3.87EE67 pKa = 4.06KK68 pKa = 11.22GVAFDD73 pKa = 4.9VSYY76 pKa = 11.39DD77 pKa = 3.52NCNADD82 pKa = 4.86ANLLTQIISNFIADD96 pKa = 4.96DD97 pKa = 3.78VDD99 pKa = 3.73IMIGVATPVAMAMQAATEE117 pKa = 4.31DD118 pKa = 3.19NGIPVVFAAVSDD130 pKa = 3.99PVGAGLVEE138 pKa = 4.57SMEE141 pKa = 4.25APGRR145 pKa = 11.84NVTGTSDD152 pKa = 3.91YY153 pKa = 11.14LDD155 pKa = 3.78TAAVMQLITAADD167 pKa = 3.83PDD169 pKa = 3.99AKK171 pKa = 10.81NIALLYY177 pKa = 10.65DD178 pKa = 3.77IGQDD182 pKa = 3.1SSTAAIAHH190 pKa = 5.85AKK192 pKa = 9.95EE193 pKa = 3.89YY194 pKa = 11.09LDD196 pKa = 4.42ANGIAYY202 pKa = 9.85GEE204 pKa = 4.27YY205 pKa = 9.75TGTTVDD211 pKa = 3.7EE212 pKa = 4.31VTLAVQALIADD223 pKa = 4.05GVDD226 pKa = 3.35AVFTPTDD233 pKa = 3.43NTIMKK238 pKa = 9.58SEE240 pKa = 3.88LSIYY244 pKa = 10.89EE245 pKa = 4.08MLQEE249 pKa = 4.65AGIPHH254 pKa = 5.56YY255 pKa = 9.92TGADD259 pKa = 3.18SFALNGAFLGYY270 pKa = 10.39GVDD273 pKa = 3.89YY274 pKa = 10.86AALGVEE280 pKa = 4.55TANMAASIALGGAKK294 pKa = 9.69PAEE297 pKa = 4.3TAVKK301 pKa = 9.81TFDD304 pKa = 3.93NGCATINTEE313 pKa = 3.98TCEE316 pKa = 4.07KK317 pKa = 10.87LGFNYY322 pKa = 10.65DD323 pKa = 4.27EE324 pKa = 4.21ICKK327 pKa = 10.29AFEE330 pKa = 4.12PFCTKK335 pKa = 9.77IQSIVTAEE343 pKa = 4.07SFDD346 pKa = 3.77EE347 pKa = 4.67VKK349 pKa = 10.9

Molecular weight: 36.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5BD21|R5BD21_9CLOT Isoleucine--tRNA ligase OS=Clostridium sp. CAG:226 OX=1262781 GN=ileS PE=3 SV=1
MM1 pKa = 7.7LGTFQPKK8 pKa = 9.08KK9 pKa = 8.1RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 7.68KK14 pKa = 8.56VHH16 pKa = 6.03GFRR19 pKa = 11.84QRR21 pKa = 11.84MKK23 pKa = 9.67TRR25 pKa = 11.84NGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NKK37 pKa = 9.2GRR39 pKa = 11.84KK40 pKa = 8.62VLSAA44 pKa = 4.05

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2034

0

2034

606381

29

1420

298.1

32.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.231 ± 0.077

1.673 ± 0.025

5.626 ± 0.049

6.836 ± 0.057

3.696 ± 0.036

7.814 ± 0.046

1.719 ± 0.028

6.941 ± 0.04

5.471 ± 0.042

8.913 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.354 ± 0.025

3.875 ± 0.034

3.688 ± 0.032

2.64 ± 0.034

5.312 ± 0.057

5.619 ± 0.041

5.35 ± 0.044

6.688 ± 0.048

0.805 ± 0.017

3.748 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski