Ancylostoma duodenale
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 27403 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C2G3Q3|A0A0C2G3Q3_9BILA Uncharacterized protein OS=Ancylostoma duodenale OX=51022 GN=ANCDUO_14257 PE=3 SV=1
MM1 pKa = 7.74 EE2 pKa = 5.93 DD3 pKa = 3.5 ADD5 pKa = 4.11 GMFAEE10 pKa = 5.31 GVEE13 pKa = 4.16 QVFDD17 pKa = 4.03 CSEE20 pKa = 4.07 LTKK23 pKa = 11.09 SGDD26 pKa = 3.54 DD27 pKa = 4.13 DD28 pKa = 4.02 QFSDD32 pKa = 5.27 DD33 pKa = 6.46 DD34 pKa = 4.53 EE35 pKa = 5.17 DD36 pKa = 3.8 QQSTSNVSDD45 pKa = 3.63 AGTQEE50 pKa = 4.08 VVDD53 pKa = 5.0 DD54 pKa = 4.59 SSCALQAHH62 pKa = 5.76 EE63 pKa = 4.6 QDD65 pKa = 4.1 CFSIAVAAEE74 pKa = 3.74 RR75 pKa = 11.84 WLASGGEE82 pKa = 4.06 DD83 pKa = 3.93 DD84 pKa = 6.05 VAYY87 pKa = 10.4 LWDD90 pKa = 4.03 HH91 pKa = 5.74 QVSDD95 pKa = 4.2 SDD97 pKa = 3.81 PVLKK101 pKa = 10.42 VDD103 pKa = 4.06 HH104 pKa = 6.65 QDD106 pKa = 3.05 SVTTVAFNNSQTLLSTGDD124 pKa = 3.44 MSGHH128 pKa = 5.64 IVVTQLCDD136 pKa = 3.65 LNVRR140 pKa = 11.84 AKK142 pKa = 10.25 IDD144 pKa = 3.76 DD145 pKa = 4.16 SNDD148 pKa = 3.48 LEE150 pKa = 4.49 WMCWHH155 pKa = 6.19 STSDD159 pKa = 3.5 ILFAGDD165 pKa = 3.19 KK166 pKa = 11.12 DD167 pKa = 3.93 GVVWMWLIGPAGVAQSKK184 pKa = 9.63 VCSAYY189 pKa = 10.91 SCILFIAITPNVRR202 pKa = 11.84 RR203 pKa = 11.84 IRR205 pKa = 11.84 CLLVMVHH212 pKa = 6.57 PVLLVIFFQMVNDD225 pKa = 3.75 YY226 pKa = 8.79 WQAIVMVQQ234 pKa = 3.01
Molecular weight: 25.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.579
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.63
Grimsley 3.49
Solomon 3.821
Lehninger 3.77
Nozaki 3.935
DTASelect 4.228
Thurlkill 3.643
EMBOSS 3.795
Sillero 3.935
Patrickios 1.914
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|A0A0C2G0F2|A0A0C2G0F2_9BILA Uncharacterized protein OS=Ancylostoma duodenale OX=51022 GN=ANCDUO_19355 PE=4 SV=1
RR1 pKa = 7.13 WLWRR5 pKa = 11.84 PLGIWWWTWILWTTMGRR22 pKa = 11.84 IRR24 pKa = 11.84 RR25 pKa = 11.84 LWRR28 pKa = 11.84 IPRR31 pKa = 11.84 NVWWKK36 pKa = 10.55 RR37 pKa = 11.84 IRR39 pKa = 11.84 SWSSHH44 pKa = 4.54 RR45 pKa = 11.84 NAIRR49 pKa = 11.84 MM50 pKa = 3.75
Molecular weight: 6.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.522
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.51
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.544
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.281
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.213
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
27403
0
27403
5822372
20
4472
212.5
23.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.344 ± 0.017
2.17 ± 0.014
5.438 ± 0.014
6.611 ± 0.021
4.068 ± 0.013
5.926 ± 0.024
2.413 ± 0.008
5.254 ± 0.014
5.734 ± 0.019
8.864 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.684 ± 0.009
4.082 ± 0.011
5.271 ± 0.022
3.953 ± 0.015
6.261 ± 0.016
7.411 ± 0.021
5.604 ± 0.019
6.673 ± 0.014
1.197 ± 0.007
3.042 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here