Xanthomonas phage Xoo-sp2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pamexvirus; unclassified Pamexvirus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9IAP8|A0A1X9IAP8_9CAUD Uncharacterized protein OS=Xanthomonas phage Xoo-sp2 OX=1852622 GN=Xoosp2_40 PE=4 SV=1
MM1 pKa = 7.62AAFSCNICGRR11 pKa = 11.84GFDD14 pKa = 5.79FGDD17 pKa = 4.41CSCDD21 pKa = 3.58SIPDD25 pKa = 3.77KK26 pKa = 11.05EE27 pKa = 4.6STNMTANTLPEE38 pKa = 4.39KK39 pKa = 10.23EE40 pKa = 4.01ALARR44 pKa = 11.84AFVRR48 pKa = 11.84ILRR51 pKa = 11.84EE52 pKa = 3.83TLGDD56 pKa = 3.29EE57 pKa = 5.25DD58 pKa = 4.25YY59 pKa = 11.72AKK61 pKa = 10.78VVALNAAEE69 pKa = 4.59LNPDD73 pKa = 3.06VCASGDD79 pKa = 3.67FCDD82 pKa = 4.82SNMVMDD88 pKa = 4.97AAFKK92 pKa = 10.77EE93 pKa = 4.52FGVDD97 pKa = 3.33PLEE100 pKa = 4.16YY101 pKa = 10.37GYY103 pKa = 9.0TEE105 pKa = 4.35EE106 pKa = 6.3DD107 pKa = 3.47GMSQEE112 pKa = 5.89VCDD115 pKa = 4.6LWNSAWDD122 pKa = 3.61RR123 pKa = 11.84GKK125 pKa = 10.95EE126 pKa = 3.83LMQAGII132 pKa = 4.11

Molecular weight: 14.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9IAR8|A0A1X9IAR8_9CAUD Thymidylate synthase OS=Xanthomonas phage Xoo-sp2 OX=1852622 GN=Xoosp2_64 PE=3 SV=1
MM1 pKa = 7.87PNTARR6 pKa = 11.84TIKK9 pKa = 10.87DD10 pKa = 3.15EE11 pKa = 3.76VRR13 pKa = 11.84ILGRR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 10.01RR20 pKa = 11.84PLTYY24 pKa = 9.89PGMVDD29 pKa = 3.97AIKK32 pKa = 10.6ARR34 pKa = 11.84HH35 pKa = 6.32PDD37 pKa = 3.0ANTTVKK43 pKa = 9.46TVQWYY48 pKa = 9.39ASRR51 pKa = 11.84LRR53 pKa = 11.84RR54 pKa = 11.84EE55 pKa = 4.25GEE57 pKa = 4.0EE58 pKa = 4.15VNVKK62 pKa = 10.39DD63 pKa = 4.55GRR65 pKa = 11.84TAPEE69 pKa = 3.65RR70 pKa = 11.84AKK72 pKa = 9.9TVHH75 pKa = 6.21

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

18496

52

1399

234.1

25.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.046 ± 0.507

0.995 ± 0.131

6.553 ± 0.237

6.991 ± 0.322

3.195 ± 0.164

8.007 ± 0.22

2.044 ± 0.189

3.552 ± 0.153

5.093 ± 0.382

8.634 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.563 ± 0.112

3.011 ± 0.188

4.871 ± 0.25

4.006 ± 0.241

6.645 ± 0.315

5.536 ± 0.233

5.461 ± 0.236

7.212 ± 0.202

1.93 ± 0.173

2.655 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski