Jeotgalibacillus proteolyticus
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4022 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S5GFZ8|A0A2S5GFZ8_9BACL Cold-shock protein OS=Jeotgalibacillus proteolyticus OX=2082395 GN=C4B60_00230 PE=4 SV=1
MM1 pKa = 7.46 NISKK5 pKa = 10.7 GLLLTAAGTAILLAGCNTGGDD26 pKa = 3.67 GAEE29 pKa = 4.19 RR30 pKa = 11.84 DD31 pKa = 3.6 SSEE34 pKa = 4.8 PIVISGKK41 pKa = 9.44 PWTEE45 pKa = 3.72 QYY47 pKa = 10.75 ILPHH51 pKa = 6.74 ILQQYY56 pKa = 8.95 IEE58 pKa = 4.38 HH59 pKa = 6.72 HH60 pKa = 6.4 SDD62 pKa = 3.35 YY63 pKa = 11.17 DD64 pKa = 3.72 VEE66 pKa = 4.2 LQEE69 pKa = 4.77 GLGEE73 pKa = 4.19 VAILQPAMEE82 pKa = 4.77 NGEE85 pKa = 3.57 IDD87 pKa = 4.8 LYY89 pKa = 11.47 VEE91 pKa = 4.08 YY92 pKa = 10.21 TGTGLEE98 pKa = 4.0 AVLDD102 pKa = 4.02 EE103 pKa = 4.38 RR104 pKa = 11.84 AEE106 pKa = 4.13 EE107 pKa = 4.81 GEE109 pKa = 4.22 SSEE112 pKa = 5.15 SILDD116 pKa = 3.61 RR117 pKa = 11.84 VRR119 pKa = 11.84 QGYY122 pKa = 8.07 EE123 pKa = 3.55 EE124 pKa = 4.36 EE125 pKa = 5.59 FNITWLEE132 pKa = 3.82 PLGFANDD139 pKa = 3.33 YY140 pKa = 10.42 TMAFSEE146 pKa = 4.75 DD147 pKa = 3.47 ADD149 pKa = 4.34 FQASTYY155 pKa = 10.42 SDD157 pKa = 3.7 LVEE160 pKa = 4.62 LSSEE164 pKa = 4.21 LTFGAPHH171 pKa = 7.21 AFYY174 pKa = 10.61 EE175 pKa = 4.38 RR176 pKa = 11.84 QGDD179 pKa = 4.26 GYY181 pKa = 10.99 DD182 pKa = 4.39 AIVEE186 pKa = 4.27 AYY188 pKa = 10.12 GFDD191 pKa = 3.81 FAQTEE196 pKa = 4.34 SLDD199 pKa = 3.78 ANIMYY204 pKa = 10.19 QAVQQGQVDD213 pKa = 4.77 VIPAFTTDD221 pKa = 2.89 GRR223 pKa = 11.84 IEE225 pKa = 4.22 RR226 pKa = 11.84 FNLQTTEE233 pKa = 4.31 DD234 pKa = 3.99 DD235 pKa = 4.28 LGFFPKK241 pKa = 10.23 YY242 pKa = 9.96 DD243 pKa = 3.53 AAPVVRR249 pKa = 11.84 QDD251 pKa = 3.34 VLEE254 pKa = 4.79 AYY256 pKa = 9.44 PEE258 pKa = 4.26 LEE260 pKa = 3.91 EE261 pKa = 4.59 VLNGLAGQISEE272 pKa = 4.83 EE273 pKa = 4.7 DD274 pKa = 3.65 MQEE277 pKa = 3.67 MNAKK281 pKa = 9.81 VDD283 pKa = 3.49 IDD285 pKa = 4.21 GEE287 pKa = 4.3 DD288 pKa = 3.58 YY289 pKa = 11.34 AAVAEE294 pKa = 4.71 EE295 pKa = 4.11 FLQSKK300 pKa = 10.12 GLLDD304 pKa = 3.59
Molecular weight: 33.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.732
IPC_protein 3.719
Toseland 3.516
ProMoST 3.821
Dawson 3.681
Bjellqvist 3.859
Wikipedia 3.579
Rodwell 3.541
Grimsley 3.427
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 3.961
Thurlkill 3.554
EMBOSS 3.592
Sillero 3.834
Patrickios 0.947
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.76
Protein with the highest isoelectric point:
>tr|A0A2S5G692|A0A2S5G692_9BACL Sporulation initiation inhibitor Soj OS=Jeotgalibacillus proteolyticus OX=2082395 GN=C4B60_20800 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPNKK9 pKa = 8.24 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 SKK14 pKa = 9.83 VHH16 pKa = 5.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.19 NGRR28 pKa = 11.84 KK29 pKa = 8.36 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.8 VLSAA44 pKa = 4.11
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.522
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.735
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.457
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4022
0
4022
1171169
27
1920
291.2
32.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.402 ± 0.04
0.638 ± 0.011
5.134 ± 0.03
7.834 ± 0.051
4.569 ± 0.038
7.06 ± 0.035
2.089 ± 0.021
7.528 ± 0.041
6.391 ± 0.037
9.89 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.772 ± 0.017
4.14 ± 0.026
3.689 ± 0.021
3.685 ± 0.026
4.161 ± 0.027
6.347 ± 0.024
5.354 ± 0.023
6.866 ± 0.029
1.101 ± 0.016
3.348 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here