Escherichia phage vB_EcoS_VAH1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus; unclassified Tequintavirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 195 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482N2T6|A0A482N2T6_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_VAH1 OX=2508173 GN=VAH1_00190 PE=4 SV=1
MM1 pKa = 6.89QVYY4 pKa = 10.36VLTRR8 pKa = 11.84DD9 pKa = 3.25INEE12 pKa = 3.91YY13 pKa = 9.95NQDD16 pKa = 2.51GEE18 pKa = 4.74YY19 pKa = 9.78FVKK22 pKa = 10.56VFAEE26 pKa = 4.49KK27 pKa = 10.07PSKK30 pKa = 10.21QQLLDD35 pKa = 3.63AGVPEE40 pKa = 4.83DD41 pKa = 3.85QAKK44 pKa = 10.61CLLQDD49 pKa = 4.16KK50 pKa = 9.92EE51 pKa = 4.54FTGDD55 pKa = 3.14AYY57 pKa = 10.54EE58 pKa = 4.74CFHH61 pKa = 6.82LSCEE65 pKa = 4.56SII67 pKa = 3.59

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482N2L4|A0A482N2L4_9CAUD T5orf172 domain protein OS=Escherichia phage vB_EcoS_VAH1 OX=2508173 GN=VAH1_00110 PE=4 SV=1
MM1 pKa = 7.79AKK3 pKa = 9.77QKK5 pKa = 10.18NAKK8 pKa = 7.53TQAATTVKK16 pKa = 9.78TFPQTEE22 pKa = 4.31ANRR25 pKa = 11.84KK26 pKa = 8.9ARR28 pKa = 11.84LEE30 pKa = 3.84RR31 pKa = 11.84HH32 pKa = 5.4LRR34 pKa = 11.84KK35 pKa = 10.0HH36 pKa = 5.6PTDD39 pKa = 3.49AQAAQALNRR48 pKa = 11.84PAPIRR53 pKa = 11.84QKK55 pKa = 10.69PKK57 pKa = 10.23AKK59 pKa = 10.11NATRR63 pKa = 11.84SVARR67 pKa = 11.84LVTYY71 pKa = 9.3VQGYY75 pKa = 5.66GHH77 pKa = 7.13KK78 pKa = 9.77SVPVTLSFNAGAEE91 pKa = 4.17LFSRR95 pKa = 11.84NGMAMKK101 pKa = 10.22DD102 pKa = 3.46YY103 pKa = 10.91EE104 pKa = 4.19KK105 pKa = 10.97AVNQKK110 pKa = 9.97RR111 pKa = 11.84KK112 pKa = 8.13PTADD116 pKa = 3.19VLRR119 pKa = 11.84DD120 pKa = 3.36ARR122 pKa = 11.84GQFGSVKK129 pKa = 9.73PNIFGVEE136 pKa = 3.85YY137 pKa = 11.02SKK139 pKa = 11.81DD140 pKa = 3.37NVRR143 pKa = 11.84ALCYY147 pKa = 10.2GVGIKK152 pKa = 8.51FTGDD156 pKa = 3.33SAHH159 pKa = 7.03KK160 pKa = 9.6SAKK163 pKa = 9.35PARR166 pKa = 11.84KK167 pKa = 9.35CKK169 pKa = 10.63AKK171 pKa = 10.66

Molecular weight: 18.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

195

0

195

34289

31

1235

175.8

19.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.425 ± 0.356

1.178 ± 0.101

5.804 ± 0.17

6.906 ± 0.191

4.042 ± 0.121

6.375 ± 0.22

1.91 ± 0.13

6.664 ± 0.17

7.44 ± 0.182

8.7 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.677 ± 0.128

5.451 ± 0.153

3.433 ± 0.127

3.721 ± 0.21

4.634 ± 0.129

6.372 ± 0.218

5.751 ± 0.177

6.337 ± 0.15

1.19 ± 0.078

3.99 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski