Acidaminococcus sp. CAG:917
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1276 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R7K8C2|R7K8C2_9FIRM Uncharacterized protein OS=Acidaminococcus sp. CAG:917 OX=1262688 GN=BN810_00857 PE=4 SV=1
MM1 pKa = 7.54 TNNNDD6 pKa = 3.43 DD7 pKa = 3.61 EE8 pKa = 5.56 KK9 pKa = 11.05 EE10 pKa = 4.13 FKK12 pKa = 10.42 EE13 pKa = 4.31 LSLKK17 pKa = 10.29 EE18 pKa = 4.06 KK19 pKa = 10.51 QNLVALVASSDD30 pKa = 3.15 ADD32 pKa = 3.51 YY33 pKa = 11.33 DD34 pKa = 4.03 KK35 pKa = 11.34 KK36 pKa = 11.31 LEE38 pKa = 4.31 AYY40 pKa = 9.9 AYY42 pKa = 9.49 LCEE45 pKa = 4.37 NGEE48 pKa = 4.26 EE49 pKa = 4.43 VVDD52 pKa = 5.5 DD53 pKa = 4.4 MIKK56 pKa = 10.68 AQLTADD62 pKa = 3.93 EE63 pKa = 5.16 EE64 pKa = 4.64 SGQMLVEE71 pKa = 3.91 ILANYY76 pKa = 9.51 KK77 pKa = 9.8 GRR79 pKa = 11.84 KK80 pKa = 9.45 DD81 pKa = 3.21 IFLLLVSYY89 pKa = 8.09 FYY91 pKa = 10.72 RR92 pKa = 11.84 GEE94 pKa = 4.08 DD95 pKa = 3.14 VALFARR101 pKa = 11.84 LLGSYY106 pKa = 10.25 GDD108 pKa = 3.87 EE109 pKa = 3.94 AAIDD113 pKa = 3.68 ILKK116 pKa = 10.73 SFAKK120 pKa = 10.29 QFEE123 pKa = 4.45 DD124 pKa = 3.28 EE125 pKa = 4.28 LNYY128 pKa = 11.02 NEE130 pKa = 4.96 FMEE133 pKa = 4.25 IRR135 pKa = 11.84 NAVEE139 pKa = 3.67 EE140 pKa = 4.3 LGGDD144 pKa = 3.61 FDD146 pKa = 6.91 LNTDD150 pKa = 4.47 FSNDD154 pKa = 2.68 PFYY157 pKa = 11.02 RR158 pKa = 11.84 YY159 pKa = 10.16 LKK161 pKa = 10.2 GLDD164 pKa = 3.5 MSDD167 pKa = 3.77 LPEE170 pKa = 5.98 DD171 pKa = 4.07 EE172 pKa = 6.42 SDD174 pKa = 4.21 DD175 pKa = 4.24 CDD177 pKa = 5.7 CEE179 pKa = 4.37 EE180 pKa = 5.42 CDD182 pKa = 5.21 GNCEE186 pKa = 5.04 DD187 pKa = 6.03 DD188 pKa = 4.45 CDD190 pKa = 5.02 CDD192 pKa = 3.71 GHH194 pKa = 7.31 DD195 pKa = 4.2 GHH197 pKa = 7.69 CHH199 pKa = 7.06 DD200 pKa = 5.34 EE201 pKa = 4.04 NCGCHH206 pKa = 4.75 GHH208 pKa = 6.64 KK209 pKa = 10.32 KK210 pKa = 10.13
Molecular weight: 23.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.795
IPC2_protein 3.999
IPC_protein 3.986
Toseland 3.783
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.113
Wikipedia 3.872
Rodwell 3.821
Grimsley 3.694
Solomon 3.961
Lehninger 3.923
Nozaki 4.075
DTASelect 4.291
Thurlkill 3.821
EMBOSS 3.884
Sillero 4.113
Patrickios 1.926
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 3.991
Protein with the highest isoelectric point:
>tr|R7K9M1|R7K9M1_9FIRM Uncharacterized protein OS=Acidaminococcus sp. CAG:917 OX=1262688 GN=BN810_00041 PE=4 SV=1
MM1 pKa = 7.52 GKK3 pKa = 9.83 GAKK6 pKa = 9.09 KK7 pKa = 10.41 DD8 pKa = 3.6 YY9 pKa = 10.36 FGLGRR14 pKa = 11.84 IVSIILAIIPVTAWLLGAITRR35 pKa = 11.84 IMDD38 pKa = 3.94 GGLGIVFGIIRR49 pKa = 11.84 LIGGGFILWIIDD61 pKa = 4.77 LILMIVRR68 pKa = 11.84 GSILRR73 pKa = 11.84 IAA75 pKa = 4.19
Molecular weight: 8.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.305
IPC2_protein 9.882
IPC_protein 10.906
Toseland 11.111
ProMoST 11.111
Dawson 11.155
Bjellqvist 10.95
Wikipedia 11.447
Rodwell 11.286
Grimsley 11.199
Solomon 11.418
Lehninger 11.359
Nozaki 11.082
DTASelect 10.95
Thurlkill 11.096
EMBOSS 11.535
Sillero 11.111
Patrickios 11.096
IPC_peptide 11.418
IPC2_peptide 10.028
IPC2.peptide.svr19 8.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1276
0
1276
414890
30
6614
325.1
36.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.504 ± 0.07
1.557 ± 0.039
6.116 ± 0.059
6.96 ± 0.068
4.683 ± 0.045
6.864 ± 0.06
1.285 ± 0.03
7.408 ± 0.053
8.415 ± 0.107
8.819 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.446 ± 0.037
4.724 ± 0.062
3.082 ± 0.039
2.439 ± 0.03
3.557 ± 0.061
6.606 ± 0.069
5.439 ± 0.089
7.368 ± 0.054
0.627 ± 0.021
4.09 ± 0.079
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here