Trichinella murrelli
Average proteome isoelectric point is 7.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17983 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0V0U2Q7|A0A0V0U2Q7_9BILA Thioredoxin domain-containing protein 17 OS=Trichinella murrelli OX=144512 GN=ATE1 PE=3 SV=1
MM1 pKa = 7.71 EE2 pKa = 5.35 DD3 pKa = 2.95 WEE5 pKa = 4.84 IFDD8 pKa = 5.02 FDD10 pKa = 4.01 VDD12 pKa = 3.84 LMLWLVVLVILLHH25 pKa = 4.69 MAKK28 pKa = 10.38 YY29 pKa = 10.55 LLDD32 pKa = 3.73 YY33 pKa = 11.44 LEE35 pKa = 4.54 TFQEE39 pKa = 4.26 KK40 pKa = 10.83 EE41 pKa = 3.96 EE42 pKa = 4.85 LIQLLNEE49 pKa = 3.94 THH51 pKa = 6.55 YY52 pKa = 11.81 SMQQVEE58 pKa = 4.75 QIVEE62 pKa = 4.18 HH63 pKa = 5.41 QQSVDD68 pKa = 3.12 NKK70 pKa = 10.54 DD71 pKa = 4.01 YY72 pKa = 11.01 IGEE75 pKa = 4.15 PYY77 pKa = 10.21 IFYY80 pKa = 10.94 DD81 pKa = 3.27 PDD83 pKa = 4.24 KK84 pKa = 11.38 DD85 pKa = 4.21 VFTLQIDD92 pKa = 4.34 FNNLSEE98 pKa = 4.58 DD99 pKa = 3.47 NCIKK103 pKa = 10.69 VCLAVWNALVNIDD116 pKa = 4.99 SEE118 pKa = 4.61 DD119 pKa = 3.61 DD120 pKa = 3.76 SEE122 pKa = 5.38 EE123 pKa = 4.4 SDD125 pKa = 3.61 EE126 pKa = 4.16 QQ127 pKa = 3.8
Molecular weight: 15.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.656
IPC_protein 3.643
Toseland 3.439
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.554
Rodwell 3.478
Grimsley 3.35
Solomon 3.617
Lehninger 3.567
Nozaki 3.757
DTASelect 3.948
Thurlkill 3.49
EMBOSS 3.567
Sillero 3.77
Patrickios 0.655
IPC_peptide 3.617
IPC2_peptide 3.745
IPC2.peptide.svr19 3.699
Protein with the highest isoelectric point:
>tr|A0A0V0SZ18|A0A0V0SZ18_9BILA Uncharacterized protein OS=Trichinella murrelli OX=144512 GN=T05_1117 PE=4 SV=1
SS1 pKa = 5.89 RR2 pKa = 11.84 TTRR5 pKa = 11.84 SGSRR9 pKa = 11.84 TTRR12 pKa = 11.84 SRR14 pKa = 11.84 SRR16 pKa = 11.84 TTRR19 pKa = 11.84 SRR21 pKa = 11.84 SRR23 pKa = 11.84 TTRR26 pKa = 11.84 SRR28 pKa = 11.84 SRR30 pKa = 11.84 TTRR33 pKa = 11.84 SRR35 pKa = 3.15
Molecular weight: 4.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.33
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.618
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.34
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.277
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14367
3616
17983
8302106
30
15251
461.7
52.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.457 ± 0.017
2.636 ± 0.017
5.242 ± 0.013
6.333 ± 0.024
4.575 ± 0.014
4.979 ± 0.02
2.466 ± 0.007
5.563 ± 0.013
6.027 ± 0.02
9.67 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.418 ± 0.008
4.993 ± 0.012
4.331 ± 0.018
4.263 ± 0.016
5.666 ± 0.018
8.361 ± 0.018
5.315 ± 0.014
6.418 ± 0.014
1.211 ± 0.005
3.073 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here